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WO2003066896A3 - Procede de detection de mutations - Google Patents

Procede de detection de mutations Download PDF

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Publication number
WO2003066896A3
WO2003066896A3 PCT/EP2003/001258 EP0301258W WO03066896A3 WO 2003066896 A3 WO2003066896 A3 WO 2003066896A3 EP 0301258 W EP0301258 W EP 0301258W WO 03066896 A3 WO03066896 A3 WO 03066896A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligos
linkage
complex
mutations
analysis
Prior art date
Application number
PCT/EP2003/001258
Other languages
German (de)
English (en)
Other versions
WO2003066896A2 (fr
Inventor
Christian Albrecht
Filipp Oesterhelt
Boris Steipe
Hermann Gaub
Hauke Clausen-Schaumann
Michael Cieplik
Original Assignee
Nanotype Gmbh
Christian Albrecht
Filipp Oesterhelt
Boris Steipe
Hermann Gaub
Hauke Clausen-Schaumann
Michael Cieplik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2002105419 external-priority patent/DE10205419A1/de
Priority claimed from DE2002107918 external-priority patent/DE10207918A1/de
Application filed by Nanotype Gmbh, Christian Albrecht, Filipp Oesterhelt, Boris Steipe, Hermann Gaub, Hauke Clausen-Schaumann, Michael Cieplik filed Critical Nanotype Gmbh
Priority to EP03737328A priority Critical patent/EP1565569A2/fr
Priority to AU2003244501A priority patent/AU2003244501A1/en
Publication of WO2003066896A2 publication Critical patent/WO2003066896A2/fr
Publication of WO2003066896A3 publication Critical patent/WO2003066896A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de détection de mutations dans un acide nucléique, consistant a) à mettre en contact une pluralité de brins d'acide nucléique à analyser avec au moins deux types d'oligonucléotides, des récepteurs oligos et des sondes oligos, dans des conditions telles que ces oligos s'hybrident avec l'ADN à analyser en formant un complexe sonde et un complexe de référence, de sorte qu'une liaison immobilisée ou immobilisable des deux côtés se forme entre lesdits complexes sonde et de référence, b) à immobiliser ensuite éventuellement ladite liaison, c) à appliquer une force de traction sur cette liaison, jusqu'à ce qu'un des complexes se sépare et d) à déterminer lequel de ces complexes s'est séparé.
PCT/EP2003/001258 2002-02-09 2003-02-07 Procede de detection de mutations WO2003066896A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP03737328A EP1565569A2 (fr) 2002-02-09 2003-02-07 Procede de detection de mutations
AU2003244501A AU2003244501A1 (en) 2002-02-09 2003-02-07 Method for the detection of mutations

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE2002105419 DE10205419A1 (de) 2002-02-09 2002-02-09 Verfahren zum Nachweis von Mutationen
DE10205419.3 2002-02-09
DE10207918.8 2002-02-23
DE2002107918 DE10207918A1 (de) 2002-02-23 2002-02-23 Verfahren zum Nachweis von Mutationen

Publications (2)

Publication Number Publication Date
WO2003066896A2 WO2003066896A2 (fr) 2003-08-14
WO2003066896A3 true WO2003066896A3 (fr) 2004-03-25

Family

ID=27735663

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/001258 WO2003066896A2 (fr) 2002-02-09 2003-02-07 Procede de detection de mutations

Country Status (3)

Country Link
EP (1) EP1565569A2 (fr)
AU (1) AU2003244501A1 (fr)
WO (1) WO2003066896A2 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10126798A1 (de) 2001-06-01 2002-12-19 Nanotype Gmbh Verfahren zur Bestimmung einer Probe
KR100580644B1 (ko) 2004-02-16 2006-05-16 삼성전자주식회사 생물분자를 고체 기판상에 비공유적으로 고정화 하는 방법및 그에 의하여 제조되는 마이크로어레이
EP2390351A1 (fr) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Procédé de séquençage de l'ADN par hybridisation
EP2390350A1 (fr) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Procédé de séquençage de l'ADN par polymérisation
FR2993282B1 (fr) 2012-07-13 2017-11-10 Expanscience Lab Procede d'identification de marqueurs moleculaires de la peau d'enfant
FR3001889B1 (fr) 2013-02-11 2021-02-12 Expanscience Lab Utilisation d'une composition comprenant un perseose d'avocat dans la protection des cellules souches epidermiques .
FR3011008B1 (fr) 2013-09-24 2017-12-29 Expanscience Lab Procedes d'evaluation des effets deleteres des uv sur la peau d'enfant
EP2886663A1 (fr) 2013-12-19 2015-06-24 Centre National de la Recherche Scientifique (CNRS) Séquençage de nanopore utilisant des polymérases réplicatifs et hélicases
FR3016373B1 (fr) 2014-01-10 2018-01-19 Laboratoires Expanscience Modele de peau de mammelon reconstitue
FR3019186B1 (fr) 2014-03-31 2019-06-07 Laboratoires Expanscience Procedes d'evaluation des effets deleteres de l'urine sur la peau d'enfant
FR3045669B1 (fr) 2015-12-16 2019-04-05 Laboratoires Expanscience Procedes d'evaluation des effets de la deshydratation sur la peau d'enfant
FR3053053B1 (fr) 2016-06-23 2018-07-13 Laboratoires Expanscience Modeles de la dermatite atopique juvenile
FR3059550B1 (fr) 2016-12-01 2020-01-03 Universite De Rouen Normandie Traitement des troubles causes par l'alcoolisation foetale (tcaf)
FR3068045B1 (fr) 2017-06-22 2021-06-04 Expanscience Lab Modeles de peau sensible reconstituee
FR3081707A1 (fr) 2018-05-30 2019-12-06 Universite De Rouen Normandie Traitement des troubles neurologiques causes par l'alcoolisation foetale
MX2021000786A (es) 2018-07-20 2021-06-15 Pf Medicament Receptor para supresor de ig del dominio v de activación de células t (vista).
FR3091351B1 (fr) 2018-12-27 2021-05-21 Univ Rouen Centre Hospitalier Biomarqueur de la maladie de fabry
FR3098405B1 (fr) 2019-07-12 2021-06-25 Expanscience Lab Composition comprenant des polyphénols de graines de passiflore, des peptides d’avocat et un extrait d’hamamélis et utilisation pour traiter et/ou prévenir les vergetures
EP3922641A1 (fr) 2020-06-09 2021-12-15 Institut Gustave-Roussy Procédés de détection et de traitement de patients covid nécessitant des soins intensifs
FR3133198A1 (fr) 2022-03-04 2023-09-08 Pierre Fabre Dermo-Cosmetique Modele de peau reconstituee

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6342353B1 (en) * 1997-02-21 2002-01-29 Centre National De La Recherche Scientifique (Cnrs) Method for characterizing nucleic acid duplex
WO2002014862A2 (fr) * 2000-08-11 2002-02-21 Nanotype Gmbh Procede et dispositif de caracterisation et/ou d'identification d'un complexe de liaison

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6342353B1 (en) * 1997-02-21 2002-01-29 Centre National De La Recherche Scientifique (Cnrs) Method for characterizing nucleic acid duplex
WO2002014862A2 (fr) * 2000-08-11 2002-02-21 Nanotype Gmbh Procede et dispositif de caracterisation et/ou d'identification d'un complexe de liaison

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ALBRECHT CHRISTIAN ET AL: "DNA: a programmable force sensor.", SCIENCE. UNITED STATES 18 JUL 2003, vol. 301, no. 5631, 18 July 2003 (2003-07-18), pages 367 - 370, XP002256755, ISSN: 1095-9203 *
NOY A ET AL: "STRETCHING AND BREAKING DUPLEX DNA BY CHEMICAL FORCE MICROSCOPY", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 4, no. 7, 1 July 1997 (1997-07-01), pages 519 - 527, XP002071033, ISSN: 1074-5521 *
STRUNZ TORSTEN ET AL: "Dynamic force spectroscopy of single DNA molecules", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 20, 28 September 1999 (1999-09-28), Sept. 28, 1999, pages 11277 - 11282, XP002256754, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2003066896A2 (fr) 2003-08-14
AU2003244501A1 (en) 2003-09-02
EP1565569A2 (fr) 2005-08-24

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