CN106070237A - A kind of Fluxapyroxad-containsterilization and the bactericidal composition of nitrile bacterium azoles and application thereof - Google Patents

Abstract

The invention discloses a bactericidal composition containing flufenapyroxam and myclobutanil, the active components of the fungicide composition are flufenapyroxam and myclobutanil, and the quality of flufenapyroxam and myclobutanil The ratio is 1:20 to 20:1, and the mass percentage of active components in the composition is 5-60%. The bactericide composition of the present invention has high bactericidal activity, delays the occurrence and development of drug resistance of pathogens at the same time, and reduces the dosage of preventive and control drugs in actual production, and is economical, efficient, safe and environmentally friendly. The invention has good control effects on various crop diseases, and the control effect on rice sheath blight is particularly remarkable.

Description

A kind of bactericidal composition containing flupyroxyroxad and myclobutanil and its application

technical field

The invention relates to the field of agricultural fungicides, in particular to a fungicidal composition containing flufenapyroxystrobin and myclobutanil and an application thereof.

Background technique

Rice sheath blight is a worldwide epidemic disease caused by Rhizoctonia solani, and it is also one of the most important diseases of rice in my country. Its distribution is very wide, and it occurs in all major rice producing areas in my country, especially in the middle and lower reaches of the Yangtze River and South China. The damage is very serious, resulting in a sharp decline in yield. Breeding and promoting disease-resistant varieties is one of the important measures to prevent and control rice sheath blight. In recent years, although scholars at home and abroad have made a lot of progress in disease-resistant breeding, there is still no one that is completely resistant to rice sheath blight. Immunized rice varieties. At present, the control of rice sheath blight still relies on chemical control. At present, my country has been using Jinggangmycin as the main agent in the control of rice sheath blight. In the early stage of the disease, that is, when the rate of diseased plants in the late tillering stage of rice reaches about 15%, the spraying control has achieved certain results. However, it has been reported that rice sheath blight in some areas of my country has developed resistance to Jinggangmycin, resulting in a continuous decline in the control effect of the drug. How to continue to control rice sheath blight, especially how to screen out alternative agents for the control of rice sheath blight, at the same time control Jinggangmycin resistance and promote sustainable control of rice sheath blight, has become a challenge faced by the majority of scientific and technological workers and rice growers. major challenge.

Fluxapyroxad, chemical name: 3-(difluoro)-1-methyl-N-(3',4',5'-trifluoro[1,1'-biphenyl]-2-yl )-1-H-pyrazole-4-amide, flupyroxyroxamide is a pyrazole amide fungicide, succinate dehydrogenase inhibitor, both prevention and control. It can inhibit fungal respiration, inhibit spore germination, germination tube elongation, hyphal growth and sporulation. Can be used to control and suppress a variety of fungal diseases on a variety of crops. Combines superior long-lasting potency with sustained delivery properties.

The molecular formula of flufenapyroxamide is C ₁₈ H ₁₂ F ₅ N ₃ O, and the structural formula is:

Myclobutanil (Myclobutanil), chemical name: 2-(4-chlorophenyl)-2-(1H,1,2,4-triazole-1-methyl)capronitrile, is a class of protective and therapeutic activity systemic triazole fungicides. It mainly inhibits the biosynthesis of ergosterol of pathogenic bacteria, and has a good control effect on Ascomycetes and Basidiomycetes. The agent has a long-lasting effect, is safe to crops, and has a certain growth-stimulating effect. Suitable crops and safety: apples, pears, stone fruits, grapes, gourds, horticultural ornamental crops, wheat, barley, oats, cotton and rice, etc. Safe for crops. It has good control effect on powdery mildew, scab, rot, rust, brown spot, gray spot, and smut.

Myclobutanil molecular formula: C ₁₅ H ₁₇ ClN ₄ , molecular structural formula:

Fluxapyroxad and myclobutanil are low-toxic or slightly toxic pesticides, which are high-efficiency, low-toxic, and environmentally friendly fungicides. Myclobutanil mainly acts on the synthesis of ergosterol in pathogenic bacteria, while flufenapyramide acts on the respiration of bacteria, so as to achieve the purpose of sterilization, and there is no cross-resistance between the two. However, many studies have shown that if these two agents are used alone to prevent and control diseases, they can cause pathogenic bacteria to develop drug resistance in a short period of time, resulting in reduced control efficiency, or even failure of control. The Fungicide Resistance Action Committee (FRAC) suggested that the combined use of agents with different mechanisms of action can avoid or delay the emergence of drug resistance. Therefore, the combination of the two into a fungicidal composition can not only effectively solve the problem of rice sheath blight control, but also avoid or delay the development of resistance of pathogens to it.

Contents of the invention

The invention provides a bactericidal composition containing flupyroxyroxad and myclobutanil, which has high bactericidal activity, delays the occurrence and development of drug resistance of pathogens, and reduces the total dosage per unit area, which is economical, efficient and environmentally friendly .

Technical scheme of the present invention is:

A fungicide composition containing flufenapyroxam and myclobutanil, characterized in that, the active components of the fungicide composition are flufenapyroxam and myclobutanil, the composition of flufenapyroxam and myclobutanil The mass ratio is 1:20 to 20:1, and the mass percentage of active components in the composition is 5-60%.

The mass ratio of the active components flufenapyroxam to myclobutanil is 1:10 to 10:1.

The mass ratio of flufenapyroxad to myclobutanil is 1:3-8.

The mass ratio of flufenapyroxad to myclobutanil is 1:4-6.

The dosage form of the fungicide composition is suspension concentrate, emulsifiable concentrate, water dispersible granule or wettable powder.

The fungicide composition has higher control effects on various crop diseases, especially the control effect on rice sheath blight, and is used for the control of rice sheath blight.

Significant effect and advantage of the present invention are:

The present invention combines two types of fungicides with different mechanisms of action, i.e., the triazole fungicide myclobutanil and the pyrazole amide fungicide flufenapyramide, and processes them into a preparation that is easy to use in production and has high biological activity. The total dosage per unit area is reduced, which greatly reduces the cost of application, and at the same time delays the emergence and development of pathogenic drug resistance to a greater extent, reduces the risk of pathogenic fungi developing resistance to a single fungicide, and prolongs the life of the fungicidal composition. The service life of each component in the product can achieve economical, efficient and environmentally friendly effects.

The present invention has a higher control effect on fungal diseases of crops, and there is an obvious synergistic effect between the two components; it can replace some high-priced pesticide varieties, reduce the dosage of pesticides, effectively reduce ecological damage and environmental pollution, and can improve the quality of crops. Yield and quality; both flufenapyroxad and myclobutanil in the present invention are of low toxicity, and are safe to humans, animals, beneficial organisms, and the environment; and can delay the resistance of pathogens to a single agent.

First measure the EC ₅₀ (drug safety index) of two single compounds (hereinafter referred to as the medicament) of flufenapyroxad and myclobutanil, and set the combination ratio of the two medicaments according to the Wadley method according to the EC ₅₀ of the two single medicaments, and according to the Wadley increase Efficiency coefficient C value, determine the appropriate composition ratio, and then verify it according to Sun Yunpei's method.

The test object is rice sheath blight fungus, but not limited thereto. The test adopts the mycelial growth assay method, and the drug-containing medium is made according to different treatments, and each treatment is repeated 4 times. After 3 days of inoculation, the growth inhibitory effect of each treatment on the rice sheath blight fungus was investigated. Through the linear regression analysis between the probability value of the control effect and the logarithmic value of the serial drug concentration, the EC50 of each drug was obtained, and the synergistic coefficient (SR) was calculated according to the Wadley method, and a comprehensive evaluation was made accordingly. SR≤0.5 means antagonistic effect, 0.5<SR<1.5 means additive effect, SR≥1.5 means synergistic effect. The calculation method of SR value is as follows:

EC ₅₀ (theoretical value)=(a+b)/(a/EC _50A +b/EC _50B )

SR＝EC ₅₀ (theoretical value)/EC ₅₀ (actual value)

SR ≤ 0.5, the two agents mixed with antagonistic effect

SR＝0.5～1.5, the two medicines are mixed and have additive effect

SR ≥ 1.5, the combination of the two agents has a synergistic effect

Table 1 Fluxapyroxad and myclobutanil composition to the Wadley method determination result of rice sheath blight

Note: F is fluxapyroxad, J is myclobutanil.

From the above data, it can be analyzed that different proportions of flufenapyroxad and myclobutanil compositions have different antibacterial effects on rice sheath blight bacteria. Generally speaking, the ratio is in the range of 1:20 to 20:1 All have synergistic or additive effects, and the maximum synergistic ratio is 5:1.

The present invention will be further described below in conjunction with the examples, but not limited thereto.

detailed description

Example 1 (preparation method of 60% flufenapyr-myclobutanil wettable powder)

Raw material formula: by mass percentage

Preparation method: uniformly mix the above-mentioned substances, and pulverize the mixture through a jet mill to obtain a wettable powder.

Embodiment 2 (preparation method of 30% flufenpyrazole myclobutanil suspension concentrate)

Preparation method: According to the formula, weigh each component in proportion, put it into a ball mill and mill it for 30 minutes, filter, pump it into the dispersion tank for 3 minutes, and then grind it fully with a sand mill to control the particle diameter of the solid component within 2 microns , and stir evenly after grinding to obtain a suspension product.

Embodiment 3 (preparation method of 40% flufenapyramide myclobutanazole water-dispersible granule)

Preparation method: Mix flufenapyroxad, myclobutanil, sodium methyl fatty acid amides benzene sulfonate, sodium carbonate, and attapulgite evenly, and then pulverize with a jet mill to a particle size of 5 μm to obtain the mother powder. Fully mixed with sodium lignosulfonate, granulated, dried, and sieved to obtain water-dispersible granules.

Example 4 (20% flufenapyr · myclobutanil suspension concentrate)

Preparation method: with embodiment 2.

Example 5 (45% flufenapyr myclobutanil emulsifiable concentrate)

Raw material formula: by mass percentage

Preparation method: fully dissolve the active component flufenapyroxad in a xylene reactor to make liquid A; fully dissolve the active component myclobutanil in a xylene reactor to make liquid B; keep stirring Mix liquid A into liquid B in the state, stir well, add anhydrous calcium salt and NP-10, and then adjust the content with solvent xylene.

Embodiment 6 (preparation method of 48% flufenapyramide myclobutanil emulsifiable concentrate)

Raw material formula: by mass percentage

Preparation method: with embodiment 5.

Control effect test:

The field control effect test of rice sheath blight was carried out in Fengyang County, the test area was 0.3hm ² , each treatment was repeated three times, the control was sprayed at the rice booting stage, and the control effect was investigated 15 days after spraying. The control results are shown in Table 2 . The results show that the fungicidal composition containing flufenapyroxad and myclobutanil has a good control effect on rice sheath blight, and can achieve a higher control effect at the same dosage or at a lower dosage, and the fungicidal composition The control bacteria of each treatment were greater than 85%, which was significantly higher than that of the control agent.

Table 2 Field efficacy of flufenapyroxad-myclobutanil fungicidal composition in controlling rice sheath blight*

It can be seen from the data in Table 2 that the fungicidal composition containing flufenapyroxad and myclobutanil has good control effects on rice sheath blight, and the control effects are different with different proportions. It is worth noting that when the ratio of flufenapyroxad and myclobutanil is 5:1, compared with other treatments, it has a higher control effect on diseases at a lower dosage, showing better Synergistic effect; and indoor biotest results also showed that when the ratio of flufenapyroxad and myclobutanil was 5:1, the measured synergistic coefficient was the highest, and the results of the two were consistent.

Finally, it should be noted that the above examples are only some specific embodiments of the present invention. Apparently, the present invention is not limited to the above embodiments, and many modifications are possible. All deformations that can be directly derived or associated by those skilled in the art from the content disclosed in the present invention are considered to be within the protection scope of the present invention.

Claims (6)

1. a Fluxapyroxad-containsterilization and the bactericidal composition of nitrile bacterium azoles, it is characterised in that active group of described bactericidal composition Being divided into fluxapyroxad and nitrile bacterium azoles, fluxapyroxad is 1:20 to 20:1 with the mass ratio of nitrile bacterium azoles, activity group in compositions Dividing weight/mass percentage composition is 5-60%.

Fluxapyroxad-containsterilization the most according to claim 1 and the bactericidal composition of nitrile bacterium azoles, it is characterised in that: described work Property component fluxapyroxad and nitrile bacterium azoles mass ratio be 1:10 to 10:1.

Fluxapyroxad-containsterilization the most according to claim 1 and the bactericidal composition of nitrile bacterium azoles, it is characterised in that: described fluorine Azoles bacterium amide is 1:3-8 with the mass ratio of nitrile bacterium azoles.

Fluxapyroxad-containsterilization the most according to claim 1 and the bactericidal composition of nitrile bacterium azoles, it is characterised in that: described fluorine Azoles bacterium amide is 1:4-6 with the mass ratio of nitrile bacterium azoles.

Fluxapyroxad-containsterilization the most according to claim 1 and 2 and the bactericidal composition of nitrile bacterium azoles, it is characterised in that: described The dosage form of bactericidal composition is suspending agent, cream, water dispersible granules or wettable powder.

6., according to the Fluxapyroxad-containsterilization described in any one of claim 1-2 and the application of the bactericidal composition of nitrile bacterium azoles, it is special Levy and be: for the preventing and treating of rice sheath blight disease.