WO2013036574A4 - Modified forms of pseudomonas exotoxin a - Google Patents
Modified forms of pseudomonas exotoxin a Download PDFInfo
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- WO2013036574A4 WO2013036574A4 PCT/US2012/053868 US2012053868W WO2013036574A4 WO 2013036574 A4 WO2013036574 A4 WO 2013036574A4 US 2012053868 W US2012053868 W US 2012053868W WO 2013036574 A4 WO2013036574 A4 WO 2013036574A4
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- polypeptide
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- threonine
- amino acid
- fusion protein
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- 108700033844 Pseudomonas aeruginosa toxA Proteins 0.000 title claims abstract 18
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 86
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 86
- 238000006467 substitution reaction Methods 0.000 claims abstract 75
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 24
- 210000004027 cell Anatomy 0.000 claims abstract 15
- 210000001744 T-lymphocyte Anatomy 0.000 claims abstract 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract 2
- 229920001184 polypeptide Polymers 0.000 claims 85
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 27
- 239000004473 Threonine Substances 0.000 claims 27
- 108020001507 fusion proteins Proteins 0.000 claims 25
- 102000037865 fusion proteins Human genes 0.000 claims 25
- 238000000034 method Methods 0.000 claims 25
- 108091033319 polynucleotide Proteins 0.000 claims 25
- 102000040430 polynucleotide Human genes 0.000 claims 25
- 239000002157 polynucleotide Substances 0.000 claims 25
- 235000001014 amino acid Nutrition 0.000 claims 24
- 235000004279 alanine Nutrition 0.000 claims 21
- 239000013604 expression vector Substances 0.000 claims 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 20
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 18
- 229960000310 isoleucine Drugs 0.000 claims 16
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims 16
- 108020001756 ligand binding domains Proteins 0.000 claims 16
- 230000005764 inhibitory process Effects 0.000 claims 15
- 239000004475 Arginine Substances 0.000 claims 14
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims 14
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 14
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 13
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 12
- 235000003704 aspartic acid Nutrition 0.000 claims 12
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 12
- 201000010099 disease Diseases 0.000 claims 10
- 208000035475 disorder Diseases 0.000 claims 10
- 150000003588 threonines Chemical class 0.000 claims 10
- 239000004472 Lysine Substances 0.000 claims 9
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 9
- 150000001294 alanine derivatives Chemical class 0.000 claims 9
- 230000004568 DNA-binding Effects 0.000 claims 8
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 8
- 235000013922 glutamic acid Nutrition 0.000 claims 8
- 239000004220 glutamic acid Substances 0.000 claims 8
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 claims 8
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims 8
- 239000003814 drug Substances 0.000 claims 7
- 239000003446 ligand Substances 0.000 claims 7
- 239000008194 pharmaceutical composition Substances 0.000 claims 7
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 6
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 6
- 239000000427 antigen Substances 0.000 claims 6
- 102000036639 antigens Human genes 0.000 claims 6
- 108091007433 antigens Proteins 0.000 claims 6
- 239000012634 fragment Substances 0.000 claims 6
- 206010028980 Neoplasm Diseases 0.000 claims 5
- 229940024606 amino acid Drugs 0.000 claims 5
- 230000004071 biological effect Effects 0.000 claims 5
- 239000003085 diluting agent Substances 0.000 claims 5
- 239000003937 drug carrier Substances 0.000 claims 5
- 210000002865 immune cell Anatomy 0.000 claims 5
- 239000000546 pharmaceutical excipient Substances 0.000 claims 5
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 4
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 4
- 108091027981 Response element Proteins 0.000 claims 4
- 150000001413 amino acids Chemical class 0.000 claims 4
- 235000009582 asparagine Nutrition 0.000 claims 4
- 229960001230 asparagine Drugs 0.000 claims 4
- 238000006471 dimerization reaction Methods 0.000 claims 4
- 230000009851 immunogenic response Effects 0.000 claims 4
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 claims 4
- 230000014616 translation Effects 0.000 claims 4
- 201000011510 cancer Diseases 0.000 claims 3
- 206010006187 Breast cancer Diseases 0.000 claims 2
- 208000026310 Breast neoplasm Diseases 0.000 claims 2
- 239000004471 Glycine Substances 0.000 claims 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 2
- 150000001483 arginine derivatives Chemical class 0.000 claims 2
- 210000003719 b-lymphocyte Anatomy 0.000 claims 2
- 230000027455 binding Effects 0.000 claims 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 239000004474 valine Substances 0.000 claims 2
- 239000013598 vector Substances 0.000 claims 2
- 230000003612 virological effect Effects 0.000 claims 2
- JBFQOLHAGBKPTP-NZATWWQASA-N (2s)-2-[[(2s)-4-carboxy-2-[[3-carboxy-2-[[(2s)-2,6-diaminohexanoyl]amino]propanoyl]amino]butanoyl]amino]-4-methylpentanoic acid Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)C(CC(O)=O)NC(=O)[C@@H](N)CCCCN JBFQOLHAGBKPTP-NZATWWQASA-N 0.000 claims 1
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 claims 1
- 230000005730 ADP ribosylation Effects 0.000 claims 1
- 102100038080 B-cell receptor CD22 Human genes 0.000 claims 1
- 102100026094 C-type lectin domain family 12 member A Human genes 0.000 claims 1
- 101710188619 C-type lectin domain family 12 member A Proteins 0.000 claims 1
- 101000884305 Homo sapiens B-cell receptor CD22 Proteins 0.000 claims 1
- 101001057504 Homo sapiens Interferon-stimulated gene 20 kDa protein Proteins 0.000 claims 1
- 101001055144 Homo sapiens Interleukin-2 receptor subunit alpha Proteins 0.000 claims 1
- 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 claims 1
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 claims 1
- 101000884271 Homo sapiens Signal transducer CD24 Proteins 0.000 claims 1
- 101000801433 Homo sapiens Trophoblast glycoprotein Proteins 0.000 claims 1
- 102100026878 Interleukin-2 receptor subunit alpha Human genes 0.000 claims 1
- 102000003735 Mesothelin Human genes 0.000 claims 1
- 108090000015 Mesothelin Proteins 0.000 claims 1
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 claims 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 claims 1
- 102100038081 Signal transducer CD24 Human genes 0.000 claims 1
- 102100033579 Trophoblast glycoprotein Human genes 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims 1
- 230000022534 cell killing Effects 0.000 claims 1
- 230000003013 cytotoxicity Effects 0.000 claims 1
- 231100000135 cytotoxicity Toxicity 0.000 claims 1
- 239000000539 dimer Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 229950008579 ertumaxomab Drugs 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 claims 1
- 229940022353 herceptin Drugs 0.000 claims 1
- 108010089256 lysyl-aspartyl-glutamyl-leucine Proteins 0.000 claims 1
- 229960002087 pertuzumab Drugs 0.000 claims 1
- 238000001243 protein synthesis Methods 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 claims 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 claims 1
- 230000000638 stimulation Effects 0.000 claims 1
- 229960000575 trastuzumab Drugs 0.000 claims 1
- 239000013638 trimer Substances 0.000 claims 1
- 230000002163 immunogen Effects 0.000 abstract 3
- 101710112752 Cytotoxin Proteins 0.000 abstract 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 abstract 1
- 230000005867 T cell response Effects 0.000 abstract 1
- 231100000599 cytotoxic agent Toxicity 0.000 abstract 1
- 239000003145 cytotoxic factor Substances 0.000 abstract 1
- 239000002619 cytotoxin Substances 0.000 abstract 1
- 230000003389 potentiating effect Effects 0.000 abstract 1
- 210000004881 tumor cell Anatomy 0.000 abstract 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1077—Pentosyltransferases (2.4.2)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
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- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
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- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/02—Pentosyltransferases (2.4.2)
- C12Y204/02036—NAD(+)--diphthamide ADP-ribosyltransferase (2.4.2.36)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
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- General Health & Medical Sciences (AREA)
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Abstract
Pseudomonas exotoxin A or "PE" is a 66kD, highly potent, cytotoxic protein secreted by the bacterium Pseudomonas aeruginosa. Various forms of PE have been coupled to other proteins, such as antibodies, to generate therapeutically useful cytotoxin conjugates that selectively target cells of a desired phenotype (such as tumor cells). In the present invention, peptides spanning the sequence of an approximately 38kD form of Pseudomonas exotoxin A protein were analyzed for the presence of immunogenic CD4+ T cell epitopes. Six immunogenic T cell epitopes were identified. Residues were identified within each epitope for introduction of targeted amino acid substitutions to reduce or prevent immunogenic T-cell responses in PE molecules which may be administered to a heterologous host.
Claims
AMENDED CLAIMS
received by the International Bureau on 28 March 2013 (28.03.2013)
1. A polypeptide having at least one Pseudomonas exotoxin A (PE-A) biological activity, wherein said polypeptide comprises one or more amino acid
substitutions compared to a wild-type PE-A polypeptide, wherein said one or more amino acid substitutions is a substitution of a different amino acid at one or more positions corresponding to amino acid residues in the polypeptide of SEQ ID NO;l, wherein said substitution positions are selected from the group consisting of:
a) isoleucine (I) at position 141;
b) arginine (R) at position 146;
c) glycine (G) at position 147;
d) glutamine (Q) at position 149;
c) asparagine (N) at position 150;
f) threonine (T) at position 152;
g) valine (V) at position 189;
h) arginine (R) at position 192;
i) glutamine (Q) at position 194;
j) aspartic acid (D) at position 197;
k) serine (S) at position 241;
1) isoleucine (I) at position 321; and
m) glutamine (Q) at position 326. 3. The polypeptide of claim 1, wherein said one or more amino acid substitutions is a conservative amino acid substitution. 3. The polypeptide of claim 1, wherein said one or more amino acid substitutions is selected from the group consisting of:
a) isoleucine (I) at position 141 is substituted with alanine (A), threonine (T), or histidine (H);
b) arginine (R) at position 146 is substituted with glutamine (Q) or alanine (A);
c) glycine (G) at position 147 is substituted with serine (S);
d) glutamine (Q) at position 149 is substituted with threonine (T); e) asparagine (N) at position 150 is substituted with alanine (A);
f) threonine (T) at position 152 is substituted with alanine (A) or
arginine (R);
g) valine (V) at 189 is substituted with alanine (A);
h) arginine (R) at position 192 is substituted with alanine (A) or
glutamine (Q);
i) glutamine (Q) at position 194 is substituted with arginine (R);
j) aspartic acid (D) at position 197 is substituted with lysine (K);
k) serine (S) at position 241 is substituted with threonine (T), asparagine
(N), lysine (K), or proline (P);
1) isoleucine (I) at position 321 is substituted with alanine (A),
asparagine (N), histidine (H), threonine (T), or glutamine (Q); and m) glutamine (Q) at position 326 is substituted with glutamic acid (E),
4. The polypeptide of claim 1 , wherein said polypeptide comprises a substitution for isoleucine (I) at position 141, a substitution for threonine (T) at position 152, a substitution for arginine (R) at position 192, a substitution for aspartic acid (D) at position 197, a substitution for serine (S) at position 241, and a substitution for glutamine (Q) at position 326.
5. The polypeptide of claim 1, wherein said polypeptide comprises a substitution of threonine (T) or alanine (A) for isoleucine (I) at position 141, a substitution alanine (A) or arginine (R) for threonine (T) at position 152, a substitution of alanine (A) for arginine (R) at position 192, a substitution of lysine (K) for aspartic acid (D) at position 197, a substitution of threonine (T) for serine (S) at position 241, and a substitution of glutamic acid (E) for glutamine (Q) at position 326.
6. The polypeptide of claim 1 , wherein said polypeptide comprises a substitution of threonine (T) for isoleucine (I) at position 141, a substitution alanine (A) for
threonine (T) at position 152, a substitution of alanine (A) for arginine (R) at position 192, a substitution of lysine (K) for aspartic acid (D) at position 197, a substitution of threonine (T) for serine (S) at position 241, and a substitution of glutamic acid (E) for glutamine (Q) at position 326.
7. The polypeptide of claim 1 , wherein said polypeptide comprises a substitution of alanine (A) for isoleucine (I) at position 141 , a substitution alanine (A) for threonine (T) at position 152, a substitution of alanine (A) for arginine (R) at position 192, a substitution of lysine (K) for aspartic acid (D) at position 197, a substitution of threonine (T) for serine (S) at position 241, and a substitution of glutamic acid (E) for glutamine (Q) at position 326.
8. The polypeptide of claim 1, wherein said polypeptide comprises a substitution for isoleucine (I) at position 141 , a substitution for threonine (T) at position 152, a substitution for aspartic acid (D) at position 197, a substitution for serine (S) at position 241, and a substitution for glutamine (Q) at position 326.
9. The polypeptide of claim 1 , wherein said polypeptide comprises a substitution for isoleucine (I) at position 141, a substitution for threonine (T) at position 152, a substitution for arginine (R) at position 192, a substitution for aspartic acid (D) at position 197, and a substitution for serine (S) at position 241 ,
10. The polypeptide of claim 1, wherein said polypeptide comprises a substitution of alanine (A) or threonine (T) for isoleucine (I) at position 141, a substitution of arginine (R) or alanine (A) for threonine (T) at position 152, a substitution of lysine (K) for aspartic acid (D) at position 197, a substitution of threonine (T) for serine (S) at position 241, and a substitution of glutamic acid (E) for glutamine (Q) at position 326.
11. The polypeptide of claim 1, wherein said polypeptide comprises a substitution of alanine (A) for isoleucine (I) at position 141, a substitution of arginine (R) for threonine (T) at position 152, a substitution of lysine (K) for aspartic acid (D) at
position 197, a substitution of threonine (T) for serine (S) at position 241, and a substitution of glutamic acid (E) for glutamine (Q) at position 326.
12. The polypeptide of claim 1 , wherein said polypeptide comprises a substitution of alanine (A) for isoleucine (I) at position 141, a substitution of alanine (A) for threonine (T) at position 152, a substitution of lysine (K) for aspartic acid (D) at position 197, a substitution of threonine (T) for serine (S) at position 241, and a substitution of glutamic acid (E) for glutamine (Q) at position 326.
13. The polypeptide of claim 1 , wherein said polypeptide comprises a substitution of threonine (T) for isoleucine (I) at position 14] , a substitution of alanine (A) for threonine (T) at position 152, a substitution of lysine (K) for aspartic acid (D) at position 197, a substitution of threonine (T) for serine (S) at position 241, and a substitution of glutamic acid (E) for glutamine (Q) at position 326.
14. The polypeptide in any one of claims 1 to 13, wherein the at least one
Pseudomonas exotoxin A (PE-A) biological activity comprises the ability to inhibit in vitro transcription/translation compared to a corresponding wild-type or non-substituted PE-A polypeptide, wherein said ability to inhibit in vitro transcription/translation is in an amount selected from the group consisting of: a) at least 5% inhibition;
b) at least 10% inhibition;
c) at least 15% inhibition;
d) at least 20% inhibition;
e) at least 25% inhibition;
f) at least 30% inhibition;
g) at least 40% inhibition;
h) at least 50% inhibition;
i) at least 60% inhibition;
j) at least 70% inhibition;
k) at least 80% inhibition;
1) at least 90% inhibition;
m) at least 100% inhibition;
n) about 100% inhibition; and
o) 100% inhibition.
The polypeptide of claim 14, comprising a number of amino acid substitutions selected from the group consisting of:
a) 1 amino acid substitution;
b) 2 amino acid substitutions;
c) 3 amino acid substitutions;
d) 4 amino acid substitutions;
e) 5 amino acid substitutions; and
f) 6 amino acid substitutions.
16. The polypeptide of claim 15, wherein said polypeptide comprises one or more amino acid substitutions which prevent or reduce host immunogenic responses compared to the same polypeptide without said one or more amino acid substitutions.
17. The polypeptide of claim 16, wherein host immunogenic responses are prevented or reduced in a human host.
18. The polypeptide of claim 17, wherein the last five or six amino acids in said polypeptide comprise one or more amino acid sequences selected from the group consisting of;
a) Arg-Glu-Asp-Leu-Lys;
b) Arg-Glu-Asp-Leu;
c) Lys-Asp-Glu-Leu;
d) Glu-Asp-Leu-Lys; and
e) a dimer, trimer, pentamer, hexamer, septamer, or octamer of (a), (b), (c) or (d), or any combination thereof.
19. The polypeptide of claim 18, wherein said polypeptide has one or more biological activities selected from the group consisting of:
a) eukaryotic cell killing activity (cell cytotoxicity); b) inhibits translation elongation factor EF-2 biological activity; c) induces or catalyzes ADP-ribosylation of EF-2; and d) inhibits protein synthesis.
20. The polypeptide of claim 19, wherein said one or more amino acid substitutions prevent or reduce host immunogenic responses compared to the same polypeptide without the corresponding said one or more amino acid substitutions.
21. A polypeptide comprising a biologically active fragment of the polypeptide of claim 20.
A polypeptide comprising a variant or derivative of the polypeptide of claim 20, wherein said variant or derivative shares amino acid sequence identity with the polypeptide of claim 20, wherein said shared amino acid sequence identity is selected from the group consisting of:
a) at least 80% identity;
b) at least 85% identity;
c) at least 90% identity;
d) at least 95% identity;
e) at least 97% identity;
f) at least 98% identity; and
g) at least 99% identity.
The polypeptide of claim 20, wherein said one or more amino acid substitutions prevent or reduce host immunogenic responses compared to a polypeptide comprising an amino acid sequence selected from the group consisting of:
a) SEQ ID NO:l;
b) SEQ ID NO:4;
SEQ ID NO: 133; and
d) SEQ ID NO:134.
24. The polypeptide of claim 20, wherein said polypeptide is a fusion protein,
25. The fusion protein of claim 24, wherein the amino-terminal end of said
polypeptide is fused to the carboxyl-terminal end of a different polypeptide.
26. The fusion protein of claim 24, wherein the carboxyl-terminal end of said
polypeptide is fused to the amino-terminal end of a different polypeptide.
27. The fusion protein of claim 24, wherein said protein comprises an antigen binding moiety,
28. The fusion protein of claim 27, wherein said antigen binding moiety is an
antibody or fragment thereof.
29. The fusion protein of claim 28, wherein said antibody, or fragment thereof, is an antibody selected from the list in Table 1, or is a fragment thereof,
30. The fusion protein of claim 28, wherein said antibody, or fragment thereof, specifically binds to a cancer-specific or tumor-specific antigen.
31. The fusion protein of claim 30, wherein said cancer-specific or tumor-specific antigen is a breast cancer antigen,
32. The fusion protein of claim 31 , wherein said breast cancer antigen is HER2.
33. The fusion protein of claim 28, wherein said antibody, or fragment thereof is selected from the group consisting of:
a) ERTUMAXOMAB (Rexomun);
b) PERTUZUMAB (Omnitarg); and
c) TRASTUZUMAB (Herceptin).
The fusion protein of claim 24, wherein said fusion protein comprises polypeptide selected from the group consisting of:
a) Mesothelin;
b) CD24;
o) CD22;
d) CD25;
e) CD 174;
f) TPBG;
g) CD56; and
h) C-type lectin-like molecule-1,
35. A polynucleotide encoding the polypeptide in any one of claims 1 to 13.
36. An expression vector comprising the polynucleotide of claim 35.
37. A host cell comprising the expression vector of claim 36.
38. A method of producing the polypeptide in any one of claims 1 to 13, wherein said method comprises:
a) obtaining a host cell comprising a polynucleotide encoding said
polypeptide;
b) exposing said host cell to conditions wherein said polypeptide.
39. A method of producing the polypeptide in any one of claims 1 to 13, wherein said method comprises use of an expression system comprising:
a) a first polynucleotide encoding a first hybrid polypeptide
comprising:
(i) a first ligand binding domain; and
(ii) a DNA-binding domain;
b) a second polynucleotide encoding a second hybrid polypeptide comprising:
(i) a second ligand binding domain; and
(ii) a transactivation domain;
c) a third polynucleotide encoding the polypeptide in any one of claims 1 to 13, wherein said third polynucleotide is operably associated with a response element capable of being bound by the DNA-binding domain of said first hybrid polypeptide;
wherein the first ligand binding domain and the second ligand binding domain are capable of ligand-induced dimerization,
wherein expression of the polypeptide in any one of claims 1 to 13 is modulated by a ligand which induces dimerization of said first and said second ligand binding domains,
wherein the polypeptide in any one of claims 1 to 13 is produced by allowing said ligand to contact said first and said second ligand binding domains.
40. A single expression vector or two or more expression vectors comprising:
a) a first polynucleotide encoding a first hybrid polypeptide
comprising:
(j) a first ligand binding domain; and
(ii) a DNA-binding domain;
b) a second polynucleotide encoding a second hybrid polypeptide comprising:
(i) a second ligand binding domain; and
(ii) a transactivation domain;
c) a third polynucleotide encoding the polypeptide in any one of claims 1 to 13, wherein said third polynucleotide is operably associated with a response element capable of being bound by the DNA-binding domain of said first hybrid polypeptide.
41. The expression vector or expression vectors of claim 40, wherein one or more of the vectors is a viral expression vector.
42. A host cell comprising the expression vector or expression vectors of claim 40.
43. A method of treating a disease or disorder comprising administering to a subject in need thereof the polypeptide in any one of claims 1 to 13.
44. A method of treating a disease or disorder comprising delivering to a subject in need thereof a polypeptide produced by the method of claim 39, wherein said method comprises administration of the ligand to said subject,
45. The method of claim 44, wherein the polypeptide is delivered to the subject by first administering the first, second, and third polynucleotides,
46. A method of treating a disease or disorder comprising administering to a subject in need thereof the expression vector of claim 36.
47. A method of treating a disease or disorder comprising administering to a subject in need thereof the host cell of claim 37.
48. A pharmaceutical composition or medicament comprising the polypeptide in any one of claims 1 to 13, and a pharmaceutically acceptable carrier, diluent or excipient.
49. Use of the pharmaceutical composition or medicament of claim 48, wherein said use is for the treatment of a disease or disorder.
50. Use of the pharmaceutical composition or the medicament according to claim 49, wherein the disease or disorder is cancer.
51. An Pseudomonas exotoxin A (PE-A) polypeptide, wherein said polypeptide comprises a mutation at a position corresponding to amino acid position 184 in SEQ ID NO:l (or position 196 in SEQ ID NO:2) wherein an isoleucine at position 184 (or position 196 in SEQ ID NO:2) is substituted with a different amino acid.
52. The polypeptide of claim 51 , wherein said polypeptide does not have have PE-A biological activity.
53. A method for assaying the immunogemcity of a mutated form of Pseudomonas exotoxin A (PE-A), wherein said method comprises:
(a) contacting immune cells with a mutated form of PE-A; and
(b) assaying immune cell stimulation,
wherein said mutated form of PE-A comprises a mutation at a position corresponding to amino acid position 184 in SEQ ID NO:l (or position 196 in SEQ ID NO:2) wherein an isoleucine at position 184 (or position 196 in SEQ ID NO:2) is substituted with a different amino acid, and wherein said mutated form of PE-A also comprises one or more additional amino acid substitutions compared to a wild-type form of PE-A.
54. The method of claim 53, wherein said immune cells are human immune cells.
55. The method of claim 54, wherein said immune cells are human T-cells, cells of a human T-cell lineage, human B-cells, or cells of a human B-cell lineage.
56. A polynucleotide encoding the fusion protein of claim 24,
57. An expression vector comprising the polynucleotide of claim 56.
58. A host cell comprising the expression vector of claim 57.
59. A method of producing the fusion protein of claim 24, wherein said method comprises:
a) obtaining a host cell comprising a polynucleotide encoding said
polypeptide or fusion protein;
b) exposing said host cell to conditions wherein said fusion protein is produced.
A method of producing the fusion protein of claim 24, wherein said method comprises use of an expression system comprising;
a) a first polynucleotide encoding a first hybrid polypeptide
comprising:
(i) a first ligand binding domain; and
(ii) a DNA-binding domain;
b) a second polynucleotide encoding a second hybrid polypeptide comprising:
(i) a second ligand binding domain; and
(ii) a transactivation domain;
c) a third polynucleotide encoding the fusion protein of claim 24, wherein said third polynucleotide is operably associated with a response element capable of being bound by the DNA-binding domain of said first hybrid polypeptide;
wherein the first ligand binding domain and the second ligand binding domain are capable of ligand-induced dimerization,
wherein expression of the fusion protein of claim 24 is modulated by a ligand which induces dimerization of said first and said second ligand binding domains, wherein the fusion protein of claim 24 is produced by allowing said ligand to contact said first and said second ligand binding domains.
A single expression vector or two or more expression vectors comprising:
a) a first polynucleotide encoding a first hybrid polypeptide
comprising:
(i) a first ligand binding domain; and
(ii) a DNA-binding domain;
b) a second polynucleotide encoding a second hybrid polypeptide comprising:
(i) a second ligand binding domain; and
(ii) a transactivation domain;
c) a third polynucleotide encoding the fusion protein of claim 24, wherein said third polynucleotide is operably associated with a response element capable of being bound by the DNA-binding domain of said first hybrid polypeptide.
62. The expression vector or expression vectors of claim 61 , wherein one or more of the vectors is a viral expression vector.
63. A host cell comprising the expression vector or expression vectors of claims 62.
64. A method of treating a disease or disorder comprising administering to a subject in need thereof the fusion protein of claim 24.
65. A method of treating a disease or disorder comprising administering to a subject in need thereof the polynucleotide of claim 35.
66. A method of treating a disease or disorder comprising administering to a subject in need thereof the expression vector of claim 36.
67. A method of treating a disease or disorder comprising administering to a subject in need thereof the host cell of claim 37,
68. A pharmaceutical composition or medicament comprising the fusion protein of claim 24, and a pharmaceutically acceptable carrier, diluent or excipient.
69. A pharmaceutical composition or medicament comprising the polynucleotide of claim 35, and a pharmaceutically acceptable carrier, diluent or excipient,
70. A pharmaceutical composition or medicament comprising the expression vector of claim 36, and a pharmaceutically acceptable carrier, diluent or excipient.
71. A pharmaceutical composition or medicament comprising the host cell of claim 37, and a pharmaceutically acceptable carrier, diluent or excipient.
72. The method of claim 38, wherein said polypeptide is a fusion protein.
73. The method of claim 39, wherein said polypeptide is a fusion protein.
Applications Claiming Priority (4)
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|---|---|---|---|
| US201161531576P | 2011-09-06 | 2011-09-06 | |
| US61/531,576 | 2011-09-06 | ||
| US13/604,173 US8932586B2 (en) | 2011-09-06 | 2012-09-05 | Modified forms of Pseudomonas exotoxin A |
| US13/604,173 | 2012-09-05 |
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| Publication Number | Publication Date |
|---|---|
| WO2013036574A1 WO2013036574A1 (en) | 2013-03-14 |
| WO2013036574A4 true WO2013036574A4 (en) | 2013-06-13 |
Family
ID=47832534
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2012/053868 WO2013036574A1 (en) | 2011-09-06 | 2012-09-06 | Modified forms of pseudomonas exotoxin a |
Country Status (3)
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|---|---|
| US (5) | US8932586B2 (en) |
| TW (1) | TW201326196A (en) |
| WO (1) | WO2013036574A1 (en) |
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-
2012
- 2012-09-05 US US13/604,173 patent/US8932586B2/en not_active Expired - Fee Related
- 2012-09-06 WO PCT/US2012/053868 patent/WO2013036574A1/en active Application Filing
- 2012-09-06 TW TW101132552A patent/TW201326196A/en unknown
-
2014
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-
2015
- 2015-12-30 US US14/984,006 patent/US9447387B2/en not_active Expired - Fee Related
-
2016
- 2016-08-11 US US15/234,297 patent/US9677055B2/en not_active Expired - Fee Related
-
2017
- 2017-04-06 US US15/481,131 patent/US20170275600A1/en not_active Abandoned
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|---|---|
| US8932586B2 (en) | 2015-01-13 |
| US20130121983A1 (en) | 2013-05-16 |
| US20170275600A1 (en) | 2017-09-28 |
| US20150291941A1 (en) | 2015-10-15 |
| TW201326196A (en) | 2013-07-01 |
| US9447387B2 (en) | 2016-09-20 |
| US9371517B2 (en) | 2016-06-21 |
| US9677055B2 (en) | 2017-06-13 |
| US20170037386A1 (en) | 2017-02-09 |
| WO2013036574A1 (en) | 2013-03-14 |
| US20160108377A1 (en) | 2016-04-21 |
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