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WO1999036571A3 - Procede de detection de sequences nucleotidiques - Google Patents

Procede de detection de sequences nucleotidiques Download PDF

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Publication number
WO1999036571A3
WO1999036571A3 PCT/EP1999/000161 EP9900161W WO9936571A3 WO 1999036571 A3 WO1999036571 A3 WO 1999036571A3 EP 9900161 W EP9900161 W EP 9900161W WO 9936571 A3 WO9936571 A3 WO 9936571A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
detection
solid support
ligation
molecules
Prior art date
Application number
PCT/EP1999/000161
Other languages
English (en)
Other versions
WO1999036571A2 (fr
Inventor
Holger Klapproth
Hubert S Bernauer
Original Assignee
Biochip Technologies Gmbh
Holger Klapproth
Hubert S Bernauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biochip Technologies Gmbh, Holger Klapproth, Hubert S Bernauer filed Critical Biochip Technologies Gmbh
Priority to JP2000540272A priority Critical patent/JP2002517981A/ja
Priority to EP99907365A priority patent/EP1047794A2/fr
Priority to CA002318371A priority patent/CA2318371A1/fr
Publication of WO1999036571A2 publication Critical patent/WO1999036571A2/fr
Publication of WO1999036571A3 publication Critical patent/WO1999036571A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un nouveau procédé de détection d'un séquence nucléotidique au sein d'une molécule d'acide nucléique. Le procédé de l'invention consiste principalement à combiner la protection de l'acide nucléique, la ligature d'oligonucléotides auxdites molécules d'acide nucléique protégées et l'amplification des produits de la ligature. La détection des produits amplifiés s'effectue avantageusement par la conversion de ces derniers, de sorte qu'ils se présentent sous une forme à simple brin, et par l'hybridation de leur forme à simple brin en un réseau de molécules d'acide nucléique simple brin d'au moins une séquence partiellement déterminée, fixée à un support solide. Le support solide est de préférence une pastille. La détection des molécules hybridées peut s'effectuer selon des procédés classiques. L'invention concerne également un kit pour la mise en oeuvre du procédé de l'invention.
PCT/EP1999/000161 1998-01-13 1999-01-13 Procede de detection de sequences nucleotidiques WO1999036571A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2000540272A JP2002517981A (ja) 1998-01-13 1999-01-13 核酸配列を検出するための方法
EP99907365A EP1047794A2 (fr) 1998-01-13 1999-01-13 Procede de detection de sequences nucleotidiques
CA002318371A CA2318371A1 (fr) 1998-01-13 1999-01-13 Procede de detection de sequences nucleotidiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98100485.6 1998-01-13
EP98100485 1998-01-13

Publications (2)

Publication Number Publication Date
WO1999036571A2 WO1999036571A2 (fr) 1999-07-22
WO1999036571A3 true WO1999036571A3 (fr) 1999-10-21

Family

ID=8231257

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000161 WO1999036571A2 (fr) 1998-01-13 1999-01-13 Procede de detection de sequences nucleotidiques

Country Status (4)

Country Link
EP (1) EP1047794A2 (fr)
JP (1) JP2002517981A (fr)
CA (1) CA2318371A1 (fr)
WO (1) WO1999036571A2 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8901287B2 (en) 2004-10-20 2014-12-02 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9410146B2 (en) 2009-09-14 2016-08-09 Qiagen Gaithersburg Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399589B2 (en) 1998-02-06 2008-07-15 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US6686151B1 (en) * 1998-02-06 2004-02-03 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
ATE316152T1 (de) 1998-10-27 2006-02-15 Affymetrix Inc Komplexitätsmanagement und analyse genomischer dna
US6692914B1 (en) 1999-07-02 2004-02-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate, where the polymers have water-soluble or water-dispersible segments and probes bonded thereto
EP1111069A1 (fr) * 1999-12-22 2001-06-27 BioChip Technologies GmbH Des acides nucléiques modifiés et leur utilisation
DK1132739T3 (da) * 2000-05-16 2002-01-28 Biochip Technologies Gmbh Linkersystem til aktivering af overflader til biokonjugation og fremgangsmåder til anvendelse deraf
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
JP2002176985A (ja) * 2000-12-14 2002-06-25 Hitachi Software Eng Co Ltd Ｐｃｒ増幅された塩基配列の検出方法及び検出キット
US7205161B2 (en) 2001-01-10 2007-04-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate having improved stability
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
ES2622062T3 (es) 2008-10-27 2017-07-05 Qiagen Gaithersburg, Inc. Ensayo y sistema de captura de híbrido de resultados rápidos
CA2760542A1 (fr) 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. Procede d'amplification non ciblee pour la detection de formes d'epissage d'arn dans un echantillon
CA2787924A1 (fr) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Procedes et compositions pour la purification et l'analyse multiplexee d'acides nucleiques sequence-specifique
CN102822353A (zh) 2010-01-29 2012-12-12 奇亚根盖瑟斯堡股份有限公司 确定和确认样品中存在hpv的方法
EP2678442B1 (fr) 2011-02-24 2019-01-16 QIAGEN Gaithersburg, Inc. Matériels et procédés de détection d'acide nucléique de hpv
US9315807B1 (en) 2013-01-26 2016-04-19 New England Biolabs, Inc. Genome selection and conversion method
US10087481B2 (en) 2013-03-19 2018-10-02 New England Biolabs, Inc. Enrichment of target sequences
JP6441893B2 (ja) 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
CN111979303A (zh) * 2020-08-11 2020-11-24 上海奕谱生物科技有限公司 一种核酸检测试剂盒、方法及其应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012695A1 (fr) * 1988-06-17 1989-12-28 Genelabs Incorporated Techniques d'amplification et de soustraction d'adn
EP0372524A2 (fr) * 1988-12-07 1990-06-13 The General Hospital Corporation Procédé pour l'enrichissement et le clonage d'ADN équivalent à une insertion ou une délétion
WO1990009457A2 (fr) * 1989-02-14 1990-08-23 Biogen, Inc. Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation
WO1995015974A1 (fr) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Acides nucleiques de protection et procedes d'analyse
WO1996036731A2 (fr) * 1995-05-19 1996-11-21 Trustees Of Boston University Procedes de detection d'acides nucleiques
WO1997019192A1 (fr) * 1995-11-20 1997-05-29 Trustees Of Boston University Procede et sonde servant a detecter une sequence cible d'acide nucleique
WO1997047640A1 (fr) * 1996-06-14 1997-12-18 Sarnoff Corporation Dosages avec protection par une nuclease

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012695A1 (fr) * 1988-06-17 1989-12-28 Genelabs Incorporated Techniques d'amplification et de soustraction d'adn
EP0372524A2 (fr) * 1988-12-07 1990-06-13 The General Hospital Corporation Procédé pour l'enrichissement et le clonage d'ADN équivalent à une insertion ou une délétion
WO1990009457A2 (fr) * 1989-02-14 1990-08-23 Biogen, Inc. Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation
WO1995015974A1 (fr) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Acides nucleiques de protection et procedes d'analyse
WO1996036731A2 (fr) * 1995-05-19 1996-11-21 Trustees Of Boston University Procedes de detection d'acides nucleiques
WO1997019192A1 (fr) * 1995-11-20 1997-05-29 Trustees Of Boston University Procede et sonde servant a detecter une sequence cible d'acide nucleique
WO1997047640A1 (fr) * 1996-06-14 1997-12-18 Sarnoff Corporation Dosages avec protection par une nuclease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG M ET AL: "CLONING DIFFERENTIALLY EXPRESSED GENES BY LINKER CAPTURE SUBTRACTION", ANALYTICAL BIOCHEMISTRY, vol. 237, no. 1, 15 May 1996 (1996-05-15), pages 109 - 114, XP000587696 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8901287B2 (en) 2004-10-20 2014-12-02 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9410146B2 (en) 2009-09-14 2016-08-09 Qiagen Gaithersburg Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes

Also Published As

Publication number Publication date
CA2318371A1 (fr) 1999-07-22
WO1999036571A2 (fr) 1999-07-22
EP1047794A2 (fr) 2000-11-02
JP2002517981A (ja) 2002-06-25

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