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WO2018171010A1 - Upper-cover type fungus culture device - Google Patents

Upper-cover type fungus culture device Download PDF

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Publication number
WO2018171010A1
WO2018171010A1 PCT/CN2017/083399 CN2017083399W WO2018171010A1 WO 2018171010 A1 WO2018171010 A1 WO 2018171010A1 CN 2017083399 W CN2017083399 W CN 2017083399W WO 2018171010 A1 WO2018171010 A1 WO 2018171010A1
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WIPO (PCT)
Prior art keywords
culture
culture dish
overcapped
fungus
slide
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PCT/CN2017/083399
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French (fr)
Chinese (zh)
Inventor
于津
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhuhai Meihua Medical Technology Ltd
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Zhuhai Meihua Medical Technology Ltd
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Publication of WO2018171010A1 publication Critical patent/WO2018171010A1/en
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  • the present invention relates to a microbial experimental device, and more particularly to a supercapped fungal culture device.
  • the present invention provides a capped fungal culture device which can observe the growth of fungal culture under an oil microscope and a high power microscope, and reduces the use cost.
  • a capped fungus culture device comprising a slide glass (1) and a culture dish (2), characterized in that the slide glass (1) is disposed at Below the culture dish (2), the slide (1) and the culture dish (2) are integrally formed and form a fungal culture chamber (3), wherein the slide (1) serves as a chassis for the culture dish (2).
  • the upper portion of the culture chamber (3) is provided with an upper cover (5), the upper cover (5) is a glass piece, and the side wall of the culture dish is provided with a through hole (4) for extending into the inoculation, The through hole (4) is in communication with the fungal culture chamber (3).
  • the axial direction of the through hole is perpendicular to the longitudinal direction of the slide glass.
  • the petri dish has a circular cross section.
  • the cover glass has a circular shape and a diameter larger or smaller than the diameter of the culture dish.
  • the cover glass is ultrasonically welded to the upper portion of the culture dish.
  • the slide glass is provided with a frosted area on both sides.
  • the slide glass and the culture dish are each made of a light transmissive polystyrene material.
  • the slide has a length of 50 to 100 mm and a width of 20 to 30 mm.
  • the four corners of the slide have a chamfer.
  • the slide has a length of 50 to 100 mm and a width of 20 to 30 mm.
  • the culture dish has a diameter of 15 to 25 mm and a height of 2 to 5 mm, and the through hole has an inner diameter of 1.0 to 4.0 mm.
  • one side of the slide glass is further provided with a trademark area marked with a trademark.
  • the present invention provides an overcapped fungal culture apparatus which utilizes a glass sheet or other material having a thinner thickness by modifying the upper lid of the culture dish.
  • the microscope is made thinner, so that the microscope is closer to the fungus for observation, so that the morphology of the fungus can be observed more clearly under the same multiple microscope, thereby achieving the use of a low power microscope.
  • the cost of purchasing a high power microscope is reduced, and significant economic benefits are obtained.
  • Figure 1 is a schematic structural view of an embodiment of the present invention
  • Figure 2 is a side plan view of an embodiment of the present invention.
  • Figure 3 is a rear elevational view of Figure 2, in accordance with one embodiment of the present invention.
  • Figure 4 is a rear elevational view of Figure 2 of another embodiment of the present invention.
  • the top cover fungus culture apparatus comprises a slide glass 1 on the front side of which a culture dish 2 is disposed, the slide glass 1 and the culture dish 2 are integrally formed, and the slide glass 1 is located under the culture dish 2 As its chassis, both the slide 1 and the petri dish 2 are made of a light transmissive polystyrene material.
  • the side wall of the culture dish 2 is provided with a through hole 4 for extending into the inoculation, wherein the axial direction of the through hole 4 is perpendicular to the longitudinal direction of the slide glass 1, and the through hole 4 is used for inoculating the needle to advance the fungus into the needle. Inoculation in a solid medium, the substance in the fungal culture chamber 3 shown in Fig. 2 is not volatile due to the small pore size.
  • the overcapped fungal culture apparatus of the present invention further includes an upper cover 5 made of glass and ultrasonically welded to the top end of the side wall of the culture dish 2.
  • the upper cover 5 can be replaced with a cover glass, and the side wall of the culture dish 2 can be sealed with paraffin wax to meet different requirements, and is also closer to the conventional microscopic observation mode.
  • the integrally formed slide 1 and the culture dish 2 form a fungal culture chamber 3, the through hole 4 is in communication with the fungal culture chamber 3, and both sides of the slide 1 are provided with a frosted area 7 for easy marking. Further, one side of the slide glass 1 is provided with a trademark area 8 for marking a trademark, the size and position of which are determined as needed.
  • the outer diameter of the upper cover 5 is smaller than the outer diameter of the culture dish 2 and larger than the inner diameter of the culture dish 2 to ultrasonically weld the upper cover 5 to the culture dish 2.
  • the weld bead is located on the upper annulus of the culture dish 2, and the joint 6 of the culture dish 2 and the upper cover 5 is sealed with paraffin.
  • the outer diameter of the upper cover 5 is larger than the outer diameter of the culture dish 2, so that the upper cover 5 is ultrasonically welded to the upper ring surface of the culture dish 2, and is welded.
  • the slit is located at the joint 6 of the outer edge of the torus of the petri dish 2 and the upper cover 5.
  • the cross section of the culture dish 2 may be a circular or elliptical shape or a regular polygon.
  • the culture dish 2 is circular in cross section
  • the slide glass 1 is rectangular, wherein the length of the slide 1 is It is 50 ⁇ 100mm, the width is 20 ⁇ 30mm, the diameter of the culture dish 2 is 15 ⁇ 25mm, the height is 2 ⁇ 5mm, the inner diameter of the through hole is 1.0 ⁇ 4.0mm, specifically, in this embodiment, the slide 1
  • the length of the dish is 75.9mm, the width is 25.2mm, the diameter of the culture dish 2 is 23.1mm, the height is 4.35mm, and the inner diameter of the through hole 4 is 2.7mm, so that the culture device can be placed under the microscope, and no additional fungus is needed. Place under the microscope to prevent laboratory contamination.
  • the present invention provides an overcapped fungal culture apparatus which utilizes a glass sheet or other material having a thinner thickness by modifying the upper lid of the culture dish.
  • the microscope is made thinner, so that the microscope is closer to the fungus for observation, so that the morphology of the fungus can be observed more clearly under the same multiple microscope, thereby achieving the use of a low power microscope.
  • the cost of purchasing a high power microscope is reduced, and significant economic benefits are obtained.

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Abstract

An upper-cover type fungus culture device, comprising a glass slide (1) and a culture dish (2), wherein the glass slide (1) is disposed below the culture dish (2); the glass slide (1) and the culture dish (2) are of an integrated structure and form a fungus culture chamber (3); the glass slide (1) serves as a bottom tray of the culture dish (2); an upper cover (5) is provided at the upper part of the culture chamber (3); the upper cover (5) is a glass sheet; the sidewall of the culture dish is provided with a through hole (4) for entering and inoculating; and the through hole (4) is communicated with the fungus culture chamber (3).

Description

一种上盖式真菌培养装置  Capped fungus culture device

技术领域Technical field

本发明涉及微生物实验装置,尤其涉及一种上盖式真菌培养装置。The present invention relates to a microbial experimental device, and more particularly to a supercapped fungal culture device.

背景技术Background technique

在真菌实验中大都以小培养法来观察其形态变化,真菌一般放在培养皿中的固态培养基中进行培养,培养一定时间后将真菌培养装置转移至显微镜下进行形态学鉴定,以便观察了解真菌的培养生长情况。但是在真菌培养装置放在显微镜下进行观察时,由于培养装置的上盖厚度和材料的关系,使观察真菌的清晰度受到影响,因此为了更清晰的观察真菌,不得不使用高倍数的显微镜,才能清晰地观察到真菌的形态,这样就增加了使用成本。因此市场上迫切需要一种装置来克服这些缺陷,进而降低使用成本。In the fungal experiment, most of the morphological changes were observed by small culture method. The fungi were usually cultured in a solid medium in a culture dish. After a certain period of time, the fungal culture device was transferred to a microscope for morphological identification to observe and understand. The growth and culture of fungi. However, when the fungal culture device is observed under a microscope, the clarity of the observed fungus is affected by the relationship between the thickness of the upper cover of the culture device and the material, so in order to observe the fungus more clearly, a high-magnification microscope has to be used. The morphology of the fungus can be clearly observed, which increases the cost of use. Therefore, there is an urgent need in the market for a device to overcome these drawbacks, thereby reducing the cost of use.

发明内容Summary of the invention

为了克服上述现有技术的不足,本发明提供了一种上盖式真菌培养装置,其可以在油镜和高倍显微镜下观察真菌培养生长情况,降低了使用成本。In order to overcome the above deficiencies of the prior art, the present invention provides a capped fungal culture device which can observe the growth of fungal culture under an oil microscope and a high power microscope, and reduces the use cost.

本发明解决其技术问题所采用的技术方案为:一种上盖式真菌培养装置,包括载玻片(1)和培养皿(2),其特征在于:所述载玻片(1)设置在培养皿(2)下方,所述载玻片(1)和培养皿(2)为一体成型结构并形成真菌培养腔(3),其中载玻片(1)作为培养皿(2)的底盘,所述培养腔(3)上部设置有上盖(5),所述上盖(5)为一玻璃片,所述培养皿的侧壁设置有用于伸入接种的通孔(4),所述通孔(4)与真菌培养腔(3)连通。The technical solution adopted by the present invention to solve the technical problem thereof is: a capped fungus culture device comprising a slide glass (1) and a culture dish (2), characterized in that the slide glass (1) is disposed at Below the culture dish (2), the slide (1) and the culture dish (2) are integrally formed and form a fungal culture chamber (3), wherein the slide (1) serves as a chassis for the culture dish (2). The upper portion of the culture chamber (3) is provided with an upper cover (5), the upper cover (5) is a glass piece, and the side wall of the culture dish is provided with a through hole (4) for extending into the inoculation, The through hole (4) is in communication with the fungal culture chamber (3).

作为上述技术方案的一种改进,所述通孔的轴向方向与载玻片的长度方向相垂直。As a modification of the above technical solution, the axial direction of the through hole is perpendicular to the longitudinal direction of the slide glass.

作为上述技术方案的一种改进,所述培养皿的横截面为圆形。As an improvement of the above technical solution, the petri dish has a circular cross section.

作为上述技术方案的一种改进,所述盖玻片的形状为圆形,其直径大于或小于培养皿的直径。As a modification of the above technical solution, the cover glass has a circular shape and a diameter larger or smaller than the diameter of the culture dish.

作为上述技术方案的一种改进,所述盖玻片通过超声波焊接在培养皿的上部。As a modification of the above technical solution, the cover glass is ultrasonically welded to the upper portion of the culture dish.

作为上述技术方案的一种改进,所述载玻片两侧均设有磨砂区。As an improvement of the above technical solution, the slide glass is provided with a frosted area on both sides.

作为上述技术方案的一种改进,所述载玻片和培养皿均由透光的聚苯乙烯材料制成。As a modification of the above technical solution, the slide glass and the culture dish are each made of a light transmissive polystyrene material.

作为上述技术方案的一种改进,所述载玻片的长度为50~100mm,宽度为20~30mm。As an improvement of the above technical solution, the slide has a length of 50 to 100 mm and a width of 20 to 30 mm.

作为上述技术方案的一种改进,所述载玻片的四个角均具有倒角。As an improvement of the above technical solution, the four corners of the slide have a chamfer.

作为上述技术方案的一种改进,所述载玻片的长度为50~100mm,宽度为20~30mm。As an improvement of the above technical solution, the slide has a length of 50 to 100 mm and a width of 20 to 30 mm.

作为上述技术方案的一种改进,所述培养皿的直径为15~25mm,高度为2~5mm,所述通孔的内径为1.0~4.0mm。As an improvement of the above technical solution, the culture dish has a diameter of 15 to 25 mm and a height of 2 to 5 mm, and the through hole has an inner diameter of 1.0 to 4.0 mm.

作为上述技术方案的一种改进,所述载玻片的一侧还设有标记商标的商标区域。As an improvement of the above technical solution, one side of the slide glass is further provided with a trademark area marked with a trademark.

与现有技术相比,本发明提供了一种上盖式真菌培养装置,其通过改进培养皿的上盖,使其采用具有更薄厚度的玻璃片或其它材料。在将培养皿放在显微镜下进行观察时,由于玻璃片更薄,使得显微镜更加贴近真菌进行观察,从而在同样倍数的显微镜下,能更清晰地观察真菌的形态,从而达到了使用低倍显微镜来代替高倍显微镜进行观察的效果,进而减少了采购高倍显微镜的成本,具有显著的经济效益。In contrast to the prior art, the present invention provides an overcapped fungal culture apparatus which utilizes a glass sheet or other material having a thinner thickness by modifying the upper lid of the culture dish. When the culture dish is placed under a microscope, the microscope is made thinner, so that the microscope is closer to the fungus for observation, so that the morphology of the fungus can be observed more clearly under the same multiple microscope, thereby achieving the use of a low power microscope. Instead of observing the effect of a high power microscope, the cost of purchasing a high power microscope is reduced, and significant economic benefits are obtained.

附图说明DRAWINGS

图1是本发明一个实施例的结构示意图;Figure 1 is a schematic structural view of an embodiment of the present invention;

图2是本发明一个实施例的侧俯视图;Figure 2 is a side plan view of an embodiment of the present invention;

图3是本发明一个实施例的图2的后视图;Figure 3 is a rear elevational view of Figure 2, in accordance with one embodiment of the present invention;

图4是本发明另一个实施例的图2的后视图。Figure 4 is a rear elevational view of Figure 2 of another embodiment of the present invention.

具体实施方式detailed description

如图1所示 ,根据本发明的上盖式真菌培养装置包括载玻片1,载玻片1正面设有培养皿2,载玻片1和培养皿2为一体成型结构而且载玻片1位于培养皿2下方作为其底盘,载玻片1和培养皿2均由透光的聚苯乙烯材料制成。As shown in Figure 1 The top cover fungus culture apparatus according to the present invention comprises a slide glass 1 on the front side of which a culture dish 2 is disposed, the slide glass 1 and the culture dish 2 are integrally formed, and the slide glass 1 is located under the culture dish 2 As its chassis, both the slide 1 and the petri dish 2 are made of a light transmissive polystyrene material.

此外,培养皿2的侧壁设置有用于伸入接种的通孔4,其中,通孔4的轴向与载玻片1的长度方向相垂直,通孔4用于接种针先期将真菌伸入固态培养基内接种,由于孔径较小,所以在如图2所示的真菌培养腔3内的物质不易挥发。本发明的上盖式真菌培养装置还包括上盖5,上盖5由玻璃制成,并通过超声波焊接在培养皿2的侧壁顶端上。此外,还可将上盖5替换成盖玻片,使用石蜡对培养皿2的侧壁密封处理,以满足不同的要求,也更接近于传统的显微观察模式。Further, the side wall of the culture dish 2 is provided with a through hole 4 for extending into the inoculation, wherein the axial direction of the through hole 4 is perpendicular to the longitudinal direction of the slide glass 1, and the through hole 4 is used for inoculating the needle to advance the fungus into the needle. Inoculation in a solid medium, the substance in the fungal culture chamber 3 shown in Fig. 2 is not volatile due to the small pore size. The overcapped fungal culture apparatus of the present invention further includes an upper cover 5 made of glass and ultrasonically welded to the top end of the side wall of the culture dish 2. In addition, the upper cover 5 can be replaced with a cover glass, and the side wall of the culture dish 2 can be sealed with paraffin wax to meet different requirements, and is also closer to the conventional microscopic observation mode.

进一步参见图2,一体成型的载玻片1与培养皿2形成真菌培养腔3,通孔4与真菌培养腔3连通,载玻片1两侧均设有磨砂区7,便于标记。并且载玻片1的一侧还设置有用于标记商标的商标区域8,其区域大小及位置根据需要而定。Referring further to Figure 2, the integrally formed slide 1 and the culture dish 2 form a fungal culture chamber 3, the through hole 4 is in communication with the fungal culture chamber 3, and both sides of the slide 1 are provided with a frosted area 7 for easy marking. Further, one side of the slide glass 1 is provided with a trademark area 8 for marking a trademark, the size and position of which are determined as needed.

如图3所示,根据本发明的一个优选实施例,上盖5的外径小于培养皿2的外径并且大于培养皿2的内径,以将上盖5通过超声波焊接在培养皿2的上环面上,并且焊缝位于培养皿2的上环面上,并用石蜡对培养皿2与上盖5的接合处6进行密封。As shown in FIG. 3, according to a preferred embodiment of the present invention, the outer diameter of the upper cover 5 is smaller than the outer diameter of the culture dish 2 and larger than the inner diameter of the culture dish 2 to ultrasonically weld the upper cover 5 to the culture dish 2. On the annulus, and the weld bead is located on the upper annulus of the culture dish 2, and the joint 6 of the culture dish 2 and the upper cover 5 is sealed with paraffin.

如图4所示,根据本发明的另一个优选实施例,上盖5的外径大于培养皿2的外径,以使上盖5通过超声波焊接在培养皿2的上环面上,而焊缝位于培养皿2的环面外缘与上盖5的接合处6。As shown in FIG. 4, according to another preferred embodiment of the present invention, the outer diameter of the upper cover 5 is larger than the outer diameter of the culture dish 2, so that the upper cover 5 is ultrasonically welded to the upper ring surface of the culture dish 2, and is welded. The slit is located at the joint 6 of the outer edge of the torus of the petri dish 2 and the upper cover 5.

另外,培养皿2的横截面可为圆形或椭圆形或正多边形,在此实施例中,培养皿2为横截面为圆形,载玻片1为长方形,其中,载玻片1的长度为50~100mm,宽度为20~30mm,培养皿2的直径为15~25mm,高度为2~5mm,通孔的内径为1.0~4.0mm,具体地,在此实施例中,载玻片1的长度为75.9mm,宽度为25.2mm,培养皿2的直径为23.1mm,高度为4.35mm,通孔4的内径为2.7mm,这样方便本培养装置放置在显微镜下观察,无需再额外取出真菌放置在显微镜下,防止实验室污染。In addition, the cross section of the culture dish 2 may be a circular or elliptical shape or a regular polygon. In this embodiment, the culture dish 2 is circular in cross section, and the slide glass 1 is rectangular, wherein the length of the slide 1 is It is 50~100mm, the width is 20~30mm, the diameter of the culture dish 2 is 15~25mm, the height is 2~5mm, the inner diameter of the through hole is 1.0~4.0mm, specifically, in this embodiment, the slide 1 The length of the dish is 75.9mm, the width is 25.2mm, the diameter of the culture dish 2 is 23.1mm, the height is 4.35mm, and the inner diameter of the through hole 4 is 2.7mm, so that the culture device can be placed under the microscope, and no additional fungus is needed. Place under the microscope to prevent laboratory contamination.

与现有技术相比,本发明提供了一种上盖式真菌培养装置,其通过改进培养皿的上盖,使其采用具有更薄厚度的玻璃片或其它材料。在将培养皿放在显微镜下进行观察时,由于玻璃片更薄,使得显微镜更加贴近真菌进行观察,从而在同样倍数的显微镜下,能更清晰地观察真菌的形态,从而达到了使用低倍显微镜来代替高倍显微镜进行观察的效果,进而减少了采购高倍显微镜的成本,具有显著的经济效益。In contrast to the prior art, the present invention provides an overcapped fungal culture apparatus which utilizes a glass sheet or other material having a thinner thickness by modifying the upper lid of the culture dish. When the culture dish is placed under a microscope, the microscope is made thinner, so that the microscope is closer to the fungus for observation, so that the morphology of the fungus can be observed more clearly under the same multiple microscope, thereby achieving the use of a low power microscope. Instead of observing the effect of a high power microscope, the cost of purchasing a high power microscope is reduced, and significant economic benefits are obtained.

以上所述,只是本发明的较佳实施例而已,本发明并不局限于上述实施方式,只要其以相同的手段达到本发明的技术效果,都应属于本发明的保护范围。在本发明的保护范围内其技术方案和/或实施方式可以有各种不同的修改和变化。The above is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiment, and as long as it achieves the technical effects of the present invention by the same means, it should fall within the scope of protection of the present invention. Various modifications and changes may be made to the technical solutions and/or embodiments within the scope of the invention.

Claims (10)

一种上盖式真菌培养装置,包括载玻片(1)和培养皿(2),其特征在于:所述载玻片(1)设置在培养皿(2)下方,所述载玻片(1)和培养皿(2)为一体成型结构并形成真菌培养腔(3),其中载玻片(1)作为培养皿(2)的底盘,所述培养腔(3)上部设置有上盖(5),所述上盖(5)为一玻璃片,所述培养皿的侧壁设置有用于伸入接种的通孔(4),所述通孔(4)与真菌培养腔(3)连通。 A capped fungus culture device comprising a slide (1) and a culture dish (2), characterized in that the slide (1) is disposed under the culture dish (2), the slide ( 1) and the culture dish (2) is an integrally formed structure and forms a fungal culture chamber (3), wherein the slide glass (1) serves as a chassis of the culture dish (2), and the upper portion of the culture chamber (3) is provided with an upper cover ( 5), the upper cover (5) is a glass piece, and the side wall of the culture dish is provided with a through hole (4) for extending into the inoculation, and the through hole (4) is connected with the fungal culture chamber (3) . 根据权利要求1所述的上盖式真菌培养装置,其特征在于:所述通孔(4)的轴向方向与载玻片(1)的长度方向相垂直。The overcapped fungus culture apparatus according to claim 1, wherein the axial direction of the through hole (4) is perpendicular to the longitudinal direction of the slide glass (1). 根据权利要求1所述的上盖式真菌培养装置,其特征在于:所述培养皿(2)的横截面为圆形。The overcapped fungus culture apparatus according to claim 1, wherein the culture dish (2) has a circular cross section. 根据权利要求1所述的上盖式真菌培养装置,其特征在于:所述上盖(5)的形状为圆形,其直径大于或小于培养皿(2)的外径。The overcapped fungus culture apparatus according to claim 1, wherein the upper cover (5) has a circular shape and a diameter larger or smaller than an outer diameter of the culture dish (2). 根据权利要求1或4所述的上盖式真菌培养装置,其特征在于:所述上盖(5)通过超声波焊接在培养皿(2)的上部。The overcapped fungus culture apparatus according to claim 1 or 4, characterized in that the upper cover (5) is ultrasonically welded to the upper portion of the culture dish (2). 根据权利要求1所述的上盖式真菌培养装置,其特征在于:所述载玻片(1)两侧均设有磨砂区(7)。The overcapped fungus culture apparatus according to claim 1, characterized in that the slide (1) is provided with a frosted zone (7) on both sides. 根据权利要求1所述的上盖式真菌培养装置,其特征在于:所述载玻片(1)和培养皿(2)均由透光的聚苯乙烯材料制成。The overcapped fungal culture apparatus according to claim 1, wherein the slide glass (1) and the culture dish (2) are each made of a light transmissive polystyrene material. 根据权利要求7所述的上盖式真菌培养装置,其特征在于:所述载玻片(1)的长度为50~100mm,宽度为20~30mm。The overcapped fungus culture apparatus according to claim 7, wherein the slide glass (1) has a length of 50 to 100 mm and a width of 20 to 30 mm. 根据权利要求7所述的上盖式真菌培养装置,其特征在于:所述载玻片(1)的四个角均具有倒角。The overcapped fungus culture apparatus according to claim 7, wherein the four corners of the slide glass (1) are chamfered. 根据权利要求1所述的上盖式真菌培养装置,其特征在于:所述载玻片(1)的一侧设有标记商标的商标区域(8)。The overcapped fungus culture apparatus according to claim 1, wherein one side of the slide glass (1) is provided with a trademarked trademark area (8).
PCT/CN2017/083399 2017-03-22 2017-05-08 Upper-cover type fungus culture device Ceased WO2018171010A1 (en)

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CN201720284583.4 2017-03-22

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CN201915102U (en) * 2011-01-26 2011-08-03 于莉 Cell culture slide
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CN205099668U (en) * 2015-10-30 2016-03-23 陈鹏 A culture apparatus for observing fungi form
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201427969Y (en) * 2009-06-23 2010-03-24 王金柱 Novel cell culture glass sheet
CN201737930U (en) * 2010-07-15 2011-02-09 中国人民解放军第四军医大学 Small device for culturing funguses
CN201915102U (en) * 2011-01-26 2011-08-03 于莉 Cell culture slide
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