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WO1999036571A3 - Method for the detection or nucleic acid of nucleic acid sequences - Google Patents

Method for the detection or nucleic acid of nucleic acid sequences Download PDF

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Publication number
WO1999036571A3
WO1999036571A3 PCT/EP1999/000161 EP9900161W WO9936571A3 WO 1999036571 A3 WO1999036571 A3 WO 1999036571A3 EP 9900161 W EP9900161 W EP 9900161W WO 9936571 A3 WO9936571 A3 WO 9936571A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
detection
solid support
ligation
molecules
Prior art date
Application number
PCT/EP1999/000161
Other languages
French (fr)
Other versions
WO1999036571A2 (en
Inventor
Holger Klapproth
Hubert S Bernauer
Original Assignee
Biochip Technologies Gmbh
Holger Klapproth
Hubert S Bernauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biochip Technologies Gmbh, Holger Klapproth, Hubert S Bernauer filed Critical Biochip Technologies Gmbh
Priority to CA002318371A priority Critical patent/CA2318371A1/en
Priority to EP99907365A priority patent/EP1047794A2/en
Priority to JP2000540272A priority patent/JP2002517981A/en
Publication of WO1999036571A2 publication Critical patent/WO1999036571A2/en
Publication of WO1999036571A3 publication Critical patent/WO1999036571A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a novel method for the detection of a nucleic acid sequence within a nucleic acid molecule. The method of the invention relies on the combination of nucleic acid protection, ligation of oligonucleotides to the protected nucleic acid molecules and amplification of the ligation products. The detection of the amplified products is advantageously effected by converting the same to the single-stranded form and hybridizing the single-stranded form thereof to an array of single-stranded nucleic acid molecules of at least partially predetermined sequence fixed to a solid support. The solid support is preferably a chip. Detection of hybridized molecules can be effected according to conventional methods. The present invention additionally relates to a kit for carrying out the method of the invention.
PCT/EP1999/000161 1998-01-13 1999-01-13 Method for the detection or nucleic acid of nucleic acid sequences WO1999036571A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002318371A CA2318371A1 (en) 1998-01-13 1999-01-13 Method for the detection of nucleic acid sequences
EP99907365A EP1047794A2 (en) 1998-01-13 1999-01-13 Method for the detection or nucleic acid of nucleic acid sequences
JP2000540272A JP2002517981A (en) 1998-01-13 1999-01-13 Methods for detecting nucleic acid sequences

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98100485 1998-01-13
EP98100485.6 1998-01-13

Publications (2)

Publication Number Publication Date
WO1999036571A2 WO1999036571A2 (en) 1999-07-22
WO1999036571A3 true WO1999036571A3 (en) 1999-10-21

Family

ID=8231257

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000161 WO1999036571A2 (en) 1998-01-13 1999-01-13 Method for the detection or nucleic acid of nucleic acid sequences

Country Status (4)

Country Link
EP (1) EP1047794A2 (en)
JP (1) JP2002517981A (en)
CA (1) CA2318371A1 (en)
WO (1) WO1999036571A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8901287B2 (en) 2004-10-20 2014-12-02 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9410146B2 (en) 2009-09-14 2016-08-09 Qiagen Gaithersburg Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399589B2 (en) 1998-02-06 2008-07-15 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US6686151B1 (en) 1998-02-06 2004-02-03 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
ATE316152T1 (en) 1998-10-27 2006-02-15 Affymetrix Inc COMPLEXITY MANAGEMENT AND ANALYSIS OF GENOMIC DNA
CA2377739A1 (en) 1999-07-02 2001-01-11 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate, where the polymers have water-soluble or water-dispersible segments and probes bonded thereto
EP1111069A1 (en) * 1999-12-22 2001-06-27 BioChip Technologies GmbH Modified nucleic acids and their use
DK1132739T3 (en) 2000-05-16 2002-01-28 Biochip Technologies Gmbh Linker system for activating surfaces for bioconjugation and methods for using them
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
JP2002176985A (en) * 2000-12-14 2002-06-25 Hitachi Software Eng Co Ltd Method for detecting base sequence amplified by pcr and kit therefor
WO2002056021A2 (en) 2001-01-10 2002-07-18 Symyx Technologies Inc Polymer brushes for immobilizing molecules to a surface
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
US8288520B2 (en) 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
CN102414327B (en) 2009-05-01 2015-04-08 奇亚根盖瑟斯堡股份有限公司 A non-target amplification method for detection of RNA splice-forms in a sample
ES2615728T3 (en) 2010-01-29 2017-06-08 Qiagen Gaithersburg, Inc. Methods and compositions for purification and specific multiple nucleic acid sequence analysis
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
EP2678442B1 (en) 2011-02-24 2019-01-16 QIAGEN Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
US9315807B1 (en) 2013-01-26 2016-04-19 New England Biolabs, Inc. Genome selection and conversion method
US10087481B2 (en) 2013-03-19 2018-10-02 New England Biolabs, Inc. Enrichment of target sequences
EP3312295A1 (en) * 2013-03-19 2018-04-25 Directed Genomics, LLC Enrichment of target sequences
CN111979303A (en) * 2020-08-11 2020-11-24 上海奕谱生物科技有限公司 Nucleic acid detection kit, method and application thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012695A1 (en) * 1988-06-17 1989-12-28 Genelabs Incorporated Dna amplification and subtraction techniques
EP0372524A2 (en) * 1988-12-07 1990-06-13 The General Hospital Corporation Method of enrichment and cloning for DNA containing an insertion or corresponding to a deletion
WO1990009457A2 (en) * 1989-02-14 1990-08-23 Biogen, Inc. Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them
WO1995015974A1 (en) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Protecting nucleic acids and methods of analysis
WO1996036731A2 (en) * 1995-05-19 1996-11-21 Trustees Of Boston University Nucleic acid detection methods
WO1997019192A1 (en) * 1995-11-20 1997-05-29 Trustees Of Boston University Method and probe for detecting a target nucleic acid sequence
WO1997047640A1 (en) * 1996-06-14 1997-12-18 Sarnoff Corporation Nuclease protection assays

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012695A1 (en) * 1988-06-17 1989-12-28 Genelabs Incorporated Dna amplification and subtraction techniques
EP0372524A2 (en) * 1988-12-07 1990-06-13 The General Hospital Corporation Method of enrichment and cloning for DNA containing an insertion or corresponding to a deletion
WO1990009457A2 (en) * 1989-02-14 1990-08-23 Biogen, Inc. Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them
WO1995015974A1 (en) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Protecting nucleic acids and methods of analysis
WO1996036731A2 (en) * 1995-05-19 1996-11-21 Trustees Of Boston University Nucleic acid detection methods
WO1997019192A1 (en) * 1995-11-20 1997-05-29 Trustees Of Boston University Method and probe for detecting a target nucleic acid sequence
WO1997047640A1 (en) * 1996-06-14 1997-12-18 Sarnoff Corporation Nuclease protection assays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG M ET AL: "CLONING DIFFERENTIALLY EXPRESSED GENES BY LINKER CAPTURE SUBTRACTION", ANALYTICAL BIOCHEMISTRY, vol. 237, no. 1, 15 May 1996 (1996-05-15), pages 109 - 114, XP000587696 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8901287B2 (en) 2004-10-20 2014-12-02 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9410146B2 (en) 2009-09-14 2016-08-09 Qiagen Gaithersburg Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes

Also Published As

Publication number Publication date
EP1047794A2 (en) 2000-11-02
CA2318371A1 (en) 1999-07-22
WO1999036571A2 (en) 1999-07-22
JP2002517981A (en) 2002-06-25

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