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WO2007010552A3 - Conjugue d'erythropoietine peg n-terminal - Google Patents

Conjugue d'erythropoietine peg n-terminal Download PDF

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Publication number
WO2007010552A3
WO2007010552A3 PCT/IN2006/000103 IN2006000103W WO2007010552A3 WO 2007010552 A3 WO2007010552 A3 WO 2007010552A3 IN 2006000103 W IN2006000103 W IN 2006000103W WO 2007010552 A3 WO2007010552 A3 WO 2007010552A3
Authority
WO
WIPO (PCT)
Prior art keywords
erythropoietin
formula
conjugate
epo
peg
Prior art date
Application number
PCT/IN2006/000103
Other languages
English (en)
Other versions
WO2007010552A2 (fr
WO2007010552B1 (fr
Inventor
Subhash V Kapre
Umesh S Shaligram
Kwang Nho
Gunwon Bae
Original Assignee
Serum Inst India Ltd
Sunbio Inc
Subhash V Kapre
Umesh S Shaligram
Kwang Nho
Gunwon Bae
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serum Inst India Ltd, Sunbio Inc, Subhash V Kapre, Umesh S Shaligram, Kwang Nho, Gunwon Bae filed Critical Serum Inst India Ltd
Publication of WO2007010552A2 publication Critical patent/WO2007010552A2/fr
Publication of WO2007010552A3 publication Critical patent/WO2007010552A3/fr
Publication of WO2007010552B1 publication Critical patent/WO2007010552B1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Epidemiology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un conjugué d'érythropoïétine à bioactivité supérieure comprenant une glycoprotéine érythropoïétine avec un groupe a - amino et des dérivés aldéhyde de polyéthylène glycol N-terminal, ladite glycoprotéine érythropoïétine étant sélectionnée dans le groupe formé de l'érythropoïétine humaine et de ses analogues, ledit dérivé aldéhyde de méthoxy polyéthylène glycol étant représenté par la formule PEG-R-CHO, dans laquelle R = un groupe alkyle représenté par la formule -(CH2)n dans laquelle n = 2 à 4. La formule du conjugué de la présente invention est PEG-R-CONH-EPO, formule dans laquelle R = un groupe alkyle représenté par la formule -(CH2)n dans laquelle n = 2 à 4. L'érythropoïétine de la présente invention est une érythropoïétine de recombinaison (rhEPO) produite dans des cellules animales. Ledit conjugué d'érythropoïétine est préparé par expression, fermentation, et purification de la protéine rhEPO dans des cellules animales de recombinaison, et par mise en réaction de l'EPO avec PEG-aldéhyde pour obtenir une EPO à PEG N-terminal par une réaction d'amination réductrice, celle-ci étant ensuite purifiée pour obtenir une EPO à PEG N-terminal pure.
PCT/IN2006/000103 2005-03-17 2006-03-16 Conjugue d'erythropoietine peg n-terminal WO2007010552A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN291MU2005 2005-03-17
IN291/MUM/2005 2005-03-17

Publications (3)

Publication Number Publication Date
WO2007010552A2 WO2007010552A2 (fr) 2007-01-25
WO2007010552A3 true WO2007010552A3 (fr) 2007-07-12
WO2007010552B1 WO2007010552B1 (fr) 2007-08-23

Family

ID=37528505

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2006/000103 WO2007010552A2 (fr) 2005-03-17 2006-03-16 Conjugue d'erythropoietine peg n-terminal

Country Status (1)

Country Link
WO (1) WO2007010552A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2594279B1 (fr) 2005-08-05 2017-11-22 Araim Pharmaceuticals, Inc. Peptides protecteurs de tissus et utilisations associées
JP5514736B2 (ja) 2008-01-11 2014-06-04 セリナ・セラピユーテイツクス・インコーポレーテツド ポリオキサゾリン共重合体の多機能形態およびそれを含む薬物組成物
US8101706B2 (en) 2008-01-11 2012-01-24 Serina Therapeutics, Inc. Multifunctional forms of polyoxazoline copolymers and drug compositions comprising the same
EP2245056B1 (fr) * 2008-01-22 2015-03-11 Araim Pharmaceuticals, Inc. Peptides protecteurs de tissu et analogues peptidiques pour la prévention et le traitement de maladies et de troubles associés à un endommagement tissulaire
SI2616101T1 (sl) 2010-09-14 2014-10-30 F. Hoffmann-La Roche Ag Postopek za čiščenje pegiliranega eritropoetina
CN102453087B (zh) * 2010-10-22 2013-12-25 广东赛保尔生物医药技术有限公司 一种单取代peg-epo的纯化及制备方法
CN102816227A (zh) * 2012-08-30 2012-12-12 深圳赛保尔生物药业有限公司 回收促红细胞生成素的方法
CN109096483B (zh) * 2017-06-28 2020-09-04 北京键凯科技股份有限公司 分枝型多元甘醇环氧衍生物交联透明质酸钠凝胶及其制备和应用
ES2905105T3 (es) 2017-12-29 2022-04-07 Hoffmann La Roche Procedimiento para proporcionar una composición de proteína PEGilada
KR102497097B1 (ko) * 2017-12-29 2023-02-06 에프. 호프만-라 로슈 아게 페길화 단백질 조성물의 제공 방법
SG11202005990RA (en) * 2017-12-29 2020-07-29 Hoffmann La Roche Process for providing pegylated protein composition
KR20210090649A (ko) * 2018-11-05 2021-07-20 브리스톨-마이어스 스큅 컴퍼니 Peg화 단백질의 정제 방법
CN119080909A (zh) * 2024-08-29 2024-12-06 深圳赛保尔生物药业有限公司 负载peg-epo及间充质干细胞的组合物、药物及其制备方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996011953A1 (fr) * 1994-10-12 1996-04-25 Amgen Inc. Compositions de proteines ayant subi une modification chimique a l'extremite n-terminale et procedes
WO1998005363A2 (fr) * 1996-08-02 1998-02-12 Ortho-Mcneil Pharmaceutical, Inc. Polypeptides possedant un polymere soluble dans l'eau a n-terminal lie par covalence simple
WO2000032772A2 (fr) * 1998-11-30 2000-06-08 Eli Lilly And Company Composes erythropoietiques
WO2002049673A2 (fr) * 2000-12-20 2002-06-27 F. Hoffmann-La Roche Ag Conjugues d'erythropoietine
WO2004009627A1 (fr) * 2002-07-19 2004-01-29 Cangene Corporation Composes erythropoietiques pegyles
US20040116649A1 (en) * 2002-09-09 2004-06-17 Antoni Kozlowski Water-soluble polymer alkanals

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996011953A1 (fr) * 1994-10-12 1996-04-25 Amgen Inc. Compositions de proteines ayant subi une modification chimique a l'extremite n-terminale et procedes
WO1998005363A2 (fr) * 1996-08-02 1998-02-12 Ortho-Mcneil Pharmaceutical, Inc. Polypeptides possedant un polymere soluble dans l'eau a n-terminal lie par covalence simple
WO2000032772A2 (fr) * 1998-11-30 2000-06-08 Eli Lilly And Company Composes erythropoietiques
WO2002049673A2 (fr) * 2000-12-20 2002-06-27 F. Hoffmann-La Roche Ag Conjugues d'erythropoietine
WO2004009627A1 (fr) * 2002-07-19 2004-01-29 Cangene Corporation Composes erythropoietiques pegyles
US20040116649A1 (en) * 2002-09-09 2004-06-17 Antoni Kozlowski Water-soluble polymer alkanals

Also Published As

Publication number Publication date
WO2007010552A2 (fr) 2007-01-25
WO2007010552B1 (fr) 2007-08-23

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