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WO2009034842A1 - 核酸検出方法、および核酸検出キット - Google Patents

核酸検出方法、および核酸検出キット Download PDF

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Publication number
WO2009034842A1
WO2009034842A1 PCT/JP2008/065307 JP2008065307W WO2009034842A1 WO 2009034842 A1 WO2009034842 A1 WO 2009034842A1 JP 2008065307 W JP2008065307 W JP 2008065307W WO 2009034842 A1 WO2009034842 A1 WO 2009034842A1
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid detection
substance
dna
dna amplification
Prior art date
Application number
PCT/JP2008/065307
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English (en)
French (fr)
Inventor
Jun Tomono
Original Assignee
Kaneka Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corporation filed Critical Kaneka Corporation
Priority to US12/677,189 priority Critical patent/US9063130B2/en
Priority to JP2009532131A priority patent/JPWO2009034842A1/ja
Priority to EP08831240.0A priority patent/EP2208795B1/en
Publication of WO2009034842A1 publication Critical patent/WO2009034842A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

　第１物質が特異的に結合することが可能な第１物質結合部位を含む、核酸増幅法によるＤＮＡ増幅断片を用意する。該ＤＮＡ増幅断片を上記第１物質に結合させることによって、該ＤＮＡ増幅断片を濃縮する。ＤＮＡは、濃縮により高感度に検出することができる。したがって、上記構成によれば、核酸増幅法によるＤＮＡ増幅断片を、特殊な装置を必要とすることなく、簡便かつ高精度に検出することができる。
PCT/JP2008/065307 2007-09-11 2008-08-27 核酸検出方法、および核酸検出キット WO2009034842A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/677,189 US9063130B2 (en) 2007-09-11 2008-08-27 Nucleic acid detection method and nucleic acid detection kit
JP2009532131A JPWO2009034842A1 (ja) 2007-09-11 2008-08-27 核酸検出方法、および核酸検出キット
EP08831240.0A EP2208795B1 (en) 2007-09-11 2008-08-27 Nucleic acid detection method, and nucleic acid detection kit

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-235935 2007-09-11
JP2007235935 2007-09-11

Publications (1)

Publication Number Publication Date
WO2009034842A1 true WO2009034842A1 (ja) 2009-03-19

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/065307 WO2009034842A1 (ja) 2007-09-11 2008-08-27 核酸検出方法、および核酸検出キット

Country Status (4)

Country Link
US (1) US9063130B2 (ja)
EP (1) EP2208795B1 (ja)
JP (2) JPWO2009034842A1 (ja)
WO (1) WO2009034842A1 (ja)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012070618A1 (ja) * 2010-11-24 2012-05-31 株式会社カネカ 増幅核酸検出方法及び検出デバイス
CN102934322A (zh) * 2010-06-09 2013-02-13 日产自动车株式会社 电池充电系统
WO2013039228A1 (ja) 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
WO2014007289A1 (ja) 2012-07-05 2014-01-09 日本碍子株式会社 核酸クロマトグラフィー法、核酸クロマトグラフィー用組成物及びこれを含むキット
US9783844B2 (en) 2012-04-27 2017-10-10 Kaneka Corporation Method for amplifying nucleic acid and method for detecting amplified nucleic acid
US10392652B2 (en) 2013-11-22 2019-08-27 Kaneka Corporation Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end
WO2024232313A1 (ja) * 2023-05-08 2024-11-14 国立研究開発法人産業技術総合研究所 検出装置と検出方法と加温装置

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103119178B (zh) * 2010-09-22 2017-05-17 株式会社钟化 核酸的检测方法以及装置、试剂盒
US9273174B2 (en) 2011-03-04 2016-03-01 Kaneka Corporation Nucleic acid detection method, and device and kit for use in same
DE102015115836A1 (de) * 2015-09-18 2017-03-23 Biotype Diagnostic Gmbh Bestätigungstest für primäre Nukleinsäure-Amplifikate in einem kontinuierlichen Reaktionsansatz und unmittelbare Auswertung mittels immunchromatographischer Verfahren

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JP2002186481A (ja) 2000-12-18 2002-07-02 Eiken Chem Co Ltd 光学的特性の測定による核酸増幅産物の検出方法
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102934322A (zh) * 2010-06-09 2013-02-13 日产自动车株式会社 电池充电系统
CN102934322B (zh) * 2010-06-09 2015-04-08 日产自动车株式会社 电池充电系统
WO2012070618A1 (ja) * 2010-11-24 2012-05-31 株式会社カネカ 増幅核酸検出方法及び検出デバイス
US9920356B2 (en) 2010-11-24 2018-03-20 Kaneka Corporation Amplified nucleic acid detection method and detection device
US10829805B2 (en) 2010-11-24 2020-11-10 Kaneka Corporation Amplified nucleic acid detection method and detection device
WO2013039228A1 (ja) 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
US9783844B2 (en) 2012-04-27 2017-10-10 Kaneka Corporation Method for amplifying nucleic acid and method for detecting amplified nucleic acid
WO2014007289A1 (ja) 2012-07-05 2014-01-09 日本碍子株式会社 核酸クロマトグラフィー法、核酸クロマトグラフィー用組成物及びこれを含むキット
US10392652B2 (en) 2013-11-22 2019-08-27 Kaneka Corporation Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end
WO2024232313A1 (ja) * 2023-05-08 2024-11-14 国立研究開発法人産業技術総合研究所 検出装置と検出方法と加温装置

Also Published As

Publication number Publication date
EP2208795B1 (en) 2016-03-30
US9063130B2 (en) 2015-06-23
EP2208795A4 (en) 2011-02-02
US20100330564A1 (en) 2010-12-30
JPWO2009034842A1 (ja) 2010-12-24
JP5727570B2 (ja) 2015-06-03
EP2208795A1 (en) 2010-07-21
JP2014000098A (ja) 2014-01-09

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