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WO2009005564A3 - Séquences nucléotidiques codant pour l'enzyme dégradant la cellulose et l'hémicellulose et ayant une cinétique traductionnelle raffinée, et procédé de production correspondant - Google Patents

Séquences nucléotidiques codant pour l'enzyme dégradant la cellulose et l'hémicellulose et ayant une cinétique traductionnelle raffinée, et procédé de production correspondant Download PDF

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Publication number
WO2009005564A3
WO2009005564A3 PCT/US2008/006379 US2008006379W WO2009005564A3 WO 2009005564 A3 WO2009005564 A3 WO 2009005564A3 US 2008006379 W US2008006379 W US 2008006379W WO 2009005564 A3 WO2009005564 A3 WO 2009005564A3
Authority
WO
WIPO (PCT)
Prior art keywords
hemicellulose
cellulose
expression
degradation enzyme
encoding nucleotide
Prior art date
Application number
PCT/US2008/006379
Other languages
English (en)
Other versions
WO2009005564A2 (fr
Inventor
Kirsty A Salmon
David A Roth
Wesley G Hatfield
Yimeng Dou
Original Assignee
Univ California
Kirsty A Salmon
David A Roth
Wesley G Hatfield
Yimeng Dou
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Kirsty A Salmon, David A Roth, Wesley G Hatfield, Yimeng Dou filed Critical Univ California
Publication of WO2009005564A2 publication Critical patent/WO2009005564A2/fr
Publication of WO2009005564A3 publication Critical patent/WO2009005564A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne des séquences de polynucléotides et de gènes synthétiques, codant pour des enzyme dégradant la cellulose et l'hémicellulose, aptes à s'exprimer dans un organisme hôte et ayant une cinétique traductionnelle améliorée et/ou raffinée, ainsi que des procédés de fabrication correspondant. Le nucléotide ainsi obtenu codant pour l'enzyme dégradant la cellulose et l'hémicellulose devrait être traduit rapidement sur toute sa longueur. L'expression du nucléotide codant pour l'enzyme dégradant la cellulose et l'hémicellulose résultant devrait générer des niveaux d'expression protéique améliorés dans les cas où des pauses de traduction inappropriées ou excessives réduisent l'expression de la protéine. En outre, l'expression du nucléotide codant pour l'enzyme dégradant la cellulose et l'hémicellulose résultant devrait générer des niveaux d'expression des polypeptides actifs et/ou repliés sous forme native et fonctionnels améliorés dans les cas où des pauses de traduction inappropriées ou excessives induisent l'expression d'une enzyme dégradant le cellulose et l'hémicellulose inactive, insoluble, agglomérée ou dysfonctionnelle ou très peu active.
PCT/US2008/006379 2007-06-29 2008-05-14 Séquences nucléotidiques codant pour l'enzyme dégradant la cellulose et l'hémicellulose et ayant une cinétique traductionnelle raffinée, et procédé de production correspondant WO2009005564A2 (fr)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US94708607P 2007-06-29 2007-06-29
US94732907P 2007-06-29 2007-06-29
US94727707P 2007-06-29 2007-06-29
US60/947,086 2007-06-29
US60/947,329 2007-06-29
US60/947,277 2007-06-29
US94749607P 2007-07-02 2007-07-02
US94761707P 2007-07-02 2007-07-02
US60/947,496 2007-07-02
US60/947,617 2007-07-02
US94778407P 2007-07-03 2007-07-03
US60/947,784 2007-07-03

Publications (2)

Publication Number Publication Date
WO2009005564A2 WO2009005564A2 (fr) 2009-01-08
WO2009005564A3 true WO2009005564A3 (fr) 2009-03-05

Family

ID=39734194

Family Applications (1)

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PCT/US2008/006379 WO2009005564A2 (fr) 2007-06-29 2008-05-14 Séquences nucléotidiques codant pour l'enzyme dégradant la cellulose et l'hémicellulose et ayant une cinétique traductionnelle raffinée, et procédé de production correspondant

Country Status (1)

Country Link
WO (1) WO2009005564A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8686123B2 (en) * 2010-11-15 2014-04-01 Edeniq, Inc. Use of manganese peroxidase for enzymatic hydrolysis of lignocellulosic material
EP2885404B1 (fr) * 2012-08-16 2018-04-25 Bangladesh Jute Research Institute Enzymes d'altération de la lignine provenant de macrophomia phaseolina et utilisations de celles-ci
CN104388441B (zh) * 2014-10-30 2017-11-14 上海市农业科学院 来源于瓜类炭疽病菌的漆酶基因及其应用
CN104357414B (zh) * 2014-11-28 2017-11-14 上海市农业科学院 来源于双色蜡蘑的漆酶基因及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5082767A (en) * 1989-02-27 1992-01-21 Hatfield G Wesley Codon pair utilization
WO2003070957A2 (fr) * 2002-02-20 2003-08-28 Novozymes A/S Production de polypeptides de plantes
WO2007130606A2 (fr) * 2006-05-04 2007-11-15 The Regents Of The University Of California Analyse de cinétique translationnelle utilisant des afficheurs graphiques de valeurs cinétiques translationnelles de paires de codon
WO2008000632A1 (fr) * 2006-06-29 2008-01-03 Dsm Ip Assets B.V. Procédé pour obtenir une expression de polypeptides améliorée

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5082767A (en) * 1989-02-27 1992-01-21 Hatfield G Wesley Codon pair utilization
WO2003070957A2 (fr) * 2002-02-20 2003-08-28 Novozymes A/S Production de polypeptides de plantes
WO2007130606A2 (fr) * 2006-05-04 2007-11-15 The Regents Of The University Of California Analyse de cinétique translationnelle utilisant des afficheurs graphiques de valeurs cinétiques translationnelles de paires de codon
WO2007130650A2 (fr) * 2006-05-04 2007-11-15 The Regents Of The University Of California Procédés de calcul de valeurs cinétiques translationelles à base de paire de codons, et procédés de production de séquences nucléotidiques codant pour un polypeptide à partir de ces valeurs
WO2008000632A1 (fr) * 2006-06-29 2008-01-03 Dsm Ip Assets B.V. Procédé pour obtenir une expression de polypeptides améliorée

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 25 November 2003 (2003-11-25), "Pycnoporus sanguineus laccase mRNA, complete cds.", XP002496141, retrieved from EBI accession no. EMBL:AY458017 Database accession no. AY458017 *
GONZALEZ ET AL: "Identification of a new laccase gene and confirmation of genomic predictions by cDNA sequences of Trametes sp. I-62 laccase family", MYCOLOGICAL RESEARCH, ELSEVIER, GB, vol. 107, no. 6, 1 June 2003 (2003-06-01), pages 727 - 735, XP022443249, ISSN: 0953-7562 *
GUSTAFSSON C ET AL: "Codon bias and heterologous protein expression", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 22, no. 7, 1 July 2004 (2004-07-01), pages 346 - 353, XP004520507, ISSN: 0167-7799 *
HATFIELD G WESLEY ET AL: "Optimizing scaleup yield for protein production: Computationally Optimized DNA Assembly (CODA) and Translation Engineering(TM)", BIOTECHNOLOGY ANNUAL REVIEW, XX, XX, vol. 13, 1 January 2007 (2007-01-01), pages 27 - 42, XP009092735 *
HATFIELD G.W. ET AL.: "Codon pair utilization bias in bacteria, yeast and mammals", 1993, CRC PRESS, BOCA RATON, LA, XP002495824 *
IRWIN B ET AL: "codon pair utilization biases influence translational elongation step times", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,; US, vol. 270, no. 39, 29 September 1995 (1995-09-29), pages 22801 - 22806, XP002406003, ISSN: 0021-9258 *
KITTLE J.D., JR: "Radical Changes in the Engineering of Synthetic Genes for Protein Expression", BIOPHARM INTERNATIONAL, February 2006 (2006-02-01), XP002495822, Retrieved from the Internet <URL:http://www.codagenomics.com/technology/pubs/Biopharm_pdf_BP3-61-06e.pdf> [retrieved on 20080915] *
TRINH RYAN ET AL: "Optimization of codon pair use within the (GGGGS)3 linker sequence results in enhanced protein expression.", MOLECULAR IMMUNOLOGY, vol. 40, no. 10, January 2004 (2004-01-01), pages 717 - 722, XP002495823, ISSN: 0161-5890 *

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Publication number Publication date
WO2009005564A2 (fr) 2009-01-08

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