WO1996039101B1 - Compositions and methods for naturally secreted extracellular matrix - Google Patents
Compositions and methods for naturally secreted extracellular matrixInfo
- Publication number
- WO1996039101B1 WO1996039101B1 PCT/US1996/009482 US9609482W WO9639101B1 WO 1996039101 B1 WO1996039101 B1 WO 1996039101B1 US 9609482 W US9609482 W US 9609482W WO 9639101 B1 WO9639101 B1 WO 9639101B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- extracellular matrix
- cells
- naturally secreted
- framework
- composition according
- Prior art date
Links
- 210000002744 extracellular matrix Anatomy 0.000 title claims abstract 18
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 title claims abstract 17
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 title claims abstract 17
- 238000000034 method Methods 0.000 title claims abstract 12
- 239000000203 mixture Substances 0.000 title claims abstract 11
- 210000004027 cell Anatomy 0.000 claims abstract 9
- 210000001519 tissue Anatomy 0.000 claims abstract 8
- 230000007547 defect Effects 0.000 claims abstract 5
- -1 polypropylene Polymers 0.000 claims 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims 2
- 229920001661 Chitosan Polymers 0.000 claims 2
- 229920000742 Cotton Polymers 0.000 claims 2
- 229920002307 Dextran Polymers 0.000 claims 2
- 108010010803 Gelatin Proteins 0.000 claims 2
- 239000000020 Nitrocellulose Substances 0.000 claims 2
- 239000004952 Polyamide Substances 0.000 claims 2
- 239000004743 Polypropylene Substances 0.000 claims 2
- 239000004793 Polystyrene Substances 0.000 claims 2
- 239000002729 catgut Substances 0.000 claims 2
- 229920002678 cellulose Polymers 0.000 claims 2
- 239000001913 cellulose Substances 0.000 claims 2
- 210000001612 chondrocyte Anatomy 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 239000003937 drug carrier Substances 0.000 claims 2
- 210000002889 endothelial cell Anatomy 0.000 claims 2
- 210000002919 epithelial cell Anatomy 0.000 claims 2
- 210000002950 fibroblast Anatomy 0.000 claims 2
- 229920000159 gelatin Polymers 0.000 claims 2
- 239000008273 gelatin Substances 0.000 claims 2
- 235000019322 gelatine Nutrition 0.000 claims 2
- 235000011852 gelatine desserts Nutrition 0.000 claims 2
- 229920002674 hyaluronan Polymers 0.000 claims 2
- 229960003160 hyaluronic acid Drugs 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 229920001220 nitrocellulos Polymers 0.000 claims 2
- 210000004416 odontoblast Anatomy 0.000 claims 2
- 229920000058 polyacrylate Polymers 0.000 claims 2
- 229920002647 polyamide Polymers 0.000 claims 2
- 229920000515 polycarbonate Polymers 0.000 claims 2
- 239000004417 polycarbonate Substances 0.000 claims 2
- 229920000728 polyester Polymers 0.000 claims 2
- 229920001155 polypropylene Polymers 0.000 claims 2
- 229920002223 polystyrene Polymers 0.000 claims 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims 2
- 239000011148 porous material Substances 0.000 claims 2
- 230000002207 retinal effect Effects 0.000 claims 2
- 229920002554 vinyl polymer Polymers 0.000 claims 2
- 102000008186 Collagen Human genes 0.000 claims 1
- 108010035532 Collagen Proteins 0.000 claims 1
- 102000003839 Human Proteins Human genes 0.000 claims 1
- 108090000144 Human Proteins Proteins 0.000 claims 1
- 229920000954 Polyglycolide Polymers 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000010261 cell growth Effects 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 229920001436 collagen Polymers 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000002349 favourable effect Effects 0.000 claims 1
- 210000000963 osteoblast Anatomy 0.000 claims 1
- 239000004633 polyglycolic acid Substances 0.000 claims 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 claims 1
- 239000003356 suture material Substances 0.000 claims 1
Abstract
The present invention discloses compositions containing naturally secreted human extracellular matricies, the methods of making those matricies, and the method of using those matricies. The extracellular matrix is obtained by growing cells into a three-dimensional support structure so that the cells secrete an extracellular matrix. Next, the cells are killed and removed from the support structure leaving only the extracellular matrix on the support structure. The extracellular matrix can then be removed from the support, combined with a suitable pharmacologically acceptable carrier and injected into a tissue defect area in the skin of a patient.
Claims
1. A method for the production of a naturally secreted extracellular matrix coated three-dimensional framework comprising the steps of:
(a) culturing cells on a three-dimensional framework under conditions favorable for cellular growth for a pre-determined time period such that an extracellular matrix is secreted onto the inoculated framework creating a coated framework; (b) killing the cells and
(c) removing the cells and cellular debris.
2. A method according to claim 1 wherein the three-dimensional framework is a material selected from the group consisting of polyamide, polyester, polystyrene, polypropylene, polyacrylates, polyvinyl compounds,
polycarbonate, polytetrafluorethylene, thermanox,
nitrocellulose, cotton, polyglycolie acid, catgut suture material, cellulose, gelatin, chitosan, hyaluronic acid and dextran,
3. A method according to claim 1 or 2, wherein the three-dimensional framework has pore spaces of about 150μm to about 220μm.
4. The method according to any one of claims 1 to 3, wherein the extracellular matrix is secreted by tissue specific cells selected from the group consisting of
fibroblasts, osteoblasts, odontoblasts, chondrocytes, epithelial cells, smoothst-muscle cells, retinal cells, endothelial cells, stromal cells or combinations thereof.
5. A method for the production of a naturally secreted extracellular matrix comprising carrying out steps (a), (b) and (c) according to any one of claims 1 to 4 and then (d) collecting the extracellular matrix deposited on the coated framework.
6. A composition comprising a three-dimensional framework coated with a naturally secreted extracellular matrix composed of human proteins.
7. A composition according to claim 6, wherein the framework is made from a material selected from the group consisting of polyamide, polyester, polystyrene,
polypropylene, polyacrylates, polyvinyl compounds,
polycarbonate, polytetrafluorethylene, thermanox,
nitrocellulose, cotton, polyglycolic acid, catgut sutures, cellulose, gelatin, chitosan, hyaluronic acid and dextran.
8. A composition according to claim 6 or 7, wherein the three-dimensional framework has pore spaces of about 150μm to about 220μm.
9. A composition according to any one of claims 6 to 8, wherein the naturally secreted extracellular matrix is secreted by tissue specific ceils selected from the group consisting of fibroblasts, oεteoblasts, odontoblasts, chondrocytes, epithelial cells, smooth muscle cells, retinal cells, endothelial cells, stromal cells or combinations thereof.
10. An injectable naturally secreted extracellular matrix composition for the treatment of tissue defects comprising a human naturally secreted extracellular matrix and a pharmaceutically acceptable carrier.
11. A composition according to claim 10, wherein the naturally secreted extracellular matrix is produced by a method according to claim 5.
12. A composition according to claim 11 wherein naturally secreted extracellular matrices secreted by
different tissue or cell types are mixed between said step (d) and step (e), consisting in processing the collected extracellular matrix with a pharmaceutically acceptable carrier, such that ratios of collagen types I-V, respective to each other, are adjusted.
13. A method for the repair of tissue defects comprising injecting at a site of a tissue defect a
composition according to any one of claims 10-12.
14. A method according to claim 13 wherein the naturally secreted extracellular matrix is produced by a method according to claim 5.
15. The use of a composition according to any one of claims 10-12 for the repair of tissue defects.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU62609/96A AU708090B2 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
| EP96921369A EP0967934B1 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
| NZ311313A NZ311313A (en) | 1995-06-06 | 1996-06-06 | Composition and method for making compositions for treatment of wrinkles and scars using human extracellular matrix |
| AT96921369T ATE290350T1 (en) | 1995-06-06 | 1996-06-06 | COMPOSITIONS AND METHODS RELATING TO A NATURALLY SECRETED MATRIX |
| DE69634454T DE69634454T2 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods relating to a naturally secreted matrix |
| JP50179097A JP3709487B2 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US470,101 | 1995-06-06 | ||
| US08/470,101 US5830708A (en) | 1995-06-06 | 1995-06-06 | Methods for production of a naturally secreted extracellular matrix |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1996039101A1 WO1996039101A1 (en) | 1996-12-12 |
| WO1996039101B1 true WO1996039101B1 (en) | 1997-01-09 |
Family
ID=23866272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/009482 WO1996039101A1 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5830708A (en) |
| EP (1) | EP0967934B1 (en) |
| JP (1) | JP3709487B2 (en) |
| KR (1) | KR19990022216A (en) |
| AT (1) | ATE290350T1 (en) |
| AU (1) | AU708090B2 (en) |
| CA (1) | CA2223892A1 (en) |
| DE (1) | DE69634454T2 (en) |
| ES (1) | ES2238076T3 (en) |
| NZ (1) | NZ311313A (en) |
| WO (1) | WO1996039101A1 (en) |
Families Citing this family (119)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6974571B2 (en) * | 1995-03-28 | 2005-12-13 | Thomas Jefferson University | Isolated stromal cells and methods of using the same |
| US6284284B1 (en) * | 1995-06-06 | 2001-09-04 | Advanced Tissue Sciences, Inc. | Compositions and methods for production and use of an injectable naturally secreted extracellular matrix |
| US7767452B2 (en) | 1997-02-20 | 2010-08-03 | Kleinsek Don A | Tissue treatments with adipocyte cells |
| WO1998036704A1 (en) * | 1997-02-20 | 1998-08-27 | Keller Gregory S | Augmentation and repair of dermal, subcutaneous, and vocal cord tissue defects |
| GB9720987D0 (en) * | 1997-10-01 | 1997-12-03 | Biocure Ltd | A multicellular in vitro assay of angiogenesis |
| US6225118B1 (en) | 1997-10-01 | 2001-05-01 | Biocure Limited | Multicellular in vitro assay of angiogenesis |
| US6660301B1 (en) | 1998-03-06 | 2003-12-09 | Biosphere Medical, Inc. | Injectable microspheres for dermal augmentation and tissue bulking |
| WO1999047186A1 (en) * | 1998-03-18 | 1999-09-23 | University Of Pittsburgh | Chitosan-based composite materials containing glycosaminoglycan for cartilage repair |
| US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
| US6428978B1 (en) * | 1998-05-08 | 2002-08-06 | Cohesion Technologies, Inc. | Methods for the production of gelatin and full-length triple helical collagen in recombinant cells |
| EP2295540A1 (en) * | 1998-11-19 | 2011-03-16 | Organogenesis, Inc. | Bioengineered tissue constructs and methods for producing and using them |
| US7615373B2 (en) | 1999-02-25 | 2009-11-10 | Virginia Commonwealth University Intellectual Property Foundation | Electroprocessed collagen and tissue engineering |
| US6662805B2 (en) | 1999-03-24 | 2003-12-16 | The Johns Hopkins University | Method for composite cell-based implants |
| US20030007954A1 (en) | 1999-04-12 | 2003-01-09 | Gail K. Naughton | Methods for using a three-dimensional stromal tissue to promote angiogenesis |
| US6372494B1 (en) | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| WO2001003750A1 (en) * | 1999-07-09 | 2001-01-18 | Advanced Tissue Sciences, Inc. | Human naturally secreted extracellular matrix-coated device |
| CA2383398C (en) * | 1999-08-23 | 2013-06-11 | Organogenesis, Inc. | Skin care compositions and treatments |
| AU1472501A (en) * | 1999-11-05 | 2001-05-14 | Gerigene Medical Corporation | Augmentation and repair of age-related soft tissue defects |
| US20080286242A2 (en) * | 1999-11-05 | 2008-11-20 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including mesenchymal cells |
| US20090074729A2 (en) * | 1999-11-05 | 2009-03-19 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including fibroblasts |
| US20080267923A2 (en) * | 1999-11-05 | 2008-10-30 | Donald Kleinsek | Hair undifferentiated cells |
| US7799325B2 (en) * | 1999-11-05 | 2010-09-21 | Kleinsek Donald A | Removal of hypertrophic scars |
| EP1259634A4 (en) | 2000-03-02 | 2004-07-07 | Univ Rochester | EX VIVO CREATION OF FUNCTIONAL LEUKEMIA CELLS IN A THREE-DIMENSIONAL BIOREACTOR |
| US6436424B1 (en) * | 2000-03-20 | 2002-08-20 | Biosphere Medical, Inc. | Injectable and swellable microspheres for dermal augmentation |
| ATE327783T1 (en) | 2000-03-20 | 2006-06-15 | Biosphere Medical Inc | INJECTABLE MICROBEADS FOR TISSUE BUILDING |
| US7338657B2 (en) * | 2001-03-15 | 2008-03-04 | Biosphere Medical, Inc. | Injectable microspheres for tissue construction |
| WO2001070289A2 (en) | 2000-03-20 | 2001-09-27 | Biosphere Medical, Inc. | Injectable and swellable microspheres for tissue bulking |
| US8366787B2 (en) * | 2000-08-04 | 2013-02-05 | Depuy Products, Inc. | Hybrid biologic-synthetic bioabsorbable scaffolds |
| US6638312B2 (en) | 2000-08-04 | 2003-10-28 | Depuy Orthopaedics, Inc. | Reinforced small intestinal submucosa (SIS) |
| EP1315796B1 (en) * | 2000-08-16 | 2006-07-12 | Duke University | Decellularized tissue engineered constructs and tissues |
| JP2004508305A (en) | 2000-09-01 | 2004-03-18 | ヴァージニア コモンウェルス ユニバーシティ インテレクチュアル プロパティー ファンデーション | Matrix and tissue based on electroprocessed fibrin |
| US20090130066A1 (en) * | 2000-11-06 | 2009-05-21 | Gerigene Medical Corporation | Augmentation and repair of sphincter defects with cells including muscle cells |
| ATE402002T1 (en) * | 2000-11-17 | 2008-08-15 | Univ Virginia Commonwealth | ELECTROPROCESSED COLLAGEN |
| AU2002220209B2 (en) | 2000-12-06 | 2006-05-25 | Robert J. Hariri | Method of collecting placental stem cells |
| US7311905B2 (en) | 2002-02-13 | 2007-12-25 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells |
| US7041868B2 (en) | 2000-12-29 | 2006-05-09 | Kimberly-Clark Worldwide, Inc. | Bioabsorbable wound dressing |
| EP2316919B1 (en) | 2001-02-14 | 2015-10-07 | Anthrogenesis Corporation | Post-partum mammalian placenta, its use and placental stem cells therefrom |
| CA2796875A1 (en) | 2001-02-14 | 2002-08-22 | Anthrogenesis Corporation | Post-partum mammalian placenta, its use and placental stem cells therefrom |
| US7163563B2 (en) | 2001-07-16 | 2007-01-16 | Depuy Products, Inc. | Unitary surgical device and method |
| WO2003007879A2 (en) * | 2001-07-16 | 2003-01-30 | Depuy Products, Inc. | Cartilage repair and regeneration scaffold and method |
| US7819918B2 (en) * | 2001-07-16 | 2010-10-26 | Depuy Products, Inc. | Implantable tissue repair device |
| JP4294474B2 (en) | 2001-07-16 | 2009-07-15 | デピュイ・プロダクツ・インコーポレイテッド | Meniscus reproduction device |
| DE60239342D1 (en) * | 2001-07-16 | 2011-04-14 | Depuy Products Inc | DEVICE FOR THE REPAIR OF CARTILING MATERIAL |
| WO2003007790A2 (en) | 2001-07-16 | 2003-01-30 | Depuy Products, Inc. | Hybrid biologic/synthetic porous extracellular matrix scaffolds |
| EP1416866A4 (en) * | 2001-07-16 | 2007-04-18 | Depuy Products Inc | Devices form naturally occurring biologically derived |
| US8025896B2 (en) | 2001-07-16 | 2011-09-27 | Depuy Products, Inc. | Porous extracellular matrix scaffold and method |
| WO2003007786A2 (en) | 2001-07-16 | 2003-01-30 | Depuy Products, Inc. | Porous delivery scaffold and method |
| EP2292091A1 (en) | 2002-02-13 | 2011-03-09 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta and uses and methods of treatment using said cells |
| WO2003072128A2 (en) * | 2002-02-22 | 2003-09-04 | Ebi, L.P. | Methods and compositions for treating bone or cartilage defects |
| US7498171B2 (en) | 2002-04-12 | 2009-03-03 | Anthrogenesis Corporation | Modulation of stem and progenitor cell differentiation, assays, and uses thereof |
| KR100527623B1 (en) * | 2002-06-01 | 2005-11-15 | 라이프코드인터내셔날 주식회사 | Biodegradable polymer scaffold containing extracellular matrix used for artificial organs and method for preparing same |
| MXPA05005673A (en) | 2002-11-26 | 2005-11-23 | Anthrogenesis Corp | Cytotherapeutics, cytotherapeutic units and methods for treatments using them. |
| IL156945A0 (en) * | 2003-07-15 | 2004-02-08 | Itzhak Tavori | Device and a method for orthopedic delivery of bone reconstruction medium |
| US7326571B2 (en) * | 2003-07-17 | 2008-02-05 | Boston Scientific Scimed, Inc. | Decellularized bone marrow extracellular matrix |
| JP2005211099A (en) * | 2004-01-27 | 2005-08-11 | Olympus Corp | Artificial organ component, manufacturing method of artificial organ formation and artificial organ |
| US7569233B2 (en) | 2004-05-04 | 2009-08-04 | Depuy Products, Inc. | Hybrid biologic-synthetic bioabsorbable scaffolds |
| CA2566765C (en) * | 2004-05-11 | 2011-08-23 | Synthasome Inc. | Hybrid tissue scaffolds containing extracellular matrix |
| US20070190108A1 (en) * | 2004-05-17 | 2007-08-16 | Arindam Datta | High performance reticulated elastomeric matrix preparation, properties, reinforcement, and use in surgical devices, tissue augmentation and/or tissue repair |
| CA2578951A1 (en) * | 2004-08-30 | 2006-03-09 | Theregen, Inc. | Cultured three dimensional tissues and uses thereof |
| US7513866B2 (en) | 2004-10-29 | 2009-04-07 | Depuy Products, Inc. | Intestine processing device and associated method |
| US7354627B2 (en) | 2004-12-22 | 2008-04-08 | Depuy Products, Inc. | Method for organizing the assembly of collagen fibers and compositions formed therefrom |
| CA2612188A1 (en) * | 2005-06-17 | 2007-01-04 | Theregen, Inc. | Methods for treating ischemic tissue |
| US7595062B2 (en) | 2005-07-28 | 2009-09-29 | Depuy Products, Inc. | Joint resurfacing orthopaedic implant and associated method |
| US20070065415A1 (en) * | 2005-09-16 | 2007-03-22 | Kleinsek Donald A | Compositions and methods for the augmentation and repair of defects in tissue |
| SI1957633T1 (en) | 2005-10-13 | 2014-04-30 | Anthrogenesis Corporation | Immunomodulation using placental stem cells |
| WO2007064305A1 (en) * | 2005-12-01 | 2007-06-07 | Agency For Science, Technology And Research | Three-dimensional reconstituted extracellular matrices as scaffolds for tissue engineering |
| JP2009521930A (en) | 2005-12-29 | 2009-06-11 | アントフロゲネシス コーポレーション | Co-culture of placental stem cells and stem cells derived from a second source |
| KR101900809B1 (en) | 2005-12-29 | 2018-09-20 | 안트로제네시스 코포레이션 | Placental stem cell populations |
| US20070178137A1 (en) * | 2006-02-01 | 2007-08-02 | Toby Freyman | Local control of inflammation |
| ES2709205T3 (en) | 2006-10-23 | 2019-04-15 | Celularity Inc | Methods and compositions for the treatment of bone defects with populations of placental cells |
| US7871440B2 (en) | 2006-12-11 | 2011-01-18 | Depuy Products, Inc. | Unitary surgical device and method |
| MX2009008559A (en) | 2007-02-12 | 2009-08-21 | Anthrogenesis Corp | Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations. |
| NZ612888A (en) | 2007-02-12 | 2015-02-27 | Anthrogenesis Corp | Treatment of inflammatory diseases using placental stem cells |
| US9200253B1 (en) | 2007-08-06 | 2015-12-01 | Anthrogenesis Corporation | Method of producing erythrocytes |
| CA2700617C (en) | 2007-09-28 | 2018-11-06 | Celgene Cellular Therapeutics | Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells |
| WO2009061447A2 (en) | 2007-11-07 | 2009-05-14 | Anthrogenesis Corporation | Treatment of premature birth complications |
| MX2010000394A (en) | 2007-11-30 | 2010-05-13 | Rnl Bio Co Ltd | Cellular therapeutic agent for incontinence of urine comprising stem cells originated from decidua or adipose. |
| WO2009073178A1 (en) | 2007-12-03 | 2009-06-11 | Sanbio, Inc. | Extracellular matrix from pluripotent cells |
| KR101650957B1 (en) | 2008-01-30 | 2016-08-24 | 히스토젠, 인코포레이티드 | Extracellular matrix compositions |
| EP2740498A3 (en) * | 2008-07-02 | 2014-09-10 | Allergan, Inc. | Compositions and methods for tissue filling and regeneration |
| NZ591292A (en) | 2008-08-20 | 2012-10-26 | Anthrogenesis Corp | Improved cell composition and methods of making the same |
| EP2365815B1 (en) | 2008-11-14 | 2019-09-18 | Histogen, Inc. | Extracellular matrix compositions for the treatment of cancer |
| JP5869342B2 (en) | 2008-11-19 | 2016-02-24 | アンスロジェネシス コーポレーション | Amnion-derived adherent cells |
| AU2010271212B2 (en) | 2009-07-10 | 2015-07-16 | Histogen, Inc. | Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions |
| WO2011084925A2 (en) | 2010-01-05 | 2011-07-14 | Cell Constructs, Inc. | Biomaterials made from human hair |
| DK3284818T3 (en) | 2010-01-26 | 2022-06-20 | Celularity Inc | Treatment of bone-related cancer using placenta stem cells |
| NZ602808A (en) | 2010-04-07 | 2014-10-31 | Anthrogenesis Corp | Angiogenesis using placental stem cells |
| KR20180099915A (en) | 2010-07-13 | 2018-09-05 | 안트로제네시스 코포레이션 | Methods of generating natural killer cells |
| CN103830383A (en) | 2010-11-12 | 2014-06-04 | 阿勒根公司 | Metabolized conditioned growth medium and methods of use |
| AR084754A1 (en) | 2010-12-30 | 2013-06-05 | Anthrogenesis Corp | COMPOSITIONS THAT INCLUDE MOTHER PLACENTA CELLS AND PLASMA RICH IN PLATES, AND THEIR METHODS OF USE |
| AR084753A1 (en) | 2010-12-30 | 2013-06-05 | Anthrogenesis Corp | COMPOSITIONS THAT INCLUDE ADHERENT CELLS DERIVED FROM AMNIOTIC SACO (AMDAC) AND PLASMA RICH IN PLATES (PRP) |
| AR093183A1 (en) | 2010-12-31 | 2015-05-27 | Anthrogenesis Corp | INCREASE IN THE POWER OF PLACENTA MOTHER CELLS USING MODULATING RNA MOLECULES |
| CA2822689C (en) | 2011-01-06 | 2015-12-29 | Humacyte | Tissue-engineered constructs |
| PL2714059T3 (en) | 2011-06-01 | 2019-04-30 | Celularity Inc | Treatment of pain using placental stem cells |
| WO2013055995A2 (en) | 2011-10-14 | 2013-04-18 | President And Fellows Of Harvard College | Sequencing by structure assembly |
| CA2859761C (en) | 2011-12-22 | 2023-06-20 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
| ES2991004T3 (en) | 2011-12-22 | 2024-12-02 | Harvard College | Methods for the detection of analytes |
| EP2847700B1 (en) * | 2012-05-09 | 2022-12-07 | Koninklijke Philips N.V. | Interventional information brokering medical tracking interface |
| WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
| EP2953635A4 (en) | 2013-02-05 | 2016-10-26 | Anthrogenesis Corp | NATURAL KILLER CELLS FROM PLACENTA |
| EP2971184B1 (en) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| AU2014274939B2 (en) | 2013-06-04 | 2020-03-19 | President And Fellows Of Harvard College | RNA-guideded transcriptional regulation |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| EP3371329A4 (en) | 2015-11-03 | 2019-06-19 | President and Fellows of Harvard College | METHOD AND APPARATUS FOR VOLUMETRIC IMAGING OF THREE DIMENSIONAL MATRIX CONTAINING NUCLEIC ACIDS |
| AU2017257624B2 (en) | 2016-04-25 | 2023-05-25 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| CN105920679B (en) * | 2016-04-26 | 2018-10-19 | 青岛大学 | A kind of preparation method of the dermal scaffold material with three-dimensional gradient pore structure |
| GB2569252A (en) | 2016-08-31 | 2019-06-12 | Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| GB2570412A (en) | 2016-08-31 | 2019-07-24 | Harvard College | Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing |
| WO2018191672A1 (en) * | 2017-04-14 | 2018-10-18 | President And Fellows Of Harvard College | Methods for generation of cell-derived microfilament network |
| US11718829B2 (en) | 2017-07-28 | 2023-08-08 | Breakthrough Tech Llc | Methods and compositions for manufacturing extracellular matrix |
| WO2019152522A1 (en) | 2018-01-30 | 2019-08-08 | Histogen, Inc. | Extracellular vesicles derived from cells cultured under hypoxic conditions and uses thereof |
| SG11202101934SA (en) | 2018-07-30 | 2021-03-30 | Readcoor Llc | Methods and systems for sample processing or analysis |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN111084788A (en) * | 2018-10-22 | 2020-05-01 | 上海蓝盎电子科技发展有限公司 | Artificial intercellular substance |
| US12077780B2 (en) | 2020-02-14 | 2024-09-03 | Allergan Sales, Llc | Conditioned medium from cells cultured under hypoxic conditions and uses thereof |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| EP4395796A4 (en) | 2021-09-01 | 2024-12-25 | Shanghai Qisheng Biological Preparation Co., Ltd. | CARTILAGE REGENERATION USING IN SITU POLYMERIZABLE INJECTABLE COLLAGEN COMPOSITIONS CONTAINING CHONDROCYTES OR STEM CELLS |
| EP4457266A1 (en) | 2021-12-28 | 2024-11-06 | Shanghai Qisheng Biological Preparation Co., Ltd. | Hyaluronic acid-collagen copolymer compositions and medical applications thereof |
| CN115006597B (en) * | 2022-06-02 | 2024-01-19 | 上海威高医疗技术发展有限公司 | Oral cavity repairing film and preparation method thereof |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3949073A (en) * | 1974-11-18 | 1976-04-06 | The Board Of Trustees Of Leland Stanford Junior University | Process for augmenting connective mammalian tissue with in situ polymerizable native collagen solution |
| US4424208A (en) * | 1982-01-11 | 1984-01-03 | Collagen Corporation | Collagen implant material and method for augmenting soft tissue |
| US4582640A (en) * | 1982-03-08 | 1986-04-15 | Collagen Corporation | Injectable cross-linked collagen implant material |
| US4837285A (en) * | 1984-03-27 | 1989-06-06 | Medimatrix | Collagen matrix beads for soft tissue repair |
| US5288617A (en) * | 1984-10-31 | 1994-02-22 | Commonwealth Scientific And Industrial Research Organization | Method of producing an antigenic preparation |
| US5460939A (en) * | 1986-04-18 | 1995-10-24 | Advanced Tissue Sciences, Inc. | Temporary living skin replacement |
| US5160490A (en) * | 1986-04-18 | 1992-11-03 | Marrow-Tech Incorporated | Three-dimensional cell and tissue culture apparatus |
| US5032508A (en) * | 1988-09-08 | 1991-07-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US5266480A (en) * | 1986-04-18 | 1993-11-30 | Advanced Tissue Sciences, Inc. | Three-dimensional skin culture system |
| US4721096A (en) * | 1986-04-18 | 1988-01-26 | Marrow-Tech Incorporated | Process for replicating bone marrow in vitro and using the same |
| US4963489A (en) * | 1987-04-14 | 1990-10-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US4803075A (en) * | 1986-06-25 | 1989-02-07 | Collagen Corporation | Injectable implant composition having improved intrudability |
| JPH041118Y2 (en) * | 1986-07-31 | 1992-01-14 | ||
| GB8708009D0 (en) * | 1987-04-03 | 1987-05-07 | Clayton Found Res | Injectable soft tissue augmentation materials |
| US5342765A (en) * | 1991-08-01 | 1994-08-30 | Occidental Chemical Corporation | Method of producing extracellular products from aerobic microorganisms |
| US5204382A (en) * | 1992-02-28 | 1993-04-20 | Collagen Corporation | Injectable ceramic compositions and methods for their preparation and use |
| US5478739A (en) * | 1992-10-23 | 1995-12-26 | Advanced Tissue Sciences, Inc. | Three-dimensional stromal cell and tissue culture system |
| US5541106A (en) * | 1993-04-05 | 1996-07-30 | Desmos, Inc. | Cell matrix stimulated attachment and hemidesmosome assembly |
| US5397352A (en) * | 1993-08-27 | 1995-03-14 | Burres; Steven | Method of recollagenation |
| US5516532A (en) * | 1994-08-05 | 1996-05-14 | Children's Medical Center Corporation | Injectable non-immunogenic cartilage and bone preparation |
-
1995
- 1995-06-06 US US08/470,101 patent/US5830708A/en not_active Expired - Lifetime
-
1996
- 1996-06-06 AU AU62609/96A patent/AU708090B2/en not_active Expired
- 1996-06-06 AT AT96921369T patent/ATE290350T1/en not_active IP Right Cessation
- 1996-06-06 JP JP50179097A patent/JP3709487B2/en not_active Expired - Lifetime
- 1996-06-06 KR KR1019970708695A patent/KR19990022216A/en not_active Ceased
- 1996-06-06 NZ NZ311313A patent/NZ311313A/en unknown
- 1996-06-06 WO PCT/US1996/009482 patent/WO1996039101A1/en active Search and Examination
- 1996-06-06 CA CA002223892A patent/CA2223892A1/en not_active Abandoned
- 1996-06-06 EP EP96921369A patent/EP0967934B1/en not_active Expired - Lifetime
- 1996-06-06 DE DE69634454T patent/DE69634454T2/en not_active Expired - Lifetime
- 1996-06-06 ES ES96921369T patent/ES2238076T3/en not_active Expired - Lifetime
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