[go: up one dir, main page]

WO2018186657A1 - Biopuce - Google Patents

Biopuce Download PDF

Info

Publication number
WO2018186657A1
WO2018186657A1 PCT/KR2018/003918 KR2018003918W WO2018186657A1 WO 2018186657 A1 WO2018186657 A1 WO 2018186657A1 KR 2018003918 W KR2018003918 W KR 2018003918W WO 2018186657 A1 WO2018186657 A1 WO 2018186657A1
Authority
WO
WIPO (PCT)
Prior art keywords
functional layer
hydrogel functional
medium substrate
binding
transducer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/003918
Other languages
English (en)
Korean (ko)
Inventor
김종성
성혁기
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scholar Foxtrot Co Ltd
Original Assignee
Scholar Foxtrot Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020180038036A external-priority patent/KR102037800B1/ko
Application filed by Scholar Foxtrot Co Ltd filed Critical Scholar Foxtrot Co Ltd
Publication of WO2018186657A1 publication Critical patent/WO2018186657A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Definitions

  • a biochip that can detect and analyze multiple bonds of a protein separately from a single bond.
  • a biochip which can detect multiple bonds of a target protein separately from a single bond.
  • the electrical characteristics may be at least one of voltage, current, and impedance.
  • the electrochemical sensor may be any one of a multiple electrode device / circuit, a complementary metal oxide semiconductor (CMOS) device / circuit, or a charge coupled device (CCD) device / circuit.
  • CMOS complementary metal oxide semiconductor
  • CCD charge coupled device
  • Biochip is provided.
  • the hydrogel functional layer may further include nanoparticles.
  • the nanoparticles may be combined with the binding medium substrate.
  • the nanoparticles may be independent of the binding medium substrate.
  • FIG. 1 is a front structural diagram of a biochip according to one embodiment.
  • FIG. 2 is a side structure diagram of a biochip according to one embodiment.
  • FIG. 3 is a schematic diagram of a biochip injected with nanoparticles according to an exemplary embodiment.
  • FIG. 4 is a schematic diagram of a biochip injected with nanoparticles according to another exemplary embodiment.
  • FIG. 5 is a diagram illustrating an example of a biochip implemented with an optical fiber.
  • FIG. 6 is a diagram illustrating another example of a biochip implemented with optical fibers.
  • 1 is a structural diagram of a biochip.
  • a biochip 100 may include a hydrogel having a binding medium substrate formed thereon and changing physical properties due to multiple binding between the administered target protein and the binding medium substrate.
  • Transducer which transmits a displacement signal corresponding to the change of the physical property of the hydrogel functional layer and the sensing area 110 in which the hydrogel functional layer is formed to an external analysis device.
  • 120 the substrate 130 supporting the transducer 120 and the sensing region 110, and the power-in and the displacement signal for providing a driving power or an input signal to the transducer 120 as external analysis equipment.
  • Interface 140 including data_out circuitry for delivery.
  • the physical properties described above may include mechanical or mechanical properties such as strength, hardness, and elongation, electrical conductivity, specific resistance, transmittance, refractive index, thermal conductivity, and the like. Thermal properties such as thermal expansion coefficient, specific heat and the like, and temperature properties such as melting point and boiling point are collectively meant.
  • the biochip 100 may transmit the displacement signal to the portable device, which is an external analysis device, through the interface 140, and the portable device may be a smartphone.
  • the external analysis device is a device that provides the user with visual, audio, and tactile results of analysis related to multiple binding of the target protein and the binding mediator.
  • the external analysis equipment refers to a device having a computing power capable of analyzing the displacement signal output from the biochip 100 to measure the amount or / and concentration of multiple bonds.
  • the biochip 100 may be designed to be removable and replaceable to various external analysis equipment.
  • the sensing region 110 and the transducer 120 may be formed in a layer structure on the substrate 130.
  • the interface 140 may be located on a portion of the substrate 130.
  • a housing 150 may be formed on the top of the biochip 100, and the housing 150 may easily target the target protein to measure the amount of multiple binding. It may have a structure specifically designed for administration.
  • a hydrogel functional layer is formed in at least a portion of the sensing region 110.
  • the sensing region 110 only means a space in which the hydrogel functional layer is formed, but does not mean a physically separated device or location in the biochip 100.
  • a binding mediator is formed which multiplexes with the target protein.
  • the hydrogel functional layer provides a resolution capability that can distinguish multiple bonds and single bonds between the target protein and the binding medium substrate administered on the biochip 100. When using the hydrogel functional layer, it is possible to distinguish between single bond and multiple bond of the target protein and the binding mediator without labeling phosphor molecules.
  • a binding mediator is formed for reaction with the target protein.
  • the binding mediator may be at least one of a receptor, a ligand, DNA, or RNA.
  • the binding mediator may be a mixture of two or more of the receptor, ligand, DNA, and RNA.
  • the binding mediator formed on the hydrogel functional layer may be specifically designed to detect multiple binding with a specific target protein.
  • the hydrogel functional layer hardly changes its physical properties when a single bond occurs between the target protein and the binding mediator.
  • the binding between the target protein and the binding media substrate is multiple bonds, the physical properties of the hydrogel functional layer change significantly due to the dewelling occurring in the hydrogel functional layer. That is, the hydrogel functional layer can be used to distinguish single bonds and multiple bonds between the target protein and the binding media substrate, and the amount of multiple bonds between the target protein and the binding media substrate can be utilized by changing the physical properties of the hydrogel functional layer. Can be detected easily.
  • the physical properties of the hydrogel functional layer may vary locally or entirely depending on the method of making the hydrogel functional layer or the reactant.
  • the hydrogel functional layer may be manufactured in a multi-channel manner. By dividing the physical area of the hydrogel functional layer into two or more, it is possible to detect a variety of multiple bonds between the target protein and the binding media in one biochip. According to one side, the area of one hydrogel functional layer may be divided into two or more, and the bonding medium substrate formed in the corresponding area may be different.
  • the hydrogel functional layer may comprise a copolymer consisting of a main monomer and a comonomer.
  • the hydrogel functional layer, the main monomer and the comonomer may be polymerized with a crosslinking agent.
  • monomers capable of forming hydrogels that are sensitive to heat, ionic strength, or pH may be used.
  • the main monomer may be selected from the group consisting of N-isopropyl acrylamide, poly (N-acryloylglycinamide), hydroxypropylcellulose, poly (vinylcaprolactame), and polyvinyl methyl ether.
  • the comonomer is a group consisting of allylamine (AA), dimethylaminoethyl methacrylate (DMAEMA), dimethylaminoethyl acrylate (DMAEA), acrylic acid (AAc), polyethylene glycol (PEG), and methacrylic acid (MAAc).
  • AA allylamine
  • DMAEMA dimethylaminoethyl methacrylate
  • DAEA dimethylaminoethyl acrylate
  • acrylic acid AAc
  • PEG polyethylene glycol
  • MAAc methacrylic acid
  • the hydrogel functional layer is, poly (N-isopropyl acrylamide-co-allylamine) [poly (N-isopropyl acrylamide-co-allylamine): poly (NIPAM-co-AA)], poly ( N-isopropyl acrylamide-co-2- (dimethylamino) ethyl methacrylate) [poly (N-isopropyl acrylamide-co-2- (dimethylamino) ethyl methacrylate): poly (NIPAM-co-DMAEMA)], poly (N-isopropyl acrylamide-co-2- (dimethylamino) ethyl acrylate) [poly (N-isopropyl acrylamide-co-2- (dimethylamino) ethyl acrylate), poly (NIPAM-co-DMAEA)], poly (N-isopropyl acrylamide-co-acrylic acid) [poly (N-isopropyl
  • the main monomer may be N-isopropyl acrylamide (temperature sensitive hydrogel), and the comonomer may be acrylic acid (pH sensitive hydrogel).
  • the crosslinking agent may be MBA (N, N'-methylene-bis-acrylamide).
  • the hydrogel functional layer may further include an initiator (initiator) as an element for starting the polymerization reaction, the ammonium persulfate (APS) may be used as the initiator.
  • an initiator initiator
  • APS ammonium persulfate
  • the part where the physical properties of the hydrogel functional layer is changed may be a part of the entire hydrogel functional layer or the surface of the hydrogel functional layer.
  • the activation layer may be equal to the thickness of the entire hydrogel functional layer or smaller than the thickness of the hydrogel functional layer.
  • the activation layer may be formed over a certain depth from the surface of the hydrogel functional layer.
  • the binding media formed on the hydrogel functional layer may include carbodiimide crosslinks, Schiff base crosslinks, Azlactone crosslinks, carbonyl diimidazole (CDI) crosslinks, Iodoacetyl crosslinks, Hydrazide crosslinks, Mannich crosslinks, Alternatively, it may be formed on the hydrogel functional layer by maleimide crosslinking.
  • a binding mediator can be formed on the surface of the hydrogel functional layer by using the carboxylic acid functional group (COOH) present on the hydrogel functional layer surface and the NH 2+ group present in the protein.
  • EDC (1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride
  • DCC dicyclohexyl carbodiimide
  • NaCNBH3 sodium cyanoborohydride
  • Azlactone CDI (Carbonyl diimidazole) ), Iodoacetyl, Hydrazide, diaminodipropylamine (DADPA), and at least one crosslinking agent selected from the group consisting of NHS esters (N-hydroxysuccinimide esters)
  • the crosslinking agent can be adjusted to control the bonding properties between the binding media substrate and the hydrogel functional layer.
  • the hydrogel functional layer may further include nanoparticles. By further injecting nanoparticles into the hydrogel functional layer, the following advantages arise.
  • protein multiple binding can be detected using simple image processing and analysis techniques such as mobile phone camera photography.
  • various mobile devices can be equipped with the ability to measure protein multiple bonds. Briefly, using a handheld device with a light emitting unit such as a camera and an LED, the optical property changes occurring on a biochip including a hydrogel functional layer implanted with nanoparticles are captured, and the captured images are processed to process protein multiplexing. Can be detected. In this way it can be utilized for personal portable diagnostic devices, point of care testing (POCT), or remote diagnostics.
  • a light emitting unit such as a camera and an LED
  • the optical property changes occurring on a biochip including a hydrogel functional layer implanted with nanoparticles are captured, and the captured images are processed to process protein multiplexing. Can be detected. In this way it can be utilized for personal portable diagnostic devices, point of care testing (POCT), or remote diagnostics.
  • POCT point of care testing
  • ⁇ Type 1> hydrogel functional layer infused with nano particles modified with substrate + binding medium substrate
  • FIG 3 shows an embodiment in which nanoparticles are injected into a hydrogel functional layer.
  • a hydrogel functional layer is formed on the substrate (step (a)).
  • Nanoparticles having a bonding medium substrate attached to the hydrogel functional layer are injected (step (b)).
  • the nanoparticles are rearranged in the hydrogel functional layer through dimerization or multiple bonds between the binding media substrate attached to the nanoparticles and the target protein (step (c)). At this time, the diswelling of the hydrogel functional layer also occurs.
  • the rearrangement of the nanoparticles and the dewelling of the hydrogel functional layer reduce the distance between the nanoparticles, thereby changing physical properties such as light transmittance of the hydrogel functional layer.
  • the hydrogel functional layer can be recycled.
  • the binding media substrate and nanoparticles used can be separated by lowering the pH of the hydrogel functional layer.
  • ⁇ Type 2> hydrogel functional layer implanted with nano particles by modifying substrate + binding medium substrate
  • FIG. 4 illustrates another embodiment in which nanoparticles are injected into a hydrogel functional layer.
  • a hydrogel functional layer modified with a bonding medium substrate is formed on the substrate (step (a)). Nanoparticles are injected into the hydrogel functional layer (step (b)). Dewelling occurs in the hydrogel functional layer while the binding mediator of the hydrogel functional layer is dimerized or multiplexed with the target protein (step (c)).
  • the diswelling of the hydrogel functional layer reduces the distance between the nanoparticles injected into the hydrogel functional layer and changes the physical properties such as light transmittance of the hydrogel functional layer.
  • the physical property of the hydrogel functional layer may be a refractive index.
  • the transducer 120 converts a change in physical properties of the hydrogel functional layer caused by multiple bonds between the target protein and the binding media substrate administered to the biochip 100 to a displacement signal. It will be apparent to those skilled in the art that the function and physical structure of the transducer 120 may be appropriately modified according to the application of the biochip 100 described herein.
  • the transducer 120 changes the refractive index of the hydrogel functional layer due to multiple bonds between the target protein and the binding mediator. Outputs a displacement signal corresponding to.
  • the transducer 120 for transmitting the displacement signal to the external analysis device according to various embodiments will be described in detail.
  • the transducer 120 may be an electrochemical sensor.
  • the electrochemical sensor technology captures biophenomena including multiple protein bonds occurring in at least a portion of the sensing region 110 and changes in physical properties of the hydrogel functional layer and converts them into electrical information.
  • the electrical information may be any one of voltage, current, and impedance.
  • CMOS complementary metal oxide semiconductor
  • CCD charge coupled device
  • the dewelling occurs in at least a portion of the hydrogel functional layer, and the dewelling causes the at least part of the hydrogel functional layer to
  • the physical properties of some areas change.
  • This physical characteristic may be an electrical characteristic, and the electrical characteristic may be any one of voltage, current, and impedance.
  • the electrical properties of the hydrogel functional layer change, the electrical information output from the electrochemical sensor is changed, and the amount of change of the electrical information is transferred to external analysis equipment through separate data-out circuitry to calculate the protein multiple binding amount. can do.
  • Electrochemical sensors can be fabricated in 2-D arrays. Each electrochemical sensor can have independent data-out output terminals, and each output terminal delivers output displacement information for that electrochemical sensor to external analysis equipment.
  • the transducer 120 may be an optical fiber.
  • 5 illustrates an embodiment of a biochip using an optical fiber.
  • the hydrogel functional layer 211 is positioned between two separate optical fibers 120, and an optical signal output through a laser light source is passed through one optical fiber.
  • an optical signal output through a laser light source is passed through one optical fiber.
  • the hydrogel functional layer Due to dewelling occurring in at least a portion of the region 211, light transmission characteristics of at least a portion of the hydrogel functional layer 211 are changed.
  • the light transmission characteristic may be any one of a change in optical power and a change in focal length of the optical signal.
  • the sensing region 110 may be designed in the shape of a concave or convex lens.
  • FIG. 6 shows another example of a biochip using an optical fiber.
  • the sensing region 110 is positioned on an optical fiber having a thin radius.
  • the hydrogel functional layer 211 may be Diswelling occurs in at least some regions, and the diswelling changes the refractive index characteristics of at least some regions of the hydrogel functional layer 211.
  • the optical signal is applied to one end of the tapered optical fiber and the output signal is measured at the other end, the output signal displacement occurs according to the degree of protein multiplexing.
  • the displacement of the output signal may be a shift in the resonant wavelength, a change in the output optical signal magnitude.
  • the hydrogel functional layer 211 may be formed in a sphere shape in order to increase the variation of the output displacement signal.
  • the spherical hydrogel functional layer 211 may be formed by applying a hydrogel to the outside of a sphere made of silica. As described above, when multiple bonds occur between the binding medium substrate formed on the hydrogel functional layer 211 and the target protein, dewelling occurs in at least a portion of the hydrogel functional layer 211, and due to the dewelling The refractive index characteristic of at least a portion of the hydrogel functional layer 211 is changed.
  • the optical signal is applied at one end of the tapered optical fiber and the output signal is measured at the other end, it is transmitted through the spherical hydrogel functional layer 211 and the tapered optical fiber.
  • the displacement signal is output due to the change in the combined phenomenon of the optical signals.
  • the displacement signal may be a signal corresponding to the shift of the resonance frequency or the change of the output signal magnitude. This displacement signal can be analyzed to determine the amount of multiple binding of the target protein.
  • the change in the physical properties of the hydrogel functional layer becomes larger.
  • multiple bonds between the target protein and the binding mediator can be achieved using a lookup table (not shown) generated by comparing the amount of multiple bonds with changes in physical properties of the hydrogel functional layer 211. Detect and measure the amount of multiple bonds.
  • the lookup table may be recorded in a memory connected to an external analysis device such as a biosensor connected to a biochip through a physical interface.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention concerne une biopuce permettant de détecter et d'analyser de manière détectable des liaisons multiples et des liaisons simples d'une protéine. Une biopuce selon un mode de réalisation comprend une zone de détection dans laquelle une couche fonctionnelle d'hydrogel présentant un médiateur de liaison formé sur sa surface et présentant un changement de propriété physique dû à la réaction d'une protéine cible et du médiateur de liaison est formée ; et un transducteur émettant en sortie un signal de déplacement correspondant au changement de propriété physique, la couche fonctionnelle d'hydrogel comprenant en outre des nanoparticules.
PCT/KR2018/003918 2017-04-04 2018-04-03 Biopuce Ceased WO2018186657A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR10-2017-0043756 2017-04-04
KR20170043756 2017-04-04
KR10-2018-0007781 2018-01-22
KR20180007781 2018-01-22
KR10-2018-0038036 2018-04-02
KR1020180038036A KR102037800B1 (ko) 2017-04-04 2018-04-02 바이오 칩

Publications (1)

Publication Number Publication Date
WO2018186657A1 true WO2018186657A1 (fr) 2018-10-11

Family

ID=63712311

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/003918 Ceased WO2018186657A1 (fr) 2017-04-04 2018-04-03 Biopuce

Country Status (1)

Country Link
WO (1) WO2018186657A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030031895A (ko) * 2000-04-22 2003-04-23 엠-바이오테크, 인코포레이티드 하이드로겔 바이오센서 및 이를 이용한 건강경보시스템
KR20070085460A (ko) * 2004-10-28 2007-08-27 바이엘 헬스케어 엘엘씨 하이드로겔 조성물
US20080029390A1 (en) * 2006-02-27 2008-02-07 Joelle Roche Hydrogel for an intravenous amperometric biosensor
US20130245402A1 (en) * 2012-03-13 2013-09-19 Babak Ziaie Sensor having ferrogel with magnetic particles
US8840839B2 (en) * 2007-04-05 2014-09-23 Koninklijke Philips N.V. Hydrogel based device for detecting an environmental state

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030031895A (ko) * 2000-04-22 2003-04-23 엠-바이오테크, 인코포레이티드 하이드로겔 바이오센서 및 이를 이용한 건강경보시스템
KR20070085460A (ko) * 2004-10-28 2007-08-27 바이엘 헬스케어 엘엘씨 하이드로겔 조성물
US20080029390A1 (en) * 2006-02-27 2008-02-07 Joelle Roche Hydrogel for an intravenous amperometric biosensor
US8840839B2 (en) * 2007-04-05 2014-09-23 Koninklijke Philips N.V. Hydrogel based device for detecting an environmental state
US20130245402A1 (en) * 2012-03-13 2013-09-19 Babak Ziaie Sensor having ferrogel with magnetic particles

Similar Documents

Publication Publication Date Title
CN107402199B (zh) 基因测序芯片及其测序方法以及基因测序装置
US10359422B2 (en) Biochip and method for manufacturing biochip
TW200420728A (en) Optical DNA sensor, DNA reading apparatus, identification method of DNA and manufacturing method of optical DNA sensor
RU2673288C2 (ru) Детектируемые матрицы, системы для диагностики и способы их получения и применения
AU2015213655A1 (en) Nondestructive collection of evidence
JP2002031617A5 (fr)
WO2021177725A1 (fr) Dispositif électronique portable
US20090170124A1 (en) Hydrogel thin film for use as a biosensor
CN107638176B (zh) 一种水凝胶在制备检测脑皮层电图的电极中的应用
WO2012081873A2 (fr) Testeur de fatigue à ultra-haute fréquence
US9632033B2 (en) Cellular phone based optical detection of specific nucleic acid sequences
WO2020101347A1 (fr) Dispositif de détection de biomolécules mettant en oeuvre des micropores
Abdullah et al. Ultrasimple single-cell detection of multiple cytokines by a nanowell chip integrated with encoded microarrays
KR102037800B1 (ko) 바이오 칩
WO2018186657A1 (fr) Biopuce
CN110082334A (zh) 一种多通道光纤荧光传感器
US20070131552A1 (en) Biomolecule detection device, mobile phone for biomolecule detection, and biomolecule detection method
WO2010140773A2 (fr) Biocapteur destiné à mesure un biomatériau
CA3053677A1 (fr) Produit, methode et systeme d'analyse d'imagerie par telephone intelligent pour la pour la detection d'ions de mercure
Xie et al. Photosensitive Hydrogel with Temperature‐Controlled Reversible Nano‐Apertures for Single‐Cell Protein Analysis
WO2011027979A2 (fr) Biodétecteur capable de reconnaître automatiquement des codes et procédé de reconnaissance de code l'utilisant
KR20220131188A (ko) 바이오센서 및 갭 전극을 이용하는 바이오센서에 적용되는 전극의 제조 방법
WO2017116078A1 (fr) Biopuce et procédé de fabrication de biopuce
JP5093633B2 (ja) 複数物質応答性ゲルおよびその製造方法並びにその利用
WO2012115349A2 (fr) Procédé de détection de biomolécules à l'aide d'un écran tactile capacitif

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18781664

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18781664

Country of ref document: EP

Kind code of ref document: A1