WO1996001320A3 - Complete genomic sequence of autographa californica nuclear polyhedrosis virus C6 - Google Patents
Complete genomic sequence of autographa californica nuclear polyhedrosis virus C6 Download PDFInfo
- Publication number
- WO1996001320A3 WO1996001320A3 PCT/IB1995/000578 IB9500578W WO9601320A3 WO 1996001320 A3 WO1996001320 A3 WO 1996001320A3 IB 9500578 W IB9500578 W IB 9500578W WO 9601320 A3 WO9601320 A3 WO 9601320A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- acnpv
- orfs
- genome
- virus
- nuclear polyhedrosis
- Prior art date
Links
- 241000201370 Autographa californica nucleopolyhedrovirus Species 0.000 title abstract 6
- 108700026244 Open Reading Frames Proteins 0.000 abstract 4
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 241000700605 Viruses Species 0.000 abstract 2
- 230000004543 DNA replication Effects 0.000 abstract 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 abstract 1
- 108020005202 Viral DNA Proteins 0.000 abstract 1
- 230000033228 biological regulation Effects 0.000 abstract 1
- 230000000977 initiatory effect Effects 0.000 abstract 1
- 229930182817 methionine Natural products 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 241000701447 unidentified baculovirus Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The complete nucleotide sequence of the genome of clone 6 of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) has been determined. The molecule comprises 133,894 base-pairs and has an overall A+T content of 59 %. Our analysis suggests that the virus encodes some 154 methionine-initiated, and potentially expressed, open reading frames (ORFs) of 150 nucleotides or greater. These ORFs are distributed evenly throughout the virus genome on either strand. The ORFs are arranged as adjacent, non-overlapping reading frames, separated by short intergenic regions. Based on the primary nucleotide sequence, predictions have been made concerning the functions of certain genes, the sites for initiation of viral DNA replication, the regulation of early and late gene transcription, and factors that may affect the AcNPV gene translational efficiency. The genome sequence data confirm, with minor differences, the information obtained for other AcNPV clones. It is proposed that clone C6 is considered the archetype AcNPV for comparison purposes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU28972/95A AU2897295A (en) | 1994-07-04 | 1995-06-30 | Autographa californica complete genome sequence |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9413420A GB9413420D0 (en) | 1994-07-04 | 1994-07-04 | Autographa californica nuclear polyhedrosis virus dna sequences |
| GB9413420.2 | 1994-07-04 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1996001320A2 WO1996001320A2 (en) | 1996-01-18 |
| WO1996001320A3 true WO1996001320A3 (en) | 1996-07-25 |
Family
ID=10757768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/IB1995/000578 WO1996001320A2 (en) | 1994-07-04 | 1995-06-30 | Complete genomic sequence of autographa californica nuclear polyhedrosis virus c6 |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU2897295A (en) |
| GB (1) | GB9413420D0 (en) |
| WO (1) | WO1996001320A2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPN570295A0 (en) * | 1995-09-29 | 1995-10-26 | Commonwealth Scientific And Industrial Research Organisation | Biologically active proteins of viral origin |
| WO1999033963A1 (en) | 1997-12-31 | 1999-07-08 | Chiron Corporation | Metastatic cancer regulated gene |
| AU775017B2 (en) * | 1998-07-21 | 2004-07-15 | Dow Agrosciences Llc | Antibody-mediated down-regulation of plant proteins |
| CN114317608B (en) * | 2020-12-28 | 2023-08-22 | 陕西杆粒生物科技有限公司 | Gene knockout type baculovirus expression vector |
| CN114058598B (en) * | 2021-11-04 | 2023-04-28 | 中国科学院精密测量科学与技术创新研究院 | Novel recombinant baculovirus genome insertion site and application thereof |
| CN118956967A (en) * | 2024-04-17 | 2024-11-15 | 和元生物技术（上海）股份有限公司 | Nucleic acid molecule, recombinant baculovirus containing the same and application thereof |
-
1994
- 1994-07-04 GB GB9413420A patent/GB9413420D0/en active Pending
-
1995
- 1995-06-30 WO PCT/IB1995/000578 patent/WO1996001320A2/en active Application Filing
- 1995-06-30 AU AU28972/95A patent/AU2897295A/en not_active Abandoned
Non-Patent Citations (4)
| Title |
|---|
| M. KOOL AND J.M. VLAK;: "The structural and functional organization of the autographa californica nuclear polyhedrosis virus genome", ARCH. VIROL., vol. 130, pages 1 - 16 * |
| M.D. AYRES ET AL.;: "The complete DNA sequence of Autographa californica nuclear polyhedrosis virus", VIROLOGY, vol. 202, pages 586 - 605 * |
| R.D. POSSEE ET AL.;: "Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4 kbp Eco RI-I and -R (polyhedrin gene) region", VIROLOGY, vol. 185, pages 229 - 241 * |
| S.C. BRAUNAGEL ET AL.;: "Sequence, genomic organization of the EcoRI-A fragment of Autographica californica nuclear polyhedrosis virus, and identification of a viral-encoded protein resembling the outer capsid protein VP8 of Rotavirus", VIROLOGY, vol. 191, pages 1003 - 1008 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB9413420D0 (en) | 1994-08-24 |
| WO1996001320A2 (en) | 1996-01-18 |
| AU2897295A (en) | 1996-01-25 |
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