[go: up one dir, main page]

WO2006066001A3 - Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes - Google Patents

Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes Download PDF

Info

Publication number
WO2006066001A3
WO2006066001A3 PCT/US2005/045429 US2005045429W WO2006066001A3 WO 2006066001 A3 WO2006066001 A3 WO 2006066001A3 US 2005045429 W US2005045429 W US 2005045429W WO 2006066001 A3 WO2006066001 A3 WO 2006066001A3
Authority
WO
WIPO (PCT)
Prior art keywords
transfection
immobilized
culture device
eukaryotic cells
cationic polymer
Prior art date
Application number
PCT/US2005/045429
Other languages
English (en)
Other versions
WO2006066001A2 (fr
Inventor
Yasunobu Tanaka
Chris P Castello
Lei Yu
Original Assignee
Nitto Denko Corp
Yasunobu Tanaka
Chris P Castello
Lei Yu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nitto Denko Corp, Yasunobu Tanaka, Chris P Castello, Lei Yu filed Critical Nitto Denko Corp
Priority to AU2005316501A priority Critical patent/AU2005316501B2/en
Priority to JP2007546895A priority patent/JP4739352B2/ja
Priority to CA002590820A priority patent/CA2590820A1/fr
Priority to KR1020137000063A priority patent/KR20130010036A/ko
Priority to EP05854197A priority patent/EP1836293A2/fr
Publication of WO2006066001A2 publication Critical patent/WO2006066001A2/fr
Publication of WO2006066001A3 publication Critical patent/WO2006066001A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un dispositif de transfection/culture cellulaire qui comprend un support solide recouvert d'un cation polymère dégradable en tant que réactif de transfection. Ce dispositif de transfection/culture cellulaire est stocké de manière pratique à température ambiante jusqu'à son utilisation. Pour effectuer facilement la transfection cellulaire, l'acide nucléique d'intérêt et les cellules à transfecter sont ajoutés au dispositif de transfection/culture. La transfection cellulaire est achevée en moins d'une heure au moyen du dispositif de transfection/culture selon l'invention.
PCT/US2005/045429 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes WO2006066001A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2005316501A AU2005316501B2 (en) 2004-12-17 2005-12-14 Immobilized degradable cationic polymer for transfecting eukaryotic cells
JP2007546895A JP4739352B2 (ja) 2004-12-17 2005-12-14 真核細胞をトランスフェクトするための固定化分解性カチオンポリマーを持つ固体表面
CA002590820A CA2590820A1 (fr) 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes
KR1020137000063A KR20130010036A (ko) 2004-12-17 2005-12-14 진핵 세포의 트랜스펙션을 위한 고정화된 분해가능한 양이온성 중합체
EP05854197A EP1836293A2 (fr) 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63734404P 2004-12-17 2004-12-17
US60/637,344 2004-12-17

Publications (2)

Publication Number Publication Date
WO2006066001A2 WO2006066001A2 (fr) 2006-06-22
WO2006066001A3 true WO2006066001A3 (fr) 2006-09-08

Family

ID=36353906

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/045429 WO2006066001A2 (fr) 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes

Country Status (7)

Country Link
EP (1) EP1836293A2 (fr)
JP (1) JP4739352B2 (fr)
KR (2) KR20130010036A (fr)
CN (1) CN101124316A (fr)
AU (1) AU2005316501B2 (fr)
CA (1) CA2590820A1 (fr)
WO (1) WO2006066001A2 (fr)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US9028773B2 (en) 2001-09-12 2015-05-12 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US9051604B2 (en) 2001-02-14 2015-06-09 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9109229B2 (en) 2004-07-26 2015-08-18 Pfenex Inc. Process for improved protein expression by strain engineering
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
EP3718635A1 (fr) 2003-07-31 2020-10-07 Handylab, Inc. Traitement d'échantillons contenant des particules
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7358223B2 (en) 2004-10-04 2008-04-15 Nitto Denko Corporation Biodegradable cationic polymers
JP5415253B2 (ja) 2006-03-24 2014-02-12 ハンディラブ・インコーポレーテッド 微小流体サンプルを処理するための一体化システム及びその使用方法
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008060604A2 (fr) 2006-11-14 2008-05-22 Handylab, Inc. Système microfluidique utilisé pour amplifier et détecter des polynucléotides en parallèle
WO2008061165A2 (fr) 2006-11-14 2008-05-22 Handylab, Inc. Cartouche microfluidique et son procédé de fabrication
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
AU2012242510B2 (en) 2011-04-15 2015-05-14 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
EP2773892B1 (fr) 2011-11-04 2020-10-07 Handylab, Inc. Dispositif de préparation d'échantillons de polynucléotides
US10822644B2 (en) 2012-02-03 2020-11-03 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
CN102827756B (zh) * 2012-06-25 2014-04-09 华南农业大学 应用于大豆下胚轴外植体转化受体系统的农杆菌局部侵染方法和装置
BR112015032225B1 (pt) * 2013-06-28 2022-05-17 Ethris Gmbh Composição, composição farmacêutica, uso de uma composição, e, método in vitro para dispensação de rna de fita simples a uma célula alvo ou tecido
US12263229B2 (en) 2014-11-10 2025-04-01 Ethris Gmbh Induction of osteogenesis by delivering BMP encoding RNA
CN109983127B (zh) * 2016-10-05 2023-07-04 先正达参股股份有限公司 基因沉默的改善或与基因沉默有关的改善
CN109735573A (zh) * 2019-02-19 2019-05-10 武汉普诺赛生命科技有限公司 一种瞬时转染试剂及其应用方法
EP3798250A1 (fr) * 2019-09-25 2021-03-31 University College Dublin Polymères cationiques hyperramifiés utiles en tant que vecteurs d'administration d'acide nucléique pour la transfection
CN112195193A (zh) * 2020-09-30 2021-01-08 上海交通大学 单链dna在基因转染中的应用
CN114904003B (zh) * 2021-02-09 2023-09-29 广州立得生物医药科技有限公司 可电离的阳离子脂质类似物材料在作为核酸药物递送载体或转染试剂中的应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
EP1398042A1 (fr) * 2002-09-10 2004-03-17 Lipotek, Inc Transfection d' acides nucleiques
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
WO2005023312A2 (fr) * 2003-09-03 2005-03-17 Imperial College Innovations Limited Procedes
US20050176132A1 (en) * 2004-02-10 2005-08-11 Yasunobu Tanaka Culture device and method for eukaryotic cell transfection

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
DE60031997D1 (de) * 1999-09-17 2007-01-04 Whitehead Biomedical Inst Umkehrtransfektionsverfahren
ATE351902T1 (de) * 2000-11-03 2007-02-15 Univ Michigan Oberflächentransfektion und expressionsverfahren
WO2003106636A2 (fr) * 2002-06-14 2003-12-24 Mirus Corporation Nouveaux procedes d'apport de polynucleotides dans des cellules
US6878374B2 (en) * 2003-02-25 2005-04-12 Nitto Denko Corporation Biodegradable polyacetals
DE602004028664D1 (de) * 2004-02-18 2010-09-23 Nitto Denko Corp Kulturvorrichtung und verfahren zur transfektion eukaryontischer zellen

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
EP1398042A1 (fr) * 2002-09-10 2004-03-17 Lipotek, Inc Transfection d' acides nucleiques
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
WO2005023312A2 (fr) * 2003-09-03 2005-03-17 Imperial College Innovations Limited Procedes
US20050176132A1 (en) * 2004-02-10 2005-08-11 Yasunobu Tanaka Culture device and method for eukaryotic cell transfection

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9051604B2 (en) 2001-02-14 2015-06-09 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9028773B2 (en) 2001-09-12 2015-05-12 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US9458487B2 (en) 2002-10-08 2016-10-04 Pfenex, Inc. Expression of mammalian proteins in pseudomonas fluorescens
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US9109229B2 (en) 2004-07-26 2015-08-18 Pfenex Inc. Process for improved protein expression by strain engineering
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder

Also Published As

Publication number Publication date
CA2590820A1 (fr) 2006-06-22
AU2005316501B2 (en) 2010-10-21
JP2008523810A (ja) 2008-07-10
KR101263173B1 (ko) 2013-05-13
JP4739352B2 (ja) 2011-08-03
KR20070101859A (ko) 2007-10-17
WO2006066001A2 (fr) 2006-06-22
CN101124316A (zh) 2008-02-13
AU2005316501A1 (en) 2006-06-22
EP1836293A2 (fr) 2007-09-26
KR20130010036A (ko) 2013-01-24

Similar Documents

Publication Publication Date Title
WO2006066001A3 (fr) Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes
NZ593080A (en) Novel reagents for transfection of eukaryotic cells
WO2008092041A3 (fr) Biocapteurs optiques
WO2008118392A3 (fr) Plate-formes synthétiques de cellules et leurs procédés d'utilisation
WO2009085573A3 (fr) Dispositif cellulaire de contrôle qualité destiné à l'immunohistochimie et ses procédés d'utilisation
BRPI0514778A (pt) meio e cultivo das células tronco embriÈnica
BRPI0619794B8 (pt) Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
EP2410043A3 (fr) Systemes de culture de cellules souches
EP2169048A3 (fr) Système et procédé de culture de cellules
WO2007031871A3 (fr) Dispositif support pour culture cellulaire
IL214529A0 (en) Bioreactor for the cultivation of mammalian cells and methods of use thereof
WO2007084364A3 (fr) Il-12 améliorée aux fins d'expression dans des cellules mammaliennes
WO2008136398A1 (fr) Procédé de culture cellulaire utilisant un milieu enrichi en acide aminé
WO2006071466A8 (fr) Procedes et acides nucleiques pour l'analyse de l'expression genetique associee au pronostic de troubles proliferatifs de cellules prostatiques
WO2008108746A3 (fr) Milieu de culture cellulaire microfluidique
WO2012003479A3 (fr) Procédés de culture de cellules indifférenciées à l'aide de compositions à libération prolongée
WO2009096754A3 (fr) Dispositif de suivi de la course du soleil
DK1720979T3 (da) Anvendelse af et serumfrit celledyrkningsmedium til fremstilling af IL-18BP I pattedyrceller
WO2010130306A8 (fr) Système de bioréacteur
WO2007092222A3 (fr) Procede de caracterisation d'un compose biologiquement actif
WO2004003493A3 (fr) Methodes et trousses servant a determiner la depense energetique dans des organismes vivants par la production d'eau metabolique
ATE499598T1 (de) Markierungsfreier einzelzelltest
IL188396A0 (en) Natively glycosylated mammalian biological molecules prouced by eletromagnetically stimulating living mammalian cells
WO2007106149A3 (fr) Détection d'isotopes et leurs utilisations
WO2004113515A3 (fr) Modelisation de tissu dans un systeme de cellules souches embryonnaires (es)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LC LK LR LS LT LU LV LY MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2005316501

Country of ref document: AU

Ref document number: 2590820

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2007546895

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 200580043453.1

Country of ref document: CN

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2005316501

Country of ref document: AU

Date of ref document: 20051214

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 2005316501

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 1020077015797

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 2005854197

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2005854197

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 1020137000063

Country of ref document: KR