JPH05255045A - Hair grower - Google Patents

Abstract

PURPOSE:To provide a hair grower excellent in such effects as hair growing, anti-depilation, or dandruff prevention. CONSTITUTION:The objective hair grower comprises (A) at least one kind selected from the group consisting of low-molecular weight acidic mucopolysaccharides and their salts and (B) and extract from Aconiti tuber.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a hair nourishing agent which is excellent in hair growth effect, hair nourishing effect, hair loss prevention, dandruff prevention and the like.

[0002]

2. Description of the Related Art Alopecia is considered to be caused by various factors intricately intertwined with each other, and the mechanism of occurrence is still unclear. Conventionally, a hair nourishing agent containing various agents has been used for the prevention and treatment of hair loss, but a drug effective enough to suppress hair loss and promote hair growth and hair nourishment has not yet been found.

[0003] The fusi, which is used as a raw material for the hair nourishing agent of the present invention, has been described in China since ancient times as a herbal sutra and is known as an important crude drug that is widely used in various Kampo prescriptions in Kampo medicine. ) Aconitum species (Aconitum specie)
s) tuberous root. Japanese Patent Laid-Open No. 3-271213 proposes a hair growth and hair-growing agent characterized by containing an extract of the root, stem or leaf of aconite, which is the source plant, but the extract of aconite alone is used. The hair growth and hair growth effects were insufficient.

[0004]

DISCLOSURE OF THE INVENTION In view of the above circumstances, the present inventor has conducted extensive studies and as a result has found that at least one selected from the group of low molecular weight acidic mucopolysaccharides and salts thereof and an extract of aconidium. By blending, the safety is high,
The present inventors have completed the present invention by finding that they show excellent effects in promoting mouse hair growth and human hair growth, and exhibit a remarkable effect in preventing dandruff. That is, an object of the present invention is to provide a hair nourishing agent having excellent effects such as hair growth, hair nourishment, hair loss prevention, and dandruff prevention.

[0005]

The present invention is a hair nourishing agent containing at least one selected from the group of low-molecular-weight acidic mucopolysaccharides and salts thereof, and an extract of fusi.

As the acidic mucopolysaccharide used in the hair nourishing agent of the present invention, hyaluronic acid, dermatan sulfate, chondroitin 4-sulfate, chondroitin 6-sulfate, heparin, heparan sulfate and the like can be used, and all of them are known substances. Therefore, it is widely distributed in the animal body as a matrix component in cartilage, joints, eyeballs, skin and other connective tissues and bound to proteins.

The low-molecular-weight acidic mucopolysaccharide used in the present invention is a product obtained by further reducing the molecular weight of natural products. The molecular weight of hyaluronic acid is 1,000 to less than 100,000, dermatan sulfate, chondroitin 4 Sulfuric acid and chondroitin 6-sulfate are preferably 1,000 to less than 10,000, and heparin and heparan sulfate are preferably 1,000 to less than 7,000.

The salt of the acidic mucopolysaccharide used in the present invention includes alkali metal salts such as sodium and potassium salts of the mucopolysaccharide, alkaline earth metal salts such as magnesium salt and calcium salt, ammonium salt and triethanol. Alkanolamine salts such as amine salts, basic amino acid salts such as lysine salts, arginine salts and histidine salts are preferred. Further, these acidic mucopolysaccharides can also be used as a free acid.

The acidic mucopolysaccharides and salts thereof used in the present invention can be obtained as follows. (1) Low molecular weight hyaluronic acid and its salt Commercially available hyaluronic acid having an average molecular weight of 1,000,000 5
g is dispersed and dissolved in 100 ml of 0.02 N hydrochloric acid aqueous solution, adjusted to pH 2-3, and heated to 65 ° C. in a warm bath while stirring. By changing the heating treatment time from 10 minutes to 120 minutes, the average molecular weight was changed from 1,000 to 10 minutes.
It is possible to obtain 10,000 hyaluronic acid. Then, after neutralizing with 0.1N sodium hydroxide, the solution was cooled to room temperature, and 3 times volume of ethanol was added to obtain a precipitate, which was then dried to obtain low-molecular weight hyaluronic acid sodium salt powder. In addition, this low molecular weight hyaluronic acid sodium salt is treated with an acid to give free hyaluronic acid, and then treated with another basic substance, for example, salts such as potassium salt, triethanol salt, L-arginine salt, etc. Prepared.

(2) Low molecular weight chondroitin tetrasulfate and its salt, etc. 5 g of commercially available sodium chondroitin tetrasulfate having an average molecular weight of 30,000 was dissolved in 50 ml of water and applied to an Amberlite IR-120 (H type) resin column. The effluent obtained through is warmed to 75 ° C. in a warm bath with stirring.
By changing the heating treatment time from 10 minutes to 120 minutes, chondroitin tetrasulfate having an average molecular weight of 1,000 to 10,000 can be obtained. Then
After neutralizing with 0.1 N sodium hydroxide, the solution was cooled to room temperature, and a precipitate obtained by adding 3 volumes of ethanol was centrifuged, washed with ethanol, and then dried to give low molecular weight chondroitin tetrasulfate. A powder of sodium salt was obtained.
Further, this low molecular weight chondroitin tetrasulfate sodium salt was treated with an acid to give a free acid, and then treated with another basic substance to prepare salts such as potassium salt, triethanol salt and L-arginine salt.

Further, low-molecular-weight chondroitin 6-sulfate, low-molecular-weight dermatan sulfate, low-molecular-weight heparin, low-molecular-weight heparan sulfate and the like were prepared in the free form or their respective salts in the same manner as in the above example of chondroitin 4-sulfate. In addition, the measuring method of the average molecular weight of the acidic mucopolysaccharide obtained by each treatment time is as follows.
Ogyi-Nelson) method was used.

[0012] On the other hand, the auxiliary of the raw herbal medicine used for the hair nourishing agent of the present invention is Ranunculaceae.
Aconitum plant (Aconitum specie)
As long as it is prepared from the tuberous root of s), its source and place of origin are not limited. Further, in general, aconite plants contain a highly toxic aconitine alkaloids, and since careful attention is required when using the aconitine alkaloids, more preferably, it is referred to as a processed aconitant prepared by detoxification such as heat treatment. It is better to use the one that is used.

[0013] Examples of the source plant of Fuzi are, for example, the cultivar Aconitum c.
armichaeli Debx), a wild species (Aconitum kusnezoffii Rei) from all over China.
chb. ), Etc. from Korea.
itum triphyllum Nakai), Aconitum coreanum
Rap. ), Japanese octopus beetle (Aconitu)
m japonicum Thunb. ), Aconitum chinese Pax
t. ) And the like. Particularly, the agar beetle produced in Sichuan Province of China is preferable in terms of quality stability and supply with a certain cultivation method.

As the detoxification processing method, Japanese Patent Publication No. 3
No. 7-18498, the tuber root is 100-130 degreeC in an autoclave, and the pressure is 1.0-1.5 kg /.
The method of preparing by applying pressure and heat treatment for 20 to 40 minutes per cm ² is a particularly preferable method because a crude drug can be stably produced in a simple, effective and safe manner. An example of the product prepared by this method is "Processed bush powder", which is a brand name product of the Japanese Pharmacopoeia. Also, as a method mainly used in China,
Soak the water-washed tuberous root in bitter juice for several days to dry it, or repeat the treatment such as steaming and boiling and then dry it, or wash the tuber with water and split it vertically and bury it in ash when fresh. Then, heating, pressurizing, etc. are applied and dried to prepare. As an example prepared by this method, a commercially available product of Fuzi gourd produced in Sichuan Province of China (referred to as Fuzi Fuzi in Japan) is preferable because it is sufficiently detoxified with a certain quality.

The extract of the adzuki bean of the present invention refers to a component which is transferred from the adsorbent to the solvent when the adsorbent is extracted with a solvent, and the extract is used in a dry state after removing the solvent. It can also be used in the state of being dissolved in a solvent. In the latter case, the extraction solvent will form part of the base in the hair restorer of the invention.

Solvents include water, alcohols such as methanol, ethanol, butanol ethers such as diethyl ether, dioxane, ketones such as acetone aliphatic hydrocarbons such as pentane, hexane, cyclohexane, esters such as ethyl acetate. Etc. can be used. When the extract is removed by solvent and used as a dried product, any of the above-mentioned solvents can be used alone or in combination. However, when the extract is used in a state of being dissolved in a solvent, it is preferable to use water, ethanol, or a mixture thereof, which has no harmful effect on the human body.

In the extraction, the adjunct may be used as it is, or may be crushed or crushed to improve the contact with the solvent. Although the ratio of the adjunct to the solvent is not particularly limited, the adjunct 1 is taken into consideration from the viewpoint of extraction efficiency and convenience.
Preference is given to using 100 to 5000 ml of solvent per 00 g. The extraction temperature is conveniently in the range of room temperature to the boiling point of the solvent under normal pressure, and the extraction time is preferably in the range of 30 minutes to 2 weeks, although it varies depending on the extraction temperature, the presence or absence of stirring and the like.

The present inventor has conducted extensive studies on the above-mentioned constituents to be incorporated in the hair nourishment, and as a result, the hair nourishing agent containing the extract of Fushi of the present invention and the low-molecular-weight acidic mucopolysaccharide and salts thereof has It was found that the synergistic effects have excellent hair growth effects (mouse hair growth promoting effect, human hair growth promoting effect), hair nourishing effect, hair loss prevention, dandruff prevention effect, and the like.

The blending ratio of the extract of Fuzi of the present invention and the low-molecular-weight acidic mucopolysaccharide and its salts is appropriately selected depending on the dosage form of the hair restorer, etc., but based on the total amount of the hair restorer. , The extract of Fuzi is 0.0001-20.
0 wt% (dry matter conversion), low molecular weight acidic mucopolysaccharides and salts thereof may be 0.01 to 3.0 wt%, and if the amount is less than the lower limit of each, sufficient effect for the purpose of the present invention is obtained. On the other hand, even if the upper limit is exceeded, improvement in the effect commensurate with the increase cannot be expected.

The hair nourishing agent of the present invention can be made into a dosage form such as a hair tonic, a hair lotion, a hair cream, a shampoo, a rinse, a hair foam and a hair gel according to a conventional method.

The hair nourishing agent of the present invention includes pigments, fragrances, germicides, preservatives, keratolytic agents, anti-inflammatory agents, anti-androgens,
Hair nourishing agents, vitamins, antioxidants, cooling agents, extracts of other herbal medicines and the like can be appropriately added within the range that achieves the object of the present invention.

[0022]

EXAMPLES The present invention will be described in detail below based on examples and comparative examples. The mouse hair growth promoting effect test method, human hair growth promoting effect test method and practical test method described in Examples are shown below.

(1) Mouse Hair Growth Promoting Effect Test C3H mice (male, 7 weeks old) were shaved with a hair clipper and a shaver on the central back hair of each mouse, and the sample was cut at the shaved site once a day per animal. 0.2 ml was applied. For each sample, 10 animals were used per group. On the fifteenth day after the start of the experiment, the back of the mouse was photographed, the hair cutting area and the hair growth area were calculated, the hair growth rate (%) was calculated, and the hair growth promoting effect was determined by the average value of 10 cases.

(2) Human hair growth promoting effect test method 10 hair test subjects who are male pattern baldness have circular hair with a diameter of 1 cm at 5 cm above the ears of the head. About 3 ml of the sample of Example or Comparative Example was applied to the left side of the shaved test site twice daily in the morning and evening, and compared with the untreated right side. Evaluation of the effect is 28 days after the start of the test,
20 hairs on each of the left and right test sites are shaved, and the average value (B) of the lengths of 20 hairs on the left side (applied Example or Comparative Example) is calculated as the length of 20 hairs on the right side (untreated). The value obtained by dividing the average value (A) of the sacks was obtained and evaluated. The determination result was shown by the average value of (B) / (A) of 10 test subjects.

(3) Practical test method 20 heads of androgenetic alopecia patients were applied with the sample twice daily in the morning and evening and continuously for 6 months to evaluate the effect. The test results were "hair bristling or increased hair loss", "less hair loss", "less dandruff" for each item of hair growth effect, hair loss prevention effect, and dandruff prevention effect. The number of people who answered

Production Example 1 (Preparation of extract of Fuzi) (1) A crushed product of dried root of Fuzi produced in Sichuan Province of China (commercially available as river crow or dried zizi) in an autoclave at 100 to 130 ° C. The pressure is adjusted to ¹ to 1.5 kg / cm ² , and pressure and heat treatment are performed for 40 minutes. Azushi 1 processed
00 g to 1500 ml of 70 v / v% ethanol (hereinafter, v / v% is abbreviated to%) at room temperature (20 to 25%).
C.) for 16 hours, the supernatant was collected by filtration, concentrated under reduced pressure at 45.degree. C. for 3 hours, and the solvent was distilled off to obtain 11.7 g of a brown extract.

(2) Preparation Example 1 100 g of Zhifu Province from China which was processed in the same manner as in (1) was immersed in 1500 ml of 40% ethanol for 16 hours at room temperature (20 to 25 ° C.),
The supernatant was collected by filtration and concentrated under reduced pressure at 45 ° C. for 4 hours, and the solvent was distilled off to obtain 13.4 g of a brown extract.

(3) 50 g of Zhuzi (commercially available as Zhizi) produced in Sichuan Province of China was immersed in 500 ml of 70% ethanol for 16 hours at room temperature (20 to 25 ° C.), and the supernatant was filtered. After collecting and concentrating at 45 ° C. under reduced pressure for 2 hours and distilling off the solvent, 5.3 g of a brown extract was obtained.

(4) Japanese non-Pharmacopoeia standard product processed bush powder 50
g to 500 ml of 40% ethanol at room temperature (20-2
After dipping at 5 ° C. for 10 hours, the supernatant was collected by filtration, concentrated under reduced pressure at 45 ° C. for 3 hours, and the solvent was distilled off to obtain 6.1 g of a brown extract.

Examples 1 to 10 and Comparative Examples 1 to 13 (Heartonic) In the composition of components shown below, the extract of the fusi prepared in Production Example 1 and hyaluronic acid having a molecular weight of 55,000 (hereinafter referred to as HA Abbreviated), hyaluronic acid having a molecular weight of 1,000,000 (hereinafter abbreviated as HAL), molecular weight 4,8
00 sodium hyaluronate (hereinafter abbreviated as HAN), dermatan sulfate having a molecular weight of 6,500 (hereinafter DS
), Potassium dermatan sulfate having a molecular weight of 7,000 (hereinafter abbreviated as DSK), chondroitin tetrasulfate having a molecular weight of 5,000 (hereinafter abbreviated as C4S), chondroitin tetrasulfate having a molecular weight of 30,000 (hereinafter, C4
SL), chondroitin 6-sulfate having a molecular weight of 5,500 (hereinafter abbreviated as C6S), molecular weight of 5,000
Chondroitin tetrasulfate L-lysine (hereinafter abbreviated as C4SL), heparin having a molecular weight of 4,300 (hereinafter, He
and a low molecular weight acidic mucopolysaccharide such as heparan sulfate having a molecular weight of 5,400 (hereinafter abbreviated as HS) are blended as shown in Table 1 and Table 2, respectively, to obtain respective hairnics. It was prepared and the above-mentioned tests were carried out.

(1) Composition Component content (wt%) ──────────────────────────────────── Ethanol 60.0 Bush extract, acidic mucopolysaccharides, etc. Described in Tables 1 and 2 Propylene glycol 1.0 Perfume 0.1 Purified water Remaining amount to make total amount 100.0 ────────── ───────────────────────────

(2) Preparation method The extract of Fuzi and the fragrance are dissolved in ethanol, the acidic mucopolysaccharides and propylene glycol are dissolved in purified water, and the respective solutions are uniformly mixed with stirring to obtain the respective hairnics. Was prepared.

(3) Characteristics As shown in Tables 1 and 2, the results obtained by conducting various tests of each Heartonic show the results of Comparative Examples 1 to 7 in which the low molecular weight acidic mucopolysaccharides of the present invention were blended alone, Comparative Examples 8 to 11 in which the extract alone is blended, or Comparative Examples 12 and 13 in which the high molecular weight acidic mucopolysaccharide and the extract of Fuzi are used in combination have a low hair growth promoting effect, and also have hair-raising and dandruff preventing effects. I was not able to admit. The hair nourishing agents of the present invention of Examples 1 to 10 in which the low molecular weight acidic mucopolysaccharide of the present invention and the extract of aconidium are mixed at the same time show a high hair growth promoting effect in mice and humans, and the results of practical tests are also good. It showed a good result. In Examples 1 to 10, skin irritation did not occur in various tests on human skin.

[0034]

[Table 1]

[0035]

[Table 2]

[0036]

EFFECTS OF THE INVENTION As described above, it is apparent that the present invention provides a hair nourishing agent which exhibits a remarkable hair growth promoting effect and is excellent in hair growth effect, hair loss prevention and dandruff prevention effect.

Claims (1)

[Claims] 1. A hair nourishing agent comprising at least one selected from the group of low-molecular-weight acidic mucopolysaccharides and salts thereof, and an extract of Fuzi.