WO2008128144A3 - Monoclonal antibody selecting system, and making and using thereof - Google Patents
Monoclonal antibody selecting system, and making and using thereof Download PDFInfo
- Publication number
- WO2008128144A3 WO2008128144A3 PCT/US2008/060165 US2008060165W WO2008128144A3 WO 2008128144 A3 WO2008128144 A3 WO 2008128144A3 US 2008060165 W US2008060165 W US 2008060165W WO 2008128144 A3 WO2008128144 A3 WO 2008128144A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cells
- transgenic
- chimeric protein
- monoclonal antibody
- domain
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 abstract 4
- 241000699660 Mus musculus Species 0.000 abstract 3
- 206010035226 Plasma cell myeloma Diseases 0.000 abstract 3
- 102000037865 fusion proteins Human genes 0.000 abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 3
- 201000000050 myeloid neoplasm Diseases 0.000 abstract 3
- 230000009261 transgenic effect Effects 0.000 abstract 3
- 238000011830 transgenic mouse model Methods 0.000 abstract 3
- 210000000628 antibody-producing cell Anatomy 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108050001049 Extracellular proteins Proteins 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 abstract 1
- 210000003719 b-lymphocyte Anatomy 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000009877 rendering Methods 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
- A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- Animal Husbandry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention provides a DNA encoding a chimeric protein that comprises one transmembrane domain and one extracellular Fc-binding domain and provides transgenic myeloma cells and transgenic mice having the DNA integrated into their genome. The invention also provides methods of preparing transgenic myeloma cell and transgenic mice expressing the chimeric protein. The invention further provides methods of using the transgenic myeloma cells and transgenic mice said mice to facilitate isolation of monoclonal antibodies-producing B-cells. Specifically, antibody-producing cells are engineered to express a chimeric protein that comprises a transmembrane domain and an extracellular Protein G' domain, rendering the cells binding to antibodies released by themselves. Such character simplifies the subsequent selection of monoclonal antibody-producing cells.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US91188907P | 2007-04-15 | 2007-04-15 | |
US60/911,889 | 2007-04-15 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2008128144A2 WO2008128144A2 (en) | 2008-10-23 |
WO2008128144A3 true WO2008128144A3 (en) | 2009-02-12 |
Family
ID=39864636
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/060165 WO2008128144A2 (en) | 2007-04-15 | 2008-04-14 | Monoclonal antibody selecting system, and making and using thereof |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2008128144A2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8518392B2 (en) | 2009-08-14 | 2013-08-27 | Regeneron Pharmaceuticals, Inc. | Promoter-regulated differentiation-dependent self-deleting cassette |
EP3221455B1 (en) | 2014-11-20 | 2020-08-12 | Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. | Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells |
CN114134163A (en) * | 2021-11-05 | 2022-03-04 | 中国农业大学 | Single antigen specific transgenic hybridoma cell screening method |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6180343B1 (en) * | 1998-10-08 | 2001-01-30 | Rigel Pharmaceuticals, Inc. | Green fluorescent protein fusions with random peptides |
US20030162947A1 (en) * | 2001-02-20 | 2003-08-28 | Meagher Richard B. | Rapid production of monoclonal antibodies |
US20060228710A1 (en) * | 2003-02-14 | 2006-10-12 | Morris David W | Novel therapeutic targets in cancer |
US7166423B1 (en) * | 1992-10-21 | 2007-01-23 | Miltenyi Biotec Gmbh | Direct selection of cells by secretion product |
-
2008
- 2008-04-14 WO PCT/US2008/060165 patent/WO2008128144A2/en active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7166423B1 (en) * | 1992-10-21 | 2007-01-23 | Miltenyi Biotec Gmbh | Direct selection of cells by secretion product |
US6180343B1 (en) * | 1998-10-08 | 2001-01-30 | Rigel Pharmaceuticals, Inc. | Green fluorescent protein fusions with random peptides |
US20030162947A1 (en) * | 2001-02-20 | 2003-08-28 | Meagher Richard B. | Rapid production of monoclonal antibodies |
US20060228710A1 (en) * | 2003-02-14 | 2006-10-12 | Morris David W | Novel therapeutic targets in cancer |
Also Published As
Publication number | Publication date |
---|---|
WO2008128144A2 (en) | 2008-10-23 |
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