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WO2008035991A3 - A nucleic acid extraction method - Google Patents

A nucleic acid extraction method Download PDF

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Publication number
WO2008035991A3
WO2008035991A3 PCT/NZ2007/000273 NZ2007000273W WO2008035991A3 WO 2008035991 A3 WO2008035991 A3 WO 2008035991A3 NZ 2007000273 W NZ2007000273 W NZ 2007000273W WO 2008035991 A3 WO2008035991 A3 WO 2008035991A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
extraction method
acid extraction
affinity matrix
suspension
Prior art date
Application number
PCT/NZ2007/000273
Other languages
French (fr)
Other versions
WO2008035991A2 (en
Inventor
Michael Ronald Cook
Original Assignee
Michael Ronald Cook
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michael Ronald Cook filed Critical Michael Ronald Cook
Publication of WO2008035991A2 publication Critical patent/WO2008035991A2/en
Publication of WO2008035991A3 publication Critical patent/WO2008035991A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides a method of isolating nucleic acid from biological material, the method comprising the steps: a) providing biological material suspended in a solution, b) adjusting the pH of the suspension from a) to within the range about pH 1 to about pH 6, c) contacting nucleic acid in the suspension from b) with an affinity matrix capable of binding the nucleic acid in the pH range about pH 1 to about pH 6, in order to bind the nucleic acid to the affinity matrix; and d) eluting the nucleic acid from the affinity matrix using a buffer with a pH of more than 8, wherein the method does not require a centrifugation or filtration step prior to step c).
PCT/NZ2007/000273 2006-09-19 2007-09-19 A nucleic acid extraction method WO2008035991A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NZ54998306 2006-09-19
NZ549983 2006-09-19

Publications (2)

Publication Number Publication Date
WO2008035991A2 WO2008035991A2 (en) 2008-03-27
WO2008035991A3 true WO2008035991A3 (en) 2008-07-10

Family

ID=39200992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NZ2007/000273 WO2008035991A2 (en) 2006-09-19 2007-09-19 A nucleic acid extraction method

Country Status (1)

Country Link
WO (1) WO2008035991A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0814570D0 (en) 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
DE102008063001A1 (en) * 2008-12-23 2010-06-24 Qiagen Gmbh Nucleic acid purification method
JP5899731B2 (en) 2011-09-13 2016-04-06 ソニー株式会社 Nucleic acid purification method, nucleic acid extraction method, and nucleic acid purification kit
WO2014052551A1 (en) * 2012-09-28 2014-04-03 Cepheid Methods for dna and rna extraction from fixed paraffin-embedded tissue samples
EP3705582B1 (en) 2015-07-24 2025-09-03 Cepheid Compositions and methods for dna and rna extraction from tissue samples
US20240192100A1 (en) * 2021-04-01 2024-06-13 Fujirebio Inc. Method for treating biological sample

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999029703A2 (en) * 1997-12-06 1999-06-17 Dna Research Instruments Limited Isolation of nucleic acids
WO2002048164A2 (en) * 2000-12-14 2002-06-20 Dna Research Innovations Limited Isolation of nucleic acids
WO2004055213A1 (en) * 2002-12-16 2004-07-01 Dna Research Innovations Limited Tagged polyfunctional reagents capable of reversibly binding target substances in a ph-dependent manner
WO2004056984A2 (en) * 2002-12-18 2004-07-08 University Of Geneva Enzymatic method for the isolation of dna from plant tissue

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999029703A2 (en) * 1997-12-06 1999-06-17 Dna Research Instruments Limited Isolation of nucleic acids
WO2002048164A2 (en) * 2000-12-14 2002-06-20 Dna Research Innovations Limited Isolation of nucleic acids
WO2004055213A1 (en) * 2002-12-16 2004-07-01 Dna Research Innovations Limited Tagged polyfunctional reagents capable of reversibly binding target substances in a ph-dependent manner
WO2004056984A2 (en) * 2002-12-18 2004-07-08 University Of Geneva Enzymatic method for the isolation of dna from plant tissue

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"ChargeSwitch gDNA Plant Kit: Instruction Manual", INVITROGEN LIFE TECHNOLOGIES, VERSION B, 27 April 2005 (2005-04-27) *
KI C.S. ET AL.: "A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP", NUCLEIC ACIDS RES., vol. 25, no. 5, 1997, pages 1085 - 1086 *
KOONJUL P.K. ET AL.: "Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA", NUCLEIC ACIDS RES., vol. 27, no. 3, 1999, pages 915 - 916, XP002348231, DOI: doi:10.1093/nar/27.3.915 *

Also Published As

Publication number Publication date
WO2008035991A2 (en) 2008-03-27

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