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WO2003034044A3 - Multi-color multiplexed analysis in a bio-separation system - Google Patents

Multi-color multiplexed analysis in a bio-separation system Download PDF

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Publication number
WO2003034044A3
WO2003034044A3 PCT/US2002/033684 US0233684W WO03034044A3 WO 2003034044 A3 WO2003034044 A3 WO 2003034044A3 US 0233684 W US0233684 W US 0233684W WO 03034044 A3 WO03034044 A3 WO 03034044A3
Authority
WO
WIPO (PCT)
Prior art keywords
radiation
detection
radiation sources
bio
sources
Prior art date
Application number
PCT/US2002/033684
Other languages
French (fr)
Other versions
WO2003034044A2 (en
Inventor
Varouj Amirkhanian
Ming-Sun Liu
Original Assignee
Biocal Technology Inc
Varouj Amirkhanian
Ming-Sun Liu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocal Technology Inc, Varouj Amirkhanian, Ming-Sun Liu filed Critical Biocal Technology Inc
Priority to AU2002359285A priority Critical patent/AU2002359285A1/en
Publication of WO2003034044A2 publication Critical patent/WO2003034044A2/en
Publication of WO2003034044A3 publication Critical patent/WO2003034044A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A multi-channel and multi-color bio-separation detection method and apparatus in which a single detector (450) is coupled to a plurality of radiation sources (420,433), in a one detector/many radiation sources configuration. Each radiation source directs radiation at a detection zone (406) of a single separation channel (140), and a single detector is applied to detect ligh emissions from the detection zones associated with several radiation sources. The radiation sources are activated to direct radiation at the detection zone in a predetermined sequence and further in a cyclic manner, with the detector output synchronized to the radiation sources by a controller (300). Bio-separation may be conducted simultaneously in all the channels in parallel, with detection time-staggered and/or time multiplexed with respect to the light sources. In one embodiment, low cost light emitting diodes may be used as radiation sources. In another aspect, the detection scheme is configured for radiation induced fluorescence detection in an capillary electrophoresis instrument.
PCT/US2002/033684 2001-10-19 2002-10-21 Multi-color multiplexed analysis in a bio-separation system WO2003034044A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002359285A AU2002359285A1 (en) 2001-10-19 2002-10-21 Multi-color multiplexed analysis in a bio-separation system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34803401P 2001-10-19 2001-10-19
US60/348,034 2001-10-19

Publications (2)

Publication Number Publication Date
WO2003034044A2 WO2003034044A2 (en) 2003-04-24
WO2003034044A3 true WO2003034044A3 (en) 2003-08-14

Family

ID=23366378

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/033684 WO2003034044A2 (en) 2001-10-19 2002-10-21 Multi-color multiplexed analysis in a bio-separation system

Country Status (2)

Country Link
AU (1) AU2002359285A1 (en)
WO (1) WO2003034044A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846315B2 (en) 2002-01-28 2010-12-07 Qiagen Sciences, Llc Integrated bio-analysis and sample preparation system
WO2005040331A1 (en) * 2003-10-24 2005-05-06 Egene, Inc. Integrated bio-analysis and sample preparation system
CN103266918B (en) * 2013-05-23 2015-09-30 山西煤炭运销集团有限公司 The parallel direct-reading roof separation indicator of a kind of networking numeral
CN103266919B (en) * 2013-05-23 2015-09-30 山西煤炭运销集团有限公司 A kind of networking numeral straight-bar direct-reading roof separation indicator
CN106018403B (en) * 2016-05-12 2019-05-21 南京擎科生物科技有限公司 The light absorption detector and detection method of capillary array electrophoresis instrument
EP4544289A1 (en) * 2022-06-24 2025-04-30 Gen-Probe Incorporated Compact detection system

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
EP0723149A2 (en) * 1995-01-19 1996-07-24 Hitachi, Ltd. Capillary array electrophoresis system
WO2001002846A1 (en) * 1999-07-01 2001-01-11 Corning Applied Technologies, Incorporated System and method for molecular sample measurement

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
EP0723149A2 (en) * 1995-01-19 1996-07-24 Hitachi, Ltd. Capillary array electrophoresis system
WO2001002846A1 (en) * 1999-07-01 2001-01-11 Corning Applied Technologies, Incorporated System and method for molecular sample measurement

Also Published As

Publication number Publication date
WO2003034044A2 (en) 2003-04-24
AU2002359285A1 (en) 2003-04-28

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