WO2007016665A3 - Single use fluorescent assays for determination of analytes - Google Patents
Single use fluorescent assays for determination of analytes Download PDFInfo
- Publication number
- WO2007016665A3 WO2007016665A3 PCT/US2006/030244 US2006030244W WO2007016665A3 WO 2007016665 A3 WO2007016665 A3 WO 2007016665A3 US 2006030244 W US2006030244 W US 2006030244W WO 2007016665 A3 WO2007016665 A3 WO 2007016665A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- analyte
- reagent
- fluorescence
- nanocrystals
- fluid sample
- Prior art date
Links
- 238000003556 assay Methods 0.000 title abstract 6
- 239000012491 analyte Substances 0.000 abstract 7
- 239000003153 chemical reaction reagent Substances 0.000 abstract 5
- 239000012530 fluid Substances 0.000 abstract 5
- 239000002159 nanocrystal Substances 0.000 abstract 4
- 238000010791 quenching Methods 0.000 abstract 3
- 230000000171 quenching effect Effects 0.000 abstract 2
- 239000013060 biological fluid Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000005284 excitation Effects 0.000 abstract 1
- 238000005286 illumination Methods 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7769—Measurement method of reaction-produced change in sensor
- G01N2021/7786—Fluorescence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Nanotechnology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Materials Engineering (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A single-use fluorescence-based assay system for determining the presence of at least one analyte in a fluid sample, especially a biological fluid sample, comprises a first reagent comprising fluorescent nanocrystals, and a second reagent comprising a quenching moiety effective under at least a predetermined condition to bind to the first reagent to quench the fluorescence of the nanocrystals. The predetermined condition may be the absence of the analyte in a fluid sample or, in alternative embodiments, the presence of the analyte in the fluid sample. Certain exemplary embodiments comprise a housing and a single-use assay component configured to be received by the housing and operative to receive sample fluid and perform an assay for at least one analyte suspected of being present in the sample fluid. The single-use fluorescence-based assay system optionally further comprises a fluorometer operative to generate excitation illumination at a detection site to cause fluorescence of the nanocrystals in the unquenched condition. In certain embodiments of the single-use fluorescence-based assay system a first reagent comprises fluorescent nanocrystals bearing one or more binding sites for the target analyte and a second reagent comprises the target analyte or an analog of the target analyte conjugated to quenching moieties.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70414305P | 2005-07-29 | 2005-07-29 | |
| US60/704,143 | 2005-07-29 | ||
| US70425205P | 2005-08-01 | 2005-08-01 | |
| US60/704,252 | 2005-08-01 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2007016665A2 WO2007016665A2 (en) | 2007-02-08 |
| WO2007016665A3 true WO2007016665A3 (en) | 2007-06-28 |
Family
ID=37709363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2006/030244 WO2007016665A2 (en) | 2005-07-29 | 2006-07-28 | Single use fluorescent assays for determination of analytes |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2007016665A2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5089705B2 (en) | 2008-11-20 | 2012-12-05 | アークレイ株式会社 | Optical measuring device |
| DE102014203266B4 (en) | 2014-02-24 | 2018-07-19 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Composition with FRET pair in defined geometry |
| EE01561U1 (en) | 2019-02-11 | 2022-03-15 | Qanikdx Oü | Method for detection and quantification of at least one organic analyte in a liquid sample. |
| CN111077001B (en) * | 2020-01-12 | 2022-05-10 | 天津市宝坻区人民医院 | Method for preparing anti-platelet antibody biological slice |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020004246A1 (en) * | 2000-02-07 | 2002-01-10 | Daniels Robert H. | Immunochromatographic methods for detecting an analyte in a sample which employ semiconductor nanocrystals as detectable labels |
-
2006
- 2006-07-28 WO PCT/US2006/030244 patent/WO2007016665A2/en active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020004246A1 (en) * | 2000-02-07 | 2002-01-10 | Daniels Robert H. | Immunochromatographic methods for detecting an analyte in a sample which employ semiconductor nanocrystals as detectable labels |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007016665A2 (en) | 2007-02-08 |
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|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
| NENP | Non-entry into the national phase |
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