WO2013151310A1

WO2013151310A1 - Trans-illuminator using leds

Info

Publication number: WO2013151310A1
Application number: PCT/KR2013/002735
Authority: WO
Inventors: 백계승; 이숙경
Original assignee: 주식회사 노블바이오
Priority date: 2012-04-03
Filing date: 2013-04-02
Publication date: 2013-10-10
Also published as: KR101411460B1; KR20130112343A

Abstract

The present invention relates to a trans-illuminator and, more particularly, to a trans-illuminator using LEDs, in which an LED light source for checking an electrophoresed sample piece is installed in a layer structure surrounding a circumference of a tray on which a sample is placed so that a light is emitted from a side of the tray, a light guide diffusion plate on which V-cutting is performed is installed in an inner space surrounded by the LED light source formed in the layer structure so that the light emitted from the LED light source is evenly diffused on a bottom surface along the light guide diffusion plate and is uniformly supplied on the entire surface of the tray placed in a tray insertion groove formed in a housing for checking the sample piece, and LEDs of different kinds are installed on each layer of the LED light source formed in the layer structure, so that a sample band may be clearly viewed through one device even after sample pieces of different kinds are electrophoresed.

Description

Trans illuminator using LED

The present invention relates to a trans-illuminator, and more particularly, by installing a LED light source for identifying a fragment of the electrophoretic sample in a layer structure surrounding the periphery of the tray on which the sample is placed so that the light is irradiated from the side, the layer structure In the inner space enclosed by the LED light source is provided with a light guide plate made of V-cutting, the light irradiated from the LED light source is spread evenly on the bottom along the light guide plate to be placed in the tray insertion groove formed in the housing for checking the fragments. In addition to being uniformly supplied to the entire surface of the device, different types of LEDs are installed in each layer of the LED light source in a layer structure, so that the sample band can be clearly observed with one device even after electrophoresis of different kinds of fragments. The present invention relates to a trans illuminator using an LED.

In general, electrophoresis refers to a phenomenon in which charged particles (ions) move in an electric field toward an anode or a cathode, and particles moving in an electric field move for the same time due to various factors such as the amount of charge, size, and shape. Since the distances are different, in the field of biotechnology, electrophoresis of molecules such as proteins, cells, or DNA is performed to observe sample bands generated by differences in movement distance.

In order to confirm the degree of DNA or protein transfer after electrophoresis, the gel is moved to a transilluminator equipped with a light source, and then the gel is irradiated with light to irradiate the DNA or protein. The band generated according to the degree of movement will be observed.

However, the trans-illuminator used in the related art generally uses an ultraviolet lamp as disclosed in Korean Laid-Open Patent Publication No. 2000-0001431. As described above, when the UV lamp is used, there is a problem that the experimenter needs to repeatedly expose the UV light unnecessarily, and there is a cumbersome and complicated problem such as having to separately provide a means for preventing the UV light from being exposed to the outside of the transilluminator.

Accordingly, recently, a trans-illuminator has been proposed that uses a light emitting diode (LED) as a light source to fundamentally eliminate the risk of exposure to ultraviolet rays by using a light emitting diode (LED) as disclosed in US Patent Publication No. US2006 / 0030026 A1.

However, in the case of the trans illuminator disclosed in US 2006/0030026 A1, in order to evenly irradiate light on the entire surface of the gel in which electrophoresis was performed, a plurality of LEDs had to be opened and installed at the bottom of the housing. There was a problem that the kind of samples that can be observed by one transilluminator is limited to be limited depending on the kind.

In addition, as disclosed in US 2006/0030026 A1, when a plurality of LEDs are installed inside the trans illuminator so that light is irradiated upward from the bottom direction, the overall brightness may be improved, Since the brightness of the background is too bright to identify the band indicating the degree of movement of the fragments after electrophoresis, there is a problem that causes inconvenience in observation due to the decrease in the clarity of the DNA fragment to be observed.

The problem to be solved by the present invention is to install the LED light source for identifying the fragments of the electrophoretic sample in a layer structure surrounding the circumference of the tray on which the sample is placed so that the light is irradiated from the side, By installing a light guide plate made of V-cutting in the enclosed inner space, the light emitted from the LED light source is spread evenly on the bottom along the light guide plate, and the entire surface of the tray placed in the tray insertion groove formed in the housing for checking the fragments. Transformers using LEDs that are supplied uniformly and that different types of LEDs are installed on each layer of the layered LED light source so that the sample band can be clearly observed with one device even after electrophoresis of different kinds of fragments. In providing an illuminator.

The trans illuminator using the LED for solving the above problem,

A housing in which a tray insertion groove is formed, the space in which the light source is accommodated and the electrophoretic sample to be observed is placed; A side light source unit accommodated in the housing and disposed to surround a side circumference of the tray insertion groove, the side light source unit comprising a plurality of LEDs irradiating light from the side of the tray insertion groove to the center of the tray insertion groove; A light guide plate installed in an inner space surrounded by the side light source; And a tray on which the electrophoretic sample is seated and inserted into the tray insertion groove.

In this case, the side light source unit is formed of a layer structure of different types of LEDs capable of irradiating light of different wavelengths, and thus the side light source unit forming the layer structure is installed at a lower layer to observe the electrophoretic state of the protein sample. It is characterized in that it comprises a white light LED, and a blue light LED installed on the upper layer to observe the electrophoretic state of the nucleic acid (DNA / RNA) sample.

In addition, the light guide plate, the light guide plate is made of V-cutting; A reflection plate installed under the light guide plate to reflect light emitted from the white light LED upward; And a diffuser plate installed on an upper portion of the light guide plate to diffuse the light reflected from the reflecting plate to the entire surface.

The housing may include an LED accommodating part to be installed around the tray insertion groove while the LEDs forming the side light source part form a layer structure; The LED accommodating portion has a bent portion on which the blue light LED can be seated is formed on the upper portion, a jaw supporting the upper portion of the light guide diffuser is formed in the center, and the entire cross section is formed in English to form a space in which the white light LED can be placed. It is characterized by being configured to form an F shape.

In addition, the tray,

Two support plates supporting the sides; A lower plate connecting lower portions of the support plate and made of black acrylic; The lower plate is spaced apart from each other by the support plate is characterized in that consisting of the upper plate made of transparent acrylic.

In addition, the tray,

Two support plates supporting the sides; And it may be composed of a smog plate made of acrylic or polycarbonate (PC) interconnected in the middle of the support plate is smog treatment.

The present invention is implemented by using a trans-illuminator, which was conventionally made using a UV lamp, as an LED light source, thereby making the experimental environment using a trans-illuminator equipped with essential equipment for all laboratories performing genetic engineering experiments. There is an effect that can be changed.

In addition, by using the LED light source, the band can be observed with clarity that does not fall compared to conventional UV lamps, there is an effect that can perform a convenient and stable experiment using the LED light source.

In addition, the LED light source is provided in a layer structure, and different types of LEDs are provided in each layer, so that the protein and nucleic acid (DNA / RNA) can be observed by a single trans illuminator while changing the LED to be lit by simple operation. It has an effect.

1 is an exploded perspective view of a trans illuminator using the LED according to the present invention.

Figure 2 is a perspective view of the coupling of the trans illuminator using the LED according to the present invention.

Figure 3 is a perspective view of the LED housing coupled to the housing in accordance with the present invention.

Figure 4 is a cross-sectional view of the installation of the side light source according to the present invention.

5 is a front view of a tray showing a state in which the observation target according to the present invention is placed;

Fig. 6 is a front view showing another embodiment of a tray showing a state in which an observation object according to the present invention is placed;

7 is a photograph showing a tray according to the present invention.

8 is an exemplary photograph showing a state in which the white light LED of the trans-illuminator using the LED according to the present invention is turned on.

Figure 9 is an exemplary photo showing a state in which the blue light LED of the trans-illuminator using the LED according to the present invention.

10 is an exemplary photograph showing a state in which a blue light LED is turned on by mounting a tray in a trans illuminator using an LED according to the present invention;

Hereinafter, specific embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is an exploded perspective view of a trans illuminator using an LED according to the present invention, Figure 2 is a combined perspective view of a trans illuminator using an LED according to the present invention.

1 and 2, the trans-illuminator 10 using the LED according to the present invention, the housing 100 is formed with a tray insertion groove which is a space in which the light source is accommodated and the electrophoretic sample to be observed is placed, and in the housing A side light source unit 200 including a plurality of LEDs received and disposed to surround a side circumference of the tray insertion groove, a light guide plate 300 installed in an inner space surrounded by the side light source unit, and the electrophoretic sample It is configured to include a tray 400 is seated and inserted into the tray insertion groove.

The housing 100 forms the overall external shape of the trans illuminator, and the LED receiving unit 130 is installed around the tray insertion groove 140 to install the LED in a layer structure.

At this time, one side of the tray insertion groove 140, the light source selection unit 150 made of a light switch for controlling the lighting of the side light source unit 200 is electrically connected to the side light source unit 200 is installed, It is configured to receive power for lighting the side light source unit from a supply unit (not shown).

In addition, the top plate 110 is coupled to the upper portion of the housing to prevent the inflow of foreign matter, etc., and the base plate 120 of the plate shape for fixing the housing is coupled to the lower portion of the housing. It is composed. In this case, the top plate 110 is formed with a tray insertion groove through which the tray 400 is inserted and exited, and the base plate 120 is formed as a sealed plate to support the light guide plate or the tray. It is preferable to be.

The LED accommodating part 130 is installed along the circumference of the tray insertion groove 140 in which the side light source is installed, so that the white light LED, the light guide plate, and the blue light LED form a layered structure and can be stably coupled to the housing. As shown in Fig. 3, a bent portion on which the blue light LED can be seated is formed at the upper part, a jaw supporting the upper part of the light guide diffuser is formed at the center, and an overall cross section at the lower part to form a space in which the white light LED can be placed. It is preferable to be comprised so that this alphabet letter F shape may be formed. At this time, the light guide plate is positioned inside the LED receiving unit, and thus the light guide plate, in particular, the light guide plate is coupled by the white light LED.

1 and 2 shows that the tray receiving groove 140 is configured in the form of a square bar, it is preferable that four LED receiving unit 130 is also installed to cover all four sides of the square. However, the shape of the tray accommodating groove and the shape and number of the LED accommodating portions are not limited thereto, and may be variously formed according to the type of the tray.

In addition, the light source selection unit 150 is composed of each lighting switch for controlling the lighting of each of the blue light LED and the white light LED, the lighting switch for controlling the blue light LED is configured to adjust the intensity of the light irradiated from the blue light LED. It is preferable to be. In this case, it is well known that lighting and sequential light intensity control are possible by repeatedly pressing the same button, and thus a detailed description thereof will be omitted.

The side light source unit 200 is a plurality of LEDs that are received in the LED receiving unit 130 and irradiates the light for observing the band appearing in the sample from the side of the tray insertion groove 140 to the center of the tray insertion groove, It is installed to completely surround the side circumference of the tray insertion groove 140.

In addition, the side light source unit 200 is installed in the LED storage unit 130 while forming different types of LEDs that can irradiate light of different wavelengths in a layer structure, as shown in FIG. It comprises a white light LED 210 that can observe the electrophoretic state of the sample, and a blue light LED 220 that is installed on the upper layer and can observe the electrophoretic state of the nucleic acid (DNA / RNA) sample.

Even when different types of LEDs are installed as described above, only one type of LED is turned on by the light source selection unit 150 when observing a sample. Therefore, only a different operation of a light source of a sample to be lit depends on the type of sample to be observed. Thus, proteins and nucleic acids (DNA / RNA) can be observed by a single transilluminator.

The light guide plate 300 is positioned in an inner space surrounded by the white light LED, and as shown in FIG. 4, the light guide plate 310 having V cut is attached to the bottom of the light guide plate and irradiated from the white light LED. And a diffuser plate 320 for reflecting the light upward, and a diffuser plate 330 disposed on the light guide plate to diffuse the light reflected from the reflector to the entire surface.

As such, the light guide plate 300 is surrounded by the white light LED and positioned below the tray 400 so that the light irradiated from the side surface of the tray insertion groove 140 is guided below the light guide diffuser plate. Take the 300 to the center portion of the tray insertion groove 140 and then to be irradiated toward the top, uniform brightness from the bottom of the tray 400 toward the top to the center as well as the side of the sample to observe Light can be irradiated.

As such, the light irradiated from the side by the light guide plate 300 coupled with the V-cut light guide plate, the reflector plate, and the diffuser plate is not dependent on the direct position of the LED light source irradiated with the gel to be observed. By allowing to be uniformly irradiated from above, the size of the tray 400, that is, the size of the gel 20 subjected to electrophoresis can be enlarged.

As shown in FIGS. 5 and 7, the tray 400 includes two support plates 410 for supporting side surfaces, a lower plate 420 connecting black parts of the support plates, and made of black acrylic. The upper plate 430 is spaced apart from the lower plate and connected to the support plates and made of transparent acrylic.

At this time, the upper plate 430 is installed at a lower side spaced apart from the top of the support plate 410 by both sides are configured to be blocked by the upper end of the support plate 410, so that the sample placed on the upper plate falls to the outside It is desirable to be configured so that it can be safely prevented.

As described above, the lower plate 420 is formed as a plate of black background to overcome the phenomenon that the sample of the gel to be observed is blurred when the light source is irradiated to maximize the sharpness of the analysis sample. As such, when the lower plate 420 is composed of black acrylic, it becomes suitable to observe the electrophoretic gel of nucleic acid (DNA / RNA).

In addition, as another embodiment of the tray 400, the tray for observing the electrophoretic gel of the protein, as shown in Figure 6, two support plates 410 for supporting the side, in the middle of the support plate It is composed of a smog plate 440 made of acrylic or polycarbonate (PC) interconnected and smog-treated. Accordingly, in another embodiment of the tray, the protein electrophoretic gel placed on the tray excluding the lower plate is emitted from the white light LED, and then uniformly diffused light is emitted from the light guide plate so that the band can be observed.

Next, the operation of the trans illuminator using the LED according to the present invention configured as described above will be described.

8 is an exemplary photograph showing a state in which the white light LED of the trans illuminator using the LED according to the present invention, Figure 9 is an exemplary photograph showing a state in which the blue light LED of the trans illuminator using the LED according to the present invention is lit, 10 is an exemplary photograph showing a state in which a blue light LED is turned on by mounting a tray in a trans illuminator using an LED according to the present invention.

First, FIG. 8 illustrates that white light for protein observation is turned on. By pressing a switch of the light source selection unit 150 provided in the housing, the white light LED 210 installed in the lower layer of the side light source unit installed in the layer structure is turned on.

At this time, as can be seen in Figures 8 (a) and 8 (b), the white light LED 210 is installed to surround the four side of the tray insertion groove 140, bar of the tray insertion groove 140 Light is emitted from four sides.

However, since the white light LED 210 is located under the tray 400, the light emitted from the white light LED 210 is irradiated to the bottom surface of the white light LED 210 is not directly irradiated to the tray or gel. As such, the light irradiated toward the bottom of the tray or gel is uniformly spread from the bottom of the tray 400 along the entire surface of the light guide plate 300, and then irradiates light onto the gel on the top of the tray 400.

As such, the light generated from the white light LED 210 is uniformly spread from the bottom of the tray 400 to the entire surface of the light guide plate 300 and then irradiated to the upper part where the observation object is located, so that the area adjacent to the light source is bright but far from the light source. The upper part of the center is prevented from being darkened so that light can be uniformly radiated over the entire surface of the gel to be observed.

In addition, Figure 9 shows that the blue light is turned on for nucleic acid (DNA / RNA) observation, blue light LED (220) installed on the upper layer of the side light source unit installed in a layer structure by pressing the switch of the light source selection unit 150 provided in the housing ) Will light up.

At this time, as can be seen in Figures 9 (a) and 9 (b), the blue light LED 220 is also installed to surround the four side of the tray insertion groove 140, bar tray insertion groove 140 Light is emitted from four sides.

However, since the gel subjected to the nucleic acid (DNA / RNA) electrophoresis to be observed is placed on the upper plate 430 of the tray, the light irradiated from the blue light LED 220 is not irradiated directly to the gel but irradiated to the bottom thereof. The light irradiated to the lower part of the gel to be observed as described above is uniformly spread from the bottom of the gel along the entire surface of the light guide plate 300, as can be seen in FIGS. 10 (a) and 10 (b), Since the light is irradiated to the tray 400 and the gel placed on the upper part, the area adjacent to the light source is bright but the center far from the light source is prevented from being dark to uniformly irradiate the light over the entire surface of the gel to be observed. It becomes possible.

In this way, the LED is used as a light source for irradiating light from the side, and the light is irradiated from the lower part of the gel to the upper part after uniformity over the entire surface of the tray through the light guide plate without directly irradiating the gel to be observed. By doing so, it is possible to prevent the specific area adjacent to the light source from being too bright and the other areas from being dark, thereby improving the convenience of sample viewing.

In addition, although the light source to be irradiated differs depending on the object to be observed, it is different from the gel only by selectively lighting the blue light LED and the white light LED installed in a layer structure by pressing each light switch constituting the light source selection unit. Since the light source can be irradiated uniformly, both a protein and a nucleic acid (DNA / RNA) can be observed using a single device.

In the above description, the technical idea of the present invention has been described with the accompanying drawings. However, the present invention has been described by way of example and is not intended to limit the present invention. It is obvious that any person having ordinary knowledge in the technical field to which the present invention belongs can make various modifications and imitations without departing from the scope of the technical idea of the present invention.

Claims

A housing having an opening,

A plurality of light sources installed on an inner side of the housing,

A diffusion plate accommodated in the housing to diffuse the light emitted from the plurality of light sources;

A trans illuminator comprising a support plate detachably coupled to the opening and adapted to support a sample,

The support plate,

A support surface adapted to support the sample, and

A side located at a lower edge of the support surface,

The side surface includes a through hole and is adapted to pass the light through the through hole.

Trans illuminator.
The method of claim 1,

The support plate,

Another side located at an upper edge of the support surface, and

It is connected to the other side, and further comprising a locking jaw seated on the upper surface of the housing

Trans illuminator.
The method of claim 2,

Width of the locking jaw is 4mm to 10mm

Trans illuminator.
The method of claim 2,

The locking jaw includes a plurality of corners, and one or more corners of the plurality of corners are formed in a round shape.

Trans illuminator.
The method of claim 2,

The support plate is connected to the locking jaw is formed in a direction parallel to the other side, and comprises a pair of removable holding parts spaced apart facing each other

Trans illuminator.
The method of claim 5,

The length in the first direction of the housing parallel to one edge of the genital housing is greater than the length in the second direction of the housing perpendicularly intersecting the first direction,

The pair of detachable gripping portions may face each other along the first direction.

Trans illuminator.
The method of claim 6,

One or more grip portions of the pair of detachable grip portions extend in the second direction.

Trans illuminator.
The method of claim 1,

The plurality of light sources are arranged to be spaced apart from each other in one direction, and the through hole is formed to extend in length corresponding to the plurality of light sources.

Trans illuminator.
The method of claim 8,

The plurality of light sources,

A plurality of white light sources, and

A plurality of blue light sources spaced apart from the plurality of white light sources and positioned above the plurality of white light sources;

Trans illuminator.
The method of claim 1,

The opening includes a plurality of corners, wherein at least one of the plurality of corners is rounded.

Trans illuminator.
The method of claim 1,

The support plate further includes another support surface spaced apart from the support surface by the side surface and adapted to be received inside the housing.

Trans illuminator.
The method of claim 1,

The area of the first support surface is smaller than the area of the opening

Trans illuminator.