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WO1999005266A3 - Transfert de noyau entre des especes differentes - Google Patents

Transfert de noyau entre des especes differentes Download PDF

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Publication number
WO1999005266A3
WO1999005266A3 PCT/US1998/015387 US9815387W WO9905266A3 WO 1999005266 A3 WO1999005266 A3 WO 1999005266A3 US 9815387 W US9815387 W US 9815387W WO 9905266 A3 WO9905266 A3 WO 9905266A3
Authority
WO
WIPO (PCT)
Prior art keywords
nuclear transfer
trans
donor cells
species nuclear
oocytes
Prior art date
Application number
PCT/US1998/015387
Other languages
English (en)
Other versions
WO1999005266B1 (fr
WO1999005266A2 (fr
Inventor
Neal L First
Tanja Dominko
Maissam Mitalipoua
Original Assignee
Wisconsin Alumni Res Found
Neal L First
Tanja Dominko
Maissam Mitalipoua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found, Neal L First, Tanja Dominko, Maissam Mitalipoua filed Critical Wisconsin Alumni Res Found
Priority to AU85875/98A priority Critical patent/AU8587598A/en
Publication of WO1999005266A2 publication Critical patent/WO1999005266A2/fr
Publication of WO1999005266A3 publication Critical patent/WO1999005266A3/fr
Publication of WO1999005266B1 publication Critical patent/WO1999005266B1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

On décrit un procédé permettant de produire des embryons clonés par transfert nucléaire à partir de cellules donneuses différenciées. Le procédé consiste à cultiver des cellules donneuses différenciées dans un milieu pauvre en sérum et à utiliser en tant qu'ovocyte récepteur des ovocytes de bovins pour des cellules donneuses provenant d'espèces différentes. On peut utiliser ce procédé pour produire des animaux génétiquement identiques et des animaux transgéniques.
PCT/US1998/015387 1997-07-26 1998-07-24 Transfert de noyau entre des especes differentes WO1999005266A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85875/98A AU8587598A (en) 1997-07-26 1998-07-24 Trans-species nuclear transfer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5310397P 1997-07-26 1997-07-26
US60/053,103 1997-07-26

Publications (3)

Publication Number Publication Date
WO1999005266A2 WO1999005266A2 (fr) 1999-02-04
WO1999005266A3 true WO1999005266A3 (fr) 1999-04-15
WO1999005266B1 WO1999005266B1 (fr) 1999-09-23

Family

ID=21981947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/015387 WO1999005266A2 (fr) 1997-07-26 1998-07-24 Transfert de noyau entre des especes differentes

Country Status (2)

Country Link
AU (1) AU8587598A (fr)
WO (1) WO1999005266A2 (fr)

Families Citing this family (60)

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JP2002505100A (ja) * 1998-03-02 2002-02-19 ユニヴァーシティー オヴ マサチューセッツ アパブリック インスティテューション オヴ ハイアー エデュケイション オヴ ザ コモンウェルス オヴ マサチューセッツ リプレゼンティド バイイッツ アム 種間核移植によって作製された胚性または幹細胞様細胞株
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CN1362965A (zh) 1999-06-30 2002-08-07 先进细胞技术公司 细胞质转移使受体细胞去分化
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AU7701900A (en) * 1999-09-14 2001-04-17 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
BR0016561A (pt) * 1999-12-20 2002-09-10 Univ Massachusetts Public Inst Células embrionárias ou similares a células tronco produzidas por transplantes nuclear em espécie cruzadas
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Also Published As

Publication number Publication date
WO1999005266B1 (fr) 1999-09-23
AU8587598A (en) 1999-02-16
WO1999005266A2 (fr) 1999-02-04

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