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WO2013019949A3 - Systems and methods for isolating and characterizing target materials of a suspension - Google Patents

Systems and methods for isolating and characterizing target materials of a suspension Download PDF

Info

Publication number
WO2013019949A3
WO2013019949A3 PCT/US2012/049325 US2012049325W WO2013019949A3 WO 2013019949 A3 WO2013019949 A3 WO 2013019949A3 US 2012049325 W US2012049325 W US 2012049325W WO 2013019949 A3 WO2013019949 A3 WO 2013019949A3
Authority
WO
WIPO (PCT)
Prior art keywords
suspension
tube
target materials
float
isolating
Prior art date
Application number
PCT/US2012/049325
Other languages
French (fr)
Other versions
WO2013019949A2 (en
Inventor
Ronald C. Seubert
Jackie STILWELL
Paul Goodwin
Martha Stone
Arturo BERNARDO
Original Assignee
Rarecyte, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rarecyte, Inc. filed Critical Rarecyte, Inc.
Publication of WO2013019949A2 publication Critical patent/WO2013019949A2/en
Publication of WO2013019949A3 publication Critical patent/WO2013019949A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • A61M1/029—Separating blood components present in distinct layers in a container, not otherwise provided for
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Systems and methods for isolating and characterizing various target materials of a suspension are disclosed. A suspension suspected of containing the target materials is added to a tube. A float with a specific gravity corresponding to that of the target material is inserted into the tube. The tube, float, and suspension are centrifuged together causing the various materials suspended in the suspension to separate into different layers along the axial length of the tube according to their specific gravities. The float and/or tube are configured to drive the various target materials to a region of space between the float and inner wall of the tube.
PCT/US2012/049325 2011-08-02 2012-08-02 Systems and methods for isolating and characterizing target materials of a suspension WO2013019949A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161514102P 2011-08-02 2011-08-02
US61/514,102 2011-08-02

Publications (2)

Publication Number Publication Date
WO2013019949A2 WO2013019949A2 (en) 2013-02-07
WO2013019949A3 true WO2013019949A3 (en) 2013-04-25

Family

ID=47629916

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/049325 WO2013019949A2 (en) 2011-08-02 2012-08-02 Systems and methods for isolating and characterizing target materials of a suspension

Country Status (1)

Country Link
WO (1) WO2013019949A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106404721B (en) * 2016-08-25 2019-05-03 吴小戈 A blood concentration detection device

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6911315B2 (en) * 1997-11-24 2005-06-28 David L. Rimm Method for the detection, identification, enumeration and confirmation of virally infected cells and other epitopically defined cells in whole blood
US7074577B2 (en) * 2002-10-03 2006-07-11 Battelle Memorial Institute Buffy coat tube and float system and method
US20100074803A1 (en) * 2002-10-03 2010-03-25 Thomas Haubert Buffy coat separator float system and method
US20100317106A1 (en) * 2009-06-16 2010-12-16 Levine Robert A Harvesting target materials from centrifuged suspensions
US20110097816A1 (en) * 2009-10-23 2011-04-28 Goodwin Paul C Methods for changing densities of non-target particles of a suspension

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6911315B2 (en) * 1997-11-24 2005-06-28 David L. Rimm Method for the detection, identification, enumeration and confirmation of virally infected cells and other epitopically defined cells in whole blood
US7074577B2 (en) * 2002-10-03 2006-07-11 Battelle Memorial Institute Buffy coat tube and float system and method
US20100074803A1 (en) * 2002-10-03 2010-03-25 Thomas Haubert Buffy coat separator float system and method
US20100317106A1 (en) * 2009-06-16 2010-12-16 Levine Robert A Harvesting target materials from centrifuged suspensions
US20110097816A1 (en) * 2009-10-23 2011-04-28 Goodwin Paul C Methods for changing densities of non-target particles of a suspension

Also Published As

Publication number Publication date
WO2013019949A2 (en) 2013-02-07

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