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WO2008118998A3 - Method for multiplex detection and quantitation of nucleic acids - Google Patents

Method for multiplex detection and quantitation of nucleic acids Download PDF

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Publication number
WO2008118998A3
WO2008118998A3 PCT/US2008/058333 US2008058333W WO2008118998A3 WO 2008118998 A3 WO2008118998 A3 WO 2008118998A3 US 2008058333 W US2008058333 W US 2008058333W WO 2008118998 A3 WO2008118998 A3 WO 2008118998A3
Authority
WO
WIPO (PCT)
Prior art keywords
quantitation
nucleic acids
multiplex detection
sample
methods
Prior art date
Application number
PCT/US2008/058333
Other languages
French (fr)
Other versions
WO2008118998A2 (en
Inventor
Vladimir Slepnev
John Unger
Elizabeth Garcia
Kyle Hart
Benjamin Stone
Original Assignee
Primera Biosystems Inc
Vladimir Slepnev
John Unger
Elizabeth Garcia
Kyle Hart
Benjamin Stone
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Primera Biosystems Inc, Vladimir Slepnev, John Unger, Elizabeth Garcia, Kyle Hart, Benjamin Stone filed Critical Primera Biosystems Inc
Publication of WO2008118998A2 publication Critical patent/WO2008118998A2/en
Publication of WO2008118998A3 publication Critical patent/WO2008118998A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed herein are methods for the quantitative detection of target sequences in a sample. The methods can be used for multiplex quantitative SNP detection to determine changes in mutation profiles over time in a sample. For example, mutations that arise as a result of therapeutic intervention such as chemotherapy for the treatment of cancer.
PCT/US2008/058333 2007-03-27 2008-03-27 Method for multiplex detection and quantitation of nucleic acids WO2008118998A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92023307P 2007-03-27 2007-03-27
US60/920,233 2007-03-27

Publications (2)

Publication Number Publication Date
WO2008118998A2 WO2008118998A2 (en) 2008-10-02
WO2008118998A3 true WO2008118998A3 (en) 2008-11-27

Family

ID=39713869

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/058333 WO2008118998A2 (en) 2007-03-27 2008-03-27 Method for multiplex detection and quantitation of nucleic acids

Country Status (1)

Country Link
WO (1) WO2008118998A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10131951B2 (en) 2010-08-06 2018-11-20 Ariosa Diagnostics, Inc. Assay systems for genetic analysis
US10233496B2 (en) 2010-08-06 2019-03-19 Ariosa Diagnostics, Inc. Ligation-based detection of genetic variants
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10718019B2 (en) 2011-01-25 2020-07-21 Ariosa Diagnostics, Inc. Risk calculation for evaluation of fetal aneuploidy
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2280079A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Ligation-based method of normalized quantification of nucleic acids
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
AU2015201175B2 (en) * 2010-08-06 2017-09-07 F. Hoffmann-La Roche Ag Assay systems for determination of source contribution in a sample
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
EP4361606A3 (en) 2012-02-03 2024-07-10 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
CA2878280A1 (en) 2012-07-19 2014-01-23 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
IN2015DN01609A (en) 2012-08-03 2015-07-03 California Inst Of Techn
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
DE102018213027A1 (en) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaction mixture, method and kit for carrying out a quantitative real-time PCR

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045559A1 (en) * 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
EP1319718A1 (en) * 2001-12-14 2003-06-18 Keygene N.V. High throughput analysis and detection of multiple target sequences

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045559A1 (en) * 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
EP1319718A1 (en) * 2001-12-14 2003-06-18 Keygene N.V. High throughput analysis and detection of multiple target sequences

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRINSON E C ET AL: "INTRODUCTION TO PCR/OLA/SCS, A MULTIPLEX DNA TEST, AND ITS APPLICATION TO CYSTIC FIBROSIS", GENETIC TESTING, LARCHMONT, NY, US, vol. 1, no. 1, 1 January 1997 (1997-01-01), pages 61 - 68, XP009057884, ISSN: 1090-6576 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10131951B2 (en) 2010-08-06 2018-11-20 Ariosa Diagnostics, Inc. Assay systems for genetic analysis
US10233496B2 (en) 2010-08-06 2019-03-19 Ariosa Diagnostics, Inc. Ligation-based detection of genetic variants
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10718019B2 (en) 2011-01-25 2020-07-21 Ariosa Diagnostics, Inc. Risk calculation for evaluation of fetal aneuploidy
US10718024B2 (en) 2011-01-25 2020-07-21 Ariosa Diagnostics, Inc. Risk calculation for evaluation of fetal aneuploidy

Also Published As

Publication number Publication date
WO2008118998A2 (en) 2008-10-02

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