miRprimer designs primers for PCR amplification of microRNAs as described (Busk (2014). A tool for design of primers for microRNA-specific quantitative RT-qPCR. BMC Bioinformatics. 15, 29) for use with the method miR-specific RT-qPCR (Cirera, S., and Busk, P.K. (2014). Quantification of miRNAs by a simple and specific qPCR method. Methods in Molecular Biology. 1182, 73-81.).
The program was written in Ruby and is available as source code for developers and as an .exe file for easy use.
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Apache License V2.0Follow miRprimer
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I downloaded the file according to your instructions, but cannot run miprimar2. I can only run miprimar.