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WO2003002730A3 - Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite methode - Google Patents

Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite methode Download PDF

Info

Publication number
WO2003002730A3
WO2003002730A3 PCT/FR2002/002178 FR0202178W WO03002730A3 WO 2003002730 A3 WO2003002730 A3 WO 2003002730A3 FR 0202178 W FR0202178 W FR 0202178W WO 03002730 A3 WO03002730 A3 WO 03002730A3
Authority
WO
WIPO (PCT)
Prior art keywords
activity
biological sample
peptides
implementing
calpain
Prior art date
Application number
PCT/FR2002/002178
Other languages
English (en)
Other versions
WO2003002730A2 (fr
Inventor
Isabelle Richard
Guillaume Sillon
Original Assignee
Genethon
Centre Nat Rech Scient
Isabelle Richard
Guillaume Sillon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genethon, Centre Nat Rech Scient, Isabelle Richard, Guillaume Sillon filed Critical Genethon
Priority to US10/432,688 priority Critical patent/US20040180395A1/en
Priority to CA002429429A priority patent/CA2429429A1/fr
Priority to JP2003509092A priority patent/JP2004520850A/ja
Priority to AU2002321389A priority patent/AU2002321389B2/en
Priority to EP02755089A priority patent/EP1399545A2/fr
Publication of WO2003002730A2 publication Critical patent/WO2003002730A2/fr
Publication of WO2003002730A3 publication Critical patent/WO2003002730A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Peptide couplé à au moins une molécule rapporteuse fluorogénique ou colorogénique, caractérisé en ce qu'il contient au moins une séquence d'acides aminés apte à être clivée par la calpaine 3. Méthode de détection in vitro de l'activité de la calpaine 3 dans un échantillon biologique.
PCT/FR2002/002178 2001-06-29 2002-06-24 Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite methode WO2003002730A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/432,688 US20040180395A1 (en) 2001-06-29 2002-06-24 Method for detecting calpain3 activity in a biological sample and peptides for implementing said method
CA002429429A CA2429429A1 (fr) 2001-06-29 2002-06-24 Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite methode
JP2003509092A JP2004520850A (ja) 2001-06-29 2002-06-24 生体試料のカルパイン３活性を検出する方法、および前記方法を実施するためのペプチド
AU2002321389A AU2002321389B2 (en) 2001-06-29 2002-06-24 Method for detecting calpain 3 activity in a biological sample and peptides for implementing said method
EP02755089A EP1399545A2 (fr) 2001-06-29 2002-06-24 Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite mehtode

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR01/08614 2001-06-29
FR0108614A FR2826656B1 (fr) 2001-06-29 2001-06-29 Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite methode

Publications (2)

Publication Number Publication Date
WO2003002730A2 WO2003002730A2 (fr) 2003-01-09
WO2003002730A3 true WO2003002730A3 (fr) 2003-12-11

Family

ID=8864926

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/002178 WO2003002730A2 (fr) 2001-06-29 2002-06-24 Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite methode

Country Status (8)

Country Link
US (1) US20040180395A1 (fr)
EP (1) EP1399545A2 (fr)
JP (1) JP2004520850A (fr)
CN (1) CN1514878A (fr)
AU (1) AU2002321389B2 (fr)
CA (1) CA2429429A1 (fr)
FR (1) FR2826656B1 (fr)
WO (1) WO2003002730A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4803976B2 (ja) * 2003-07-09 2011-10-26 独立行政法人科学技術振興機構 細胞内ｉｐ３測定用分子センサー
FR2858177A1 (fr) * 2003-07-28 2005-02-04 Genethon Utilisation du phenomeme fret, detecte par mplsm, pour le suivi in vivo d'evenements biologiques
JP2007049943A (ja) * 2005-08-18 2007-03-01 Kyoto Univ 細胞内カルシウムイオン指示機能を有するポリペプチド
FR2891544A1 (fr) * 2005-09-30 2007-04-06 Genethon Ass Loi De 1901 Substrat proteique pour la detection de l'activite calpaine 3
FR2962041B1 (fr) 2010-07-01 2012-07-27 Genethon Inhibiteurs de la calpaine 3 pour le traitement de dystrophies musculaires et de cardiomyopathies
CN102154288B (zh) * 2010-12-21 2012-12-19 山东农业大学 一种骨骼肌特异性capn3启动子及其应用
US20160102336A1 (en) * 2013-03-04 2016-04-14 The University Of Tokyo Fluorescent probe for detecting activity of calpain
CN104655596A (zh) * 2013-11-18 2015-05-27 李捷 一种含红细胞血液样品的质量检测方法及检测试剂盒
EP3987050A1 (fr) * 2019-06-24 2022-04-27 Urteste S.A. Nouveau marqueur de diagnostic pour le cancer du pancréas
EP4361166A4 (fr) * 2021-06-24 2025-07-16 Tohoku Techno Arch Co Ltd Sondes fluorescentes
CN115356314B (zh) * 2022-08-24 2025-04-15 暨南大学 基于荧光传感器的钙蛋白酶活性检测方法及应用
WO2024237099A1 (fr) * 2023-05-12 2024-11-21 五稜化薬株式会社 Préparation lyophilisée, solution lyophilisée, leurs procédés de production et agent liquide

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19650142A1 (de) * 1996-12-04 1998-06-10 Basf Ag Neue Calpaine, ihre Herstellung und Verwendung

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19650142A1 (de) * 1996-12-04 1998-06-10 Basf Ag Neue Calpaine, ihre Herstellung und Verwendung

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KINBARA K ET AL: "Purification of native p94, a muscle-specific calpain, and characterization of its autolysis.", THE BIOCHEMICAL JOURNAL. ENGLAND 1 NOV 1998, vol. 335 ( Pt 3), 1 November 1998 (1998-11-01), pages 589 - 596, XP002238589, ISSN: 0264-6021 *
ONO Y ET AL: "Functional defects of a muscle-specific calpain, p94, caused by mutations associated with limb-girdle muscular dystrophy type 2A.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 3 JUL 1998, vol. 273, no. 27, 3 July 1998 (1998-07-03), pages 17073 - 17078, XP002238594, ISSN: 0021-9258 *
VANDERKLISH PETER W ET AL: "Marking synaptic activity in dendritic spines with a calpain substrate exhibiting fluorescence resonance energy transfer.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 97, no. 5, 29 February 2000 (2000-02-29), Feb. 29, 2000, pages 2253 - 2258, XP002194684, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
CN1514878A (zh) 2004-07-21
FR2826656B1 (fr) 2003-09-12
WO2003002730A2 (fr) 2003-01-09
EP1399545A2 (fr) 2004-03-24
CA2429429A1 (fr) 2003-01-09
US20040180395A1 (en) 2004-09-16
AU2002321389B2 (en) 2005-03-24
FR2826656A1 (fr) 2003-01-03
JP2004520850A (ja) 2004-07-15

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