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WO2006002841A3 - Analysis method - Google Patents

Analysis method Download PDF

Info

Publication number
WO2006002841A3
WO2006002841A3 PCT/EP2005/006866 EP2005006866W WO2006002841A3 WO 2006002841 A3 WO2006002841 A3 WO 2006002841A3 EP 2005006866 W EP2005006866 W EP 2005006866W WO 2006002841 A3 WO2006002841 A3 WO 2006002841A3
Authority
WO
WIPO (PCT)
Prior art keywords
mass
terminal
labelled peptide
internal standard
peptide internal
Prior art date
Application number
PCT/EP2005/006866
Other languages
French (fr)
Other versions
WO2006002841A2 (en
Inventor
Fredrik Nilsson
Original Assignee
Bioinvent Int Ab
Fredrik Nilsson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioinvent Int Ab, Fredrik Nilsson filed Critical Bioinvent Int Ab
Priority to CA002611596A priority Critical patent/CA2611596A1/en
Priority to JP2007517213A priority patent/JP2008503738A/en
Priority to EP05772836A priority patent/EP1759213A2/en
Publication of WO2006002841A2 publication Critical patent/WO2006002841A2/en
Publication of WO2006002841A3 publication Critical patent/WO2006002841A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for quantitative measurement using mass spectroscopy of a peptide or peptides generated from one or multiple analyte proteins by enzymatic or chemical cleavage, the method comprising the step of exposing together the sample to be analysed and one or multiple mass-labelled peptide internal standard precursors to enzymatic or chemical cleavage so as to generate from said precursor one or multiple mass-labelled peptide internal standards, wherein the mass-labelled peptide internal standard precursor comprises an extension of a least one amino acid N-terminal to the N-terminus of the N-terminal-most mass-labelled peptide internal standard generated therefrom, and an extension of at least one amino acid C-terminal to the C-terminus of the C-terminal-most mass-labelled peptide internal standard generated therefrom.
PCT/EP2005/006866 2004-06-25 2005-06-22 Analysis method WO2006002841A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002611596A CA2611596A1 (en) 2004-06-25 2005-06-22 Analysis method
JP2007517213A JP2008503738A (en) 2004-06-25 2005-06-22 Analysis method
EP05772836A EP1759213A2 (en) 2004-06-25 2005-06-22 Analysis method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0414233.7 2004-06-25
GB0414233A GB0414233D0 (en) 2004-06-25 2004-06-25 Analysis method

Publications (2)

Publication Number Publication Date
WO2006002841A2 WO2006002841A2 (en) 2006-01-12
WO2006002841A3 true WO2006002841A3 (en) 2006-04-20

Family

ID=32800164

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/006866 WO2006002841A2 (en) 2004-06-25 2005-06-22 Analysis method

Country Status (6)

Country Link
EP (1) EP1759213A2 (en)
JP (1) JP2008503738A (en)
AU (1) AU2005259524A1 (en)
CA (1) CA2611596A1 (en)
GB (1) GB0414233D0 (en)
WO (1) WO2006002841A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1736480B9 (en) * 2005-06-02 2009-03-04 Polyquant GmbH Artificial protein, method for absolute quantification of proteins and uses thereof
BRPI0709374A2 (en) * 2006-03-23 2011-07-12 Guzman Breyer Emelita De apolipoprotein fingerprinting technique and related methods
JP4913587B2 (en) * 2006-12-28 2012-04-11 雪印メグミルク株式会社 Peptide and protein quantification
WO2008146100A1 (en) * 2007-06-01 2008-12-04 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for absolute quantification of polypeptides
JP5999699B2 (en) * 2012-11-16 2016-09-28 国立研究開発法人理化学研究所 Protein quantification method
CN106556651B (en) * 2015-09-30 2019-06-07 深圳翰宇药业股份有限公司 The method for detecting alanine, lysine, glutamic acid and/or tyrosine
GB201521903D0 (en) * 2015-12-11 2016-01-27 Electrophoretics Ltd Isorbaric mass labels
EP3196646B1 (en) * 2016-01-19 2019-12-18 Hexal AG Methods of mapping protein variants

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003016861A2 (en) * 2001-08-14 2003-02-27 President And Fellows Of Harvard College Absolute quantification of proteins and modified forms thereof by multistage mass spectrometry
WO2003046148A2 (en) * 2001-11-29 2003-06-05 Thermo Finnigan Llc Polypeptide quantitation
US20040005633A1 (en) * 2001-03-22 2004-01-08 Joel Vandekerckhove Methods and apparatuses for gel-free qualitative and quantitative proteome analysis, and uses therefore
WO2005116660A2 (en) * 2004-05-25 2005-12-08 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods for making and using mass tag standards for quantitative proteomics

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040005633A1 (en) * 2001-03-22 2004-01-08 Joel Vandekerckhove Methods and apparatuses for gel-free qualitative and quantitative proteome analysis, and uses therefore
WO2003016861A2 (en) * 2001-08-14 2003-02-27 President And Fellows Of Harvard College Absolute quantification of proteins and modified forms thereof by multistage mass spectrometry
WO2003046148A2 (en) * 2001-11-29 2003-06-05 Thermo Finnigan Llc Polypeptide quantitation
WO2005116660A2 (en) * 2004-05-25 2005-12-08 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods for making and using mass tag standards for quantitative proteomics

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BARNIDGE DAVID R ET AL: "Evaluation of a cleavable stable isotope labeled synthetic peptide for absolute protein quantification using LC-MS/MS", JOURNAL OF PROTEOME RESEARCH, vol. 3, no. 3, May 2004 (2004-05-01), pages 658 - 661, XP002360371, ISSN: 1535-3893 *
KRIJGSVELD J ET AL: "Quantitative proteomics by metabolic labeling with stable isotopes", DRUG DISCOVERY TODAY: TARGETS 2004 UNITED KINGDOM, vol. 3, no. 2 SUPPL.1, 2004, pages S11 - S15, XP002360372, ISSN: 1741-8372 *
LIU RUTAO ET AL: "Structural reorganization of the transferrin C-lobe and transferrin receptor upon complex formation: The C-lobe binds to the receptor helical domain.", BIOCHEMISTRY, vol. 42, no. 43, 4 November 2003 (2003-11-04), pages 12447 - 12454, XP002360384, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
CA2611596A1 (en) 2006-01-12
JP2008503738A (en) 2008-02-07
EP1759213A2 (en) 2007-03-07
WO2006002841A2 (en) 2006-01-12
GB0414233D0 (en) 2004-07-28
AU2005259524A1 (en) 2006-01-12

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