[go: up one dir, main page]

WO2003033530A3 - Antigenes mycobacteriens exprimes a haute tension en oxygene - Google Patents

Antigenes mycobacteriens exprimes a haute tension en oxygene Download PDF

Info

Publication number
WO2003033530A3
WO2003033530A3 PCT/GB2002/004647 GB0204647W WO03033530A3 WO 2003033530 A3 WO2003033530 A3 WO 2003033530A3 GB 0204647 W GB0204647 W GB 0204647W WO 03033530 A3 WO03033530 A3 WO 03033530A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes
induced
mycobacterial
oxygen tension
identifying
Prior art date
Application number
PCT/GB2002/004647
Other languages
English (en)
Other versions
WO2003033530A2 (fr
Inventor
Brian James
Joanna Bacon
Philip March
Original Assignee
Health Prot Agency
Brian James
Joanna Bacon
Philip March
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Health Prot Agency, Brian James, Joanna Bacon, Philip March filed Critical Health Prot Agency
Priority to AU2002337287A priority Critical patent/AU2002337287A1/en
Publication of WO2003033530A2 publication Critical patent/WO2003033530A2/fr
Publication of WO2003033530A3 publication Critical patent/WO2003033530A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention concerne un procédé permettant d'identifier des gènes mycobactériens dont l'expression est induite ou régulée positivement dans des conditions de culture continue caractérisées par une tension en oxygène dissous d'au moins 30 % de saturation en air mesurée à 37 °C, comparée à une tension en oxygène dissous allant jusqu'à 10 % de saturation en air, mesurée à 37 °C. Le procédé selon l'invention permet facultativement d'identifier des gènes mycobactériens simultanément induits ou régulés positivement dans des conditions de culture continue limitant le fer. Lesdits gènes induits ou régulés positivement forment la base de vaccins d'acides nucléiques ou constituent des cibles permettant de préparer des mycobactéries atténuées destinées à des vaccins contre les infections mycobactériennes. De même, les peptides codés par lesdits gènes induits ou régulés positivement sont utilisés dans des vaccins. Dans un autre mode de réalisation, les gènes/peptides identifiés constituent un moyen permettant d'identifier la présence d'une infection mycobactérienne dans un échantillon clinique, au moyen d'une sonde d'acide nucléique ou d'une détection d'anticorps.
PCT/GB2002/004647 2001-10-12 2002-10-14 Antigenes mycobacteriens exprimes a haute tension en oxygene WO2003033530A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002337287A AU2002337287A1 (en) 2001-10-12 2002-10-14 Mycobacterial antigens expressed under high oxygen tension

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0124593.5A GB0124593D0 (en) 2001-10-12 2001-10-12 Mycobacterial antigens expressed under high oxygen tension
GB0124593.5 2001-10-12

Publications (2)

Publication Number Publication Date
WO2003033530A2 WO2003033530A2 (fr) 2003-04-24
WO2003033530A3 true WO2003033530A3 (fr) 2004-02-26

Family

ID=9923760

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/004647 WO2003033530A2 (fr) 2001-10-12 2002-10-14 Antigenes mycobacteriens exprimes a haute tension en oxygene

Country Status (3)

Country Link
AU (1) AU2002337287A1 (fr)
GB (1) GB0124593D0 (fr)
WO (1) WO2003033530A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ519395A (en) * 2002-06-07 2004-04-30 Agres Ltd Techniques for identifying genes that are important for virulence in Mycobacterial vaccines
US7608277B2 (en) * 2004-12-01 2009-10-27 Gene Therapy Systems, Inc. Tuberculosis nucleic acids, polypeptides and immunogenic compositions
EP1910410B1 (fr) * 2005-07-01 2011-10-26 Forsyth Dental Infirmary for Children Essais de detection d'antigenes de la tuberculose et vaccins
WO2007087679A1 (fr) * 2006-01-31 2007-08-09 Proteome Systems Limited Procédé de diagnostic et de traitement d'une infection à m. tuberculosis et réactifs correspondants
EP2441493B1 (fr) * 2006-03-14 2014-05-07 Oregon Health and Science University Méthode pour produire une reponse contre la tuberculose
CN102713629B (zh) * 2009-11-20 2016-02-24 俄勒冈健康科学大学 用于检测结核分枝杆菌感染的方法
CN103122364A (zh) * 2011-11-21 2013-05-29 华中农业大学 抗结核抑制剂筛选的靶标基因Rv2498c及应用
CN104419710B (zh) * 2013-09-04 2017-06-16 上海市肺科医院 一种结核分枝杆菌的耐药新基因及其应用
US10213766B2 (en) 2015-09-18 2019-02-26 Chevron Phillips Chemical Company Lp Methods of preparing a catalyst

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
COLER ET AL: "Vaccination with the T cell antigen Mtb 8.4 protects against challenge with Mycobacterium tuberculosis", JOURNAL OF IMMUNOLOGY, vol. 166, May 2001 (2001-05-01), pages 6227 - 6235, XP002961490, ISSN: 0022-1767 *
DATABASE EMBL [online] 1 January 1998 (1998-01-01), "Acyl carrier protein", XP002250930, retrieved from EBI accession no. P71603 Database accession no. P71603 *
DATABASE EMBL [online] 1 June 1998 (1998-06-01), "Putative maturase homolog", XP002250931, retrieved from EBI accession no. O53616 Database accession no. O53616 *
IMBODEN ET AL: "Construction and characterization of a partial Myco- bacterium tuberculosis cDNA library of genes expressed at reduced oxygen tension", GENE, vol. 213, no. 1-2, 1998, pages 107 - 117, XP004125009, ISSN: 0378-1119 *
JAMES ET AL: "The physiology and pathogenicity of Mycobacterium tuberculosis grown under controlled conditions in a defined medium", JOURNAL OF APPLIED MICROBIOLOGY, vol. 88, no. 4, 2000, pages 669 - 677, XP000929200, ISSN: 1364-5072 *
PEREZ-GUZMAN: "Progressive age-related changes in pulmonary tuberculosis images and the effect of diabetes", AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, vol. 162, 2000, pages 1738 - 1740, XP002250929 *
WONG ET AL: "Identification of fur, aconitase, and other proteins expressed by Mycobacterium tuberculosis under conditions of low and high concentrations of iron by combined two-dimensional gel electrophoresis and mass spectrometry", INFECTION AND IMMUNITY, vol. 67, no. 1, 1999, pages 327 - 336, XP002250955 *

Also Published As

Publication number Publication date
WO2003033530A2 (fr) 2003-04-24
AU2002337287A1 (en) 2003-04-28
GB0124593D0 (en) 2001-12-05

Similar Documents

Publication Publication Date Title
WO2003000721A3 (fr) Antigenes mycobacteriens exprimes sous faible tension en oxygene
WO2003004520A3 (fr) Antigenes mycobacteriens exprimes pendant la latence
AR054477A1 (es) Vacunas para inmunizacion contra helicobacter
CA2365898A1 (fr) Antigenes microbiens associes aux affections intestinales inflammatoires et procedes pour l'utilisation de ces antigenes
EP3118326A3 (fr) Marqueur moléculaire de cellule souche cancéreuse
CA2412121A1 (fr) Methode de detection des virus de la grippe a et/ou b
WO2007030391A3 (fr) Procede de diagnostic d'infections
WO2003033530A3 (fr) Antigenes mycobacteriens exprimes a haute tension en oxygene
Shil et al. GapA+ Mycoplasma gallisepticum ts-11 has improved vaccine characteristics
WO2003095968A3 (fr) Procede de detection simultanee d'un antigene et d'un anticorps d'un microorganisme infectueux
WO2001066568A3 (fr) Utilisation d'antigenes recombines pour determiner l'etat immunitaire d'un animal
Ahmad et al. The first isolation and molecular characterization of Mycoplasma capricolum subsp. capripneumoniae Pakistan strain: A causative agent of contagious caprine pleuropneumonia
WO2003035681A3 (fr) Genes mycobacteriens retro-regules au cours de la latence
JP5835702B2 (ja) クリプトコックス症起因菌の検出法と検出キット
WO2012051372A3 (fr) Antigènes immunoréactifs de mycoplasma haemofelis et immunoessai diagnostique
DE60319121D1 (de) Eines antigens auf einer zelle und in einem biologischen gemisch
Mukherjee et al. Optimization and validation of a diagnostic real-time PCR for bovine brucellosis
WO2004083448A3 (fr) Differences moleculaires entre des especes du complexe m. tuberculosis
EP2508201A1 (fr) Procédé d'identification d'animaux vaccinés contre brucella
ÖZKUL et al. Comparison of four diagnostic techniques for detecting bovine virus diarrhoea virus (BVDV) in buffy coat samples after long-term storage
WO2005003376A3 (fr) Essai immunologique rapide d'antigene protecteur contre l'anthrax dans des cultures vaccinales par polarisation a fluorescence
WO2004029216A3 (fr) Diagnostic d'une infection a moisissures invasives
CN101900727A (zh) 一种应用Rv3872新型融合蛋白制备的牛结核抗体鉴别检测试纸条
CN103146836B (zh) 鉴定和检测多种羊泰勒虫的试剂盒及制备方法
Lee et al. Burkholderia pseudomallei: an update on disease, virulence and host interaction.

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BY BZ CA CH CN CO CR CU CZ DE DM DZ EC EE ES FI GB GD GE GH HR HU ID IL IN IS JP KE KG KP KR LC LK LR LS LT LU LV MA MD MG MN MW MX MZ NO NZ OM PH PL PT RU SD SE SG SI SK SL TJ TM TN TR TZ UA UG US UZ VC VN YU ZA ZM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ UG ZM ZW AM AZ BY KG KZ RU TJ TM AT BE BG CH CY CZ DK EE ES FI FR GB GR IE IT LU MC PT SE SK TR BF BJ CF CG CI GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP