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WO1996011565A1 - Technique de culture des champignons et analogue, et sac d'entreposage de recipient pour culture de champignons et analogue - Google Patents

Technique de culture des champignons et analogue, et sac d'entreposage de recipient pour culture de champignons et analogue Download PDF

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Publication number
WO1996011565A1
WO1996011565A1 PCT/JP1995/002112 JP9502112W WO9611565A1 WO 1996011565 A1 WO1996011565 A1 WO 1996011565A1 JP 9502112 W JP9502112 W JP 9502112W WO 9611565 A1 WO9611565 A1 WO 9611565A1
Authority
WO
WIPO (PCT)
Prior art keywords
culture
bag
medium
nonwoven fabric
mushroom
Prior art date
Application number
PCT/JP1995/002112
Other languages
English (en)
Japanese (ja)
Inventor
Toshiharu Itoh
Hirofumi Iwasaki
Original Assignee
Asahi Kasei Kogyo Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Kasei Kogyo Kabushiki Kaisha filed Critical Asahi Kasei Kogyo Kabushiki Kaisha
Publication of WO1996011565A1 publication Critical patent/WO1996011565A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/60—Cultivation rooms; Equipment therefor
    • A01G18/64—Cultivation containers; Lids therefor
    • A01G18/66—Cultivation bags

Definitions

  • the present invention relates to a method for artificially cultivating mushrooms and a gas-permeable seal used as an accessory for containers enclosing a culture medium.
  • fungi such as shiitake, shimeji, enokitake, nameko, hiratake, matake, and mushroom
  • pharmacological mushrooms such as mushroom (reishiba), artificially cultivating inoculum, mycelium culture and mushroom cultivation. It is harvested as mushrooms that have grown.
  • the intermediate inoculum and mycelium are inoculated on an appropriately selected medium. It is known that it is important to prevent the invasion of harmful fungi such as.
  • the essence of the cultivation of the edible mushroom mycelium is basically, as disclosed in Japanese Patent Application Laid-Open No. 58-28212, a method for synthesizing a medium for mycelium transplantation using a bag of synthetic resin such as polyethylene, polypropylene or the like. , And a sterilization filter is applied to the upper filling opening of the bag.After sterilization or sterilization, the sterilization filter is removed and the medium is inoculated with the inoculum and sterilized again. The bag is placed in the culture room with the filter attached, and cultured.
  • Japanese Patent Application Laid-Open No. 62-111030 discloses a culture container having a structure in which pores are provided at positions other than the filling portion of the culture container, and vent holes made of paper, nonwoven fabric, or the like are provided.
  • the problem of the invasive propagation of various bacteria in the container culture method is most often caused by the installation of a disinfecting filter at the filling port of the bag of the bag container.
  • a disinfection filter can be attached to the filling port using a paper disinfection filter, a rigid plastic tubular body, or a rigid plastic with a plurality of holes formed in the top surface.
  • a cylindrical body is passed through the outer periphery of the opening (entrance) of the bag using the cap, and the periphery is folded back from the inside to the outside of the cylindrical body, and a sterilizing filter is inserted into the opening.
  • the cap is pressed and fitted to the outer periphery of the cylindrical body while sandwiching the periphery of the folded bag and the periphery of the sterilization filter.
  • the periphery of the synthetic resin bag and the periphery of the sterilization filter sandwiched between the cap and the cylindrical body of the container and bag filled with the culture medium are inevitably mixed. It is inevitable that a small gap of about 0.1 to 1.0 mm will occur between both edges due to wrinkles. As a result, although the filling containers (entrances) of the bag containers are sealed with the sterilization filter, the invasion of various bacteria into the culture medium from the gaps has not been prevented. . Therefore, the sterilization filter by the bag container method Luther deposition is unavoidable unless an inoculation room and a culture room are installed in a sterile environment, which places a significant economic burden.
  • An object of the present invention is to reduce the invasion of various bacteria from the medium filling part or the ventilation window of the bag container in the container cultivation method of edible mushrooms, particularly the culture method of mushrooms using the bag container. It is.
  • An object of the present invention is to provide an economically advantageous means capable of preventing contamination of a culture medium with various bacteria by a simple method, particularly in the culture of bags of mushroom mycelium.
  • the present invention provides a simple method that can remarkably reduce the spread of invasion of various bacteria into a culture medium by means of light economic burden in all processes including culture of inoculum and culture of mycelium in an artificial culture method using a container of mushrooms.
  • the problem is basically that in a container culture of mushrooms, aeration of the mushroom culture medium is performed with a specific nonwoven fabric material, that is, an average apparent density of 0.05 to 0.5 gZcm 3 , and a gas permeability of 10 It is achieved by ⁇ 500c.
  • Roh cm culture method carried out through the non ⁇ showing a 2 ZSE c.
  • the air permeability of the nonwoven fabric used in the present invention is 10 to 500 cc_cm 2 / sec, preferably 30 to 300 ccZcm 2 / sec. If the air permeability is less than lOccZcm 2 / sec, it is difficult to maintain environmental conditions suitable for culturing mycelium, and the growth of mycelium is inhibited. On the other hand, if it exceeds SOOccZcm 2 / sec, it will be difficult to prevent bacterial contamination from surroundings.
  • the air permeability is a value measured using a JIS-L-1096 Frajur tester.
  • the average apparent density of the nonwoven fabric used in the present invention is 0.05 to 0.50 g / cm 3 , and preferably 0.10 to (!
  • the nonwoven fabric can be made by a single method such as a spunbond method, a melt blow method, a tow opening method, a dry short fiber method, or a combination of two or more methods.
  • the fibers used in the nonwoven fabric include synthetic fibers such as polyolefin, polyamide, polyester, and polyacrylonitrile, and natural fibers such as cotton.
  • the woven diameter of the fibers is 0.5 to 100%. , Preferably 1 to 80 cm. If the fiber diameter is less than 0.5, the air permeability becomes poor, while if it exceeds 100, the filter performance becomes poor.
  • the material and woven diameter of the constituent fibers must be reduced. It can be mixed or laminated, or imparted with antibacterial properties.
  • an antimicrobial property is imparted to a nonwoven fabric obtained by laminating a microfiber nonwoven fabric having a fiber diameter of 0.5 to 6 / zm and a synthetic fiber nonwoven fabric having a fiber diameter of 10 to 100 / m, and the nonwoven fabric forming the above filter material.
  • a nonwoven fabric obtained by laminating a microfiber nonwoven fabric having a fiber diameter of 0.5 to 6 / zm and a synthetic fiber nonwoven fabric having a fiber diameter of 10 to 100 / m, and the nonwoven fabric forming the above filter material.
  • non-woven fabric in which silver ions and silver-based inorganic antibacterial agents such as phosphates and zeolite are kneaded into a synthetic resin, or quaternary ammonium salts, organic nitrogen compounds, organic silicon compounds
  • quaternary ammonium salts organic nitrogen compounds
  • organic silicon compounds There is a method of attaching a quaternary ammonium salt, an aromatic halogen compound or the like to
  • Basis weight of the nonwoven fabric forming the filter one material 20 ⁇ SOOgZcm 3, preferably 40 ⁇ 150gZcm 3.
  • the aeration of the culture medium in the container may be performed by: (1) sealing the medium filling part of the culture container, preferably a bag container, or the additional vent of the container with the specific nonwoven fabric; Opening sealing method, or (2) It is preferable to carry out a storage bag sealing method for further storing the culture container in the storage bag made of the nonwoven fabric.
  • the method according to culture container opening sealing method thickness 0.10 ⁇ 2.0Mm, an average apparent density 0.05 ⁇ O.SgZcm 2, medium containers in breathability 10 ⁇ 500ccZcm 2 / se nonwoven Takashi ⁇ Ro Alternatively, cover and seal the ventilation (moisture-permeable) window provided separately from the medium filling port and attached to the containers.
  • the opening of the culture container has an average thickness of 0.10 to 2.0 mm
  • the edible mushrooms are cultured and covered with an unwoven cloth having an apparent density of 0.05 to 0.5 g / cm 3 and a permeability of 10 to 500 cc / cm 2 minutes. This is a very simple method to prevent breeding.
  • a preferred embodiment of the culture method of the present invention that uses the opening sealing method is a method of sealing the filling port (entrance) of containers.
  • the nonwoven fabric is placed between the cylindrical body and the cap.
  • the medium-filled container in the container is sealed with a nonwoven fabric by fixing it between small gaps.
  • the nonwoven fabric has flexibility and sublimeness that does not easily cause wrinkles.
  • the thickness of the nonwoven fabric is 0.10 to 2.00 mm, preferably 0.20 to 1.50 mm. If the thickness is less than 0.10 m, it will be insufficient to seal the small gap between the cylindrical body and the cap, and if the thickness exceeds 2.00 mm, the fitting between the cylindrical body and the cap will be insufficient. .
  • the cylindrical body and the cap attached to the container cap or bag is particularly excellent in flexibility.
  • the flexibility is less than 15cm, preferably 5 cn !, as measured by the 45 ° cantilever method of J1S-L-1906. ⁇ 13cm. If it is 15 cm or more, the cylindrical body and the cap will be insufficiently fitted due to small gaps due to sealing.
  • the shape and material of the container are not particularly limited as long as they can hold a medium suitable for the growth and physiology of mushrooms.
  • Examples of the material include polyethylene, polypropylene such as polypropylene, polyamide, and polyester.
  • the bag is a bag made of a transparent or translucent film made of polyethylene, polypropylene or the like. Further, a rigid plastic tubular body and a hard cap having a plurality of holes formed in the upper surface are attached to the inlet of the bag, or the nonwoven fabric is attached to an upper part of the bag.
  • the shape is round, square, rectangular, equal in size and shape is not particularly limited, but it is preferable to improve the ventilation of the bag body with oxygen gas, carbon dioxide gas and the like.
  • the nonwoven fabric of the present invention has different shapes, dimensions, etc. depending on the culture containers and bags, but in the cylindrical capping method described below, for example, A 10 cm square non-woven fabric is preferred.
  • the culture method is to prevent bacterial contamination after inoculation of the inoculum, and to sufficiently prevent bacterial contamination, the medium must be thoroughly sterilized. It goes without saying that care must be taken during the sterilization process, the cooling process, and the inoculation of the inoculum in an aseptic environment.
  • a simple closed chamber made of non-woven fabric having air permeability, water resistance, antibacterial properties, etc. is provided at the exit of the heat-killed sesame, and inoculation of inoculum is performed in the chamber.
  • At least some of the methods for storing culture containers in non-woven bags In addition, storage of a mushroom mycelium culture container or bag using a non-woven fabric with an average apparent density of 0.05 to 0.50 g Z cm 3 and air permeability of 10 to 500 cc / cm 2 / sec. This is achieved by using a bag.
  • the culture containers When the culture containers are stored in this storage bag, they can be used for cultivation indoors or in a house under an environment such as a sterile environment.
  • sterile environment includes, in its original sense, a sterile room and a sterile atmosphere environment where a commercially available bactericide such as a halogen-based, peroxide-based, or alcohol-based mist is sprayed.
  • a commercially available bactericide such as a halogen-based, peroxide-based, or alcohol-based mist is sprayed.
  • a commercially available bactericide such as a halogen-based, peroxide-based, or alcohol-based mist is sprayed.
  • the container it is possible to prevent germ contamination due to small gaps during culturing, especially in the initial stage, and thus high productivity and operability can be achieved without using an expensive sterile room during culturing. It can be cultivated indoors, in basements, in horror holes, and in houses and tents that are not directly exposed
  • the storage bag of the present invention is used for the main purposes of (1) improving the germ contamination prevention property, and (2) keeping the air permeability as large as possible. If the air permeability is poor, the amount of air required for the growth of mycelium cannot be maintained, and problems such as difficulty in adjusting the temperature of the culture medium in a high temperature environment such as summertime arise. Accordingly, the nonwoven fabric of the present invention, which has a large air permeability, a gap and a thickness that does not allow passage of various bacteria, is preferably used as a component of the storage bag.
  • the mushroom mycelium can be stored in the storage bag of the present invention one by one in a culture container or bag, or a large number of culture containers or bags can be stored in a room made of a nonwoven fabric and an impermeable material. Can be stored and cultured.
  • the container of the present invention can obtain the effect of preventing various bacteria even if a container for culture or a bag using an existing sterilization filter is stored.
  • the storage bag sealing method uses a storage bag to confine the aroma of cultured mushrooms and obtain edible mushrooms with an aroma similar to that of log cultivation. There is a clear effect.
  • the storage bag can be placed on a shelf or the like after storing the culture container or bag body, and can be cultured by a hanging method, which is a culture method excellent in workability and handleability.
  • the container uses the nonwoven fabric of the present invention, and a transparent synthetic resin film such as a polyolefin, a polyamide, or a polyester described later is used. At this time, in order to improve the adhesiveness, a heat sealing process and a composite film in which films having different melting points are laminated are used. The bonding may be performed using a thermocompression seal, a high-frequency seal, or an adhesive.
  • the storage bag opening is closed after storing the container or the bag.
  • the hermetically sealed state can be achieved by using, for example, heat bonding, a high-frequency seal, or an adhesive at the periphery of the opening of the storage bag.
  • the periphery of the opening is folded, and the folded portion is tied to a string or rubber band. Or a staple, a clip, etc., or a bendable material such as aluminum or copper, a round bar, a plate-like material, or a coil-like material near the opening. It can also be sealed by folding it on the upper part of the body and folding it three or four times with the core as the core, and then folding the folded part inward together with the core material.
  • the storage bag made of the nonwoven fabric of the present invention can prevent germ contamination and activate the mycelium in a favorable growth environment.
  • the purpose can be sufficiently achieved by double packaging the medium.
  • the moisture content of the bacterial bed and control of bacterial contamination can be controlled twice or three times.
  • the storage made of the nonwoven fabric of the present invention Enter the harvested bacterial bed directly into the bag
  • the moisture content preventing overdrying and creating a suitable humid environment
  • the contamination of various bacteria the mycelium can be proliferated and the mushrooms can be generated with high certainty.
  • FIG. 1 is an explanatory view of an embodiment of the present invention in which a culture medium of a mycelium is sealed, and an opening of a bag body is sealed with a non-woven fabric to culture edible mushrooms. Is shown.
  • FIG. 2 is an illustration of a method of cultivating edible mushrooms in which the culture bag enclosing the culture medium of the embodiment shown in FIG. 1 is further housed in the shrinkable bag of the present invention, and shows a longitudinal section.
  • FIG. 3 is a side view (A) and a perspective view (B) of the bag for mycelium culture of the present invention.
  • FIG. 4 is a side view (A), a perspective view (B), and a bottom view (C) of the bag for mycelium culture of the present invention.
  • FIG. 5 is a side view (A) and a perspective view (B) of the storage bag of the present invention. O Best mode for carrying out the invention
  • Containers have a moderate air permeability sufficient for cultivating mushroom mycelia and a sufficient amount for preventing invasion of various bacteria from the outside, since the specific nonwoven fabric is used in a part of the containers. Combines sterility.
  • Examples of the mode of sealing the opening of the container with a non-woven fabric include a mode used for a filling section used for inoculation of a medium filled with the medium in the container, and a mode in which the opening section is used. There is a mode in which it is fixed to the ventilation window added to a part of the outer containers.
  • FIG. 1 shows a culture method in which the inlet of the bag is sealed with the nonwoven fabric specified in the present invention.
  • reference numeral 1 denotes a bag, which is passed through the inside of a tubular body 3 at the neck and folded outward.
  • 4 is a cap, on the top of which a hole 5 for ventilation is provided.
  • the nonwoven fabric 2 of the present invention is hermetically sealed by being sandwiched between the lid member 4 and the container member folded outside the cylindrical body 3. Since the nonwoven fabric 2 has both sterility and air permeability, the sterility of the culture medium is maintained while supplying sufficient air to the mycelium in the culture medium 6.
  • the bag 20 or 30 is It comprises a part 22 or 32 and a part 21 or 31 composed of the nonwoven fabric of the present invention.
  • the openings 21 and 31 need to be used for portions that do not come into contact with the medium when the medium is filled.
  • FIG. 2 shows a state in which the bag shown in FIG. 1 is further stored in a storage bag 10 described later.
  • the culture vessel or bag is usually filled with 1.0 to 2.5 kg of medium and has a suitable weight.
  • the periphery of the opening can be folded back to the bottom surface of the culture vessel or bag, and the above-mentioned weight can be used to achieve a substantially sealed state.
  • a medium for culturing mushroom mycelium a commonly used medium can be used.
  • a mixture of rice bran, bran, corn powder, sawdust and a commercially available nutrient mixture mixed with about 65% by weight of water can be used.
  • Fig. 1 shows an example of a typical culture method.
  • the bag 1 is filled with the medium 6, and the nonwoven fabric 2 is attached to the bag opening 1 ′ .
  • a 9 cm square filter material 2 is formed into a tube.
  • the nonwoven fabric is adhered so as not to cause sealing when sandwiched between the tubular body and the cap. This is important for good control of the return air after sterilization or the entry of bacteria during cooling.
  • the cap 4 attached to the opening 1 ′ of the bag 1 and the nonwoven fabric 2 are removed, the seed medium is inoculated into the culture medium 6, and the nonwoven fabric 2 of the present invention is again covered with the cap.
  • This is a culture method in which 4 is press-fitted and sealed. Also, there is a culture method in which the nonwoven fabric is sandwiched between the opening of the container and the cap and pressed and fitted in the same manner as the bag body described above.
  • the culture can be performed in the same manner.
  • the storage bag of the present invention is used for the main purposes of (1) improving the germ contamination prevention property, and (2) keeping the air permeability as large as possible. Poor air permeability causes problems such as not being able to maintain the amount of air required for mycelial growth and making it difficult to adjust the temperature of the medium in a high-temperature environment such as in summer. Therefore, the storage bag shall be constituted.
  • the nonwoven fabric of the present invention which has a large air permeability and a gap and a thickness that is small enough to prevent passage of various bacteria, is preferably used.
  • the storage bag of the present invention uses the nonwoven fabric specified in the present invention in whole or in part.
  • the nonwoven fabric 41 of the present invention is provided in the ventilation portion, and the non-ventilated portion is transparent.
  • a bag-like material using a translucent synthetic resin film 42 may be used.
  • the shape of the container of the present invention is not particularly limited as long as the container or the bag for culture can be stored.
  • the inside of the container can be observed by using a transparent synthetic resin film in the air-impermeable part in FIG. It is preferable to set it.
  • the observation portion is preferably placed on the back side so as not to be exposed to direct sunlight except during observation, or may be covered with the nonwoven fabric of the present invention.
  • the storage bag of the present invention preferably uses at least 509 or more of the nonwoven fabric used in the present invention.
  • the mushroom mycelium can be stored in the storage bag of the present invention in a culture container or bag body one by one, or a large number of culture containers or bag bodies can be stored in a room made by using a nonwoven fabric and an impermeable material together. Can be stored and cultured.
  • the container of the present invention can obtain the effect of preventing various bacteria even if a container for culture or a bag using an existing sterilization filter is stored.
  • the storage bag can be placed on a shelf or the like after storing the culture container or bag body, and can be cultured by a hanging method, which is a culture method excellent in workability and handleability.
  • the container uses the nonwoven fabric of the present invention, and as shown in FIG. 5, a transparent synthetic resin film such as polyolefin, polyamide, or polyester is used. At this time, in order to improve the adhesiveness, a heat-sealing process and a composite film in which films having different melting points are laminated are used. As the bonding method, it is preferable to perform bonding using a thermocompression seal, a high-frequency seal, or an adhesive.
  • the storage bag opening is closed after storing the container or the bag.
  • the hermetically sealed state can be achieved by, for example, using heat bonding, a high-frequency seal, or an adhesive at the periphery of the opening of the storage bag.
  • the periphery of the opening is folded, and the folded portion is tied to a string or rubber band. Or a stapler, a crisp, or the like, or a bendable plate or coil made of a bendable material such as aluminum or copper, for example, stored near the opening. It can also be sealed by folding it on the upper part of the body and folding it three or four times with the core as the core, then folding the folded part inward together with the core material.
  • FIG. 2 shows a state where the bag body of FIG. 1 is stored in the storage bag 10.
  • the culture container or bag is filled with a medium of 1.0 to 2.5 kg of trade and has a suitable weight.
  • the edge of the opening located at the bottom can be folded back to the bottom surface of the culture vessel or bag, and the above-mentioned weight can be used to achieve a substantially sealed state.
  • the measuring method used in the present invention is as follows.
  • Synthetic resin bag Contents Polypropylene bag of 21 (width 140mm, height 330mm, fold-in 50mm cassette type) Medium: 5 parts rice bran (same weight or less) bran 30 parts, cone powder 10 Parts, 65 parts of sawdust mixed with 65 parts of water 1.2 kg (a cylindrical block with a diameter of 150 and a height of 130 mm)
  • Tubular body and cap made of hard plastic
  • FIG. 1 is a longitudinal sectional view schematically showing an implementation state of a culture bag using a nonwoven fabric 2 having a stiffness of 10.5 cm as shown in FIG.
  • Medium 6 has already been sterilized, cooled and inoculated with inoculum.
  • a cylindrical body 3 is passed around the periphery of the inlet 1 ′ of the culture bag 1, and the periphery of the bag inlet 1 ′ is folded from the inside to the outside of the cylindrical body 3. ing.
  • the nonwoven fabric 2 of the present invention is covered on the entrance 1 ′, and the periphery of the nonwoven fabric 2 is folded back and bent to the outer periphery. or
  • a cap 4 is pressed and fitted to the outer periphery of the tubular body 3 with the periphery of the nonwoven fabric sandwiched therebetween, and a plurality of holes 5 are formed in the upper surface of the cap.
  • a long-fiber non-woven fabric (ELTAS polypropylene (trade name, manufactured by Asahi Kasei Kogyo Co., Ltd.), a thickness of 0.50 mm, an average apparent density of 0.14 g Zcm 3 , 1 45ccZ cm 2 se c) bending resistance is a 9. 6 cm), polypropylene Fi Lum, made storage bag shown in Figure 5. It is a bag with a width of 30 cm and a height of 40 cm, and the film part is 100 cm wide and has a transparent impervious part with a height of 40 cm.100 pieces are prepared, and the temperature is 25-30 ° C.
  • the culture was performed in the same manner as in Example 1 except that the culture was performed in an outdoor tent for 30 days at a humidity of 30 to 80%. After the culture, the medium was inspected, and it was found that the mycelium of the mushroom was predominantly and well cultured in the medium.
  • Example 2 was carried out in the same manner as in Example 2 except that the culture period was changed from 30 days to 50 days instead of 30 days.
  • sanitizing filter made by Nippon Paper, craft paper, 0.10 mm thick 100 pieces were prepared by cutting 9 cm squares with an average apparent density of 0.60 g / cm 3 and air permeability of 1.2 cc Zcm 2 Xsec).
  • Example 1 was carried out in the same manner as in Example 1 except that the above-mentioned craft paper was used instead of the nonwoven fabric of the present invention.
  • the hard-crafted paper sanitizing filter is poor in flexibility and bulkiness, and has low air permeability. Therefore, when the filter is pressed and fitted between the tubular body and the cap, a shear occurs. However, it lacked airtightness and could not completely prevent invasion of various bacteria. After cultivation, the medium was examined, and found that 73 mediums were infested with various bacteria.
  • a commercially available sterilizing filter (Tyvek (registered trademark), manufactured by DuPont) having a thickness of 0.17 mm, an average apparent density of 0.35 g Zcm 3 , and an air permeability of l.Occ / cm 2 / sec or less is reduced to 9 cm square. Cut and prepared 100 pieces.
  • Example 1 was repeated in the same manner as in Example 1 except that the sterilizing filter (polyethylene nonwoven fabric) was used instead of the nonwoven fabric of the present invention.
  • the sterilizing filter polyethylene nonwoven fabric
  • Comparative Example 1 was carried out in the same manner as in Comparative Example 1, except that 50 cells were cultured for 60 days instead of 15 days.
  • Example 1 A bag of a commercially available sterilization filter (Comparative Example 1) was placed in the storage bag of Example 2, and the culture was performed in the same manner as in Example 2.
  • Example 4 was carried out in the same manner as in Example 2, except that the culture period was changed from 30 days to 50 days instead of 30 days.
  • Example 2 A bag of a commercially available sterilizing filter (Comparative Example 2) was placed in the storage bag of Example 2, and the culture was performed in the same manner as in Example 2.
  • the medium was examined. As a result, the mycelium of the mushroom in the medium was predominantly and well cultured. Good prevention of bacterial contamination
  • the bag shown in Fig. 3 made of polypropylene film (Height: 45cm x Width: 20cm, folded size: 6cm, Drilled 300 hole at the center of the bag, 15cm below the upper end of the entrance of the bag, Long-fiber non-woven fabric, Ertaspori polypropylene (made by Asahi Kasei Kogyo Co., Ltd., thickness: 0.46 mm, average apparent density: 0.1 l S g Z cm 3 , air permeability: I SSccZ cm 2 / sec, rigidity: 8.5 cm) was cut into a width of 6 cm and a length of 7 cm square, and was adhered with a heat-resistant adhesive around the 30- ⁇ hole of the bag to form a culture bag.
  • Polypropylene film Height: 45cm x Width: 20cm, folded size: 6cm, Drilled 300 hole at the center of the bag, 15cm below the upper end of the entrance of the bag, Long-fiber non-
  • Shiitake mushrooms were cultured in the same manner as described above. After inoculation, apply an 18 cm long, 1 cm wide, 0.8 mm thick aluminum plate to the entrance of the bag, fold the aluminum plate as a core, and close the entrance. The culture was performed in the same manner as in Example 1, except that the culture was stopped. After the culture, the medium was examined. As a result, it was found that the mycelium of the fungus was predominantly and well cultured in the medium.
  • the culture bag of Example 7 was placed in the storage bag of Example 2, and the culture was performed by the culture method of Example 2.

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

Technique de culture des champignons consistant à boucher à l'aide d'une feuille de non-tissé l'orifice de remplissage du milieu de culture et la fenêtre additionnelle de ventilation d'un récipient, pouvant être un sac, renfermant une culture de semences ou de mycélium. Ladite feuille présente une épaisseur comprise entre 0,10 et 2,00 mm, une masse volumique apparente comprise entre 0,05 et 0,50 g/cm3 et une perméabilité aux gaz comprise entre 10 et 500 cc/cm2/s. Ledit récipient est de plus placé lui-même pour la culture dans un sac en non-tissé pour empêcher efficacement au stade initial le développement de diverses bactéries, par une opération simple et sans devoir recourir à une chambre stérile.
PCT/JP1995/002112 1994-10-14 1995-10-13 Technique de culture des champignons et analogue, et sac d'entreposage de recipient pour culture de champignons et analogue WO1996011565A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP24956294 1994-10-14
JP6/249562 1994-10-14

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WO1996011565A1 true WO1996011565A1 (fr) 1996-04-25

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CN105875202A (zh) * 2016-06-13 2016-08-24 蔡志闯 一种用于培育食用菌的接种机构、菌种容器以及方法
CN109511459A (zh) * 2018-10-18 2019-03-26 河北中尔新材料有限公司 包菇片及其制备方法
CN109769592B (zh) * 2019-03-27 2021-06-25 儋州牧春绿色生态农业开发有限公司 一种灵芝的栽培方法

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EP3398429A4 (fr) * 2015-12-28 2019-11-13 Kirin Company, Limited Procédé destiné à la production en masse de plantes, installation de production en masse, et sac de culture utilisé dans ledit procédé et ladite installation
US20230080143A1 (en) * 2021-07-13 2023-03-16 Bio365 LLC Plant growing system and method

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