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WO2008033009A1 - Utilisation effective de diélectrophorèse dans des microcanaux en serpentin - Google Patents

Utilisation effective de diélectrophorèse dans des microcanaux en serpentin Download PDF

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Publication number
WO2008033009A1
WO2008033009A1 PCT/NL2007/000217 NL2007000217W WO2008033009A1 WO 2008033009 A1 WO2008033009 A1 WO 2008033009A1 NL 2007000217 W NL2007000217 W NL 2007000217W WO 2008033009 A1 WO2008033009 A1 WO 2008033009A1
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WO
WIPO (PCT)
Prior art keywords
micro channel
particles
intermediate portion
dep
electrodes
Prior art date
Application number
PCT/NL2007/000217
Other languages
English (en)
Inventor
Adrianus Bossche
Florin Tatar
Lujun Zhang
Original Assignee
Stichting Voor De Technische Wetenschappen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stichting Voor De Technische Wetenschappen filed Critical Stichting Voor De Technische Wetenschappen
Publication of WO2008033009A1 publication Critical patent/WO2008033009A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength

Definitions

  • the present invention relates to the use of dielectrophoresis (DEP) for compound separation in curved micro-channels.
  • DEP dielectrophoresis
  • CE Capillary electrophoresis
  • CE is widely used in laboratory situations for off-line measurement, it has still not found its way to in-line applications in industrial processes, not even after the recent developments of CE microchips.
  • the drawbacks to be overcome for CE micro system integration are: the high separation voltages required (1500-3000V), fluidic interfacing between process and CE microchip, sample pre-treatment (filtering, enrichment, fluorescence labelling etc.).
  • the channel length required is too large for efficient micro-chip integration when using straight separation channels, since chip cost grows with chip area.
  • serpentine channels have been introduced. However, this is at the cost of a good separation quality.
  • a non-uniform electric field is generated by applying a voltage over a circular channel. Driven by the electro-osmotic flow, the particles with different dielectric properties move continuously to a different location across the channel as they flow due to the different DEP forces. At the end of the channel, different outlets are present through which particles having different properties are separated.
  • a disadvantage of the circular channel is that, in a single channel layer, only a single circle can be effectively used for separation. Additional circles can be added to form a spiral, however, because of the increasing diameters the effective contribution to the dielectrophoretic separation quickly diminishes.
  • a micro channel system comprising a micro channel, wherein the micro channel comprises at least first and second ends and at least two electrodes, one on either end of said micro channel, and at least a first and a second curved portion and a straight intermediate portion between the first and said second curved portion, wherein during operation, a sample mixture of particles can be driven along the micro channel by electro-osmotic flow by means of applying a voltage across said two electrodes, wherein due to a difference in DEP force, said particles with different DEP responses move to different locations across the first and second curved portions as they flow, wherein in the straight intermediate portion positioning means are provided arranged to position, during operation, particles having different size or different DEP-sign at substantially the same position in front of the second curved portion.
  • the positioning means comprise electrodes arranged on a inner wall of said intermediate portion.
  • the electrodes may for example be triangular shaped. This will produce a suitable electric field for positioning the particles.
  • the positioning means comprise topographic arranged inside the intermediate portion. These topographic static structures are nonelectrical and can be produced together with the micro-channel fabrication. In an embodiment, the topographic structures are protruding from one or more walls of the channel.
  • the topographic structures may comprise an array of posts arranged between a top wall and a bottom wall of said intermediate portion.
  • the topographic structures comprise a plurality of parallel hurdles arranged on a bottom wall of said intermediate portion.
  • a main direction of each of said plurality of parallel hurdles forms an angle with a side wall of said intermediate portion, said angle being unequal to 90 DEG.
  • FIG. 3 illustrates an example of dielectrophoretic separation of particles in a curved channel
  • - Figure 4 shows a serpentine channel according to an embodiment in which negative DEP particles having different sizes are separated;
  • - Figure 5 shows an intermediate portion comprising squared posts;
  • Figure 6 shows a cross sectional view of the intermediate portion of Figure 5;
  • Figure 8 shows a cross sectional view of the channel of Figure 7;
  • FIG. 9 shows a three dimensional view of an embodiment of the intermediate portion having parallel hurdles
  • Figure 10 shows a top view of the embodiment of FigurelO;
  • This invention uses non-uniform electric fields in channel curves for dielectrophoretic (DEP) separation of particles.
  • Dielectrophoresis is the movement of particles induced by polarization effects in non-uniform electric fields.
  • the dielectrophoretic force acting on a spherical particle can be described by:
  • V£ 2 is the gradient of electric field squared
  • ⁇ - is the permittivity of the suspending medium
  • r is the radius of the particle
  • K ⁇ ) is the frequency dependent Claussius-Mosotti (CM) factor
  • ⁇ " and ⁇ m ' represent the frequency dependent complex permittivities of the particle and medium, respectively
  • Positive DEP occurs whenicT(c ⁇ ) > 0 , the force is toward the high electric field.
  • the converse of this is negative DEP, which occurs wheniT( ⁇ ) ⁇ 0 , the force is in the direction of decreasing field intensity.
  • Figures 1 and 2 show the principle of DEP force. The direction of the particle movement depends on the specific DEP response. Figure 1 shows that positive DEP particles move in the direction of increasing E-field and Figure 2 shows that negative
  • Figure 3 illustrates an example of dielectrophoretic separation of particles in a curved channel 10.
  • a non-uniform electric field will be generated whose gradient directs towards a center 13 of the curved channel 10.
  • the curved channel comprises an inlet 14 through which a sample mixture is applied.
  • the sample mixture is driven along the curved channel 10 by the electro-osmotic flow. Due to the different DEP force magnitudes and directions, particles with different DEP responses move continuously to the different location across the curved channel 10 as they flow. For example, particles 16 with a positive DEP feature will follow a trajectory like trajectory 17 and particles 18 with a negative DEP feature will follow a trajectory like trajectory 19. Since the flow at the inner side is faster than that at the outer side of the channel, the particle running at the outer side path will be left behind.
  • FIG. 4 shows an embodiment of the invention in which the above described separation mechanism is used to separate negative DEP particles having different sizes.
  • a serpentine shaped separation channel 20 comprises at least two curved portions 21, 22 (also referred to as curves 21, 22) and a straight intermediate portion 23.
  • Fluid such as water, enters the channel 20 by way of an inlet 24 and leaves the channel 20 through an outlet 25.
  • a voltage is applied by means of two electrodes 26, 27 so that a sample mixture is driven along the channel by the electro-osmotic flow.
  • the sample mixture is applied in the channel 20 through a sample inlet 28.
  • the sample mixture comprises negative DEP particles having different sizes.
  • the straight intermediate portion 23 comprises positioning means used to compel all the particles to the same point of assembly 30 before the next curve 22, see Figure 4.
  • the point of assembly is a point close to an inner wall of the next curve 22.
  • the positioning means may comprise non-electrical topographic structures causing non-uniform electrical fields that drive all particles to one side of the channel 20 so that all particles will enter the next curve 22 on the inside.
  • Such topographic structures for particle concentration have been described in literature [1, 2]. However, the topographic structure were not used in conjunction with serpentine micro channels used for dielectrophoretic separation.
  • a portion 44 of the micro channel 20 upstream the first curved portion 21 also comprises positioning means for bringing specific particles to an assembly point, e.g. the inner side of the micro channel 20, see Figure 4.
  • the straight intermediate portion 23 comprises squared posts 50 standing from bottom to top in the channel 20.
  • the DEP force between the posts 50 build up a barrier by which the particles are forced to go through narrow channels, see dotted lines, and end up at the other side of the channel 20.
  • This embodiment is only for positive DEP particles, since a barrier between the rows of posts 70 are formed by a low electric field region. The positive DEP particles will be blocked from passing through the low field regions and can only follow the small channels between the columns of the posts.
  • Figure 6 shows a cross sectional view of the straight intermediate portion 23 across the line VI-VI in Figure 5.
  • Figure 7 shows an embodiment wherein rectangular posts 70 are utilized to repel the particles to the other side of the channel 20.
  • Figure 8 shows a cross sectional view of the channel of Figure 7.
  • Figure 9 shows a three dimensional view of yet another embodiment of a straight intermediate portion 23 of the micro channel 20 wherein the topographic structures comprise parallel hurdles 90 standing on a bottom wall but that do not touch a top wall of the channel 20.
  • This embodiment can be used for both positive DEP and negative DEP particles.
  • Figure 10 shows a top view of the embodiment of FigurelO. From Figure 10, one can see that the particles all flow to one side of the micro channel 20.
  • the straight intermediate portion 23 comprises electrodes on both top and bottom side of the micro channel 20 to bring particles from one side to the other side of the straight portion of the channel.
  • Figure 11 shows a simulation of particle trajectories in a serpentine channel using Matlab. Due to an electric field produced between top and bottom electrodes, particles will follow the trajectories shown in Figure 11. As can be seen from Figure 11, the particles all start at the same position before going in the third curved part 110 of the channel 20.
  • a plurality of S-shaped channels are cascaded together to yield higher separation resolution. After a number of curves, the smaller negative DEP particles leave behind the larger negative DEP particles thereby separated.
  • topographic dielectrophoretic structures can also be used for sample filtering and concentration in the sample injection channel. When the topographic structures are placed at the sample inlet port, only the target particles could enter the separation channel while the larger particles are blocked outside due to the larger DEP forces. When the topographic structures are placed at the sample outlet port, the target particles will stay in the separation channel while the smaller particles are filtered out.

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  • Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Electrostatic Separation (AREA)

Abstract

La présente invention concerne un système de microcanal formé d'un microcanal comprenant au moins une première et une seconde extrémités, au moins une première et une seconde parties incurvées ainsi qu'une partie droite intermédiaire située entre la première et la seconde parties incurvées. Dans la partie droite intermédiaire se trouvent des points d'ancrage destinés à retenir, au cours du fonctionnement, des particules de taille et de signe DEP différents sensiblement dans la même position face à la seconde partie incurvée. En positionnant les particules face à la partie incurvée suivante, on a la possibilité d'exploiter le maximum des forces de diélectrophorèse pour la séparation dans un microcanal en serpentin.
PCT/NL2007/000217 2006-09-14 2007-09-06 Utilisation effective de diélectrophorèse dans des microcanaux en serpentin WO2008033009A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06120653.8 2006-09-14
EP06120653 2006-09-14

Publications (1)

Publication Number Publication Date
WO2008033009A1 true WO2008033009A1 (fr) 2008-03-20

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107974400A (zh) * 2017-11-21 2018-05-01 华南理工大学 一种耦合介电泳和空间分离的微流控细胞分选芯片及方法
WO2018140775A1 (fr) * 2017-01-26 2018-08-02 Delee Corp. Tri d'objets à l'aide d'une séparation par diélectrophorèse
CN115074240A (zh) * 2022-06-15 2022-09-20 大连海事大学 一种基于可变形微液滴的介电泳微颗粒多级分选装置及方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002088321A2 (fr) * 2001-05-02 2002-11-07 Applera Corporation Concentration et purification d'analytes au moyen de champs electriques
US20020182627A1 (en) * 2001-03-24 2002-12-05 Xiaobo Wang Biochips including ion transport detecting strucutres and methods of use
EP1614477A1 (fr) * 1999-09-30 2006-01-11 Wako Pure Chemical Industries Ltd Méthode de séparation de substances utilisant des forces diélectrophorétiques

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1614477A1 (fr) * 1999-09-30 2006-01-11 Wako Pure Chemical Industries Ltd Méthode de séparation de substances utilisant des forces diélectrophorétiques
US20020182627A1 (en) * 2001-03-24 2002-12-05 Xiaobo Wang Biochips including ion transport detecting strucutres and methods of use
WO2002088321A2 (fr) * 2001-05-02 2002-11-07 Applera Corporation Concentration et purification d'analytes au moyen de champs electriques

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
L. ZHANG: "Continuous electrodeless dielectrophoretic separation in a circular channel", JOURNAL OF PHYSICS / CONFERENCE SERIES 34 (2006), no. 34, April 2006 (2006-04-01), International MEMS conference 9-12 may 2006 Singapore, pages 527 - 532, XP002419510 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018140775A1 (fr) * 2017-01-26 2018-08-02 Delee Corp. Tri d'objets à l'aide d'une séparation par diélectrophorèse
CN107974400A (zh) * 2017-11-21 2018-05-01 华南理工大学 一种耦合介电泳和空间分离的微流控细胞分选芯片及方法
CN115074240A (zh) * 2022-06-15 2022-09-20 大连海事大学 一种基于可变形微液滴的介电泳微颗粒多级分选装置及方法

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