[go: up one dir, main page]

WO2000027365A8 - Functionalized nanocrystals and their use in detection systems - Google Patents

Functionalized nanocrystals and their use in detection systems

Info

Publication number
WO2000027365A8
WO2000027365A8 PCT/US1999/026487 US9926487W WO0027365A8 WO 2000027365 A8 WO2000027365 A8 WO 2000027365A8 US 9926487 W US9926487 W US 9926487W WO 0027365 A8 WO0027365 A8 WO 0027365A8
Authority
WO
WIPO (PCT)
Prior art keywords
functionalized nanocrystals
substrate
nanocrystals
functionalized
complexes
Prior art date
Application number
PCT/US1999/026487
Other languages
French (fr)
Other versions
WO2000027365A9 (en
WO2000027365A1 (en
Inventor
Emilio Barbera-Guillem
Stephanie Castro
Original Assignee
Biocrystal Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/372,729 external-priority patent/US6114038A/en
Application filed by Biocrystal Ltd filed Critical Biocrystal Ltd
Priority to AU21470/00A priority Critical patent/AU2147000A/en
Priority to EP99965776A priority patent/EP1128818A4/en
Publication of WO2000027365A1 publication Critical patent/WO2000027365A1/en
Publication of WO2000027365A8 publication Critical patent/WO2000027365A8/en
Publication of WO2000027365A9 publication Critical patent/WO2000027365A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
    • G01N23/223—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material by irradiating the sample with X-rays or gamma-rays and by measuring X-ray fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2223/00—Investigating materials by wave or particle radiation
    • G01N2223/07—Investigating materials by wave or particle radiation secondary emission
    • G01N2223/076—X-ray fluorescence

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nanotechnology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Materials Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Provided are compositions comprising water-soluble, functionalized nanocrystals. The water-soluble functionalized nanocrystals comprise quantum dots capped with a layer of a capping compound, and further comprise, by operably linking and in a successive manner, one or more additional compounds. Preferably, an additional compound comprises diaminocarboxylic acid which is operatively linked to the capping compound, and may further comprise an amino acid, and affinity ligand, or a combination thereof. Also provided are methods of using the functionalized nanocrystals having an affinity ligand to detect the presence or absence of a target substrate in a sample by contacting the functionalized nanocrystals with the sample so that complexes are formed between the functionalized nanocrystals and substrate, if the substrate is present; exposing the complexes in the detection system to an excitation light source, and detecting the emitted fluorescence peak.
PCT/US1999/026487 1998-11-10 1999-11-10 Functionalized nanocrystals and their use in detection systems WO2000027365A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU21470/00A AU2147000A (en) 1998-11-10 1999-11-10 Functionalized nanocrystals and their use in detection systems
EP99965776A EP1128818A4 (en) 1998-11-10 1999-11-10 Functionalized nanocrystals and their use in detection systems

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US60/109,626 1998-11-10
US10962698P 1998-11-24 1998-11-24
US09/372,729 1999-08-11
US09/372,729 US6114038A (en) 1998-11-10 1999-08-11 Functionalized nanocrystals and their use in detection systems

Publications (3)

Publication Number Publication Date
WO2000027365A1 WO2000027365A1 (en) 2000-05-18
WO2000027365A8 true WO2000027365A8 (en) 2000-09-14
WO2000027365A9 WO2000027365A9 (en) 2000-11-23

Family

ID=26807171

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/026487 WO2000027365A1 (en) 1998-11-10 1999-11-10 Functionalized nanocrystals and their use in detection systems

Country Status (1)

Country Link
WO (1) WO2000027365A1 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6607829B1 (en) 1997-11-13 2003-08-19 Massachusetts Institute Of Technology Tellurium-containing nanocrystalline materials
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6306610B1 (en) 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6617583B1 (en) 1998-09-18 2003-09-09 Massachusetts Institute Of Technology Inventory control
US6261779B1 (en) 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
US6602274B1 (en) 1999-01-15 2003-08-05 Light Sciences Corporation Targeted transcutaneous cancer therapy
US6454789B1 (en) 1999-01-15 2002-09-24 Light Science Corporation Patient portable device for photodynamic therapy
HK1049923A1 (en) 2000-03-14 2003-05-30 Massachusetts Institute Of Technology Optical amplifiers and lasers
DE60128458T2 (en) 2000-03-20 2008-01-10 Massachusetts Institute Of Technology, Cambridge INORGANIC PARTICLE CONJUGATES
WO2001090716A2 (en) * 2000-05-24 2001-11-29 Vanderbilt University Linker arms for nanocrystals and compounds thereof
CA2428732C (en) * 2000-11-15 2018-07-31 Minerva Biotechnologies Corporation Methods and kit for simultaneous screening of interactions between a plurality of chemical or biological species and uses thereof
US20020110180A1 (en) 2001-02-09 2002-08-15 Barney Alfred A. Temperature-sensing composition
WO2002073155A1 (en) * 2001-03-09 2002-09-19 Scientific Development Service Stroisnabservice Ultrasensitive non-isotopic water-soluble nanocrystals
GB0113772D0 (en) * 2001-06-06 2001-07-25 Dynal Biotech Asa Process
US7205048B2 (en) * 2001-09-17 2007-04-17 Invitrogen Corporation Functionalized fluorescent nanocrystal compositions and methods of making
JP4383865B2 (en) 2001-09-17 2009-12-16 マサチューセッツ・インスティテュート・オブ・テクノロジー Semiconductor nanocrystal composite
AU2002362013B2 (en) 2001-11-21 2008-04-24 Applied Biosystems, Llc. Digital assay
US7998923B2 (en) 2002-05-07 2011-08-16 The Regents Of The University Of California Bioactivation of particles
US7319709B2 (en) 2002-07-23 2008-01-15 Massachusetts Institute Of Technology Creating photon atoms
WO2004042784A2 (en) 2002-08-15 2004-05-21 Massachussetts Institute Of Technology Stabilized semiconductor nanocrystals
US7181266B2 (en) 2003-03-04 2007-02-20 Massachusetts Institute Of Technology Materials and methods for near-infrared and infrared lymph node mapping
US7917298B1 (en) 2003-04-17 2011-03-29 Nanosys, Inc. Nanocrystal taggants
WO2005024425A1 (en) * 2003-09-09 2005-03-17 Koninklijke Philips Electronics N.V. Nanoparticles for detecting analytes
US7943396B2 (en) 2004-06-22 2011-05-17 The Regents Of The University Of California Peptide-coated nanoparticles with graded shell compositions
US7229690B2 (en) 2004-07-26 2007-06-12 Massachusetts Institute Of Technology Microspheres including nanoparticles
US20110129944A1 (en) * 2005-01-17 2011-06-02 Agency For Science, Technology And Research Water-soluble nanocrystals and methods of preparing them
EP1883820A4 (en) * 2005-05-04 2010-06-16 Agency Science Tech & Res Novel water-soluble nanocrystals comprising a low molecular weight coating reagent, and methods of preparing the same
WO2007120762A2 (en) * 2006-04-14 2007-10-25 Cambrios Technologies Corporation Fluorescent particles bound to multifunctional scaffolds and their uses
WO2008070459A2 (en) * 2006-11-22 2008-06-12 Alnis Biosciences, Inc. Coated nanoparticles for aqueous systems
GB0813273D0 (en) * 2008-07-19 2008-08-27 Nanoco Technologies Ltd Method for producing aqueous compatible nanoparticles

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048616A (en) * 1993-04-21 2000-04-11 Philips Electronics N.A. Corp. Encapsulated quantum sized doped semiconductor particles and method of manufacturing same
WO1996014206A1 (en) * 1994-11-08 1996-05-17 Spectra Science Corporation Semiconductor nanocrystal display materials and display apparatus employing same
US5908608A (en) * 1996-11-08 1999-06-01 Spectra Science Corporation Synthesis of metal chalcogenide quantum
JPH1187689A (en) * 1997-09-04 1999-03-30 Fujitsu Ltd Manufacturing method of quantum dots
JP2980175B2 (en) * 1997-11-20 1999-11-22 日本電気株式会社 Method for manufacturing quantum dot structure and method for manufacturing semiconductor light emitting device using the same
US5990479A (en) * 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes

Also Published As

Publication number Publication date
WO2000027365A9 (en) 2000-11-23
WO2000027365A1 (en) 2000-05-18

Similar Documents

Publication Publication Date Title
WO2000027365A8 (en) Functionalized nanocrystals and their use in detection systems
AU2001237057A1 (en) Functionalized encapsulated fluorescent nanocrystals
CA2069145A1 (en) Assay method utilizing induced luminescence
US6770220B1 (en) Production and use of luminescent microparticles and nanoparticles
US4374120A (en) Fluorescence spectroscopy assay means with fluorescent chelate of a lanthanide
WO2003060461A3 (en) System and method for fluorescence detection
WO2001086299A3 (en) Doped nanoparticles as biolabels
WO2001029532A3 (en) Fluorescence filter cube for fluorescence detection and imaging
GR3018429T3 (en) Method for amplifying a signal emitted by a luminescent compound.
EP1418419A3 (en) A high efficiency fluorescence flow cell
CA2433211A1 (en) Determining dew precipitation and onset pressure in oilfield retrograde condensate
EP2248912B8 (en) High sensitivity assays for pathogen detection using metal-enhanced fluorescence
ATE270298T1 (en) REACTION SYSTEM AND METHOD FOR ENHANCING THE LUMINESCENCE OF MACROCYCLIC LANTHANIDE(III) COMPLEXES
ATE433498T1 (en) DETECTION OF NEOPLASIA BY SALIVA ANALYSIS
AU4701200A (en) A method of detecting an analyte using semiconductor nanocrystals
EP0770969A3 (en) System and method of improving the signal to noise ratio of bar code and indicia scannners that utilize fluorescent inks
AU2001289853A1 (en) Multianalyte determination system and methods
AU2001269256A1 (en) Electrochemical immunoassays using colloidal metal markers
WO2001044811A3 (en) Plasminogen activator assay involving an elastase inhibitor
WO2002068932A3 (en) Methods for providing extended dynamic range in analyte assays
WO2003062795A3 (en) Methods for fluorescence detection that minimizes undesirable background fluorescence
CN103115905A (en) Fluorescence detection probe for lead ions
WO2002054067A3 (en) Detection of analytes
WO2003096014A3 (en) Magneto-controlled method and system for determining an analyte in a liquid medium
WO2001096862A3 (en) Method for increasing luminescence assay sensitivity

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: C1

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: C1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: PAT. BUL. 20/2000 UNDER (30) REPLACE "NOT FURNISHED" BY "09/372729"

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: C2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/3-3/3, DRAWINGS, REPLACED BY NEW PAGES 1/3-3/3; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

WWE Wipo information: entry into national phase

Ref document number: 1999965776

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999965776

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 1999965776

Country of ref document: EP