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WO2008034118A2 - Procédés et dispositifs destinés à des mesures utilisant une spectroscopie pompe-sonde dans des microcavités de haute qualité - Google Patents

Procédés et dispositifs destinés à des mesures utilisant une spectroscopie pompe-sonde dans des microcavités de haute qualité Download PDF

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WO2008034118A2
WO2008034118A2 PCT/US2007/078586 US2007078586W WO2008034118A2 WO 2008034118 A2 WO2008034118 A2 WO 2008034118A2 US 2007078586 W US2007078586 W US 2007078586W WO 2008034118 A2 WO2008034118 A2 WO 2008034118A2
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pump
resonant
changes
probe
sample
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WO2008034118A3 (fr
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Frank Vollmer
Juraj Topolancik
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President And Fellows Of Harvard College
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Publication of WO2008034118A3 publication Critical patent/WO2008034118A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7789—Cavity or resonator
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/26—Optical coupling means
    • G02B6/28—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals
    • G02B6/293—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means
    • G02B6/29331—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means operating by evanescent wave coupling
    • G02B6/29335—Evanescent coupling to a resonator cavity, i.e. between a waveguide mode and a resonant mode of the cavity
    • G02B6/29338—Loop resonators
    • G02B6/29343—Cascade of loop resonators

Definitions

  • the present invention comprises devices and methods that use optical microcavities as a spectroscopic tool for detecting molecules and for probing conformations and orientations of molecules and molecular assemblies.
  • microcavities In biosensing, the sensitivity of microcavities surpasses that of surface- plasmon resonance (SPR) which is widely recognized as the state-of-the-art label-free detection technique. Binding of only a few molecules on the microcavity surface shifts the frequencies of the resonant modes that evanescently interact with the adsorbed material. Monitoring of the shift induced by polarizability or refractive index changes forms the basis of label-free, non-invasive, real-time biodetection and nanolayer characterization. The present invention extends this sensitive technique using devices and methods for pump-probe spectroscopy in high-Q microcavities which can be used to detect molecules and to track dynamic changes in the molecular structure.
  • SPR surface- plasmon resonance
  • Coupled plasmon-waveguide resonance (CPWR) spectroscopy has been developed to probe anisotropies in biological membranes immobilized onto solid surfaces by incorporating a TM- (transverse magnetic) polarized probe in addition to the TE- (transverse electric) polarized one used in conventional SPR.
  • TM- transverse magnetic
  • TE- transverse electric
  • High-Q optical microcavities represent an interesting alternative to SPR-based techniques providing exceptional sensitivity and two possible probing polarizations and operation at arbitrary wavelengths.
  • ⁇ polarizability changes
  • the microresonators represent a new quantitative tool for probing molecular transformations in important proteolipid biomolecular assemblies such as G- protein-coupled receptors or in signaling molecules such as calmodulin and disease- related prion proteins.
  • the present invention is particularly suited for pump-probe studies of photosensitive biomolecules.
  • thermo-optic effect has been demonstrated in toroidal microcavities utilizing only one beam at resonance with a microcavity (A. M. Armani*, R. P. Kulkarni, S. E. Fraser, R. C. Flagan, K. J. Vahala "Label-free, single-molecule detection with optical microcavities" Science,
  • the present invention utilizes a pump probe approach where the resonance wavelength of the pump beam can be chosen deliberately to maximize absorption by molecular analytes and particles. Although high circulating power is advantageous, the resonance excited by the pump beam does not have to be associated with a high-Q factor, since sensitive detection is achieved with a probe beam that (simultaneously or sequentially) excites a high-Q resonance in the same microcavity.
  • the resonance wavelength of the probe beam is chosen to maximize Q-factor and thus sensitivity for detection.
  • the present invention comprises devices and methods that use optical microcavities as a spectroscopic tool for detecting molecules as well as probing conformations and orientations of molecules and molecular assemblies.
  • the approach is based on the use of a pump beam and a probe beam which both excite resonance in the same optical microcavity where coupling can be achieved through one or more waveguides (Fig. 1).
  • Different light sources e.g. tunable lasers
  • Intensity and polarization for each wavelength can be controlled independently using combinations of in-line polarizers, couplers, and waveplates.
  • Transmission of the microcavity-waveguide system is measured using a photodetector coupled to a waveguide, or a photodetector is used to collect the light scattered from the microcavity
  • the pump beam interferes with molecules adsorbed on the microcavity and can e.g. be used to trigger conformational changes, break/form chemical bonds, induce excited states, excite vibrational and rotational molecular modes, induce/prevent chemical reactions or generate heat by absorption.
  • a probe beam non-invasively (meaning without triggering a similar response in the adsorbed molecules) measures such perturbations. This is possible since a change in molecular conformation, breakage/reformation of chemical bonds, polarizability, heat generation etc.
  • the resonance condition for the probe beam will change the resonance condition for the probe beam and thus the transfer characteristics of the waveguide-coupled microcavity system.
  • This can be measured for example by tracking a resonance in a transmission spectrum obtained by scanning a coherent source (probe).
  • a photodetector coupled to the output waveguide or using a photodetector that collects the light scattered from the microcavity (Fig. 1).
  • transverse electric (TE) and -magnetic (TM) polarized resonant modes extends sensing capabilities of microcavities by allowing anisotropy measurements, measurement of molecular orientation, and measurement of the orientation of the transition dipole moment (Fig. 2).
  • proteolipid membranes e.g. Bacteriorhodopsin, Fig.
  • the orientation of a transition dipole moment can be determined by exciting the molecule using the pump beam at different polarization (e.g. TE or TM).
  • the orientation of a molecular assembly can be determined by probing the perturbed (pump) or unperturbed molecule using the probe at different polarization (TE or TM).
  • the present invention is also based on operation of microcavities at arbitrary, e.g. visible and near-infrared (near-IR), wavelengths which is particularly important for noninvasive sensing with photosynthetic- or bacterial membranes that contain light-absorbing chromophores.
  • arbitrary wavelengths e.g. visible and near-infrared (near-IR)
  • the operation at arbitrary wavelengths is particularly important for noninvasive sensing because an optical probe could trigger molecular transitions in binding macromolecules, which would interfere with the analysis. This is the case for example with photosynthetic pigments (see S. G. Boxer, Stark Spectroscopy of Photosynthetic Systems (Academic Publishers, The Netherlands, 1996) or Rhodopsins (see R. R. Birge, Annu. Rev. Phys. Chem.
  • bR is ideal for such a demonstration since its structure is well known, it self-assembles easily onto a silica surface (see J.-A. He, L. Samuelson, L. Li, J. Kumar, S. K. Tripathy, Adv. Mater. 11, 435 (1999); J.-A. He, L. Samuelson, L. Li, J. Kumar, S. K. Tripathy, Langmuir 14, 1674 (1998)) and its molecular conformation can be switched optically between two stable states (see B. L. Feringa, Ed., Molecular Switches (Wiley- VCH GmbH, Weinheim, Germany, 2001) ).
  • the present invention is an optical device that comprises a high-Q resonant or slow light structure and one of an interface, a surface or a mode volume accessible for interactions with a sample.
  • Resonant or waveguide modes are excited in said high-Q or slow light structure at none, one or more pump wavelengths and at one or more probe wavelengths, and the resonant modes are excited simultaneously or sequentially.
  • the high Q structure may comprise at least one selected from the group of: microspherical cavities, microtoroidal cavities, microring-cavities, microdisk cavities, photonic crystal defect cavities, disordered photonic crystal waveguides, fabry-perot cavities, photonic crystal waveguides, donor-type photonic crystal cavities, acceptor-type photonic crystal cavities.
  • the resonant or waveguide modes may be linearly (e.g. transverse electric or transverse magnetic) or circularly polarized. Further, the resonant modes are excited by one of evanescent coupling from an optical waveguide, evanescent coupling from a photonic crystal waveguide, evanescent coupling from a tapered optical fiber, and evanescent coupling from a tapered optical fiber tip. Further, the resonant modes or waveguide modes may be excited using at least one selected from the group of: distributed feedback laser, an external cavity laser, an organic dye laser, surface emitting laser diodes, quantum dot emitters.
  • a wavelength (frequency) of a resonant mode is detected from spectral features of a transmission spectra recorded with photodiodes in a spectral regions. Transfer characteristics of the high Q structure may be determined from intensity measurements of transmitted or scattered light recorded with photodiodes in one ore more spectral regions.
  • a photodiode may be connected to a waveguide that connects to the high- Q or slow light structure. The photodiode collects scattered light from the high-Q or slow light structure.
  • the resonant mode is excited at two or more wavelengths simultaneously or sequentially.
  • the sample may be delivered to the interface, surface or mode volume using pressure operated microfluidic channels or microfluidic channels operated by electrophoresis or dieelectrophoresis.
  • the sample may self assemble on the said interface or surface.
  • the sample may comprise one of a liquid, a gas, a molecular monolayer, a self- assembled monolayer, an ultra-thin film, un-specifically adsorbed protein molecules, un- specif ⁇ cally adsorbed DNA molecules, specifically bound protein molecules, specifically bound DNA molecules, small organic molecules, antibodies, single molecule.
  • the sample may comprise one of an optically anisotropic component, a biological membrane such as photosynthetic membranes or rhodopsin containing bacterial membranes, a photochromic protein such as photoactive yellow protein or green/yellow fluorescent protein, a prion proteins, a signaling molecule such as calmodulin, a protein with one of photoactive yellow protein, green fluorescent protein or yellow fluorescent protein as a component, one or more photochromic compounds.
  • Figure 1 shows the schematic of an experimental set-up in accordance with a preferred embodiment of the present invention.
  • Fig 2 illustrates how linearly polarized microcavity resonances excite and probe the molecule/particle in the direction orthogonal to (TM) or parallel with (TE) respect to the microcavity surface (xy-plane) in accordance with a preferred embodiment of the present invention.
  • Fig. 3 illustrates a 3D ribbon-model of seven transmembrane ⁇ -helixes (white) representing the structure of bR protein embedded in a lipid bilayer (not shown) with a covalently bound all-trans retinal chromophore (black).
  • Fig. 4 illustrates an exemplary optical measurement set-up in accordance with a preferred embodiment of the present invention.
  • P 1 , P 2 polarizers; ⁇ /2, half-waveplate; PDi, PD 2 , near-IR photodiodes; PC, personal computer for data acquisition and analysis.
  • Fig. 4 illustrates an exemplary optical measurement set-up in accordance with a preferred embodiment of the present invention.
  • P 1 , P 2 polarizers; ⁇ /2, half-waveplate; PDi, PD 2 , near-IR photodiodes; PC, personal computer for data acquisition and analysis.
  • Fig. 1 , P 2 polarizers; ⁇ /2, half-waveplate;
  • Fig. 5 is a time trace of a TM -polarized resonance wavelength shift due to bR adsorption from a liquid solution that surrounds the microcavity.
  • the measured resonant shift at saturation, ⁇ 67pm, points to self-assembly of a bR monolayer.
  • Fig.6 is a transmission spectra for a TE and a TM polarized WGM.
  • a negative resonance wavelength shift is observed upon photoexcitation of the adsorbed bR monolayer. The shift is about twice as large for the TE than for the TM mode, which is due to the retinal orientation in the bR membrane.
  • Fig. 6 is a transmission spectra for a TE and a TM polarized WGM.
  • a negative resonance wavelength shift is observed upon photoexcitation of the adsorbed bR monolayer.
  • the shift is about twice as large for the TE than for the TM mode, which is due to the retinal orientation in the bR membrane.
  • Fig. 7 illustrates dynamics of the ground to M state transformation observed by tracking wavelength shifts of TE- and TM-polarized microcavity modes.
  • Fig. 8 illustrates angles defining the average orientation of the retinal relative to the polarization direction of the probing resonances.
  • Fig. 9 is a schematic diagram of the measurement setup used in one preferred embodiment of the present invention.
  • Vertically scattered light from a photonic crystal slab (PhC) is collected with an objective (O) and imaged with a lens (Ll) onto a field- stop (FS) consisting of a variable aperture which restricts analysis of scattered light to a certain area on the PhC slab.
  • Another lens (L2) re-focuses light from the selected area into an IR photodiode (PD).
  • a beam splitter redirects a fraction of the collimated beam into an IR charge coupled device camera (IR-CCD) for imaging.
  • IR-CCD IR charge coupled device camera
  • Inset shows example of a (scattering) spectrum for a high-Q cavity.
  • a tapered fiber tip is used to couple light to the photonic crystal structure.
  • optical microcavities are used as a sensitive spectroscopic tool for detecting molecules and probing conformations and orientations of molecular assemblies.
  • An exemplary setup and exemplary methods are described for measurements of photoinduced conformational changes in bacteriorhodopsin membrane.
  • An exemplary optical setup is described with reference to Fig. 4.
  • a sample cell 460 was constructed by threading a single mode optical fiber (SMF-28) 442 through a 1 cm polystyrene cuvette 462.
  • the fiber with a stripped polymer sheath was glued to the sides of the container and the exposed silica was etched with a 50% hydrofluoric (HF) acid. After a ⁇ 30-minute etch a taper with a diameter of ⁇ 2-3 ⁇ m was formed, as observed with an upright microscope (not shown) equipped with a long working distance objective. The taper was used to excite WGMs in optical resonators by evanescent field coupling.
  • a silica microsphere (-300 ⁇ m diameter) 222 on-a-stem 224 was prepared by melting the tip of a SMF-28 fiber in a butane -N 2 O flame. It was then mounted on a mechanical stage and brought on contact with the taper 226 as shown in Fig.
  • a tunable distributed- feedback (DFB) laser diode (probe) 402 operating around nm was coupled into the tapered fiber 226 via a fiber coupler 450.
  • a photodiode (PDi) 480 at the other end of the fiber recorded the transmission.
  • WGMs were identified as Lorentzian-shaped troughs in a spectrum obtained by periodically tuning the DFB laser current (/) at 100 Hz with a waveform generator.
  • the laser tuning coefficient ⁇ /l/ ⁇ J-0.0055 nm/mA was determined with a wavemeter.
  • Spectra containing 1,000 points per scan were recorded every -200 ms and the positions of the resonances were tracked with a computer using a LABVIEW routine based on polynomial fitting algorithm.
  • the width (at half-maximum) of these troughs reflects the intrinsic Q-factor and absorbance of the material surrounding the cavity.
  • a polarizer (Pi) 410 and a rotating half-waveplate 420 were used to selectively excite either TE or TM resonances.
  • the polarization direction of the monitored mode was verified, using another polarizer (P 2 ) 474 and a photodiode (PD 2 ) 476, by analyzing the light scattered tangentially off the microsphere. This arrangement was used to optimize the mode coupling efficiency into to resonator by slowly etching the microsphere and the fiber with a highly diluted HF solution ( ⁇ 1%). The process was terminated when the probe was critically coupled into the cavity, i.e.
  • bR forms a 5nm-thick membrane with a well defined surface density of monomers.
  • Each monomer contains a retinal chromophore surrounded by a seven- ⁇ -helix trans-membrane protein which is embedded in a lipid bilayer composed often Haloarchaeal lipids per bR monomer (Fig. 3).
  • the retinal is covalently bound to Lys216 residue via a protonated Schiff base (SB) at a fixed angle relative to the proteolipid membrane.
  • SB protonated Schiff base
  • bR thin-film formation on solid surfaces has been widely studied in the context of fabrication of optoelectronic devices and various techniques for controlled immobilization of oriented monolayers have been demonstrated.
  • electrostatic deposition of bR onto a PDAC- coated silica microsphere which was optimized for a monolayer formation.
  • the microsphere was sonicated for 15 minutes in 350 mM solution of KOH after which a positively charged polymer poly(dimethyldiallyl)ammoniumchloride (PDAC) was pre- adsorbed onto its surface to promote successive binding of the negatively charged bR membranes. This was done by exposing the sphere to a 2% PDAC solution in 0.5 M NaCl at a pH of 6.8 for 2 minutes.
  • PDAC poly(dimethyldiallyl)ammoniumchloride
  • the sphere was then rinsed in distilled water for additional 2 min.
  • the bR layer was formed in situ using 1 mg/ml sonicated stock solution of D96N bR mutant (Munich Innovative Biosystems) in Milli-Q water which was further diluted (molar ratio 1 :10) upon injection into the sample cell filled with PBS.
  • Monolayer formation [0026] The bR adsorption process was monitored by tracking the resonant wavelength shift of a TM mode. The time-trace of the shift shows Langmuir-like adsorption kinetics which points to self-assembly of a single layer at saturation (Fig. 5).
  • the monolayer formation is further confirmed by estimating the surface density of bR monomers, ⁇ tn, in the adsorbed layer which can be extracted from the resonant shift: where ⁇ / ⁇ is the fractional resonant wavelength shift at saturation, R is the microsphere radius, a is the molecular polarizability in excess to that of the displaced water, and n s and n m are the refractive indices of the sphere (1.47) and the PBS solution (1.33), respectively. (See S. Arnold, M. Khoshsima, I. Teraoka, S. Holler, F. Vollmer, Opt. Lett. 28, 272 (2003).).
  • the state of the bR is controlled with a low power ( ⁇ 200 ⁇ W), continuous wave, green pump laser coupled to the microsphere cavity using the tapered fiber (Fig. 2, Fig. 4).
  • the visible pump evanescently excites WGMs propagating around the microsphere's equator inducing the ground to M state conversion along their path (Fig. 2, Fig. 3).
  • a near-IR beam 1,310 nm excites the probing resonances.
  • the approximate bulk index change upon isomerization at the probing wavelength is only ⁇ -8x10 4 , as extrapolated from the Kramers-Kronig transformation of the published absorption data .
  • bR forms an anisotropic, ultra-thin macromolecular monolayer on the cavity surface where the evanescent field intensity is the highest. Although only a small fraction of the evanescent tail interacts with the bR membranes, we are sensitively probing molecular changes in a highly oriented anisotropic system. We therefore choose polarizability rather than refractive index to describe the photochromic transitions. Given the measured surface density of bR monomers, Eq. 1 can be used to calculate the average polarizability change at ⁇ pro b e of a single bR molecule upon photoexcitation:
  • Time-resolved switching data for a step modulation of a low-intensity, continuous wave pump is presented in Fig. 7. Since the intensity of the pump is resonantly enhanced by recirculation in the microcavity, moderate pump powers ( ⁇ 200 ⁇ W) are sufficient to fully photoexcite bR molecules.
  • the time traces of near-IR resonant wavelength positions for two polarizations reveal a fast timescale associated with the photoinduced M state buildup and a slow thermal relaxation into the ground state at r ⁇ l Is. The results are consistent with the timescales of photochromic transformations of D96N.
  • silica microspheres should be able to dynamically resolve nanosecond transitions and quantify molecular polarizabilities of the corresponding intermediaries.
  • E 0 I ⁇ s E(f) dV is the total electromagnetic mode energy contained in the interior of
  • microsphere with permittivity ⁇ s the microsphere with permittivity ⁇ s .
  • E TE « E TM ; ( ⁇ ) is related to the wavelength shift ratio of TE and TM modes upon retinal isomerization by: ⁇ TE . , I f 1 + cos 2 (g) + ⁇ 2 ⁇ ) ⁇ A ⁇ TM ⁇ 2 ⁇ sin 2 ( ⁇ ) + ⁇ cos 2 ( ⁇ ) J '
  • ⁇ Sa 11 / ⁇ a L .
  • the results for the retinal orientation are relatively insensitive to the precision of /lvalues used in the calculation.
  • the measured value of A ⁇ TE /A ⁇ TM corresponds to ( ⁇ ) of -61°. It is interesting to compare this result to measured retinal orientations obtained with alternative methods.
  • Sensitivity estimate [0035] We would like to comment on the sensitivity of our technique and its applicability to measurements of the polarizability and its anisotropies in molecular systems, particularly those that absorb visible light.
  • a typical silica microsphere (Q-IO 6 ) coated with biomolecules at a surface density of the order of 10 13 cm “2 can resolve the polarizability change (Aa of -3 au (-0.4 A 3 ) in the near-IR.
  • the rough estimate assumes an experimental resolution for the resonant shift of -1/50 of the linewidth.
  • the sensitivity is rather remarkable considering that the polarizability of a hydrogen molecule is -0.8 A 3 and trans-cis isomerization of a single C-C bond and deprotonation can change the polarizability by hundreds of atomic units.
  • conformational changes in such systems have been observed qualitatively by CPWR and indirectly by Stark spectroscopy, the magnitude of ⁇ that accompanies structural transformations in complex proteolipid macromolecules is not known.
  • the microresonators represent a new quantitative tool for probing molecular transformations in important proteolipid biomolecular assemblies such as G-protein- coupled receptors or in signaling molecules such as calmodulin and disease-related prion proteins.
  • the introduced technique is particularly suited for off-resonant pump-probe studies of photosensitive biomolecules.
  • Further examples of such systems include photosynthetic membranes and photoreceptors such as photoactive yellow protein.
  • the latter can also be used as photochromic labels in fusion proteins to study molecular processes and anisotropies imposed by their e.g. proteolipid environment.
  • further improvements of the microresonator technique promise single-molecule sensitivity which is beyond the scope of any alternative label-free technique.
  • High-refractive-index slabs with 2D arrays of air-holes exhibit large PBGs for the TE-like polarization (Fig. 9) (E-f ⁇ eld parallel to 2D-plane) (T. Krauss, R. DeLaRue, and S. Brand, Nature 383, 699 (1996)), which established them as a popular platform for designing waveguides (M. Loncar et al, J. Lightwave Technol. 18, 1402, (2000), S. G. Johnson et al, Phys. Rev. B, 62, 8212 (2000),M. Notomi et al, Phys. Rev. Lett. 87, 253902 (2001)), and nanocavities (O. Painter, J.
  • Fig. 9 Coherent light 960 from an infrared (IR) diode laser 940 tunable from 1,475 to 1,580 nm, was coupled into waveguide 970 (WIs) from a single-mode optical fiber (SMF-28).
  • WIs waveguide 970
  • SMF-28 single-mode optical fiber
  • PhC modes were excited with a non-linear fiber taper 930.
  • the taper prepared by pulling a melted fiber and etching its tip down to the Wl dimensions (V3 x a ), was positioned on top of the PhC-slab 960 as illustrated in Fig. 9. The arrangement allows the light to leak out of the taper 930 and to evanescently couple into Wl 970.
  • the PhC modes propagate in the waveguide 970 and interact with cavities 980 which leak the light vertically out of the slab.
  • a beam-splitter 920 redirected a fraction of the collimated beam to an IR camera 922 for imaging.
  • a field stop 916 was placed in front of the photodiode to locally probe lO ⁇ m-long waveguide sections and to block parts of the free-propagating beam deflected accidentally into the objective from surface impurities.
  • An arrangement of lenses 914 and 918 focuses the light on the photodector 912.
  • a typical scattering spectrum of the high Q-cavity is shown in the inset of Fig. 9.
  • the photonic crystal cavity can be used in similar fashion as a microsphere cavity to implement pump-probe spectroscopy by coupling several beams to the waveguide -microcavity system.
  • a disordered waveguide operated in the slow-light regime can be used in similar fashion. Please refer to provisional application for United States Letters Patent of Frank Vollmer and Juraj Topolancik for "Devices and Methods based on Strong Photon Localization in disordered photonic crystal waveguides" filed on 4th June 2007.

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Abstract

La présente invention concerne des microcavités optiques, des résonateurs à facteur de qualité élevée et des structures de lumière lente servant d'outils pour détecter des molécules, sonder des conformations et mesurer la polarisabilité et l'anisotropie de molécules et d'ensembles de molécules par l'intermédiaire d'une approche pompe-sonde. Les résonances sont excitées simultanément ou séquentiellement à l'aide de faisceaux pompe et sonde couplés à la même microcavité, de telle sorte qu'une longueur d'onde de faisceau pompe peut être choisie pour interagir avec les molécules adsorbées sur la surface de la microcavité, tandis qu'une longueur d'onde de faisceau sonde peut être choisie pour mesurer de manière non invasive des perturbations induites par la pompe. Les perturbations induites sont manifestement dues aux modifications des conditions de résonances et sont mesurées à partir des changements opérés dans les caractéristiques de transfert ou des changements du spectre de diffusion d'un système de guide d'ondes de microcavité. Les perturbations générées par le faisceau pompe peuvent être dues aux modifications de la polarisabilité, aux modifications de la conformation moléculaire, à la rupture ou à la formation de liaisons chimiques, au déclenchement d'états excités et à la formation de nouvelles espèces chimiques. En outre, l'absorption du faisceau pompe peut générer de la chaleur. De plus, le fait d'utiliser des modes résonants avec différents états de polarisation permet de mesurer la polarisabilité et son anisotropie dans des échantillons interagissant avec le dispositif optique.
PCT/US2007/078586 2006-09-15 2007-09-15 Procédés et dispositifs destinés à des mesures utilisant une spectroscopie pompe-sonde dans des microcavités de haute qualité WO2008034118A2 (fr)

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* Cited by examiner, † Cited by third party
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WO2008151224A1 (fr) * 2007-06-04 2008-12-11 President And Fellows Of Harvard College Système et procédé pour la localisation de photons forte par des structures de cristal photoniques désordonnées (quasi-cristaux)
US8102597B1 (en) * 2008-05-15 2012-01-24 Oewaves, Inc. Structures and fabrication of whispering-gallery-mode resonators
KR20120016297A (ko) * 2009-06-01 2012-02-23 코넬 유니버시티 미소구를 이용한 통합 광학유체 시스템
US8928883B1 (en) 2009-07-07 2015-01-06 Raytheon Company Optical device for detection of an agent
WO2012031208A2 (fr) * 2010-09-02 2012-03-08 University Of Delaware Détection ultrasensible de vapeurs chimiques par spectroscopie photothermique à microcavités
US8582104B2 (en) 2011-06-30 2013-11-12 Raytheon Company Optical device for detection of an agent
WO2013066447A1 (fr) 2011-08-01 2013-05-10 The Trustees Of Columbia University In The City Of New York Imageur plan sans lentille et émetteur sans fil
WO2013059665A1 (fr) 2011-10-19 2013-04-25 The Trustees Of Columbia University In The City Of New York Réseau fabry-perrot ultracompact pour imagerie hyperspectrale ultracompacte
WO2013176759A1 (fr) * 2012-03-30 2013-11-28 The Trustees Of Columbia University In The City Of New York Systèmes et procédés pour détecteur au silicium infrarouge à puce intégrée
WO2013148349A1 (fr) 2012-03-30 2013-10-03 The Trustees Of Columbia University In The City Of New York Photonique au graphène pour dispositifs électro-optiques améliorés par résonateur et interactions tout-optique
JP5342674B1 (ja) * 2012-05-27 2013-11-13 株式会社石原産業 細径化光ファイバ、その製造方法及びその製造装置
US9212948B2 (en) 2012-11-07 2015-12-15 The Trustees Of Columbia University In The City Of New York Lossless hyperspectral imaging
WO2014179414A1 (fr) * 2013-05-01 2014-11-06 Corning Incorporated Tige à ligne d'air à répartition aléatoire
US9395311B2 (en) 2013-09-12 2016-07-19 Kestrel Corporation Differential excitation spectroscopy
US9441480B2 (en) 2013-10-03 2016-09-13 Baker Hughes Incorporated Wavelength-selective, high temperature, near infrared photodetectors for downhole applications
US9535219B2 (en) * 2014-02-14 2017-01-03 Wisconsin Alumni Research Foundation Single molecule spectroscopy using photothermal tuning of optical microcavities
CN104597631B (zh) * 2014-09-29 2018-09-18 欧阳征标 一种引入三角引导柱的宽频带三端口光环行器
CN104950384B (zh) * 2014-09-29 2020-11-13 欧阳征标 圆孔式正方晶格光子晶体低折射率双补偿散射柱直角波导
WO2018039417A1 (fr) * 2016-08-24 2018-03-01 The Research Foundation For The State University Of New York Appareil et procédé de spectroscopie bidimensionnelle ultrarapide assistée par cavité
DE102017122163A1 (de) * 2017-09-25 2019-03-28 Universitätsklinikum Jena Bestimmung des Dipolmoments von Makromolekülen
US11073421B2 (en) * 2018-08-09 2021-07-27 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Methods and apparatuses for measuring optical radiation
CN113075594B (zh) * 2021-03-24 2022-04-19 北京航空航天大学 一种serf原子磁强计的电子极化率双轴原位测量系统及方法
US11988557B2 (en) 2021-12-08 2024-05-21 Battelle Savannah River Alliance, Llc Electric field detection method and system
CN114486792B (zh) * 2022-01-17 2023-08-08 吉林大学 一种基于近红外双波长光子晶体慢光波导的光热干涉光谱气体传感装置及检测方法
CN115265773B (zh) * 2022-08-29 2025-09-12 中国人民解放军军事科学院国防科技创新研究院 基于回音壁模式微腔的中远红外光宽谱探测系统
CN119376123A (zh) * 2024-10-18 2025-01-28 中国地质大学（武汉） 一种基于光学微腔中热效应的快慢光转换方法及装置

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4652120A (en) * 1984-09-10 1987-03-24 General Motors Corporation Photothermal method of measuring fluid velocity
US5436454A (en) * 1993-10-15 1995-07-25 Nicolet Instrument Corporation Optical probe for remote attenuated total reflectance measurements
US6011984A (en) * 1995-11-22 2000-01-04 Minimed Inc. Detection of biological molecules using chemical amplification and optical sensors
US6608716B1 (en) * 1999-05-17 2003-08-19 New Mexico State University Technology Transfer Corporation Optical enhancement with nanoparticles and microcavities
US6507684B2 (en) 2000-06-28 2003-01-14 The Charles Stark Draper Laboratory, Inc. Optical microcavity resonator system
US6490039B2 (en) * 2000-08-08 2002-12-03 California Institute Of Technology Optical sensing based on whispering-gallery-mode microcavity
US20040023396A1 (en) * 2001-11-14 2004-02-05 Boyd Robert W. Ring or disk resonator photonic biosensor and its use
US6896559B2 (en) * 2003-03-14 2005-05-24 Tyco Electronics Corporation Pin retention apparatus, methods and articles of manufacture
US7082147B2 (en) 2003-03-24 2006-07-25 Eastman Kodak Company Organic fiber laser system and method
US7257279B2 (en) * 2004-09-20 2007-08-14 3M Innovative Properties Company Systems and methods for biosensing and microresonator sensors for same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105676367A (zh) * 2016-04-20 2016-06-15 安徽大学 反馈式可调光学微腔延时方法及延时器
CN109557616A (zh) * 2016-04-20 2019-04-02 安徽大学 反馈式可调光学微腔延时器
CN109557616B (zh) * 2016-04-20 2021-03-30 安徽大学 异侧耦合反馈式可调光学微腔延时器

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