WO1997019337A1

WO1997019337A1 - Case for fixing and supporting biological sample

Info

Publication number: WO1997019337A1
Application number: PCT/JP1996/003301
Authority: WO
Inventors: Teiji Takezaki
Original assignee: Teiji Takezaki
Priority date: 1995-11-17
Filing date: 1996-11-11
Publication date: 1997-05-29
Also published as: AU7507296A

Abstract

A fixing/supporting case used for storing mannan in which are buried biological samples collected by doctors from the human body and transferred to a pathological inspection room for microscopic inspection. A support plate and a cover plate for constituting the fixing/support case are so disposed as to be capable of being opened and closed, a perforated plate made of a rigid sheet with many holes is fitted into a support hole bored at the center of the support plate, and the flange of the perforated plate is clamped between the support plate and the cover plate.

Description

Description Case for supporting the fixation of biological samples Technical field The present invention provides a clinician to transfer a biological sample collected from a human body to a pathological examination room or the like, where the specimen is prepared into a sliced sample (preparation) ready for microscopic examination. In such a case, the clinician establishes the biological sample so that it does not deteriorate due to self-thawing or decay. That is, the biological sample is fixed and supported biologically and transferred to a pathological laboratory, etc., or dehydration, degreasing, or clearing is performed. Background Art Conventionally, it is related to a case for inserting a biological sample necessary for processing and then embedding. Background Art Conventionally, when performing this kind of work, clinicians are obscure as shown in Figure 30 For the embedding cassette with a flat embedding cassette 42 with a lid 41 formed of a perforated plate, one with a different hole size in the perforated plate was selected depending on the size and characteristics of the biological sample. In an embedded cassette The biological sample (not shown) is accommodated, the lid 41 is closed, and the biological sample is directly placed in a capsule (not shown) containing a deterioration preventing agent for the biological sample and transferred to a pathological examination room or the like ( (See Japanese Patent Application No. 7-169003).

Then, remove the embedded cassette 42 containing the biological sample from the capsule, open the lid 41 of the embedded cassette 42, and insert the biological sample contained therein and the slip attached to it. After confirming the quantity and identity of both, the biological sample is returned to the embedding cassette 42 again, and placed in each container containing alcohol or xylene as it is. In each case, the biological sample is sequentially subjected to dehydration, degreasing, clearing, and embedding of paraffin or resin, etc. for each treatment:

Then, above the biological sample, the above-mentioned empty embedding case is left with a slight gap. A bottom surface of the cutout 42 is arranged, and an embedding agent made of molten paraffin or resin is poured into the bottom surface of the cutout, and cooled and solidified by cooling, and fixed in the embedding agent. The biological sample is embedded together with the support, together with the bottom of the embedded cassette 42:

The above-mentioned embedding cassette has a certain strength such as rigidity in order to take out a biological sample from the embedding cassette and fix the biological sample with paraffin based on the embedding cassette. Therefore, it is made of a hard synthetic resin or plastic, etc., and the wear of the cassette cannot be reduced below a certain limit. Therefore, if the embedded cassette becomes enormous, secure a storage place for it. , And movement becomes extremely difficult.

When information on biological samples is directly written on the cassette, it is written on the side of the cassette.However, the space is so small that it is hard to write, and it is difficult to read, so it is difficult to confirm the contents of the cassette. Or cause confusion with other biological samples

Also, when handling multiple biological samples, it is often necessary to indicate the order in which the samples were collected and the location of the organs and other parts from where they were collected for testing purposes. If it is still in place, it can be grasped by marking the cassette, etc., but it should be checked when the biological sample falls off or is removed for processing. _R becomes difficult

In addition, it is necessary to prepare various types of embedding cassettes with different hole sizes in the perforated plate according to the size of the biological sample, etc., resulting in high cost.

An object of the present invention is to provide a clinician with a biological sample contained therein, such as a conventional embedding cassette, which is moved to a laboratory or the like, as in a large hospital or a large laboratory. Even if the volume becomes enormous, it is necessary to ensure that there is no difficulty in securing a large space for storing the embedding cassettes and a space for dehydration, degreasing, and transparent treatment. It is to reduce the cost of stocking the embedded cassette:

Another purpose is to simplify the description of information on biological samples and prevent so-called contamination phenomena, such as making it difficult to confirm the contents of cassettes and confusing them with other biological samples. It is to stop.

Another purpose is to determine the order in which the samples were collected and from which parts, such as organs, even if the samples dropped out or were removed from the cassette when multiple biological samples were established. The purpose of this is to make it easy to grasp the positional relationship, etc., and to avoid confusion when processing samples later. DISCLOSURE OF THE INVENTION A fixing support case for a biological sample according to the present invention is a fixing support case for fixing a biological sample in a state in which the biological sample is embedded in a fixing support, wherein the support forming a part of the fixing support case is provided. A lid piece is provided on the piece so as to be openable and closable, and a perforated dish is fitted into a support hole formed in the center of one of the support piece and the lid piece. The overhanging flange is sandwiched between the support piece and the lid piece.

Further, the case for fixing and supporting a biological sample according to the present invention is a fixing support case for fixing a biological sample in a state where the biological sample is embedded in a fixing support agent, wherein the support piece constituting a part of the fixing and supporting case is provided. The support piece and the cover piece are formed by folding a hard sheet so that it can be opened and closed from the middle, and a support hole is formed in the center of the support piece. At the periphery, a flange protruding from the outer periphery of the opening of the perforated dish made of a hard liquid-permeable sheet is hooked so that it can be freely attached and detached. A predetermined quantity is accommodated in a desired arrangement state inside the bottom of a perforated dish formed of a sexual sheet, and fixed above and around the biological sample with a sample preserving and preservative stable solution such as glucomannan and formalin. Filling the support with the biological sample As well as fixing so as not to quality and by Uni support does not move within the porous dish liquid permeability.

In this way, the biological sample in the inferior state accommodated in the fixing support case is put as it is in another capsule containing a gelling agent mixed with a deterioration preventing agent of the biological sample together with the fixing support case. To a pathological laboratory. Then, the fixing support case accommodated in the capsule is taken out, and then put directly into each container containing alcohol or xylene, etc., and the biological sample is sequentially dehydrated, degreased, transparent, and removed from paraffin or resin or the like. Immersed in an embedding medium.

The biological sample in the fixing support case in this manner is immersed in alcohol as it is, dehydrated and degreased, immersed in xylol to remove alcohol (penetration), and further purified with paraffin or paraffin. Replace with the embedding agent by immersing it in an embedding agent made of resin, etc., and then take it out of the fixed support case in the form of a gel block embedded in the fixing support, and place it on the embedding dish as it is Place on:

An embedding support base such as an embedding cassette which is separately prepared is arranged above the gel-like block with a slight gap, and from above, a molten paraffin or resin or the like is placed from above. Injecting an embedding agent consisting of, followed by cooling to solidify by cooling and embedding a biological sample in the embedding agent together with the fixing support together with the bottom surface of the embedding support base It is.

Further, the case for supporting fixation of a biological sample according to the present invention is a fixing support case for fixing a biological sample in a state where the biological sample is embedded in a fixing support, wherein the flat case is formed by a perforated plate constituting the case for fixing support. A container and a cover plate are fitted openably and closably, and a window hole for installing a liquid-permeable perforated dish is formed in one of the flat container and the cover plate. A support frame protrudes inward, and a flange at the periphery of the opening of the perforated dish is sandwiched between the support frame and the inner surface of the lid plate or between the support frame and the bottom of the flat container. .

A biological sample collected by a clinician from a human body is stored in the bottom of a perforated dish, which is locked in a window formed in either the lid plate or the bottom of the flat container, and these are separately placed in a prone state. Put in a prepared capsule, soak in the alteration preventive agent in it, fix it so that the biological sample does not deteriorate in the embedding cassette, and hold it. Into a closed embedding cassette that is fitted to a flat container, and the embedding cassette is transferred to a predetermined treatment plant, where the cassettes are sequentially dehydrated, degreased, transparent, and embedded. Process, then place biological sample on embedding dish At the same time, the bottom of the flat container (embedding support base) of the empty embedding cassette is placed on top of it, and it is made of molten paraffin from above the biological sample via the embedding cassette. The embedding agent is injected, the biological sample is directly embedded in the embedding agent, and the embedding agent is cooled and solidified. At least a part of the bottom (flat container) of the embedding cassette (embedding) Brief description of the drawings Fig. 1 is a plan view of an embodiment of a biological sample anchoring support case according to the present invention. FIG. 2 is a sectional view taken along the line II-II in FIG.

FIG. 3 is a perspective view of a part of FIG. 1 and FIG.

FIG. 4 is a perspective view of another part of FIGS. 1 and 2

FIG. 5 is a perspective view of another embodiment of FIG.

FIG. 6 is a perspective view of still another embodiment of FIG.

FIG. 7 is a cross-sectional view of another embodiment of the biological sample fixing support case of the present invention. FIG. 8 is a plan view of a part of FIG.

FIG. 9 is a cross-sectional view taken along the line IX—IL of FIG.

FIG. 10 is a plan view of still another embodiment of the biological sample fixing support case of the present invention.

FIG. 11 is a plan view of still another embodiment of the biological sample fixing support case of the present invention.

FIG. 12 is a plan view of still another embodiment of the biological sample fixing support case of the present invention.

FIG. 13 is a cross-sectional view taken along the line XIII-XIII of FIG.

FIG. 14 is a perspective view of an embodiment of the cassette for embedding a biological sample of the present invention, which is different from FIGS. 1 to 13.

FIG. 15 is a cross-sectional view taken along line II-II of the embedding cassette of FIG. FIG. 16 is a perspective view of a part of FIG. FIG. 17 is a perspective view of another embodiment of the present invention.

FIG. 18 is a vertical cross-sectional view taken along the line VV of the embodiment of FIG. 17 in use. FIG. 19 is a perspective view of yet another embodiment of the present invention:

FIG. 20 is a vertical cross-sectional view of the VII-VII line portion showing the use state of FIG. 19.

Fig. 21 is a perspective view of an embodiment in which a part of the embodiment shown in Fig. 19 is modified: Fig. 22 is a sectional view taken along line IX-IX of the embodiment shown in Fig. 19 in use. FIG. 23 is a perspective view of an embodiment in which the embodiment of FIG. 19 is partially modified:

FIG. 24 is a cross-sectional view taken along line XI-XI of the embodiment of FIG. 2 in use: FIG. 25 is a perspective view of the embodiment of FIG. 23 with a partially modified embodiment. .

FIG. 26 is a cross-sectional view taken along the line XIII-XIII of the embodiment of FIG. 25. FIG. 27 is a perspective view of another part of FIG.

FIG. 28 is a perspective view of another embodiment of FIG.

FIG. ₂₉ is a perspective view of still another embodiment of FIG. 27.

FIG. 30 shows a conventional case for supporting and fixing a biological sample. ₍ Best Mode for Carrying Out the Invention) An embodiment of the present invention will be described with reference to FIGS. 1 to 3 of the accompanying drawings. The fixing support case for the sample is made of a rectangular liquid-permeable sheet that has immersion properties over the entire surface, such as cardboard or thick Japanese paper, or a hard synthetic resin such as polypropylene or polyethylene terephthalate, cardboard, or paraffin paper. It consists of a rectangular liquid-permeable sheet 1 with a number of small holes 1a perforated in a predetermined part of the device, etc. And a support hole 5 is formed in the center of the support piece 3

On the inner surface of the peripheral portion 6 of the support hole 5, a flange 9 projecting from the outer peripheral edge of the opening 8 of the perforated dish 7 formed of a liquid-permeable sheet made of a transparent hard synthetic resin is provided. , Hang it.

The clinician takes the required biological sample 12 from the human body and As shown in FIG. 2, a predetermined quantity is stored in the bottom part 13 of the perforated dish 7 formed of a liquid-permeable sheet in a predetermined state as shown in FIG. 2, and samples such as glucomannan and formalin are placed above and around the biological sample 12. Fill fixing support 14 consisting of preservative and preservative stabilizer:

Then, fold the lid piece 4 from the folding line 2 so as to overlap the support piece 3, close the opening 8 of the perforated dish 7, and fold the tongue piece 15 with a bending line so that the lid piece 4 does not open Fold it, insert it into the pores 16 formed in the support piece 3, roll it up in the protruded state in Fig. 2, and attach the locking pieces 17 protruding on both sides of the lid piece 4 to both sides of the support piece 3 if the concave portion 1 8 formed _on: to retain its state ₌

In this state, the biological sample is placed in a capsule (not shown) prepared in advance and immersed in a gelling agent for glucomannan (not shown) to gel the glucomannan. 1 and 2 are fixed so that they do not deteriorate, and are supported integrally with the perforated dish 7 so that they do not move together.

In this way, the biological sample 12 housed in the fixing support case is transferred to a pathological examination room or the like while being placed in the capsule, where it is fixed in the capsule. The support case is taken out and placed as it is in each container (not shown) containing alcohol or xylene, etc., and the biological sample 12 is sequentially dehydrated, degreased, passed through, and wrapped in paraffin or resin or the like as before. After being immersed in the mounting medium, the biological sample 12 is taken out of the fixing support case in the form of a gel-like block embedded in the fixing support 14, and the biological sample 12 is left as it is in an embedding dish (shown in the figure). No) Place on top.

Thereafter, as described in the specification of Japanese Patent Application No. 7-169003 related to the invention of the same inventor as the inventor of the present invention, another space is provided at the upper part of the gel-like block with a slight gap. The bottom surface of the embedding support base prepared in the above is placed, and an embedding agent made of molten paraffin or resin is injected from above, and it is cooled and solidified by cooling the embedding agent. The biological sample 12 is embedded in the mounting medium together with the fixing support 14 together with the bottom surface of the embedding support base.

Although the embodiment of the present invention has been described with reference to FIGS. 1 to 3 of the accompanying drawings, the present invention is not limited to the embodiment, and its configuration is partly within the scope of the present invention. It is possible to implement with partial changes or additions,

For example, as shown in FIG. 10, the shape of the support piece 3 and the lid piece 4 is changed to a shape having only the tongue piece 15 without the concave notch 18 and the locking piece 17 shown in FIG. The tongue piece 15 may be bent at the bending line 15a, inserted into the fine hole 16 formed in the support piece 3, and rolled up in the state shown in FIG.

Also, as shown in FIG. 11, the shape of the support piece 3 and the lid piece 4 is changed by simply folding the wire without providing the concave notch 18, the locking piece 17, and the tongue piece 15 shown in FIG. It may be a shape that only folds at 2. In this case, the supporting piece 3 and the lid piece 4 are folded, then the appropriate places that do not affect the perforated dish 7 are fastened with a stapler or a clip or a pin (not shown) and folded. It is not shown that the support piece 3 and the cover piece 4 are held flat by a stapler, a clip, a pin, or the like, as described above. It goes without saying that it can be applied in the example.

Further, instead of forming the perforated dish 7 with a transparent hard synthetic resin liquid-permeable sheet as in the embodiment shown in FIGS. 1 to 3, it is made of a translucent or opaque hard synthetic resin. The perforated dish 7 may be formed of a liquid-permeable sheet made of a transparent hard synthetic resin. As shown in FIGS. 7 to 9, a window hole 20 is formed in the center of the perforated dish 7a, and a liquid-permeable sheet-like flake 21 with a frame 19 is placed inside the window hole 20. The sheet-like flakes 21 are preferably made of a material to which the fixing support agent 14 does not easily adhere, such as a cloth made of synthetic resin fibers.

As shown in FIGS. 1 to 3 and FIGS. 7 to 9, a support hole 5 is formed in the center of the support piece 3, and a hard synthetic resin is formed on the inner surface of the peripheral portion 6 of the support hole 5. Instead of hooking the flange 9 projecting from the outer periphery of the opening 8 of the perforated dish 7 integrally formed of a resin-permeable sheet, in a hook-and-loop manner, as shown in Figs. 12 and 13 As shown, a perforated dish 7b may be formed integrally with the support piece 3 at the center thereof. In the reference numerals of FIGS. 7 to 13 showing another embodiment, the aforementioned FIGS. Parts having the same reference numerals as those used in the embodiments of the present invention have the same names and functions. It is like.

After the perforated dish 7, 7a, or 7b is filled with the fixing support 14, a hydrophilic sheet 10 having an affinity for the fixing support 14 is disposed in the opening 8. ( ₃₎ Since the hydrophilic sheet 10 adheres well to the fixing support 14, by arranging the hydrophilic sheet 10, the fixing support 14 can be fixed more reliably. In this case, it is possible to lower the concentration of the supporter: The hydrophilic sheet 10 is made of a material which is hydrophilic and easily adheres to the surface of the fixing supporter 14, and is made of alcohol. Use materials that have properties incompatible with ruxylene, such as Japanese paper, sponge slices, and cloth.

Further, as shown in FIG. 4, a reference marker 11 may be displayed at least on one side edge of the hydrophilic sheet 10 .The reference marker 11 may be used when a plurality of biological samples 12 are put. This is useful when it is necessary to indicate the order in which the biological samples 12 were collected, and the positional relationship such as from which part of the organ to be collected, etc.For example, multiple biological samples starting from the reference marker 11 It is decided in advance that 1 and 2 should be arranged according to a certain standard, so that confusion does not occur when embedding.

Further, instead of displaying the reference mark 11 on at least one side edge of the hydrophilic sheet 10 as in the embodiment of FIG. 4, one corner of the square hydrophilic sheet 10 is provided as shown in FIG. A notch 11a is formed on the surface of the hydrophilic sheet 10 as a reference mark, or a protrusion 11b is formed at one corner of the hydrophilic sheet 10 as shown in FIG. It is also possible.

In each embodiment of the present invention, a liquid-permeable sheet is double-folded from the middle folding line, and a support plate is provided with a liquid-perforated dish made of a synthetic resin sheet. Therefore, the thickness of the entire anchoring support case is smaller than that of the conventional one, and when a clinician moves a biological sample in the anchoring support case to an examination room, etc. Even if the number of biological samples becomes enormous, such as in a place, there is no difficulty in securing a vast place to store them. In addition, since the number of treatments per unit of time for dehydration, degreasing, and clearing increases, the overall treatment speed increases and the cost decreases. In addition, the supporting piece and the cover piece need only be made of an inexpensive material, such as cardboard or paraffin paper, as they only need to hold the perforated dish when transferring the biological sample and have hardness enough to withstand multiple bending. Can be. As a result, the cost can be significantly reduced as compared with the case where a conventional embedding cassette is used.

In addition, when a perforated dish is integrally formed at the center of the support piece, the cost can be further reduced because the time and labor required for separately manufacturing the perforated dish can be reduced ₍ :

Also, after folding the supporting piece and the lid piece, fix the appropriate places that do not affect the perforated dish with staples / tapes or clips or pins so that the folded state can be maintained. For example, the processing time can be shortened and the number of cases processed per unit time can be shortened compared with the case where the locking piece is fitted or the tongue piece is rolled into the pore. Due to the nature of the shape, there were few planes with no holes, so it was difficult to directly write the items related to the sample on the cassette.However, in the case of the present invention, the overall shape in the folded state was almost It is flat and has a wide flat area around the multi-hole plate, making it easy to fill out the case directly: This eliminates the need to create a separate slip and prevents slips from being misplaced or lost thing In addition, when the perforated dish is made of transparent synthetic resin, the contents can be checked without opening the case, and the sample can be checked. You can also easily fill out the case

Also, instead of integrally forming the perforated dish with a liquid-permeable sheet made of a hard synthetic resin, a window is formed in the center of the multi-hole dish, and a liquid-permeable sheet with a frame is provided inside the window. In the case of forming by forming a sheet-shaped flake, it is possible to use the perforated dish multiple times by replacing only this sheet-shaped flake.

Further, by disposing a hydrophilic sheet having affinity for the fixing support such as Japanese paper at the opening of the perforated dish filled with the fixing agent, the fixing support can be more securely fixed. However, dropping of biological samples can be prevented. Further, since the sheet increases the fixing strength, the concentration of the fixing support can be reduced, and the cost can be further reduced.

Also, when fixing multiple biological samples, a reference mark is displayed on the hydrophilic sheet. By arranging biological samples based on this, it is possible to easily understand the order in which the samples were collected and the positional relationship of the organs and other parts from which they were collected, and to confuse them when processing the samples thereafter. Does not occur.

An embodiment of the present invention will be described with reference to FIGS. 14 to 16 of the accompanying drawings. A flat plate 22 formed of the same porous plate as a flat container 22 formed of a porous plate of a hard synthetic resin or the like is provided with a lid plate 23 formed of the same. In the embedding cassette which is openably and closably fitted with the engaging piece 24 and the locking piece 24a on the side and the engaging hole 25 and the locking portion 25a on the flat container 1, etc. A window hole 27 is formed in the container 1 for hemorrhoids of the liquid-permeable perforated dish 26, and a support frame 28 is protruded from the periphery of the window hole 27 toward the inside of the flat container 22. And a flange 30 on the periphery of the opening 29 of the perforated dish 26 is sandwiched between the tip edge 28 a of the support frame 28 and the inner surface of the lid plate 23. The perforated dish 26 is integrally formed of a transparent hard synthetic resin liquid-permeable sheet

The biological sample 31 collected from the human body for examination or treatment by the clinician is placed in the bottom of the perforated dish 26 as shown in FIG. In such a state, they are placed in a separately prepared capsule and immersed in a denaturation inhibitor to fix the biological sample so that it does not deteriorate in the embedding cassette. Depending on the type of biological sample, transfer and Some of them fall off during processing.In such a case, for example, the fixing method using a fixing support disclosed in Japanese Patent Application No. 7-169003 ensures that the inside of the perforated dish 26 is securely inserted. That is, a fixing support 32 composed of a sample storage and preservative stabilizer such as glucomannan and formalin is filled above and around the biological sample 31, and these are preliminarily prepared in this state. The biological sample 31 is moved in an embedding cassette by putting in a separately prepared capsule (not shown) and immersing in a glucomannan gelling agent to gel the glucomannan. Not with it Integrally supported-After the above processing, the lid plate 23 is fitted into the flat container 22 to form a sealed embedded cassette, and the embedded cassette in that state is placed in a predetermined treatment plant. After transport, the cassettes are sequentially treated with dehydrating, degreasing, clearing, and embedding agents, and then the cover plate 23 of the embedding cassette is opened and embedded in the perforated dish 26 into the fixing support 32. Remove the biological sample 31 in a state where it has been placed. Place it on an embedding dish (not shown) and The bottom of the embedding support base is placed on the upper part, and an embedding agent made of molten paraffin is injected from above the biological sample 31 embedded in the fixing support 32 via the flat container 22. Then, the biological sample 31 is directly embedded in the embedding medium, the embedding medium is cooled and solidified, and the biological sample 31 embedded in the fixing support 32 is sliced with a microtome.

(Not shown). The embedding support base used for embedding may be prepared separately, or a flat container 1 of an embedding cassette containing four or more biological samples may be used.

The embedding cassette of the present invention is not limited to the above-described embodiment, and may be partially modified or partially added within the scope of the present invention: For example, in the above-described embodiment, a window hole 27 is provided at the center of the flat container 22, and a support frame 28 is protruded from the inner peripheral edge of the window hole 27 toward the inside of the flat container 22. The force holding the flange 30 of the perforated dish 26 between the tip edge 28 of the support frame 28 and the inner surface of the lid plate 23, as shown in FIGS. 17 and 18, A window hole 27 is provided in the cover plate 23, and a support frame 28 is protruded from the inner peripheral edge of the window hole 27, and a front edge 28a of the support frame 28 and the inner surface of the flat container 22 are formed. It is also possible to clamp the flange 30 of the perforated dish 26 with:

In the above embodiment, the perforated dish 26 is formed of a transparent hard synthetic resin liquid-permeable sheet, but may be translucent or opaque:

If the opening area of the window hole 27 is made larger than the size of the bottom surface of the embedding cassette, a grid 38 may be formed in the window hole 27. This strengthens the bottom of the perforated dish 26 and increases its strength when used as an embedded support base. Also, as compared with the case where only the window 27 is provided, the portion that comes into contact with the embedding agent is increased, so that the embedding can be performed more integrally.

Although the lid plate 23 of the above embodiment is described as being made of a hard synthetic resin, a stainless steel or other stainless steel is used as shown in FIGS. 19 and 20 in place of the synthetic resin. In this case, the fitting of the engaging piece 34 to the engaging hole 35 is the same as that of the above-described embodiment, but the other of the engaging pieces is connected to the spring-like engaging element. 36, which engages with the engaging projections 37 provided on the flat container 22: Further, the embodiment shown in FIGS. 21 and 22 is the same as the embodiment shown in FIG. The difference is that the edge 36a of the child 36 is facing outward, whereas the edge 36b is facing inward.

Further, in the embodiment shown in FIGS. 23 and 24, the support frame 28 in each of the above-described embodiments protrudes along the inner peripheral edge of the window hole 27, while the outside of the cover plate 23 is provided. They differ in that they protrude along the periphery. This embodiment is different from the embodiments of FIGS. 19 to 22 in that the engagement element 36 is also used as the support frame 28, and the volume of the portion surrounded by the support frame 28 is increased. Therefore, a relatively large biological sample 31 can be accommodated.

Further, the embodiment shown in FIGS. 25 and 26 is different from the embodiment shown in FIGS. 23 and 24 in that the joint 36 is constituted by two parts, but is constituted integrally. Different c

FIGS. 18, 20, 22, 24, and 26 described above show a state in which the opening 29 of the perforated dish 26 faces downward. When accommodating 31 or holding the flange 30 of the perforated dish 26 between the support frame 28 and the inner surface of the flat container 22, the flat container 22 and the It is performed with the prone and upside down prone shown in Fig. 8,

In addition to the above-mentioned embodiment, after the perforated dish 26 is filled with the fixing support 32, the opening 29 of the perforated dish is filled with a hydrophilic sheet 33 having an affinity for the fixing support 32. May be arranged. Since the hydrophilic sheet 33 adheres well to the fixing support 32, by arranging the hydrophilic sheet 33, the fixing support 32 can be fixed more reliably. It is possible to lower the concentration. The hydrophilic sheet 33 is made of a material which is hydrophilic and easily adheres to the surface of the fixing support 32 and has a property of being insoluble in alcohol and xylene, for example, a thin section of Japanese paper or sponge. Use a cloth or the like.

Further, as shown in Fig. 27, a reference mark 39 may be displayed at least on one side edge of the hydrophilic sheet 33. The reference mark 39 may include a plurality of biological samples 31 in particular. This is useful when it is necessary to indicate the order in which the biological samples 31 were collected, and from which part of the organ to be collected, etc. It is determined in advance that a plurality of biological samples 31 are to be arranged on a certain basis as a starting point, so that confusion does not occur when embedding.

Furthermore, instead of displaying the reference mark 39 on at least one side edge of the hydrophilic sheet 33 as in the embodiment of FIG. 27, a square hydrophilic sheet 33 as shown in FIG. A notch 33a is formed in one corner of the hydrophilic sheet 33 as a reference mark, or a protrusion 33b is formed in one corner of the hydrophilic sheet 33 as shown in FIG. It is also possible that

The embodiment of the present invention shown in FIGS. 14 and 2 is as shown in the upper trace. A window hole is formed in either the flat container or the cover plate of the embedding cassette, and the periphery of the window hole is formed. A support frame is protruded toward the inside of the embedding cassette, and a perforated dish containing a biological sample is placed and held between the flat container and the cover plate. It can accommodate biological samples of various sizes and characteristics without changing the size of the holes in the perforated plate, and the role of the basket during dewatering, degreasing, clearing, and embedding work from preventing the deterioration of biological samples And a biological specimen embedding cassette that can serve as an embedding support base when slicing by a microtome can be provided, and costs can be reduced.

In addition, since the perforated dish containing the biological sample is held between the flat container and the cover plate, the biological sample does not move or fall off, which facilitates handling such as transportation. If the sample is fixed, it can be more reliably prevented from falling off

Furthermore, by disposing a hydrophilic sheet having an affinity for the fixing support such as Japanese paper at the opening of the perforated dish filled with the fixing support, the fixing support can be more reliably fixed. In addition, the sheet increases the fixing strength, and the concentration of the fixing supporting agent can be reduced, and the cost can be further reduced. is there

When a plurality of biological samples are established, a reference sign is displayed on the hydrophilic sheet, and if the biological samples are arranged based on this, the order in which the samples were collected and from which part of the organs etc. the sample was collected The positional relationship, etc. can be easily grasped, and confusion does not occur when the sample is subsequently processed. Furthermore, when the perforated dish is made of a transparent synthetic resin, the contents can be seen through without opening the case, making it easy to confirm the sample. Therefore, during the embedding process, the type, number and state of the biological sample in the embedding cassette can be confirmed without opening and closing the lid plate.

Further, when unsold steel is used for the cover plate, the cover plate can be used a plurality of times, so that the cost can be further reduced. INDUSTRIAL APPLICABILITY As described above, this invention uses a porous dish, which is a main part of a fixing support case for fixing a biological sample embedded in a fixing support agent in a prone state, as described above. Since the sheet is formed using a sheet, the perforated dish can be formed simply by pressing at a low cost. It is particularly suitable and facilitates the pathological examination of a large number of patients and can be used to improve the accuracy of the examination.

Claims

The scope of the claims

1. In a fixing support case for fixing a biological sample embedded in a fixing support, a cover piece is provided on a support piece constituting a part of the fixing case so as to be openable and closable, and the support piece and the cover are provided. A perforated plate made of a hard sheet is fitted into a support hole formed in the center of one of the pieces, and a flange protruding outward around the opening edge of the perforated dish is formed with the support piece and the lid. Fixation of biological sample characterized by being sandwiched between pieces by a support case

2. In a fixing support case for fixing a biological sample in a state in which the biological sample is embedded in the fixing support, a cover piece is provided on a support piece that constitutes a part of the fixing support case, and the cover piece is automatically opened and closed. And a lid piece formed by folding a hard sheet openably and closably from the middle thereof, forming a support hole in the center of the support piece, and forming a hard liquid-permeable material on the inner side surface of the periphery of the support hole. A case for fixing and supporting a biological sample, wherein a flange protruding from an outer peripheral edge of an opening portion of a perforated dish formed of a sheet is hooked so as to be freely detachable.

3. A fixing support case for fixing a biological sample embedded in a fixing support in a prone state, which is provided on a support piece constituting a part of the fixing support case so as to be openable and closable, and the support piece and a lid are provided. A piece is formed by folding a hard sheet openably and closably from the middle thereof, a support hole is formed in the center of the support piece, and a hard liquid permeability is formed on the inner side surface of the periphery of the support hole. A flange protruding from the outer periphery of the opening of the perforated dish formed by the sheet is hooked so as to be freely detachable, and the opening of the perforated dish has an affinity for the fixing support agent. A case for fixing and supporting a biological sample, comprising a hydrophilic sheet.

4. The case for fixing and supporting a biological sample according to claim 2 or 3, wherein the hard sheet forming the supporting piece and the lid piece is made of a hard synthetic resin.

5. The case for fixing and supporting a biological sample according to claim 2 or 3, wherein the hard sheet forming the support piece and the lid piece is made of hard cardboard.

6. The case for fixing and supporting a biological sample according to claim 2, wherein the hard sheet forming the support piece and the lid piece is made of hard paraffin paper.

7. The perforated dish having an opening and a bottom is formed of a liquid-permeable sheet made of a hard synthetic resin, and a flange is integrally formed on an outer peripheral edge of the opening of the perforated dish. Is the case for supporting the fixation of the biological sample described in 3.

8. The perforated dish having an opening and a bottom has a window at the bottom thereof, and a liquid-permeable sheet-like thin piece is detachably provided inside the window. A case for supporting fixation of the biological sample described.

9. The biological sample φ fixing support case according to claim 3, wherein the sheet provided at the opening of the perforated dish is made of Japanese paper.

10. The case for fixing and supporting a biological sample according to claim 3, wherein the sheet provided at the opening of the perforated dish is a sponge.

11. The case for fixing and supporting a biological sample according to claim 3, wherein the sheet provided in the opening of the perforated dish is a cloth.

12. The case for fixing and supporting a biological sample according to claim 3, wherein the reference mark is displayed near at least one side edge of the sheet provided in the opening of the perforated dish.

1 3. In a fixing support case for fixing a biological sample in a state where the biological sample is embedded in the fixing support, a hard sheet is folded openably and closable from the middle, and the support piece and the liquid-permeable lid piece Wherein a perforated dish is formed integrally with the support piece at the center thereof, and the opening of the perforated dish is directed toward the lid.

14. In a fixing support case for fixing a biological sample embedded in a fixing support, a hard sheet is folded openably and closable from the middle of the support sheet and a liquid-permeable lid piece. Forming a perforated dish integrally with the support piece at the center of the support piece, and turning the opening of the perforated dish toward the side of the lid, and at the opening of the perforated dish, A case for supporting and fixing a biological sample, comprising a hydrophilic sheet having high affinity.

15. In a fixing support case for fixing a biological sample in a state where the biological sample is embedded in the fixing support, a flat container formed of a perforated plate constituting the fixing support case and a lid plate are fitted openably and closably. And a perforated dish permeable to one of the flat container and the lid plate. Forming a window hole for installing the same, a support frame protruding from the periphery of the window hole toward the inside of the flat container, and between the support frame and the inner surface of the lid plate or between the support frame and the flat container. A biological sample embedding cassette characterized by sandwiching a flange around the opening of the perforated dish with a bottom of the biological sample.

. 1 6 containers and embedding cassettes of a biological sample according to claim 1 5, wherein that you have grating is provided in the window hole formed on either one of the cover _plate;:

17. The biological sample embedding cassette according to _claim 15 or 16, _{wherein the} flat container is formed of a hard synthetic resin and the cover plate is formed of _{stainless steel.}

1 8. A spring that engages with an engagement piece that fits into an engagement hole of a flat container at one edge of a lid plate made of stainless steel, and that engages with one side of the lid plate at the other edge. 18. The biological sample embedding cassette according to claim 17, wherein the engagement member is projected.

19. The biological sample embedding cassette according to any one of claims 15 to 18, wherein a hydrophilic sheet is provided at an opening of the perforated dish.