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WO1999005266B1 - Transfert de noyau entre des especes differentes - Google Patents

Transfert de noyau entre des especes differentes

Info

Publication number
WO1999005266B1
WO1999005266B1 PCT/US1998/015387 US9815387W WO9905266B1 WO 1999005266 B1 WO1999005266 B1 WO 1999005266B1 US 9815387 W US9815387 W US 9815387W WO 9905266 B1 WO9905266 B1 WO 9905266B1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
nuclear transfer
donor
hours
enucleated
Prior art date
Application number
PCT/US1998/015387
Other languages
English (en)
Other versions
WO1999005266A3 (fr
WO1999005266A2 (fr
Inventor
Neal L First
Tanja Dominko
Maissam Mitalipoua
Original Assignee
Wisconsin Alumni Res Found
Neal L First
Tanja Dominko
Maissam Mitalipoua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found, Neal L First, Tanja Dominko, Maissam Mitalipoua filed Critical Wisconsin Alumni Res Found
Priority to AU85875/98A priority Critical patent/AU8587598A/en
Publication of WO1999005266A2 publication Critical patent/WO1999005266A2/fr
Publication of WO1999005266A3 publication Critical patent/WO1999005266A3/fr
Publication of WO1999005266B1 publication Critical patent/WO1999005266B1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

On décrit un procédé permettant de produire des embryons clonés par transfert nucléaire à partir de cellules donneuses différenciées. Le procédé consiste à cultiver des cellules donneuses différenciées dans un milieu pauvre en sérum et à utiliser en tant qu'ovocyte récepteur des ovocytes de bovins pour des cellules donneuses provenant d'espèces différentes. On peut utiliser ce procédé pour produire des animaux génétiquement identiques et des animaux transgéniques.
PCT/US1998/015387 1997-07-26 1998-07-24 Transfert de noyau entre des especes differentes WO1999005266A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85875/98A AU8587598A (en) 1997-07-26 1998-07-24 Trans-species nuclear transfer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5310397P 1997-07-26 1997-07-26
US60/053,103 1997-07-26

Publications (3)

Publication Number Publication Date
WO1999005266A2 WO1999005266A2 (fr) 1999-02-04
WO1999005266A3 WO1999005266A3 (fr) 1999-04-15
WO1999005266B1 true WO1999005266B1 (fr) 1999-09-23

Family

ID=21981947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/015387 WO1999005266A2 (fr) 1997-07-26 1998-07-24 Transfert de noyau entre des especes differentes

Country Status (2)

Country Link
AU (1) AU8587598A (fr)
WO (1) WO1999005266A2 (fr)

Families Citing this family (60)

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US7696404B2 (en) 1996-08-19 2010-04-13 Advanced Cell Technology, Inc. Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
CA2262817A1 (fr) * 1996-08-19 1998-02-26 James Robl Lignees de cellules embryonnaires ou de type souche produites par transplantation nucleaire croisee d'especes
JP2002505100A (ja) * 1998-03-02 2002-02-19 ユニヴァーシティー オヴ マサチューセッツ アパブリック インスティテューション オヴ ハイアー エデュケイション オヴ ザ コモンウェルス オヴ マサチューセッツ リプレゼンティド バイイッツ アム 種間核移植によって作製された胚性または幹細胞様細胞株
EP1067834A4 (fr) * 1998-03-16 2002-01-23 Garelag Pty Ltd Transfert de noyau de cellules porcines
CA2334953A1 (fr) * 1999-06-30 2001-01-04 Woo-Suk Hwang Procede d'obtention d'embryons humains clones au moyen de techniques de transplantation inter-especes de noyaux
CN1362965A (zh) 1999-06-30 2002-08-07 先进细胞技术公司 细胞质转移使受体细胞去分化
EP1109891A4 (fr) * 1999-06-30 2004-11-17 Hwang Woo Suk Procede de production de vaches clonees
WO2007019398A1 (fr) 2005-08-03 2007-02-15 Advanced Cell Technology, Inc. Procedes ameliores de reprogrammation de cellules somatiques animales
US7621606B2 (en) 2001-08-27 2009-11-24 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
NZ508739A (en) * 1999-06-30 2002-11-26 Woo Suk Hwang Method for producing cloned tigers by employing inter-species nuclear transplantation technique
AU7701900A (en) * 1999-09-14 2001-04-17 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
BR0016561A (pt) * 1999-12-20 2002-09-10 Univ Massachusetts Public Inst Células embrionárias ou similares a células tronco produzidas por transplantes nuclear em espécie cruzadas
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JP2019509059A (ja) 2016-02-16 2019-04-04 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 変異型キヌレニナーゼ遺伝子を有する非ヒト動物
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JP7026678B2 (ja) 2016-09-30 2022-02-28 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｃ９ｏｒｆ７２座位中にヘキサヌクレオチドリピート伸長を有する非ヒト動物
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US11696572B2 (en) 2017-06-27 2023-07-11 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ASGR1 locus
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BR112020001364A2 (pt) 2017-07-31 2020-08-11 Regeneron Pharmaceuticals, Inc. métodos para testar e modificar a capacidade de uma crispr/cas nuclease.
EP3585160A2 (fr) 2017-07-31 2020-01-01 Regeneron Pharmaceuticals, Inc. Animaux non humains reporteurs pour crispr et leurs utilisations
MX394999B (es) 2017-09-29 2025-03-24 Regeneron Pharma Animales no humanos que comprenden un locus ttr humanizado y metodos de uso
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CN117178959A (zh) 2019-04-04 2023-12-08 瑞泽恩制药公司 包括人源化凝血因子12基因座的非人动物
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CN114008193A (zh) 2019-06-27 2022-02-01 瑞泽恩制药公司 Tdp-43蛋白病的建模
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EP4426107A1 (fr) 2021-11-04 2024-09-11 Regeneron Pharmaceuticals, Inc. Animaux non humains comprenant un locus cacng1 modifié
CN118632622A (zh) 2021-12-08 2024-09-10 瑞泽恩制药公司 突变型肌纤蛋白疾病模型及其用途
WO2023122506A1 (fr) 2021-12-20 2023-06-29 Regeneron Pharmaceuticals, Inc. Animaux non humains comprenant des loci ace2 et tmprss humanisés
CN113943712B (zh) * 2021-12-20 2022-03-22 南京岚轩生物科技有限公司 一种电融合缓冲液、其制备方法及电融合方法
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GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
CA2262817A1 (fr) * 1996-08-19 1998-02-26 James Robl Lignees de cellules embryonnaires ou de type souche produites par transplantation nucleaire croisee d'especes
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells

Also Published As

Publication number Publication date
WO1999005266A3 (fr) 1999-04-15
AU8587598A (en) 1999-02-16
WO1999005266A2 (fr) 1999-02-04

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