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WO2003035829A3 - Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules - Google Patents

Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules Download PDF

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Publication number
WO2003035829A3
WO2003035829A3 PCT/US2002/032088 US0232088W WO03035829A3 WO 2003035829 A3 WO2003035829 A3 WO 2003035829A3 US 0232088 W US0232088 W US 0232088W WO 03035829 A3 WO03035829 A3 WO 03035829A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
methods
nucleic acid
provides
oligonucleotides
Prior art date
Application number
PCT/US2002/032088
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English (en)
Other versions
WO2003035829A2 (fr
Inventor
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Original Assignee
Nanosphere Inc
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/008,978 external-priority patent/US6984491B2/en
Application filed by Nanosphere Inc, So-Jung Park, Thomas A Taton, Chad A Mirkin, Robert L Letsinger, Robert C Mucic, James J Storhoff, Robert Elghanian, Viswanadham Garimella, Zhi Li, Gang Lu filed Critical Nanosphere Inc
Priority to CA2463323A priority Critical patent/CA2463323C/fr
Priority to AU2002363062A priority patent/AU2002363062B2/en
Priority to JP2003538330A priority patent/JP4347049B2/ja
Priority to EP02799155A priority patent/EP1478774A4/fr
Publication of WO2003035829A2 publication Critical patent/WO2003035829A2/fr
Publication of WO2003035829A3 publication Critical patent/WO2003035829A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés servant à détecter un acide nucléique. Ces procédés consistent à mettre en contact cet acide nucléique avec un ou plusieurs types de particules auxquelles sont fixés des oligonucléotides. Dans un mode de réalisation de ce procédé, les oligonucléotides sont liés à des nanoparticules et possèdent des séquences complémentaires de parties de la séquence de l'acide nucléique. L'hybridation des oligonucléotides sur les nanoparticules à l'acide nucléique provoque une modification pouvant être détectée (de préférence, une modification de couleur). Elle concerne également des compositions et des trousses contenant des particules. Elle concerne, de plus, des procédés de synthèse de conjugués uniques constitués par des nanoparticules et des oligonucléotides, les conjugués obtenus au moyen de ces procédés et des procédés de mise en application de ces conjugués. Elle concerne également des nanomatériaux et des nanostructures comprenant des nanoparticules, ainsi que des procédés de nanofabrication au moyen de ces nanoparticules. Elle concerne enfin un procédé de séparation d'un acide nucléique sélectionné d'autres acides nucléiques.
PCT/US2002/032088 2001-10-09 2002-10-08 Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules WO2003035829A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2463323A CA2463323C (fr) 2001-10-09 2002-10-08 Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules
AU2002363062A AU2002363062B2 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor
JP2003538330A JP4347049B2 (ja) 2001-10-09 2002-10-08 オリゴヌクレオチドを付着させたナノ粒子とその使用方法
EP02799155A EP1478774A4 (fr) 2001-10-09 2002-10-08 Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32786401P 2001-10-09 2001-10-09
US60/327,864 2001-10-09
US10/008,978 US6984491B2 (en) 1996-07-29 2001-12-07 Nanoparticles having oligonucleotides attached thereto and uses therefor
US10/008,978 2001-12-07

Publications (2)

Publication Number Publication Date
WO2003035829A2 WO2003035829A2 (fr) 2003-05-01
WO2003035829A3 true WO2003035829A3 (fr) 2004-08-26

Family

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Family Applications (1)

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PCT/US2002/032088 WO2003035829A2 (fr) 2001-10-09 2002-10-08 Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules

Country Status (5)

Country Link
EP (1) EP1478774A4 (fr)
JP (1) JP4347049B2 (fr)
AU (1) AU2002363062B2 (fr)
CA (1) CA2463323C (fr)
WO (1) WO2003035829A2 (fr)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

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EP3656735A1 (fr) * 2004-04-23 2020-05-27 Becton, Dickinson and Company Nanoparticules composites actives en spectroscopie exaltée de surface
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Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7259252B2 (en) 1996-07-29 2007-08-21 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US8323888B2 (en) 1996-07-29 2012-12-04 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6969761B2 (en) 1996-07-29 2005-11-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

Also Published As

Publication number Publication date
JP4347049B2 (ja) 2009-10-21
EP1478774A4 (fr) 2006-11-29
WO2003035829A2 (fr) 2003-05-01
AU2002363062B2 (en) 2007-03-22
CA2463323C (fr) 2010-12-21
EP1478774A2 (fr) 2004-11-24
CA2463323A1 (fr) 2003-05-01
JP2005525084A (ja) 2005-08-25

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