WO2008030995A8 - Use of novel pepc to produce succinate - Google Patents
Use of novel pepc to produce succinate Download PDFInfo
- Publication number
- WO2008030995A8 WO2008030995A8 PCT/US2007/077806 US2007077806W WO2008030995A8 WO 2008030995 A8 WO2008030995 A8 WO 2008030995A8 US 2007077806 W US2007077806 W US 2007077806W WO 2008030995 A8 WO2008030995 A8 WO 2008030995A8
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- pepc
- novel
- succinate
- produce succinate
- engineered bacteria
- Prior art date
Links
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 title abstract 3
- 108091000041 Phosphoenolpyruvate Carboxylase Proteins 0.000 abstract 3
- 241000894006 Bacteria Species 0.000 abstract 2
- 241000192130 Leuconostoc mesenteroides Species 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- NONJJLVGHLVQQM-JHXYUMNGSA-N phenethicillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C(C)OC1=CC=CC=C1 NONJJLVGHLVQQM-JHXYUMNGSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A Leuconostoc mesenteroides phosphoenolpyruvate carboxylase enzyme has been identified that improves succinate production in engineered bacteria. Expression of the novel PEPC enzyme by engineered bacteria increases production and conversion of carbon to product is improved. Methods are described for making succinate using bacterial cultures expressing L. mesenteroides PEPC.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82471706P | 2006-09-06 | 2006-09-06 | |
| US60/824,717 | 2006-09-06 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2008030995A2 WO2008030995A2 (en) | 2008-03-13 |
| WO2008030995A3 WO2008030995A3 (en) | 2008-10-23 |
| WO2008030995A8 true WO2008030995A8 (en) | 2009-07-16 |
Family
ID=39158070
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2007/077806 WO2008030995A2 (en) | 2006-09-06 | 2007-09-06 | Use of novel pepc to produce succinate |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2008030995A2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7569380B2 (en) | 2004-12-22 | 2009-08-04 | Rice University | Simultaneous anaerobic production of isoamyl acetate and succinic acid |
| WO2011163128A1 (en) | 2010-06-21 | 2011-12-29 | William Marsh Rice University | Engineered bacteria produce succinate from sucrose |
-
2007
- 2007-09-06 WO PCT/US2007/077806 patent/WO2008030995A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008030995A2 (en) | 2008-03-13 |
| WO2008030995A3 (en) | 2008-10-23 |
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