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WO1993001294A1 - Enzyme derivee de plantes, sequences d'adn et leurs utilisations - Google Patents

Enzyme derivee de plantes, sequences d'adn et leurs utilisations Download PDF

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Publication number
WO1993001294A1
WO1993001294A1 PCT/GB1992/001187 GB9201187W WO9301294A1 WO 1993001294 A1 WO1993001294 A1 WO 1993001294A1 GB 9201187 W GB9201187 W GB 9201187W WO 9301294 A1 WO9301294 A1 WO 9301294A1
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Prior art keywords
gst
plant
subunit
sequence
promoter
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PCT/GB1992/001187
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English (en)
Inventor
Ian George Bridges
Simon William Jonathan Bright
Andrew James Greenland
David Charles Holt
Ian Jepson
Wolfgang Walter Schuch
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Zeneca Limited
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Priority to DE69232620T priority Critical patent/DE69232620T2/de
Priority to AT92913708T priority patent/ATE218163T1/de
Priority to DK92913708T priority patent/DK0603190T3/da
Priority to US08/170,294 priority patent/US5589614A/en
Priority to EP92913708A priority patent/EP0603190B1/fr
Priority to AU21959/92A priority patent/AU672362B2/en
Priority to CA002111983A priority patent/CA2111983C/fr
Priority to JP50206493A priority patent/JP3377526B2/ja
Publication of WO1993001294A1 publication Critical patent/WO1993001294A1/fr
Priority to US08/664,855 priority patent/US5866792A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088—Glutathione transferase (2.5.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Definitions

  • This invention relates to a glutathione-S- transferase enzyme and DNA sequences coding for it.
  • Glutathione-S-transferases are a family of enzymes which catalyse the conjugation of glutathione, via a sulphydryl group, to a large range of hydrophobic, electrophilic compounds. The conjugation can result in detoxification of these compounds and may result in their removal from tissue.
  • GST enzymes have been identified in a range of crop plants including maize, wheat, sorghum and peas. GST's comprise from 1 to 2% of the total soluble protein in etiolated maize seedlings.
  • GST-I Three isoforms of GST have been identified: GST-I, GST-II and GST-III.
  • the major isoform in maize tissue, GST-I is constitutively expressed and is capable of conjugating glutathione with pre-emergent herbicides such as alachlor.
  • GST-I and GST-II proteins have a native molecular weight of approximately 50 kD. As in mammals, maize GST's are dimeric; GST-I has
  • GST-II is a heterodimer of a 29 kD subunit identical to that found in GST-I
  • GST-II-29 and a novel 27 kD subunit (GST-II-27).
  • GST-II is detected at a very low basal level in the absence of safener, but its expression is enhanced dramatically by safener treatment. Like GST-I, GST-II confers resistance to certain herbicides.
  • GST-II is known to detoxify chloroacetanilide and thiocarbamate herbicides such as alachlor (Mozer et al, 1983, Biochemistry, 22:1068-1072).
  • a cDNA and a gene corresponding to the 29 kD subunit of GST-I have been cloned previously and sequenced (Wiegand et al, 1986, Plant Mol Biol, 7:235-243).
  • a cDNA corresponding to a 26 kD subunit of a third, minor component of GST activity in maize seedlings has been previously cloned and sequenced (Moore et al, 1986, Nucleic Acid Research, 18:7227-7235).
  • GST-III is a homodimer of these 26 kD subunits.
  • GST-III is constitutively expressed. It is known to detoxify herbicides such as
  • genomic DNA sequence encoding the gene promoter for the 27 kD subunit of the glutathione-S- transferase, isoform II, enzyme (GST-II-27), containing the nucleotide sequence shown in Figure 8 herewith and variants of the said sequence as permitted by the degeneracy of the genetic code.
  • NCIMB Marine Bacteria
  • 23 St Machar Drive 23 St Machar Drive
  • the invention also provides a GST-II-27 enzyme subunit having the amino acid sequence shown in Figure 4 herewith.
  • the invention further provides a cDNA sequence encoding this GST-II-27 subunit, having the nucleotide sequence shown in Figure 2 herewith and variants of the said sequence as permitted by the degeneracy of the genetic code.
  • the cDNA was deposited on 19 April 1991 in the National Collections of Industrial and Marine
  • NCIMB Bacteria
  • the cDNA for GST-II-27 has been utilised as a gene probe for the isolation of a corresponding genomic sequence which includes the promoter region.
  • the invention further provides a chemically switchable gene construct which includes the
  • GST-II-27 gene promoter operatively linked to a foreign gene or a series of foreign genes whereby expression of said foreign gene or said series of genes may be controlled by application of an effective exogenous inducer.
  • the invention also provides plants transformed with said gene
  • the GST-II-27 gene has been shown previously (International Application Number WO 90/08826) to be induced by certain chemical compounds, known as "herbicide safeners", which can be applied, as a spray, for example, to growing plants. Induction may be achieved by application of any suitable chemical including known safeners and other agrochemicals, chemical analogues and other potential inducers.
  • Such chemicals may include N,N-diallyl-2,2-dichloroacetamide (common name: dichloramid); 2,2,5-trimethyl-3-(dichloroacetyl)-1,3-oxazolidine; benzyl-2-chloro-4- (trifluoromethyl)-5-thiazole-carboxylate (common name: flurazole); naphthalene-1,8-dicarboxylic anhydride; 2-dichloromethyl-2-methyl-1,3-dioxolane;1-(dichloroacetyl)-hexahydro-3,3,8a-trimethyl
  • oxabetrinil cyometrinil; fenclorim; methoxyphenone.
  • the GST-II-27 promoter when linked to an exogenous or foreign gene and introduced into a plant by transformation, provides a means for the external regulation of expression of that foreign gene.
  • the foreign gene may be any gene other than the wild type GST-II-27 gene.
  • the inducible GST-II-27 promoter is functional in both monocotyledons and dicotyledons. It can therefore be used to control gene expression in a variety of genetically modified plants, including field crops such as canola, sunflower, tobacco, sugarbeet, cotton; cereals such as wheat, barley, rice, maize, sorghum; fruit such as tomatoes, mangoes, peaches, apples, pears, strawberries, bananas and melons; and vegetables such as carrot, lettuce, cabbage and onion.
  • the GST-II-27 promoter is also suitable for use in a variety of tissues, including roots, leaves, stems and reproductive tissues.
  • the GST-II-27 protein was extracted from the same type of tissue, purified and used to raise a sheep antiserum (characterised by western and dot blot analysis).
  • GST-II-27 genomic clones were isolated by screening a maize genomic library with a carefully designed probe prepared from the above cDNA. By mapping these clones, a fragment containing the promoter region was isolated and sequenced.
  • the promoter sequence was used to construct plant gene expression cassettes. These cassettes were introduced into both monocotyledons and dicotyledons and shown to control gene expression in an inducible manner.
  • the invention further provides a method to produce a herbicide resistant transgenic plant which comprises the incorporation of DNA encoding GST polypeptides into the plant such that a
  • the invention also provides herbicide resistant plants produced by said method, and their progeny.
  • a resistant plant is defined as one which displays enhanced tolerance to a herbicide when compared to a standard plant.
  • Resistance may vary from a slight increase in tolerance to the effects of the herbicide to total resistance where the plant is unaffected by the presence of herbicide.
  • the genetic sequence coding for a rat GST is disclosed and used to construct plant transformation vectors for tobacco; some
  • DNA encoding GST polypeptide subunits may be incorporated into a plant transformation vector under the control of a suitable promoter
  • a plant expressing GST-I 29 kD polypeptide subunits will show GST-I enzyme activity.
  • a plant co-expressing GST-I 29 kD subunits and GST-II 27 kD subunits will show GST-II enzyme activity, and some GST-I activity.
  • a plant expressing GST-III 26 kD subunits will show GST-III enzyme activity.
  • a plant co-expressing GST-I 29 kD and GST-III 26 kD subunits will show GST-I and GST-III activity.
  • a plant co-expressing GST-I 29 kD, GST-II 27 kD and GST-II 26 kD polypeptide will show GST-I, GST-II and GST-III activity. All such plants will be capable of conjugating glutathione with certain pre-emergent herbicides: they will thus be resistant to these herbicides.
  • GST activity is known to be effective against a range of chemical herbicides, including the
  • chloroacetanilides and the thiocarbamates may be active against other compounds which show herbicidal properties.
  • the actual spectrum of herbicide resistance displayed by the transgenic plant will depend on which GST isoform is active (GST-I, GST-II or GST-III) or on the relative activity of the various GST isoforms present in the plant.
  • GST-II is active against alachlor and acetochlor
  • GST-III is active against atrazine.
  • the method of producing herbicide resistant transgenic plants may be used not only to confer resistance on previously susceptible plants or to increase existing resistance, but also to broaden the range of herbicides to which the plant is resistant. So the ability to introduce multiple resistances by expressing the various GST isoforms within the transgenic plants is advantageous for agricultural purposes. Specific resistance to a particular herbicide may be achieved by
  • GST-II activity in the plant may provide resistance to certain herbicides which cannot be detoxified by the GST-I or GST-III enzymes.
  • GST-II activity via transformation with the GST-II-27 sequence
  • Plants may be transformed with constructs containing sequences which encode GST subunits according to a variety of known methods
  • transformed cells may then in suitable cases be regenerated into whole plants in which the new nuclear material is stably incorporated into the genome. Both transformed monocot and dicot plants may be obtained in this way, although the latter are usually more easy to regenerate.
  • genetically modified plants which may be produced include field crops such as canola, sunflower, tobacco, sugarbeet, cotton and cereals such as wheat, barley, rice, sorghum and also maize.
  • Some plant species do not naturally contain GST enzymes which can detoxify herbicides.
  • GST-II-27 sequence under control of a constitutive promoter as this will mean that the GST-II enzyme is constitutively expressed within the transgenic maize plant. This avoids the need to apply a chemical safener to the maize seed or plant. Normally, GST-II enzyme activity is inducible. It is current practice to apply a safener (such as dichloramid) as a seed coating to induce GST-II enzyme activity in the emerging plant, thus conferring herbicide resistance.
  • a safener such as dichloramid
  • transgenic plant will give a plant with GST-II activity.
  • DNA encoding the GST-I 29 kD subunit or the GST-II 27 kD subunit may be incorporated into a vector under the control of a suitable promoter such as the 35S CaMV promoter. Plants may be transformed with vectors containing either the GST-I-29 or the GST-II-27 expression cassette.
  • Transformants expressing the respective GST-II subunits may be crossed to produce progeny expressing both GST-I-29 and GST-II-27, resulting in a herbicide resistant phenotype.
  • each plant may be co-transformed with vectors containing the GST-I-29 expression cassette and with vectors containing the GST-II-27 expression cassette.
  • DNA encoding the GST-I 29 kD subunit and the GST-II 27 kD subunit may be included in a single plant transformation vector under the control of a single promoter.
  • Transformants expressing both GST-II subunits will show a herbicide resistant phenotype; transformants expressing only or.e of the respective GST-II subunits (29 kD or 27 kD) may be crossed to produce progeny expressing both subunits.
  • the above methods can be adapted to produce herbicide resistant plants expressing the GST-I enzyme (transformation with DNA encoding GST-I-29), the GST-II enzyme (transformation with DNA encoding GST-III-26, as isolated by Moore et al, 1986,
  • DNA encoding the GST subunits is introduced into the plant under control of a constitutive promoter (such as the 35S CaMV
  • DNA encoding the GST subunits may also be included in a plant transformation vector under the control of an inducible promoter, to give inducible herbicide resistance in the transgenic plants.
  • an inducible promoter includes the chemically-inducible
  • Resistance may be switched on by application of a suitable inducer (such as a chemical safener).
  • a suitable inducer such as a chemical safener
  • the ability to express or to increase herbicide resistance only when required may be advantageous. For example, during rotation of crops, individuals of the first crop species may grow the following year in the field to be
  • a herbicide may be used to destroy these un-induced and still susceptible “volunteer” plants.
  • Induction of GST expression only when herbicide resistance is required may also be metabolically more efficient in some circumstances as the plant is producing GST polypeptides only when required.
  • Figure 1 is a time course graph showing GST activity in induced and uninduced maize root tissue.
  • Figure 2 shows the nucleotide sequence of cDNA encoding GST-II-27.
  • Figure 3 shows the northern analysis of induced and uninduced RNA samples.
  • Figure 4 shows the amino acid sequence of GST-II-27 compared to the amino acid sequences of GST-I-29 and GST-III-26.
  • Figure 5 shows primer extension mapping of the genomic clones.
  • Figure 6 represents the 5' end of the
  • Figure 7 shows the strategy used to sequence 5.4 Kb of the GST-II-27 gene and promoter.
  • Figure 8 shows the nucleotide sequence of the GST-II-27 promoter.
  • FIG. 9 gives an overview of GST GUS vector construction.
  • FIG. 10 shows the maize transient
  • Figure 12 shows the maize stable
  • Figure 13 shows results of the protoplast transient expression assays.
  • FIG 14 shows results of the tobacco stable transformation experiments.
  • FIG. 15 shows results of the maize stable transformation experiments.
  • Figure 16 shows the tissue localisation of the GST II 27 kD subunit in untreated and safener treated maize.
  • Figure 17 shows GST II concentration following safener treatment of maize in field trials.
  • Figure 18 shows GST II concentration in variously-sized uninduced maize tassels compared to induced tissue.
  • Figure 19 illustrates preparation of the vectors containing the CaMV35S-GST-I-29 or
  • R-25788 For treatment of young maize seedlings, seeds were germinated on moist filter paper. After germination and growth (up to one week) the safener N,N-diallyl-2,2- dichloroacetamide (hereinafter referred to as R-25788) was added to the water in the filter paper and the seedlings grown for a further time period before harvesting of tissue.
  • Tissue was homogenised in 0.05M Tris. HCl, pH 7.8; 0.001M EDTA; 0.001M DTT; and 7.5%
  • Separation of the GST isoforms from the crude extract was achieved as follows: the crude extract was applied to a DEAE Sepharose column and washed with 0.01M Tris. HCl, pH 7.8; 0.001M EDTA; and 0.001M DTT. The bound GST was eluted with 0.3M potassium chloride. Fractions containing GST activity were combined and desalted using PD10 gel filtration columns. Separation of the GST I and GST II isoforms was achieved by FPLC on a mono-Q column and a zero to 0.4M potassium chloride concentration gradient.
  • Double-stranded cDNA was prepared from oligo dT-cellulose-purified RNA by a method employing RNase and E coli DNA polymerase I in the synthesis of the second strand, without prior purification of single-stranded cDNA (Gubler and Hoffman, 1983).
  • Lambda ZAP II was chosen as the cloning vector. GENERATION OF ANTIBODIES TO THE GST-II-27 ENZYME
  • detection system allowed detection of 0.1 ng of denatured GST-II-27.
  • the in vivo excision protocol (Stratagene) was carried out to liberate Bluescript phagemids.
  • Plasmid DNA prepared from four different lysates was designated pIJ13, pIJ15, pIJ17 and pIJ21 respectively.
  • Plasmid pIJ21 was deposited in the National Collections of Industrial and Marine Bacteria
  • NCIMB Aberdeen, with the accession number NCIMB 40413.
  • the 950 base pair insert from one of the clones was hybridised to a Southern blot of
  • the 950 base pair insert (the putative
  • GST-II-27 PCR product and the GST-I-29 specific oligo were also used to probe library filters of 1 ⁇ 10 6 recombinants. Different hybridisation patterns were obtained for each probe.
  • GST-III-26 at the 5' end is dissimilar to the 5' region of pIJ17KS, showing that the latter codes for a different protein.
  • pIJl7KS strongly hybridised to a 0.95 kB transcript in induced RNA (I) isolated from a range of treated tissues including roots (R), tassel (T), silks (S) and leaf (L). With uninduced RNA (U), there was a poor hybridisation signal or in some cases no signal, as shown in Figure 3.
  • the total amino acid composition of each subunit was determined using an Applied Biosystems 420AH Derivatizer Automated Amino Acid Analyser. This showed that there was close agreement between the measured composition and that predicted from the nucleic acid sequence for both the 29 kD and 27 kD subunits.
  • N-terminal sequence analysis was performed on each subunit by Edman Degradation using an Applied Biosystems 477 Pulsed Liquid Phase Automated Amino acid Sequence Analyser. This showed that the N- terminal sequence of the 29 kD subunit is identical to the 29 kD subunit of GST-I. The N-terminus of the GST-II 27 kD subunit was blocked to Edman
  • Each subunit was reduced using 6M Guanidine-HCL and 45mM Dithiothreitol at 50°C for 15 minutes and then alkylated with 8mM Iodoacetamide at 20°C for 15 minutes.
  • the protein was diluted to produce a 2M Guanidine-HCL solution and Endoproteinase Lysine C added (1:20 protease:GST). Protease digestion was performed overnight at 37°C (Stone et al; Chap. 2 in "A Practical Guide to Protein and Peptide
  • isolated cDNA clone corresponds to the GST-II-27 subunit.
  • Figure 4 compares the amino acid sequences of GST-II-27, GST-I-29 and GST-III-26.
  • An asterisk (*) indicates a position in the alignment is perfectly conserved; a dot (.) indicates a position is well conserved; a tick ( ⁇ ) indicates a position which is conserved with rat GSTs.
  • GST-ll-27 shows homology to the two known isoforms, GST-I-29 and GST-III-26.
  • GST-II-27 is 57% identical with
  • the cDNA for GST-II-27 was utilised to design a gene probe for the isolation of a corresponding genomic sequence which included the promoter region.
  • the GST-II-27 cDNA sequence was examined to identify a specific region which does not occur in other maize GSTs or other plant/animal/viral/vector sequences, and hence would be suitable as a
  • PCR probe was random prime labelled and used to screen 5 ⁇ 10 6 recombinants from a maize genomic library (partial Mbol digested DNA with an average insert size of 15kB).
  • the positive clones were plaque-purified with the 3' PCR probe and a 5' oligo (130 bp from the 5' end of the cDNA). These genomic clones were then mapped to identify fragments running from the 5' end of the cDNA into the promoter region. An EcoRI fragment containing around 4kB of promoter region was isolated. This fragment was subcloned into a pBS vector, designated plasmid pGIE7, which was deposited in the National Collections of Industrial and Marine Bacteria (NCIMB), Aberdeen, with the accession number NCIMB 40426.
  • NCIMB National Collections of Industrial and Marine Bacteria
  • Plasmid pGIE7 contains the EcoRI-EcoRI fragment which covers the GST-II-27 promoter region plus some coding
  • sequence The sequence of exon 1 (underlined) matches that of the 5' end of the cDNA.
  • Genomic subclones pGIE7 and pGIS15 were used to sequence 5.4 Kb of the GST-II-27 gene and promoter. The majority was sequenced on both strands according to the strategy illustrated in Figure 7.
  • Figure 8 shows the nucleotide sequence of 3.8 kB of the promoter sequence from the 5' EcoRI site to the predicted translation start point.
  • GUS vectors were constructed using approximately 3.8 Kb of the GST-II-27 promoter. Nde I was used to cut the GST-II-27 promoter at the ATG and 4 Kb upstream. This fragment was cut with EcoRI, blunted and cloned into Sma I site of pTAK (a Bin19 based promoterless GUS construct). The GST GUS cassette from pGSTTAK was then cloned into the transient assay vector pPUG (3.8 GST promoter and GUS). The same GST GUS cassette was also cloned into a pUC derived vector containing the Bar selectable cassette giving pZM/RMS-3.
  • Figure 9 gives an overview of the method of vector construction.
  • Figure 10 shows the final structure of the maize transient transformation vector, pPUG;
  • Figure 11 shows the tobacco stable transformation vector, pGSTTAK;
  • Figure 12 shows the maize stable transformation vector, pZM/RMS-3.
  • Protoplasts were isolated form maize cell suspension lines or from in vitro grown maize leaves by a standard enzymatic digestion, sieving and washing procedure. Transformation was
  • Bin19 vector pGSTTAK containing 3.8 Kb of the GST-II-27 promoter 5' to the GUS reporter gene and nos terminator was used to generate transgenic tobacco using Agrobacterium Ti plasmid technology as described by Bevan (1984, Nucleic Acids
  • the GST GUS vector RMS 3 was used to generate transgenic maize plants using particle bombardment (Gordon-Kamm et al, 1990, Plant Cell, 2:603-618). When leaf tissue was painted with herbicide safener as described above, inducible GUS activity was observed (as shown in Figure 15).
  • R-29148 (2,2,5-trimethyl-3-(dichloroacetyl)-1,3-oxazolidine) or formulation alone were applied as a root drench to maize plants (400 mg R-29148/plant) and after a period of time crude protein extracts were prepared from samples of mature and immature leaf, root, stem and tassel. Western blot analysis was carried out using these extracts and the anti-GST-II-27-sera or the anti-GST-I-29-sera. The results show that the 29 kD subunit (GST-I-29) is constitutively expressed in all the tissues tested and was inducible in all tissues by safener application. The 27 kD subunit, specific to GST-II, was only constitutively
  • Figure 16 shows the western blots allowing tissue localisation of the GST-II 27 kD subunit in untreated (control) and safener treated maize mature leaf (2), immature leaf (3), stem (4), root (5) and tassel (6).
  • the blots also include markers (lane 1) and pure GST-II (lane 7).
  • R- 25788 N,N-diallyl-2,2-dichloroacetamide
  • R-29148 (2,2,5-trimethyl-3-(dichloroacetyl)-1,3-oxazolidine
  • Safener was applied by a spray application using overhead spraying (30 cm above plants) at four application rates (three replications per rate) as follows:
  • the control treatments involved spraying
  • the tassels were harvested and split into three sizes: pre-meiotic, meiotic and post-meiotic floret tissue. Crude protein extracts were prepared from floret tissue and stored at -70°C.
  • the levels of GST-II induction was also determined by separating the GST isoforms by FPLC ion exchange chromatography and assaying for the presence of GST using CDNB as the substrate.
  • Figure 17 gives the overall mean values for treatment with R-25788 and
  • the nucleotide sequence of a cDNA encoding GST-I-29 was published by Wiegand et al. in Plant Mol Biol, 1986, 7:235-243. Using this sequence, a specific oligonucleotide probe was designed and used to isolate a full length cDNA (plasmid pIJ4) encoding GST-I-29 from a safener induced seedling root cDNA library. Isolation of the cDNA encoding GST-II-27 (as shown in Figure 2) has already been described. The full length cDNAs may be
  • FIG. 19 shows how these constructs may be prepared.
  • the full length GST-I-29 coding region and the full length GST-II-27 sequence are isolated from pIJ4 and from plJ21 respectively by digestion with EcoRI. These fragments are filled in using Klenow/T4 polymerase to produce blunt ends. The blunt-ended fragments are then ligated 3' to the 35S CaMV promoter of pJRIi at the Smal cloning site. Recombinants containing cDNA inserts in the correct orientation are be selected using
  • Tobacco plants may be transformed with vectors containing either the CaMV35S-GST-I-29 or the
  • Transformants expressing the respective GST-II subunits may be determined by Western blotting analysis with subunit specific antisera (as already described). Such transformants may be crossed to produce progeny expressing both GST-I-29 and GST-II-27, resulting in a herbicide resistant phenotype.

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Abstract

L'invention décrit la sous-unité de 27 kD induite chimiquement de l'enzyme glutathione-S-transférase, l'isoforme II (GST-II-27), ainsi que les séquences la codant. Elle décrit, en particulier, une séquence d'ADN génomique codant le promoteur de gènes de la sous-unité de GST-II-27. Quand il est relié à un gène exogène et introduit dans une plante par transformation, le promoteur de GST-II-27 fournit un moyen de régulation externe de l'expression de ce gène exogène. La transformation au moyen d'ADN codant des polypeptides de glutathione-S-transférase produit des plantes transgéniques à résistance aux herbicides.
PCT/GB1992/001187 1991-07-02 1992-07-01 Enzyme derivee de plantes, sequences d'adn et leurs utilisations WO1993001294A1 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DE69232620T DE69232620T2 (de) 1991-07-02 1992-07-01 Von pflanzen abgeleitetes enzym und dna-sequenzen und ihre verwendung
AT92913708T ATE218163T1 (de) 1991-07-02 1992-07-01 Von pflanzen abgeleitetes enzym und dna-sequenzen und ihre verwendung
DK92913708T DK0603190T3 (da) 1991-07-02 1992-07-01 Planteafledt enzym og DNA-sekvenser, og anvendelser deraf
US08/170,294 US5589614A (en) 1991-07-02 1992-07-01 Plant-derived glutathione-S-transferase isoform II promoter
EP92913708A EP0603190B1 (fr) 1991-07-02 1992-07-01 Enzyme derivee de plantes, sequences d'adn et leurs utilisations
AU21959/92A AU672362B2 (en) 1991-07-02 1992-07-01 Plant-derived enzyme and DNA sequences, and uses thereof
CA002111983A CA2111983C (fr) 1991-07-02 1992-07-01 Enzyme formee a partir d'un vegetal et sequences d'adn, et utilisations correspondantes
JP50206493A JP3377526B2 (ja) 1991-07-02 1992-07-01 植物由来の酵素及びｄｎａ配列並びにそれらの使用
US08/664,855 US5866792A (en) 1991-07-02 1996-06-17 Plant-derived enzyme and DNA sequences and uses thereof

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ATE218163T1 (de) 2002-06-15
JPH06511385A (ja) 1994-12-22
ES2178637T3 (es) 2003-01-01
DE69232620T2 (de) 2002-12-12
CA2111983C (fr) 2005-08-30
CA2111983A1 (fr) 1993-01-21
EP0603190B1 (fr) 2002-05-29
AU690855B2 (en) 1998-04-30
GB9114259D0 (en) 1991-08-21
DE69232620D1 (de) 2002-07-04
JP3377526B2 (ja) 2003-02-17
AU2195992A (en) 1993-02-11
AU6210496A (en) 1996-11-21
AU672362B2 (en) 1996-10-03
EP0603190A1 (fr) 1994-06-29
US5866792A (en) 1999-02-02
US5589614A (en) 1996-12-31
JP2003174891A (ja) 2003-06-24
DK0603190T3 (da) 2002-09-23

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