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WO2008146960A1 - Bacillus clausii bc7 10863p capable d'éliminer les mauvaises odeurs et les métaux lourds, et procédés de clarification l'utilisant pour récupérer des ressources à partir de rebuts alimentaires ou d'eaux usées d'élevage - Google Patents

Bacillus clausii bc7 10863p capable d'éliminer les mauvaises odeurs et les métaux lourds, et procédés de clarification l'utilisant pour récupérer des ressources à partir de rebuts alimentaires ou d'eaux usées d'élevage Download PDF

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Publication number
WO2008146960A1
WO2008146960A1 PCT/KR2007/002221 KR2007002221W WO2008146960A1 WO 2008146960 A1 WO2008146960 A1 WO 2008146960A1 KR 2007002221 W KR2007002221 W KR 2007002221W WO 2008146960 A1 WO2008146960 A1 WO 2008146960A1
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WO
WIPO (PCT)
Prior art keywords
kccm
bacillus
food waste
clausii
megaterium
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Application number
PCT/KR2007/002221
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English (en)
Inventor
Gun Ho Choi
Kil Ho Choi
Original Assignee
Pyung Kang Special Vehicle Co., Ltd.
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Publication date
Application filed by Pyung Kang Special Vehicle Co., Ltd. filed Critical Pyung Kang Special Vehicle Co., Ltd.
Priority to PCT/KR2007/002221 priority Critical patent/WO2008146960A1/fr
Priority to KR20097025245A priority patent/KR101106033B1/ko
Publication of WO2008146960A1 publication Critical patent/WO2008146960A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/40—Treatment of liquids or slurries
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/70—Controlling the treatment in response to process parameters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/085—Bacillus cereus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/11—Bacillus megaterium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/141—Feedstock
    • Y02P20/145—Feedstock the feedstock being materials of biological origin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Definitions

  • the present invention relates to a novel microorganism species useful for reducing nasty odors and heavy metals in livestock wastewater and food waste. Also, the present invention is concerned with the use of this novel microorganism in converting livestock wastewater and food waste into useful resources.
  • the present invention relates to a novel microorganism species capable of removing nasty odors and heavy metals from livestock Wcistewater and food waste, and to a method for the production of organic liquid fertilizer from livestock Wcistewater, and to a method for the production of animal feeds from food waste.
  • methyl mercaptan provides an odor of rotten onions .
  • Hydrogen sulfide is an irritative, suffocative and colorless gas, which also gives off the smell of rotten onions.
  • Ammonia an irritative and colorless gas, is responsible for the characteristic urine smell. In combination, they give out a complex foul smell, disgusting persons and inhibiting the growth of the livestock.
  • Oxygenation oxygenates and degrades malodor ingredients and sterilizes bacteria, fungi and viruses so as to fundamentally remove odor resources .
  • Many methods naive been suggested for oxygenation.
  • Most chemical oxygenation methods use chlorine dioxide, sodium hypochlorite, or chlorate dioxide.
  • Enzymatic degradation methods are known to use deodorizers prepared from vegetable extracts, but their ingredients and operation principles are not disclosed.
  • Absorption methods based on absorbents with large surface areas, such as active carbon, decrease in efficiency with time because available surface areas of the absorbents drop after being in use for a period of time.
  • Masking methods in which natural or synthetic odorous compounds are dissolved in highly volatile solvents, such as methyl alcohol, to generate a strong odor which conceals a deadly odor, can solve the nasty odor problem temporarily, but cannot provide a fundamental solution thereto.
  • ozone or UV radiation is used alone or in combination as a deodorizing agent. Ozone, although useful for sterilization and deodorization, may itself act as a pollutant.
  • a photocatalyst such as Ti ⁇ 2
  • ozone a method in which a photocatalyst, such as Ti ⁇ 2 , is used, in combination with ozone, to degrade odor sources.
  • a limitation is imposed on an increase in the surface area of photocatalysts, such as Ti ⁇ 2.
  • sulfides such as hydrogen sulfide (H 2 S) , methyl mercaptan (CH 3 SH) , methyl sulfide
  • BTXs including benzenes, toluene and xylene
  • VOCs volatile organic compounds
  • VOCs volatile organic chemicals
  • An incineration method produces secondary air pollution while an absorption method is economically disadvantageous due to the use of active carbon.
  • chemical methods require a low expense for the construction of facilities therefor, but a high expense for the operation thereof.
  • biological methods although suffering from the disadvantage of requiring high expenses for the construction of facilities therefor and complicated conditions for the operation thereof, are advantageous in that they are easy to maintain and manage and can be operated at low cost.
  • a biofilter method typical of biological deodorization, uses a bioreactor loaded with a carrier onto which deodorizing microorganisms are immobilized. The lowest operation cost is found in the biofilter system. Operation costs increase in the order of biofiltration, chemical cleaning, catalytic incineration, and direct combustion.
  • Korean Patent Application No. 10-1996-0025606 discloses a microbial composition for the biological treatment of food waste, comprising thermoresistant filamentous fungi adapted to high temperatures, thermophilic bacillus bacteria which degrade proteins and lipids, and thermoresistant actinomyces; and a method for composting food waste.
  • Korean Patent No. 292879 (filed on April 7, 1999) pertains to a microorganism composition and a method for the treatment of livestock excretions using the same.
  • the microorganism composition comprises a bacillus species ⁇ Bacillus subtilis KCTC 1028) which is highly capable of producing an enzyme useful in the degradation of carbohydrates and proteins, and a photosynthetic microorganism (Rhodopseudomonas spp., deposited in the Korean Collection for Type Cultures with the accession number of KCTC 8937P) which can grow on the lysates and deodorize livestock excretions.
  • the use of microorganism agents offers an improvement in growth conditions for livestock and living environments for stock farmers .
  • Korean Patent No. 378667 discloses a composition and a method for deodorizing and composting livestock excretions.
  • the composition is used to fermentatively degrade livestock excretions or food waste into organic manure, thereby removing the nasty odor of livestock excretions and the smell generated at an early putrefaction stage of food waste.
  • the deodorizing composition is prepared by aerobically culturing at least one species selected from the group consisting of Streptomyces spp., Bacillus spp., Micrococcus spp., Achromobacter spp., Flavobacterium spp., Escherichia spp. and Pseudomonas spp. in a general broth such as nutrient broth or TSB broth, adsorbing the culture onto a medium selected from among limestone, bentonite and humus soil, and drying it.
  • Korean Patent No. 433268 discloses a microbial composition for the biological treatment of malodorants and a method for the preparation thereof.
  • the microbial composition comprises deodorizing microorganisms composed of a thermoresistant filamentous fungal species adapted to organic matter and high temperatures; another filamentous fungal species adapted to substrate change; thermoresistant and thermophilic bacillus capable of growing on a broad range of substrates; and thermoresistant Actinomyces adapted to a high concentration of organic substances.
  • thermoresistant filamentous fungal species is at least one microorganism selected from the group consisting of Aspergillus niger, Aspergillus oryzae, Penicillium spp., Mucor spp., Trichoderma viride, Cephallosporium acremonium, and Issatchenkia orientalis.
  • the filamentous fungal species adapted to substrate change is selected from among Candida spp., Arthrobacter spp., and Nocardia spp.
  • thermoresistant and thermophilic bacillus capable of growing on a broad range of substrates may be Bacillus subtilis CH-IO, Bacillus mesentericus, Bacillus megaterium, Bacillus licheniformmis NLRI-33, Bacillus thuringiensis, Bacillus circulans or a combination thereof.
  • the thermophilic actinomyces adapted to high concentrations of organic substances is Thermoactinomyces spp. and/or Thermomonspora spp.
  • the thermoresistant actinomyces adaptable to high concentrations of organic substances is selected from among Streptomyces griseus, Streptomyces globisporius, Streptomyces flavus and a combination thereof.
  • the bacterial species able to grow on a broad range of substrates is described to be preferably selected from the group consisting of Pseudomonas putida NLRI-X50, Corynebacterium spp., Staphylococcus lentus, Micrococcus lylae, and a combination thereof.
  • Korean Patent Application No. 10-2005-0044120 (filed on May 25, 2005) presents an air cleaning apparatus for removing stench and sand dust from inside a barn, with the application of Bacillus subtilis for deodorization.
  • Korean Patent Application No. 10-2006-0032543 (filed
  • Korean Patent Application No. 10-2004-0011349 suggests a Geotrichum-immobilized biofilter and a method for removing methylethylketone using the same, disclosing that the removal of methylethylketone with the Geotrichum-immobilized biofilter requires a lower cost than do conventional methods using, for example, active carbon or incineration, and does not produce nitrogen oxides or secondary pollutants.
  • Korean Patent Application No. 10-2003-0078402 (filed Nov. 6, 2003) provides a biofilter system for the removal of malodor gases and volatile organic compounds, with the application of Pseudomonas spp. to the removal of volatile oirganic compounds .
  • the present invention provides a novel strain Bacillus clausii BC7 KCCM 10863P, which has the ability to deodorize livestock wastewater and food waste faster and more effectively than conventional strains, and to remove heavy metals.
  • the present invention provides a microorganism composition for removing nasty odor and heavy metals, comprising the novel strain Bacillus clausii BC7 KCCM 10863P as an active ingredient.
  • the novel strain Bacillus clausii BC7 KCCM 10863P can greatly reduce the nasty odor of livestock wastewater or food waste. Further, this microorganism can remove heavy metals from livestock wastewater or food waste. Having such abilities, the microorganism composition according to the present invention may be applied for the clarification of livestock wastewater and food waste. Further, it can be used to prepare organic liquefied fertilizer from livestock wastewater and prepare animal feed from food waste.
  • FIG. 1 is a schematic diagram showing a device used in the experiments to determine the ability to deodorize livestock wastewater.
  • FIG. 2 is a schematic diagram showing a device used in the experiments to determine the ability to deodorize food waste. [Best Mode]
  • the present invention provides a novel bacterial strain, identified as Bacillus clausii BC7 KCCM 10863P, which is useful in d €:odorization and heavy metal removal.
  • the present invention provides a microbial composition for the removal of malodor and heavy metals, comprising an effective amount of the novel strain Bacillus clausii BC7
  • the bacterial strain may be formulated into a solid preparation, such as beads, or a liquid preparation.
  • Solid preparations suffer from the disadvantage of causing an inhomogeneous distribution of the active ingredient therein, decreasing microbial activity and leading to a reduction in the applicability thereof, and generating dust during the manufacture thereof.
  • liquid preparations have advantages over the solid preparations in that they can elicit microbial activity faster and are convenient for treating, but are inferior to solid preparations in terms of preservability.
  • a method comprising: (a) dropping a mixture of a 0.1-5.0 wt% solution of a negatively charged biodegradable polymer, such as alginate, pectin, c ⁇ irrageenan or polyaspartic acid, and a Bacillus clausii BC7 KCCM 10863P culture into a 0.5-3.0 wt% solution containing a metal salt such as Ca 2+ , Cu 2+ , Zn 2+ , Ni 2+ , Co 2+ , Mn 2+ , Al 2+ , Fe 2+ , or Mg 2+ to form beads; and (b) electrostatistically coating the beads with a 0.1-3.0 wt% solution of a positively charged biodegradable polymer such as chitosan, a chitosan derivative or polylysine by stirring the beads within the solution for 0.5 - 2 hrs.
  • a positively charged biodegradable polymer such as chitosan, a chitos
  • the coated beads may be used. Alternatively, they may be subjected to additional processes for improving the survival rate and activity of the microorganism, including (c) washing the beads with 50 mM sodium acetate (pH 5.5) to remove an excess of the positively charged biodegradable polymer; (d) stirring the bead within a 0.1 - 2.0 wt% solution of a negatively charged polymer; and (e) washing the beads with saline and stirring them within a 0.1 - 2.0 wt% solution of a positively charged biodegradable polymer to form a thicker outer film thereon.
  • additional processes for improving the survival rate and activity of the microorganism including (c) washing the beads with 50 mM sodium acetate (pH 5.5) to remove an excess of the positively charged biodegradable polymer; (d) stirring the bead within a 0.1 - 2.0 wt% solution of a negatively charged polymer; and (e) washing the beads with saline and stirring them within a 0.1 -
  • the microbial composition for the removal of malodor and heavy metals in accordance with another embodiment of the present invention comprises the novel strain Bacillus clausii BC7 KCCM 10863P in a sufficiently effective amount to conduct the above-mentioned functions, in combination with one or more Bacillus strain selected from the group consisting of Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 1086OP, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC ⁇ KCCM 10862P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P.
  • the present invention provides a method for reducing malodor or heavy metals of food waste or livestock wastewater, comprising culturing the novel strain Bacillus clausii BC7 KCCM 10863P with malodorous food waste or livestock wastewater.
  • food waste and/or livestock wastewater are deodorized and/or depossessed of heavy metals using a method in which a microbial composition comprising a sufficiently effective amount of the novel strain Bacillus clausii BC7 KCCM 10863P, in combination with one or more Bacillus strain selected from the group consisting of Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 1086OP, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P, and a
  • the present invention provides a method for composting livestock wastewater into organic liquefied fertilizer and transforming food waste into animal feeds using a sufficiently effective amount of the novel strain Bacillus clausii BC7 KCCM 10863P.
  • organic liquefied fertilizer for example, it may be prepared from livestock wastewater using peat.
  • a conventional method comprises pulverizing and mixing peat, organic materials, oyster and coal ash at a proper ratio, adding sodium hydrogen carbonate, calcium oxide and calcium chloride to the mixture in effective amounts to achieve deodorization, extracting organic liquefied fertilizer, neutralizing the extracted organic liquefied fertilizer with diluted hydrochloric acid, and aerating the neutralized organic liquid to decrease the chemical oxygen demand thereof.
  • a dry fermentation method, a mixed fermentation method, a combination thereof, or a liquid- phase fermentation method may be provided for the preparation of animal feeds from food waste.
  • the dry fe:rmentation method greatly reduces the food waste in mass, but demands an inordinate amount of energy for drying and produces nutritionally insufficient feeds which thus cannot be used per se. Further, the resulting feeds are difficult to pack and apply to an automatic feeding line due to the high water content (around 40%) thereof.
  • the mixed fermentation method allows the animal feeds to be balanced nutritionally, but is unable to produce various animal feeds meeting the demand for different nutrient contents according to age.
  • the use of mixed and dry fermentation methods in combination provides ease in maintaining a nutritional balance and adjusting water content in the resulting animal feed, but requires high production cost because expense must be paid for drying and water content adjustment.
  • the liquid-phase fermentation method typically resulting in an animal feed with a water content of around 85%, has an advantage over the other methods in terms of piroduction cost, but the products are inconvenient to transport .
  • the present invention provides a method for producing organic liquefied fertilizer, from livestock wastewater, and animal feeds, from food waste, using a microbial composition comprising an effective amount of Bacillus clausii BC7 KCCM 10863P and one or more Bacillus strain selected from the group consisting of Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 10860P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC ⁇ KCCM 10862P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P, and a combination thereof.
  • Bacillus clausii BC7 KCCM 10863P the principle of the present invention, was taken from mushrooms and hyphae grown on fallen leaves in a forest located in Samgi, Iksan,
  • the mushrooms and hyphae were immersed in a culture medium comprising 5 g of black sugar and 300 ml of water in a 500 ml beaker before incubation at 28 - 30 0 C. It was found that the nasty odor ceased in 24 hours after the application of a portion of the culture to a sewage treatment tank, showing the deodorizing ability of the culture. When the culture was maintained in molasses and applied to food waste, it was also found to be highly effective in deodorizing food waste. From the culture, useful microorganisms were isolated. In a microbiology lab of Kunsan National University,
  • Bacillus clausii BC7 KCCM 10863P was added alone or in combination with Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 1086OP, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P to microorganism reaction vessels containing livestock wastewater (see FIG.
  • An absorption bottle equipped with a glass frit impactor having a capacity of 20 ml or greater was used.
  • test sample solution was mixed with 5 ml of phenol pentacyanonitrosyl ferrate (III) solution by a ⁇ jitation and then with 5 ml of a sodium hypochlorite solution and was allowed to stand for 1 hour at 25 ⁇ 30 0 C, followed by measuring absorbance at 640 run.
  • 10 mL of the collected solution (absorption solution) was used in the same manner as in the test sample solution.
  • a test sample solution prepared by absorbing a sample into an aqueous sulfate solution (359+1) was partially teiken with a volumetric syringe and injected into a silicon stopper-plugged degradation bottle containing a potassium hydroxide solution.
  • the solution was bubbled by injecting 2 ⁇ 3 L of nitrogen at a rate of 0.2 ⁇ 0.3 L/min to generate trimethyl amine which was then concentrated at a low temperature in a condenser.
  • Trimethyl amine was desorbed by heating the concentration to a temperature of 70 0 C and introduced into a column for FID analysis.
  • a sample was passed through a sample collection tube filled with 2.4-dinitrophenyl hydrazine-coated octadecylsilylated silica gel. After the evaporation of acetonitrile from the sample collection tube, aldehydes captured by the collection tube were dissolved in ethyl acetate and a portion thereof was introduced into HPLC
  • Table 1 were summarized the analysis results obtained after culturing Bacillus clausii BC7 KCCM 10863P alone and in combination with other Bacillus strains for 4 days in w£istewater.
  • Bacillus clausii BC7 KCCM 10863P showed excellent ability to remove ammonia, hydrogen sulfide, methyl mercaptan, dimethyl disulfide, trimethyl amine and acetaldehyde. However, this bacteria was found to be unable to remove dimethyl sulfide as similar concentrations were detected for dimethyl sulfide between the samples and the control.
  • Example 3 Deodorizing Effect of Bacillus clausii BC7 KCCM 10863P Alone or in Combination with other Bacillus Strains on Food Waste
  • Bacillus clausii BC7 KCCM 10863P was added alone or in combination with Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 10860P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P to microorganism reaction vessels containing food waste (see FIG.
  • Conditions for guaranteeing the microorganisms to actively grow in the reaction vessels included temperature maintenance at 37.5°C with a heating band and use of an automatic temperature controller (see FIG.2) .
  • the ingredient concentrations of the malodor of raw food waste were analyzed to be used as a monitoring reference. Two liters of food waste was placed in each reaction vessel.
  • Bacillus clausii BC7 KCCM 10863P showed excellent ability to remove ammonia, hydrogen sulfide, methyl mercaptan, dimethyl sulfide and dimethyl disulfide. However, this bacteria was found to be unable to effectively remove trimethyl amine and acetaldehyde, as similar concentrations of these chemicals were detected between the samples and the control .
  • Bacillus clausii BC7 KCCM 10863P In order to evaluate the ability of Bacillus clausii BC7 KCCM 10863P to reduce heavy metals of food waste, two liters of food waste was placed in each reaction vessel and 5 ml of a culture of Bacillus clausii BC7 KCCM 10863P and 5 ml of a mixture containing equal amounts of Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 10860P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM
  • HNO 3 , H 2 SO 4 , and HClO 4 were mixed at a ratio of 10:1:4 according to the quantity needed.
  • Bacillus clausii BC7 KCCM 10863P was found to greatly remove cadmium, copper, zinc and lead from livestock wastewater. Also, the bacteria showed a high ability to remove cadmium, copper, zinc and lead from food waste. Nickel, however, was detected to be of a higher concentration in food waste, which was contrary to the result of livestock wastewater, leaving the nickel-removing ability of Bacillus clausii BC7 KCCM 10863P unclear.
  • Bacillus clausii BC7 KCCM 10863P according to the present invention is very useful in deodorizing livestock wastewater and food waste.
  • the novel bacterial strain of the present invention is highly effective at removing the nasty odor of livestock wastewater and food waste.
  • the strain of the present invention is capable of very effectively removing heavy metals from livestock wastewater or food waste.

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Abstract

La présente invention concerne une nouvelle souche Bacillus clausii BC7 KCCM 10863P qui est hautement capable d'éliminer les mauvaises odeurs et les métaux lourds des eaux usées d'élevage et des rebuts alimentaires. Cette nouvelle souche peut s'utiliser seule ou en association avec d'autres espèces de Bacillus pour l'élimination des mauvaises odeurs et des métaux lourds. Elle peut également s'utiliser pour convertir en ressources utiles les eaux usées d'élevage ou les rebuts alimentaires.
PCT/KR2007/002221 2007-05-04 2007-05-04 Bacillus clausii bc7 10863p capable d'éliminer les mauvaises odeurs et les métaux lourds, et procédés de clarification l'utilisant pour récupérer des ressources à partir de rebuts alimentaires ou d'eaux usées d'élevage WO2008146960A1 (fr)

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PCT/KR2007/002221 WO2008146960A1 (fr) 2007-05-04 2007-05-04 Bacillus clausii bc7 10863p capable d'éliminer les mauvaises odeurs et les métaux lourds, et procédés de clarification l'utilisant pour récupérer des ressources à partir de rebuts alimentaires ou d'eaux usées d'élevage
KR20097025245A KR101106033B1 (ko) 2007-05-04 2007-05-04 악취제거 및 중금속 제거 바실러스 클라우시 b c7 k c c m 10863p 및 이를 이용한 축산폐수 또는 음식물쓰레기의 자원화를 위한 정화방법

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CN109647181A (zh) * 2019-02-18 2019-04-19 王玉廷 一种畜禽粪便臭味除臭剂及其制备方法
CN113621540A (zh) * 2021-08-11 2021-11-09 沈阳农业大学 克劳氏芽孢杆菌菌株及其筛选方法和应用

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KR102551904B1 (ko) * 2022-12-26 2023-07-06 (주)에코비즈넷 바실러스 리체니포미스 bs 및 이를 포함하는 악취 감소용 조성물

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CN109647181A (zh) * 2019-02-18 2019-04-19 王玉廷 一种畜禽粪便臭味除臭剂及其制备方法
CN113621540A (zh) * 2021-08-11 2021-11-09 沈阳农业大学 克劳氏芽孢杆菌菌株及其筛选方法和应用
CN113621540B (zh) * 2021-08-11 2023-01-06 沈阳农业大学 克劳氏芽孢杆菌菌株及其筛选方法和应用

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