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WO2003031654A1 - Microreseau comprenant des sondes pour genotyper des especes de mycobacteries, pour differencier des souches de bacille de koch et pour detecter des souches resistantes aux antibiotiques - Google Patents

Microreseau comprenant des sondes pour genotyper des especes de mycobacteries, pour differencier des souches de bacille de koch et pour detecter des souches resistantes aux antibiotiques Download PDF

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Publication number
WO2003031654A1
WO2003031654A1 PCT/KR2002/001885 KR0201885W WO03031654A1 WO 2003031654 A1 WO2003031654 A1 WO 2003031654A1 KR 0201885 W KR0201885 W KR 0201885W WO 03031654 A1 WO03031654 A1 WO 03031654A1
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WIPO (PCT)
Prior art keywords
tuberculosis
strains
probe
probes
microarray
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PCT/KR2002/001885
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English (en)
Inventor
Cheol-Min Kim
Hee-Kyung Park
Hyun-Jung Jang
Eun-Sil Song
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Sj Hightech Co.,Ltd.
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Publication of WO2003031654A1 publication Critical patent/WO2003031654A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Definitions

  • the probes or primers used in the present invention for genotyping Mycobacteria species, differentiating M. tuberculosis strains, and detecting antibiotic-resistant strains are shown in Tables 1 , 2, and 3 below, respectively.
  • kat4R 5'-biotin-CTG CAG GCG GAT GCG ACC A-3' (SEQ ID NO. 164) were prepared for PCR of the codon 463 region.
  • the standard M. tuberculosis H37Rv strain, INH-resistant M. tuberculosis clinical isolates, and INH-susceptible M. tuberculosis clinical isolates were amplified using the primers by PCR.
  • the M. bovis strain hybridized only to the probes SPO-01 , SPO-03, SPO-04, SPO-05, SPO-06, SPO-07, SPO-08, SPO-11 , SPO-12, SPO-12, SPO-13, SPO-17, SPO-18, SPO-19, SPO-20, SPO-21, SPO-22, SPO-23, SPO-24, SPO-25, SPO-26, SPO-27, SPO-28, SPO-29, SPO-30, SPO-31 , SPO-34, SPO-35, SPO-37, and SPO-38 in spots 50, 52, 53, 54, 55, 56, 57, 60, 61, 62, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 83, 84, 86, and 87 of FIG. 1 , respectively.
  • FIGS. 5C and 5D show the results of an INH-resistance test when M. tuberculosis H37Rv strain that is susceptible to INH and an INH-resistant mutant with the substitution of threonine (ACC) for serine (AGC) at codon 315 of the katG gene were used as the target DNA, respectively.
  • hybridization occurred in the positive control probe which is a mixture of the individual probes for both strains.
  • the INH-susceptible M. tuberculosis hybridized only to the wild-type probes for codons 315 and 463, i.e., kat 315-WS and kat 463-WR in spots 44 and 47 of FIG. 1 , respectively.
  • the INH-resistant mutant hybridized specifically to the probes kat 315-MT and kat 463-WR in spots 45 and 47 of FIG. 1 , respectively.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un microréseau comprenant des sondes pour génotyper des espèces de mycobactéries, pour différencier des souches de bacille de Koch et pour détecter des souches résistantes aux antibiotiques, immobilisées sur un support, un procédé pour génotyper des espèces de mycobactéries, pour différencier des souches de bacille de Koch et pour détecter des souches résistantes aux antibiotiques en mettant en oeuvre ledit microréseau, ainsi qu'une trousse de diagnostic pour des infections à mycobactéries induisant un tuberculose et n'induisant pas une tuberculose, qui met en oeuvre le microréseau. Ce microréseau comprend des sondes pour génotyper des espèces de mycobactéries, des sondes pour différencier des souches de bacille de Koch et des sondes pour détecter des souches résistantes aux antibiotiques. Lorsqu'une pluralité de ces ensembles de sondes sont immobilisés sur un seul support, on peut réaliser simultanément, en un seul test, un test pour génotyper des mycobactéries, un test pour différencier des souches de bacille de Koch et un test pour détecter une résistance aux antibiotiques, sur plusieurs isolats cliniques, réduisant ainsi la perte de temps et les coûts. Des isolats cliniques et des cultures de souches peuvent être appliqués directement sur le microréseau, réduisant encore le temps nécessaire à un diagnostic. De plus, les résultats des tests selon cette invention sont précis.
PCT/KR2002/001885 2001-10-09 2002-10-09 Microreseau comprenant des sondes pour genotyper des especes de mycobacteries, pour differencier des souches de bacille de koch et pour detecter des souches resistantes aux antibiotiques WO2003031654A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR2001/62125 2001-10-09
KR10-2001-0062125A KR100454585B1 (ko) 2001-10-09 2001-10-09 마이코박테리아의 균주 감별, 결핵균의 스트레인 감별과항생제 내성을 검출하기 위한 프로브를 포함하는마이크로어레이와 이를 이용한 검출 방법 및 진단 키트

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WO2003031654A1 true WO2003031654A1 (fr) 2003-04-17

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KR (1) KR100454585B1 (fr)
WO (1) WO2003031654A1 (fr)

Cited By (13)

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FR2860801A1 (fr) * 2003-10-10 2005-04-15 Bertin Technologies Sa Methode de detection rapide de micro-organismes sur puces a adn
WO2005078131A1 (fr) * 2004-02-11 2005-08-25 Health Protection Agency Dosage de resistance a tb
EP1634959A3 (fr) * 2004-09-14 2006-03-22 Samsung Electronics Co.,Ltd. Procédé de conception d'un jeu de sondes, micro-réseau l'utilisant, support lisible par ordinateur muni d'un programme pour exécuter ladite méthode
EP1580281A3 (fr) * 2003-09-29 2006-09-13 Eppendorf Ag Procédé pour determiner la résistance microbienne aux antibiotiques
WO2006097232A3 (fr) * 2005-03-18 2007-02-01 Eppendorf Ag Detection d'une sensibilite aux antibiotiques et de facteurs de virulence chez pseudomonas aeruginosa
WO2007018563A3 (fr) * 2004-10-05 2007-06-21 Wyeth Corp Ensembles de sondes de detection de multiples souches de differentes especes
US7332597B2 (en) 2004-06-28 2008-02-19 University Of Kentucky Research Foundation Primers and probe to identify mycobacterium tuberculosis complex
CN1995380B (zh) * 2006-01-05 2010-05-12 中国人民解放军总医院第二附属医院 一种检测和鉴定分枝杆菌菌种的方法及其专用试剂盒
EP2179041A4 (fr) * 2007-06-22 2010-12-22 Ibis Biosciences Inc Compositions et procédés permettant d'identifier des caractéristiques de sous-espèces de mycobacterium tuberculosis
JP2013502922A (ja) * 2009-08-26 2013-01-31 エルジー ライフ サイエンス リミテッド Ｍ．ツベルクローシス（ｍ．ｔｕｂｅｒｃｕｌｏｓｉｓ）群、およびマイコバクテリア（ｍｙｃｏｂａｃｔｅｒｉａ）属を検出するための組成物と、同組成物を用いたマルチプレックスリアルタイムｐｃｒによりｍ．ツベルクローシス（ｍ．ｔｕｂｅｒｃｕｌｏｓｉｓ）群、およびマイコバクテリア（ｍｙｃｏｂａｃｔｅｒｉａ）属を同時に検出する方法
EP2526208A4 (fr) * 2010-01-21 2014-01-15 Sequella Inc Méthodes et compositions améliorées pour la détermination du statut pathogène d'agents infectieux
RU2715591C1 (ru) * 2016-08-04 2020-03-02 Оптифарм.Ко., Лтд Диагностический способ и набор для одновременного обнаружения и идентификации туберкулезной микобактерии и нетуберкулезных микобактерий и определения рифампициновой устойчивости туберкулезной микобактерии на основе аналитической платформы quantamatrix
CN112933952A (zh) * 2021-02-02 2021-06-11 东莞市科伟环保电力有限公司 一种基于大数据的pncr脱硝优化系统

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KR101155973B1 (ko) * 2009-07-07 2012-06-18 엠앤디 (주) 결핵균 약제 내성 진단용 프로브 및 그 키트
EP2816123B1 (fr) 2010-02-24 2018-09-12 The Broad Institute, Inc. Méthodes de diagnostic des agents pathogènes de maladies infectieuses et leur sensibilité aux médicaments
KR101492561B1 (ko) 2012-05-25 2015-02-11 경상대학교산학협력단 항생제 내성균 검출용 마이크로어레이 및 이를 이용한 항생제 내성균 분석 방법
CN109797193A (zh) * 2019-02-15 2019-05-24 安徽理工大学 一种结核分枝杆菌耐药性检测方法
KR102247643B1 (ko) * 2019-11-01 2021-05-03 바이오파크진단 주식회사 결핵균 및 비결핵항산균을 검출하기 위한 2단계 진단방법 및 키트
KR102524159B1 (ko) * 2020-10-26 2023-04-24 한양대학교 에리카산학협력단 결핵, 비결핵 항산균 감염질환 및 잠복결핵 진단용 바이오마커 및 이의 용도

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EP1076099A2 (fr) * 1999-08-03 2001-02-14 Nisshinbo Industries, Inc. Trousse pour la diagnose des bacilles de tubercule
KR20030008620A (ko) * 2001-07-19 2003-01-29 주식회사 제니스라이프사이언스 마이코박테리아의 동정 및 ｒｐｏＢ 유전자의 돌연변이에의해 얻어지는 항 결핵제 내성의 동시 검출방법

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Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1580281A3 (fr) * 2003-09-29 2006-09-13 Eppendorf Ag Procédé pour determiner la résistance microbienne aux antibiotiques
WO2005035785A3 (fr) * 2003-10-10 2006-04-20 Bertin Technologies Sa Methode de detection rapide de micro-organismes sur puces a adn
FR2860801A1 (fr) * 2003-10-10 2005-04-15 Bertin Technologies Sa Methode de detection rapide de micro-organismes sur puces a adn
JP2007521829A (ja) * 2004-02-11 2007-08-09 ヘルス プロテクション エージェンシー Ｔｂ耐性アッセイ
WO2005078131A1 (fr) * 2004-02-11 2005-08-25 Health Protection Agency Dosage de resistance a tb
AU2005213527B2 (en) * 2004-02-11 2010-05-20 The Secretary Of State For Health TB resistance assay
US7332597B2 (en) 2004-06-28 2008-02-19 University Of Kentucky Research Foundation Primers and probe to identify mycobacterium tuberculosis complex
US7657381B2 (en) 2004-09-14 2010-02-02 Samsung Electronics Co., Ltd. Method of designing probe set, microarray having substrate on which probe designed by the method is immobilized, and computer readable medium on which program for executing the method is recorded
EP1634959A3 (fr) * 2004-09-14 2006-03-22 Samsung Electronics Co.,Ltd. Procédé de conception d'un jeu de sondes, micro-réseau l'utilisant, support lisible par ordinateur muni d'un programme pour exécuter ladite méthode
WO2007018563A3 (fr) * 2004-10-05 2007-06-21 Wyeth Corp Ensembles de sondes de detection de multiples souches de differentes especes
WO2006097232A3 (fr) * 2005-03-18 2007-02-01 Eppendorf Ag Detection d'une sensibilite aux antibiotiques et de facteurs de virulence chez pseudomonas aeruginosa
CN1995380B (zh) * 2006-01-05 2010-05-12 中国人民解放军总医院第二附属医院 一种检测和鉴定分枝杆菌菌种的方法及其专用试剂盒
EP2179041A4 (fr) * 2007-06-22 2010-12-22 Ibis Biosciences Inc Compositions et procédés permettant d'identifier des caractéristiques de sous-espèces de mycobacterium tuberculosis
JP2013502922A (ja) * 2009-08-26 2013-01-31 エルジー ライフ サイエンス リミテッド Ｍ．ツベルクローシス（ｍ．ｔｕｂｅｒｃｕｌｏｓｉｓ）群、およびマイコバクテリア（ｍｙｃｏｂａｃｔｅｒｉａ）属を検出するための組成物と、同組成物を用いたマルチプレックスリアルタイムｐｃｒによりｍ．ツベルクローシス（ｍ．ｔｕｂｅｒｃｕｌｏｓｉｓ）群、およびマイコバクテリア（ｍｙｃｏｂａｃｔｅｒｉａ）属を同時に検出する方法
EP2526208A4 (fr) * 2010-01-21 2014-01-15 Sequella Inc Méthodes et compositions améliorées pour la détermination du statut pathogène d'agents infectieux
RU2715591C1 (ru) * 2016-08-04 2020-03-02 Оптифарм.Ко., Лтд Диагностический способ и набор для одновременного обнаружения и идентификации туберкулезной микобактерии и нетуберкулезных микобактерий и определения рифампициновой устойчивости туберкулезной микобактерии на основе аналитической платформы quantamatrix
CN112933952A (zh) * 2021-02-02 2021-06-11 东莞市科伟环保电力有限公司 一种基于大数据的pncr脱硝优化系统

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