[go: up one dir, main page]

WO2006045590A3 - Molecules destinees a l'administration d'arn en brin double a des organismes de parasites - Google Patents

Molecules destinees a l'administration d'arn en brin double a des organismes de parasites Download PDF

Info

Publication number
WO2006045590A3
WO2006045590A3 PCT/EP2005/011439 EP2005011439W WO2006045590A3 WO 2006045590 A3 WO2006045590 A3 WO 2006045590A3 EP 2005011439 W EP2005011439 W EP 2005011439W WO 2006045590 A3 WO2006045590 A3 WO 2006045590A3
Authority
WO
WIPO (PCT)
Prior art keywords
multidomain
aptamer
stranded rna
rna
nucleotide sequence
Prior art date
Application number
PCT/EP2005/011439
Other languages
English (en)
Other versions
WO2006045590A2 (fr
Inventor
Thierry Andre Olivier Bogaert
Pascale Feldmann
Geert Plaetinck
Original Assignee
Devgen Nv
Thierry Andre Olivier Bogaert
Pascale Feldmann
Geert Plaetinck
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Devgen Nv, Thierry Andre Olivier Bogaert, Pascale Feldmann, Geert Plaetinck filed Critical Devgen Nv
Priority to EP05801497A priority Critical patent/EP1805308A2/fr
Priority to CA002583334A priority patent/CA2583334A1/fr
Priority to MX2007004686A priority patent/MX2007004686A/es
Priority to BRPI0516329-3A priority patent/BRPI0516329A/pt
Priority to AU2005298827A priority patent/AU2005298827B2/en
Publication of WO2006045590A2 publication Critical patent/WO2006045590A2/fr
Publication of WO2006045590A3 publication Critical patent/WO2006045590A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne des procédés et des produits de synthèse permettant d'administrer un ARN en double brin (RNAdb) à des organismes de parasites. Plus précisément, l'invention concerne une molécule d'ARN multidomaine comprenant une séquence nucléotidique renfermant : (i) au moins un aptamère et (ii) au moins une séquence nucléotidique étudiée formant un ARN en brin double, celui-ci comprenant des brins complémentaires recuits, un de ceux-ci présentant une séquence nucléotidique complémentaire d'au moins partiellement une séquence nucléotidique cible de parasites. L'invention concerne également des acides nucléiques codant les molécules d'ARN multidomaine et diverses utilisations des molécules d'ARN multidomaine en agriculture.
PCT/EP2005/011439 2004-10-25 2005-10-25 Molecules destinees a l'administration d'arn en brin double a des organismes de parasites WO2006045590A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP05801497A EP1805308A2 (fr) 2004-10-25 2005-10-25 Arn à domaines multiples comprenant au moins un aptamère pour délivrer arn à double brin dans un organisme parasite
CA002583334A CA2583334A1 (fr) 2004-10-25 2005-10-25 Molecules destinees a l'administration d'arn en brin double a des organismes de parasites
MX2007004686A MX2007004686A (es) 2004-10-25 2005-10-25 Moleculas de arn de multidominio que comprenden al menos un aptamero para suministrar arn de doble hebra a organismos nocivos.
BRPI0516329-3A BRPI0516329A (pt) 2004-10-25 2005-10-25 moléculas de rna de multidomìnio que compreendem pelo menos um aptámero para a aplicação de rna de cordão duplo a organismos de pestes
AU2005298827A AU2005298827B2 (en) 2004-10-25 2005-10-25 Multidomain RNA molecules comprising at least one aptamer for delivering double stranded RNA to pest organisms

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US62180104P 2004-10-25 2004-10-25
US60/621,801 2004-10-25
EP04447235 2004-10-25
EP04447235.5 2004-10-25
US62902704P 2004-11-18 2004-11-18
US60/629,027 2004-11-18
EP04447251 2004-11-18
EP04447251.2 2004-11-18

Publications (2)

Publication Number Publication Date
WO2006045590A2 WO2006045590A2 (fr) 2006-05-04
WO2006045590A3 true WO2006045590A3 (fr) 2006-06-15

Family

ID=56290744

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/011439 WO2006045590A2 (fr) 2004-10-25 2005-10-25 Molecules destinees a l'administration d'arn en brin double a des organismes de parasites

Country Status (6)

Country Link
EP (1) EP1805308A2 (fr)
AU (1) AU2005298827B2 (fr)
BR (1) BRPI0516329A (fr)
CA (1) CA2583334A1 (fr)
MX (1) MX2007004686A (fr)
WO (1) WO2006045590A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946510B2 (en) 2004-04-09 2015-02-03 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7612194B2 (en) 2001-07-24 2009-11-03 Monsanto Technology Llc Nucleic acid sequences from Diabrotica virgifera virgifera LeConte and uses thereof
US20050256071A1 (en) * 2003-07-15 2005-11-17 California Institute Of Technology Inhibitor nucleic acids
CA2622671C (fr) 2005-09-16 2020-12-22 Devgen Nv Procede de protection phytosanitaire a base d'interference arn
CN101310020A (zh) 2005-09-16 2008-11-19 孟山都技术有限公司 用于在植物中遗传控制昆虫侵袭的方法和组合物
CN101489383A (zh) * 2006-06-01 2009-07-22 杜克大学 传递方法
US8367895B2 (en) 2008-01-17 2013-02-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from the family aphididae
US8809625B2 (en) 2008-01-17 2014-08-19 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from Lygus
US8847013B2 (en) 2008-01-17 2014-09-30 Pioneer Hi Bred International Inc Compositions and methods for the suppression of target polynucleotides from lepidoptera
BR112012030913A2 (pt) 2010-06-04 2015-09-08 Du Pont polinucleotídeo isolado, cassete de expressão, célula hospedeira, célula de planta, planta ou parte de planta, semente transgênica e método para controlar uma praga de plantas pentatomidae.
PL3116312T3 (pl) 2013-12-20 2020-01-31 The University Of Queensland Kompozycje RNAi chroniące rośliny zawierające dwuniciowe RNA chroniące rośliny zaadsorbowane na cząsteczkach warstwowych podwójnych wodorotlenków
CN108024542B (zh) 2015-06-19 2021-05-14 昆士兰大学 组合物
CN112899301B (zh) * 2021-01-29 2023-04-11 中国热带农业科学院热带生物技术研究所 一种木薯普通花叶病毒诱导的基因沉默系统及其应用

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DIECKMANN THORSTEN ET AL: "Solution structure of an ATP-binding RNA aptamer reveals a novel fold", RNA (NEW YORK), vol. 2, no. 7, 1996, pages 628 - 640, XP002325933, ISSN: 1355-8382 *
FAROKHZAD OMID C ET AL: "Nanoparticle-aptamer bioconjugates: a new approach for targeting prostate cancer cells.", CANCER RESEARCH. 1 NOV 2004, vol. 64, no. 21, 1 November 2004 (2004-11-01), pages 7668 - 7672, XP002325935, ISSN: 0008-5472 *
LATO SUSAN M ET AL: "Boron-containing aptamers to ATP.", NUCLEIC ACIDS RESEARCH. 15 MAR 2002, vol. 30, no. 6, 15 March 2002 (2002-03-15), pages 1401 - 1407, XP002325934, ISSN: 1362-4962 *
NISHIKAWA F ET AL: "Inhibition of HCV protease by RNA aptamers in cells", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 31, no. 7, 1 April 2003 (2003-04-01), pages 1935 - 1943, XP002260526, ISSN: 0305-1048 *
SCHERR M ET AL: "GENE SILENCING MEDIATED BY SMALL INTERFERING RNAS IN MAMMALIAN CELLS", CURRENT MEDICINAL CHEMISTRY, BENTHAM SCIENCE PUBLISHERS BV, BE, vol. 10, no. 3, February 2003 (2003-02-01), pages 245 - 256, XP009019025, ISSN: 0929-8673 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946510B2 (en) 2004-04-09 2015-02-03 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
US9238822B2 (en) 2004-04-09 2016-01-19 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
US9340797B2 (en) 2004-04-09 2016-05-17 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants

Also Published As

Publication number Publication date
AU2005298827A1 (en) 2006-05-04
AU2005298827B2 (en) 2011-09-08
WO2006045590A2 (fr) 2006-05-04
MX2007004686A (es) 2007-06-14
CA2583334A1 (fr) 2006-05-04
BRPI0516329A (pt) 2008-03-11
EP1805308A2 (fr) 2007-07-11

Similar Documents

Publication Publication Date Title
WO2004015107A3 (fr) Nouvelles formes de molecules d'arn interferant
WO2004007718A3 (fr) Interference de l'arn (arni) par des molecules d'arn simple brin
WO2003070903A3 (fr) Inhibition a mediation par l'interference par l'arn de l'expression genique de la proteine de liaison 2 associee a grb2 (gab2) au moyen de petits fragments d'acides nucleiques interferants (sina)
WO2011056005A3 (fr) Nouvelle structure d'arnsi pour réduire au minimum les effets hors cible causés par des brins antisens, et utilisation de celle-ci
JP2005517432A5 (fr)
WO2006045590A3 (fr) Molecules destinees a l'administration d'arn en brin double a des organismes de parasites
EP3012324A3 (fr) Modulation de l'expression hsp47
WO2005121372A3 (fr) Compositions a double brin comprenant des brins differentiellement modifies utilises dans la modulation genetique
NO20053465L (no) Pavisning av sma nukleinsyrer
WO2006036739A3 (fr) Extinction genique
WO2004065600A3 (fr) Interference d'arn par des molecules d'arn palindromiques et marquees
JP2005517433A5 (fr)
JP2005517438A5 (fr)
WO2007083193A3 (fr) Procédé de protection phytosanitaire à base d'interférence arn
EP3358014A3 (fr) Procédé de stabilisation d'acides nucléiques fonctionnels
WO2007048628A3 (fr) Structures de molecules d'arn-guide actives et leur procede de selection
WO2003070887A3 (fr) Inhibition mediee par interference d'arn de l'expression du gene de la proteine ezh2 du groupe polycomb a l'aide d'un acide nucleique court interferent (sina)
WO2005054438A3 (fr) Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
WO2006125447A3 (fr) Formation stable et selective de triplexes et de duplexes de type hoogsteen au moyen d'acides nucleiques intercalants tordus (tina) et procede de preparation de tina
WO2012078949A3 (fr) Procédés et compositions utilisant un petit arn interférant (arnsi) pour la lutte contre les nématodes chez les plantes
WO2005110067A3 (fr) Procede et compositions destines a l'interference d'arn
WO2005021800A3 (fr) Detection et quantification de molecules d'acide nucleique dans des echantillons biologiques
WO2010017311A3 (fr) Composés d’acide nucléique pour inhiber l’expression du gène birc5 et utilisations de ceux-ci
WO2004097020A3 (fr) Inhibition induite par interference arn de l'expression genique d'une map kinase au moyen d'acide nucleique interferant court (sina)
WO2005045041A3 (fr) Inhibition de l'expression du gene de la proteine de transfert des esters de cholesterol (cetp) induite par interference arn, a l'aide de petits acides nucleiques interferents (sina)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BW BY BZ CA CH CN CO CR CU CZ DK DM DZ EC EE EG ES FI GB GD GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV LY MD MG MK MN MW MX MZ NA NG NO NZ OM PG PH PL PT RO RU SC SD SG SK SL SM SY TJ TM TN TR TT TZ UG US UZ VC VN YU ZA ZM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SZ TZ UG ZM ZW AM AZ BY KG MD RU TJ TM AT BE BG CH CY DE DK EE ES FI FR GB GR HU IE IS IT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2583334

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2005298827

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2007/03064

Country of ref document: ZA

WWE Wipo information: entry into national phase

Ref document number: MX/a/2007/004686

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 200580036592.1

Country of ref document: CN

Ref document number: 3103/DELNP/2007

Country of ref document: IN

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2005801497

Country of ref document: EP

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWP Wipo information: published in national office

Ref document number: 2005801497

Country of ref document: EP

ENP Entry into the national phase

Ref document number: PI0516329

Country of ref document: BR