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HUP9901640A2 - Foszfolipáz aktivítású fehérje - Google Patents

Foszfolipáz aktivítású fehérje

Info

Publication number
HUP9901640A2
HUP9901640A2 HU9901640A HUP9901640A HUP9901640A2 HU P9901640 A2 HUP9901640 A2 HU P9901640A2 HU 9901640 A HU9901640 A HU 9901640A HU P9901640 A HUP9901640 A HU P9901640A HU P9901640 A2 HUP9901640 A2 HU P9901640A2
Authority
HU
Hungary
Prior art keywords
protein
cleaved
phospholipase activity
phospholipase
fermentation
Prior art date
Application number
HU9901640A
Other languages
English (en)
Inventor
Gerald Jungschaffer
Quoc Khanh
Fridolin Löffler
Erwin Schuster
Bruno Sprössler
Sabine Wolf
Original Assignee
Röhm GmbH.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Röhm GmbH. filed Critical Röhm GmbH.
Publication of HUP9901640A2 publication Critical patent/HUP9901640A2/hu
Publication of HUP9901640A3 publication Critical patent/HUP9901640A3/hu
Publication of HU224831B1 publication Critical patent/HU224831B1/hu

Links

Classifications

    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
    • A21D2/24—Organic nitrogen compounds
    • A21D2/26—Proteins
    • A21D2/267—Microbial proteins
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y10S435/917—Aspergillus niger

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A találmány egy főszfőlipáz aktivitású fehérjére vőnatkőzik, amelyrejellemző, hőgy az Aspergillűs-lizőfőszfőlipáz érett szekvenciájával,vagy egy abból levezethető szekvenciával rendelkezik, és legalább egyhelyen el lehet hasítva, mimellett ha el van hasítva, akkőr a hasítőttdarabők legalább egy, redűkáló körülmények között hasítható kötésselkapcsőlódnak, vagy a nem kapcsőlt hasítőtt darabők közül legalább egyfőszfőlipáz aktivitású. A találmány tárgyát képezi ennek a fehérjénekaz előállítása is egy alkalmas módőn transzfőrmált lizőfőszfőlipáz-termelő gazdaszervezet megfelelő tenyésztőközegben végzettfermentálásával és a főszfőlipáz aktivitású fehérjének a sejtmenteskűltúraszűrletből történő izőlálásával, ahől a fermentálást a savastóla gyengén bázikűsig terjedő pH-tartőmányban végzik. ŕ
HU9901640A 1997-01-16 1998-01-08 Protein with phospholipase activity HU224831B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19701348A DE19701348A1 (de) 1997-01-16 1997-01-16 Protein mit Phospholipaseaktivität
PCT/EP1998/000081 WO1998031790A1 (de) 1997-01-16 1998-01-08 Protein mit phospholipaseaktivität

Publications (3)

Publication Number Publication Date
HUP9901640A2 true HUP9901640A2 (hu) 1999-08-30
HUP9901640A3 HUP9901640A3 (en) 2001-09-28
HU224831B1 HU224831B1 (en) 2006-02-28

Family

ID=7817548

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9901640A HU224831B1 (en) 1997-01-16 1998-01-08 Protein with phospholipase activity

Country Status (16)

Country Link
US (1) US6140094A (hu)
EP (1) EP0904357B1 (hu)
CN (1) CN1152954C (hu)
AR (1) AR010099A1 (hu)
AT (1) ATE348151T1 (hu)
AU (1) AU6208098A (hu)
BR (1) BR9805893A (hu)
CA (1) CA2243476C (hu)
DE (2) DE19701348A1 (hu)
DK (1) DK0904357T3 (hu)
ES (1) ES2275299T3 (hu)
HU (1) HU224831B1 (hu)
MY (1) MY117580A (hu)
PT (1) PT904357E (hu)
TW (1) TW565610B (hu)
WO (1) WO1998031790A1 (hu)

Families Citing this family (42)

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DE69604491T3 (de) 1995-06-07 2008-09-25 Danisco A/S Methode zur verbesserung der eigenschaften von mehlteig, sowie zusammensetzung zur teigverbesserung und verbesserte nahrungsmittel
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
DE19620649A1 (de) * 1996-05-22 1997-11-27 Roehm Gmbh Rekombinant hergestellte Lysophospholipase aus Aspergillus
EP1433852B1 (en) 1997-04-09 2006-06-28 Danisco A/S Use of lipase for improving doughs and baked products
ES2188143T5 (es) † 1998-04-20 2008-11-01 Novozymes A/S Preparacion de masa y de productos horneados.
EP1098988B9 (en) 1998-07-21 2007-10-24 Danisco A/S Foodstuff
AU2011204998B2 (en) * 1998-11-27 2013-02-14 Novozymes A/S Lipolytic enzyme variants
CA2353379C (en) 1998-11-27 2011-01-04 Novozymes A/S Lipolytic enzyme variants
US7312062B2 (en) * 1998-11-27 2007-12-25 Novozymes A/S Lipolytic enzyme variants
ATE494366T1 (de) 1999-10-14 2011-01-15 Novozymes As Lysophospholipase aus aspergillus
US20020192792A1 (en) 2000-04-28 2002-12-19 Palle Schneider Laccase mutants
EP1352057B1 (en) 2001-01-10 2009-03-25 Novozymes A/S Lipolytic enzyme variant
CN1526013A (zh) * 2001-02-23 2004-09-01 诺维信公司 脂解酶基因
ATE362315T1 (de) 2001-05-18 2007-06-15 Danisco Verfahren zur herstellung von teig mit einem enzym
AU2003203139B2 (en) 2002-01-16 2007-11-08 Novozymes A/S Lipolytic enzyme variants and method for their production
CN100545261C (zh) * 2002-05-21 2009-09-30 Dsmip资产有限公司 新的磷脂酶及其用途
US6773731B2 (en) * 2002-10-18 2004-08-10 James S. Campbell Liquid egg yolk product comprising lysophospholipoprotein
US20050196766A1 (en) * 2003-12-24 2005-09-08 Soe Jorn B. Proteins
MXPA05007653A (es) 2003-01-17 2005-09-30 Danisco Metodo.
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
CN1328375C (zh) * 2003-03-04 2007-07-25 独立行政法人制品评价技术基盘机构 来自于曲霉菌的磷脂酶a2
CN101319204A (zh) * 2003-04-28 2008-12-10 诺维信公司 磷脂酶和其生产方法
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
EP2267107A3 (en) 2004-07-16 2011-05-25 Danisco A/S Lipolytic Enzyme. Uses Thereof In The Food Industry
US20070148311A1 (en) * 2005-12-22 2007-06-28 Bunge Oils, Inc. Phytosterol esterification product and method of make same
DE102006046857A1 (de) 2006-10-02 2008-04-03 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Lysophospholipasen
DE102006046719A1 (de) 2006-10-02 2008-04-03 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Phospholipasen
AU2007344910B2 (en) 2007-01-25 2013-03-28 Dupont Nutrition Biosciences Aps Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
CN101589142B (zh) * 2007-01-30 2012-06-06 三菱化学食品株式会社 甘油糖脂脂肪酶
US8460905B2 (en) * 2007-09-11 2013-06-11 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time
US8956853B2 (en) * 2007-01-30 2015-02-17 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases
US8241876B2 (en) 2008-01-07 2012-08-14 Bunge Oils, Inc. Generation of triacylglycerols from gums
EP2249156A1 (en) * 2009-04-29 2010-11-10 Aterovax Enzymatic assay for the quantitative determination of phospholipase A1 or A2 activity in a sample based on the use of a solid phase coated with a fluorochrome-labelled substrate
DE102009051013A1 (de) 2009-10-28 2011-06-09 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Phospholipasen
CA2788148C (en) 2010-03-12 2018-03-13 Dupont Nutrition Biosciences Aps An enzymatic process for refining a plant oil
AR085251A1 (es) 2011-02-23 2013-09-18 Danisco Proceso para tratar aceite vegetal
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
DK2840900T3 (da) 2012-04-25 2019-09-09 Novozymes As Fremgangsmåde til bagning
RU2763469C2 (ru) 2017-02-20 2021-12-29 Новозимс А/С Липолитический фермент для применения в хлебопечении

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2180541T5 (es) * 1992-06-16 2008-12-01 Sankyo Lifetech Company Limited Nueva fosfolipasa a1, procedimiento para su preparacion y uso de la misma.
BR9506861A (pt) * 1994-02-22 1997-09-23 Novo Nordisk As Processo para preparar e produzir uma variante de uma enzima lipolítica originária variante de enzima liplítica construção de dna vetor célula hospedeira aditivo detergente e composição detergente
DE19527274A1 (de) * 1995-07-26 1997-01-30 Metallgesellschaft Ag Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase
DE19620649A1 (de) * 1996-05-22 1997-11-27 Roehm Gmbh Rekombinant hergestellte Lysophospholipase aus Aspergillus

Also Published As

Publication number Publication date
ATE348151T1 (de) 2007-01-15
CA2243476C (en) 2009-01-06
CN1152954C (zh) 2004-06-09
TW565610B (en) 2003-12-11
PT904357E (pt) 2007-03-30
EP0904357A1 (de) 1999-03-31
DE59813840D1 (de) 2007-01-25
CA2243476A1 (en) 1998-07-23
HU224831B1 (en) 2006-02-28
AR010099A1 (es) 2000-05-17
ES2275299T3 (es) 2007-06-01
US6140094A (en) 2000-10-31
HK1019074A1 (en) 2000-01-21
MY117580A (en) 2004-07-31
BR9805893A (pt) 1999-08-24
DK0904357T3 (da) 2007-04-10
AU6208098A (en) 1998-08-07
DE19701348A1 (de) 1998-07-23
WO1998031790A1 (de) 1998-07-23
CN1216061A (zh) 1999-05-05
HUP9901640A3 (en) 2001-09-28
EP0904357B1 (de) 2006-12-13

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Legal Events

Date Code Title Description
MM4A Lapse of definitive patent protection due to non-payment of fees