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WO2008136545A1 - Bacillus licheniformis bc4 kccm 10860p réduisant les mauvaises odeurs et les métaux lourds et procédés de clarification pour récupérer des ressources de déchets alimentaires ou des eaux usées de l'élevage l'utilisant - Google Patents

Bacillus licheniformis bc4 kccm 10860p réduisant les mauvaises odeurs et les métaux lourds et procédés de clarification pour récupérer des ressources de déchets alimentaires ou des eaux usées de l'élevage l'utilisant Download PDF

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Publication number
WO2008136545A1
WO2008136545A1 PCT/KR2007/002223 KR2007002223W WO2008136545A1 WO 2008136545 A1 WO2008136545 A1 WO 2008136545A1 KR 2007002223 W KR2007002223 W KR 2007002223W WO 2008136545 A1 WO2008136545 A1 WO 2008136545A1
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WIPO (PCT)
Prior art keywords
kccm
bacillus
food waste
licheniformis
bacillus licheniformis
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PCT/KR2007/002223
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English (en)
Inventor
Gun Ho Choi
Kil Ho Choi
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Pyung Kang Special Vehicle Co., Ltd.
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Publication date
Application filed by Pyung Kang Special Vehicle Co., Ltd. filed Critical Pyung Kang Special Vehicle Co., Ltd.
Priority to PCT/KR2007/002223 priority Critical patent/WO2008136545A1/fr
Priority to KR1020097025234A priority patent/KR101106037B1/ko
Publication of WO2008136545A1 publication Critical patent/WO2008136545A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Definitions

  • the present invention relates to a novel rru croorganism species useful for reducing nasty odors and heavy metals in livestock wastewater and food waste. Also, the present invention is concerned with the use of this novel microorganism in converting livestock wastewater and food waste into useful resources. In detail, the present invention relates to a novel microorganism species capable of removing nasty odors and heavy metals from livestock wastewater and food waste, and to a method for the production of organic liquid fertilizer from livestock wastewater, and to a method for the production of animal feseds from food waste. [Background Art]
  • the excretions from a body of livestock generate nasty odors, which also permeate the stalls where they are bred.
  • the nasty odor is harmful to humans and animals.
  • the nasty odor is attributed, for the most part, to livestock feces, which can be divided, by microorganisms, into easily degradable matter such as carbohydrates, starch, proteins and cellulose, and sparingly degradable matter such as lipids, lignin, etc.
  • methyl mercaptan provides an odor of rotten onions .
  • Hydrogen sulfide is an irritative, suffocative and colorless gas, which also gives off the smell of rotten onions.
  • Ammonia an irritative and colorless gas, is responsible for the characteristic urine smell. In combination, they give out a complex foul smell, disgusting persons and inhibiting the growth of the livestock.
  • Oxygenation oxygenates and degrades malodor ingredients and sterilizes bacteria, fungi and viruses so as to fundamentally remove odor resources.
  • Many methods have been suggested for oxygenation.
  • Most chemical oxygenation methods use chlorine dioxide, sodium hypochlorite, or chlorate dioxide. However, these compounds, containing chlorine may be injurious to humans and animals.
  • Enzymatic degradation methods are known to use deodorizer ' s prepared from vegetable extracts, but their ingredients and operation principles are not disclosed.
  • Absorption methods based on absorbents with large surface areas, such as active carbon, decrease in efficiency with time because available surface areas of the absorbents drop after being in use for a period of time.
  • Masking methods in which natural or synthetic odorous compounds are dissolved in highly volatile solvents, such as methyl alcohol, to generate a strong odor which conceals a deadly odor, can solve the nasty odor problem temporarily, but cannot provide a fundamental solution thereto.
  • ozone or UV radiation is used alone or in combination as a deodorizing agent. Ozone, although useful for sterilization and deodorization, may itself act as a pollutant.
  • a photocatalyst such as TiO 2
  • ozone a method in which a photocatalyst, such as TiO 2 , is used, in combination with ozone, to degrade odor sources.
  • a limitation is imposed on an increase in the surface area of photocatalysts, such as TiO 2 .
  • sulfides such as hydrogen sulfide (H 2 S) , methyl mercaptan (CH 3 SH) , methyl sulfide
  • BTXs including benzenes, toluene and xylene
  • VOCs volatile organic compounds
  • VOCs volatile organic chemicals
  • An incineration method produces secondary air pollution while an absorption method is economically disadvantageous due to the use of active carbon.
  • chemical methods require a low expense for the construction of facilities therefor, but a high expense for the operation thereof.
  • biological methods although suffering from the disadvantage of requiring high expenses for the construction of facilities therefor and complicated conditions for the operation thereof, are advantageous in that they are easy to maintain and manage and can be operated at low cost.
  • a biofilter method typical of biological deodorization, uses a bioreactor loaded with a carrier onto which deodorizing microorganisms are immobilized. The lowest operation cost is found in the biofilter system. Operation costs increase in the order of biofiltration, chemical cleaning, catalytic incineration, and direct combustion.
  • Korean Patent Application No. 10-1996-0025606 discloses a microbial composition for the biological treatment of food waste, comprising thermoresistant filamentous fungi adapted to high temperatures, thermophilic bacillus bacteria which degrade proteins and lipids, and thermoresistant actinomyces; and a method for composting food waste.
  • Korean Patent No. 292879 (filed on April 7, 1999) pertains to a microorganism composition and a method for the treatment of livestock excretions using the same.
  • the microorganism composition comprises a bacillus species (Bacillus subtilis KCTC 1028) which is highly capable of producing an enzyme useful in the degradation of carbohydrates and proteins, and a photosynthetic microorganism (Rhodopseudomonas spp., deposited in the Korean Collection for Type Cultures with the accession number of KCTC 8937P) which can grow on the lysates and deodorize livestock excretions.
  • a photosynthetic microorganism Rhodopseudomonas spp.
  • Korean Patent No. 378667 discloses a composition and a method for deodorizing and composting livestock excretions.
  • the composition is used to fermentatively degrade livestock excretions or food waste into organic manure, thereby removing the nasty odor of livestock excretions and the smell generated at an early putrefaction stage of food waste.
  • the deodorizing composition is prepared by aerobically culturing at least one species selected from the group consisting of Streptomyces spp., Bacillus spp., Micrococcus spp., Achromobacter spp., Flavobacterium spp., Escherichia spp. and Pseudomonas spp. in a general broth such as nutrient broth or TSB broth, adsorbing the culture onto a medium selected from among limestone, bentonite and humus soil, and drying it.
  • Korean Patent No. 433268 discloses a microbial composition for the biological treatment of malodorants and a method for the preparation thereof.
  • the microbial composition comprises deodorizing microorganisms composed of a thermoresistant filamentous fungal species adapted to organic matter and high temperatures; another filamentous fungal species adapted to substrate change; thermoresistant and thermophilic bacillus capable of growing on a broad range of substrates; and thermoresistant Actinomyces adapted to a high concentration of organic substances.
  • thermoresistant filamentous fungal species is at least one microorganism selected from the group consisting of Aspergillus niger, Aspergillus oryzae, Penicillium spp., Mucor spp., Trichoderma viride, Cephallosporium acremonium, and Issatchenkia orientalis .
  • the filamentous fungal species adapted to substrate change is selected from among Candida spp., Arthrobacter spp., and Nocardia spp.
  • thermoresistant and thermophilic bacillus capable of growing on a broad range of substrates may be Bacillus subtilis CH-IO, Bacillus mesentericus, Bacillus megaterium, Bacillus licheniformmis NLRI-33, Bacillus thuringiensis, Bacillus circulans or a combination thereof.
  • the thermophilic actinomyces adapted to high concentrations of organic substances is Thermoactinomyces spp. and/or Thermomonspora spp.
  • the thermoresistant actinomyces adaptable to high concentrations of organic substances is selected from among Streptomyces griseus, Streptomyces globisporius, Streptomyces flavus and a combination thereof.
  • the bacterial species able to grow on a broad range of substrates is described to be preferably selected from the group consisting of Pseudomonas putida NLRI-X50, Corynebacterium spp., Staphylococcus lentus, Micrococcus lylae, and a combination thereof.
  • Korean Patent Application No. 10-2005-0044120 (filed on May 25, 2005) presents an air cleaning apparatus for removing stench and sand dust from inside a barn, with the application of Bacillus subtilis for deodorization.
  • Korean Patent Application No. 10-2006-0032543 (filed
  • Korean Patent Application No. 10-2004-0011349 suggests a Geotrichum-immobilized biofliter and a method for removing methylethylketone using the same, dj sclosing that the removal of methylethylketone with the Geotrichum-immobilized biofilter requires a lower cost than do conventional methods using, for example, active carbon or incineration, and does not produce nitrogen oxides or secondary pollutants.
  • Korean Patent Application No. 10-2003-0078402 (filed Nov. 6, 2003) provides a biofilter system for the removal of malodor gases and volatile organic compounds, with the application of Pseudomonas spp. to the removal of volatile organic compounds .
  • the present invention provides a novel strain Bacillus licheniformis BC4 KCCM 10860P, which has the ability to deodorize livestock wastewater and food waste faster and more effectively than conventional strains, and to remove heavy metals.
  • the present invention provides a microorganism composition for removing nasty odor and heavy metals, comprising the novel strain B ⁇ cillus licheniformis BC4 KCCM 10860P as an active ingredient .
  • the novel strain Bacillus licheniformis BC4 KCCM 10860P can greatly reduce the nasty odor of livestock wastewater or food waste. Further, this microorganism can remove heavy metals from livestock wastewater or food waste. Having such abilities, the microorganism composition according to the present invention may be applied for the clarification of livestock wastewater and food waste. Further, it can be used to prepare organic liquefied fertilizer from livestock wastewater and prepare animal feed from food waste .
  • FIG. 1 is a schematic diagram showing a device used in the experiments to determine the ability to deodorize livestock wastewater.
  • FIG. 2 is a schematic diagram showing a device used in the experiments to determine the ability to deodorize food waste. [Best Mode]
  • the present invention provides a novel bacterial strain, identified as Bacillus licheniformis BC4 KCCM 10860P, which is useful in deodorization and heavy metal removal.
  • the present invention provides a microbial composition for the removal of ma1odor and heavy metals, comprising an effective amount of the novel strain Bacillus licheniformis BC4 KCCM 10860P.
  • Conventional techniques may be applied for the formulation of the bacterial strain Bacillus licheniformis BC4 KCCM 10860P according to the present invention.
  • the bacterial strain may be formulated into a solid preparation, such as beads, or a liquid preparation.
  • Solid preparations suffer from the disadvantage of causing an inhomogeneous distribution of the active ingredient therein, decreasing microbial activity and leading to a reduction in the applicability thereof, and generating dust during the manufacture thereof.
  • liquid preparations have advantages over the solid preparations in that they can elicit microbial activity faster and are convenient for treating, but are inferior to solid preparations m terms of preservability .
  • a method comprising: (a) dropping a mixture of a 0.1 ⁇ 5.0 wt% solution of a negatively charged biodegradable polymer, such as alginate, pectin, carrageenan or polyaspartic acid, and a Bacillus licheniformis BC4 KCCM 10860P culture into a 0.5-3.0 wt% solution containing a metal salt such as Ca 2+ , Cu 2+ , Zn 2+ , Ni 2+ , Co 2+ , Mn 2+ , Al 2+ , Fe 2+ , or Mg 2+ to form beads; and (b) e Lectrostatistically coating the beads with a 0.1-3.0 wt% solution of a positively charged biodegradable polymer such as chitosan, a chitosan derivative or polylysine by stirring the beads within the solution for 0.5 ⁇ 2 hrs .
  • a positively charged biodegradable polymer such as chitosan, a
  • the coated beads may be used. Alternatively, they may be subjected to additional processes for improving the survival rate and activity of the microorganism, including (c) washing the beads with 50 mM sodium acetate (pH 5.5) to remove an excess of the positively charged biodegradable polymer; (d) stirring the bead within a 0.1 ⁇ 2.0 wt% solution of a negatively charged polymer; and (e) washing the beads with saline and stirring them within a 0.1 ⁇ 2.0 wt% solution of a positively charged biodegradable polymer to form a thicker outer film thereon.
  • additional processes for improving the survival rate and activity of the microorganism including (c) washing the beads with 50 mM sodium acetate (pH 5.5) to remove an excess of the positively charged biodegradable polymer; (d) stirring the bead within a 0.1 ⁇ 2.0 wt% solution of a negatively charged polymer; and (e) washing the beads with saline and stirring them within a 0.1 ⁇
  • the microbial composition for the removal of malodor and heavy metals in accordance with another embodiment of the present invention comprises the novel strain Bacillus licheniformis BC4 KCCM 10860P in a sufficiently effective amount to conduct the above-mentioned functions, in combination with one or more Bacillus strain selected from the group consisting of Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P.
  • the present invention provides a method for reducing malodor or heavy metals of food waste or livestock wastewater, comprising culturing the novel strain Bacillus licheniformis BC4 KCCM 10860P with malodorous food waste or livestock wastewater.
  • food waste and/or livestock wastewater are deodorized and/or depossessed of heavy metals using a method in which a microbial composition comprising a sufficiently effective amount of the novel strain Bacillus licheniformis BC4 KCCM 10860P, in combination with one or more Bacillus strain selected from the group consisting of Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P, and
  • the present invention provides a method for composting livestock wastewater into organic liquefied fertilizer and transforming food waste into animal feeds using a sufficiently effective amount of the novel strain Bacillus licheniformis BC4 KCCM 10860P.
  • the production of organic liquefied fertilizer from livestock wastewater and animal feeds from food waste may be achieved using a conventional process without the use of the novel Bacillus licheniformis BC4 KCCM 10860P.
  • organic liquefied fertilizer for example, it may be prepared from livestock wastewater using peat.
  • a conventional method comprises pulverizing and mixing peat, organic materials, oyster and coal ash at a proper ratio, adding sodium hydrogen carbonate, calcium oxide and calcium chloride to the mixture in effective amounts to achieve deodorization, extracting organic liquefied fertilizer, neutralizing the extracted organic liquefied fertilizer with diluted hydrochloric acid, and aerating the neutralized organic liquid to decrease the chemical oxygen demand thereof.
  • a dry fermentation method, a mixed fermentation method, a combination thereof, or a IJ quid-phase fermentation method may be provided for the preparation of animal feeds from food waste.
  • the dry fermentation method greatly reduces the food waste in mass, but demands an inordinate amount of energy for drying and produces nutritionally insufficient feeds which thus cannot be used per se. Further, the resulting feeds are difficult to pack and apply to an automatic feeding line due to the high water content (around 40%) thereof.
  • the mixed fermentation method allows the animal feeds to be balanced nutritionally, but is unable to produce various animal feeds meeting the demand for different nutrient contents according to age.
  • the use of mixed and dry fermentation methods m combination provides ease in maintaining a nutritional balance and adjusting water content in the resulting animal feed, but requires high production cost because expense must be paid for drying and water content adjustment.
  • the liquid-phase fermentation method typically resulting m an animal feed with a water content of around 85%, has an advantage over the other methods in terms of production cost, but the products are inconvenient to transport.
  • the present invention provides a method for producing organic liquefied fertilizer, from livestock wastewater, and animal feeds, from food waste, using a microbial composition comprising an effective amount of Bacillus licheniformis BC4 KCCM 10860P and one or more Bacillus strain selected from the group consisting of Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P, and a combination thereof.
  • Bacillus licheniformis BC4 KCCM 10860P the principle oi the present invention, was taken from mushrooms and hyphae grown on fallen leaves in a forest located in Samgi, Iksan, Korea.
  • the mushrooms and hyphae were immersed in a culture medium comprising 5 g of black sugar and 300 ml of water m a 500 ml beaker before incubation at 28 - 30 0 C. It was found that the nasty odor ceased in 24 hours after the application of a portion of the culture to a sewage treatment tank, showing the deodorizing ability of the culture.
  • the culture was maintained in molasses and applied to food waste, it was also found to be highly effective m deodorizing food waste. From the culture, useful microorganisms were isolated.
  • Bacillus megate ⁇ um BCl-I KCCM 10856P Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 10860P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P, respectively, which were all deposited in the Korean culture center of microorganisms on April 27, 2007, wj th the respective accession numbers.
  • Example 2 Deodorizing Effect of Bacillus l ⁇ chenifoxm ⁇ s BC4 KCCM 10860P Alone or in Combination with other Bacillus Strains on Livestock Wastewater
  • Bacillus licheniformis BC4 KCCM 10860P was added alone or in combination with Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 10860P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P to microorganism reaction vessels containing livestock wastewater (
  • Conditions for guaranteeing the microorganisms to actively grow in the reaction vessels included aeration with aerator and temperature maintenance at 37.5°C with a heating band and an automatic temperature controller (see FIG.l) .
  • the malodor of raw livestock wastewater was analyzed for ingredient concentrations to be used as monitoring reference. Two liters of wastewater was placed in each reaction vessel.
  • test sample solution was mixed with 5 ml of phenol pentacyanonitrosyl ferrate (III) solution by agitation and then with 5 ml of a sodium hypochlorite solution and was allowed to stand for 1 hour at 25 ⁇ 30 0 C, followed by measuring absorbance at 640 nm.
  • 10 mL of the collected solution was used in the same manner as in the test sample solution.
  • a sample gas was adsorbed and concentrated at -170 0 C with an SPT (Sample Preconcentration Trap) , desorbed by heating, and injected into gas chromatography equipped with a PFPD (Pulse Flame Photometric Detector) .
  • SPT Sample Preconcentration Trap
  • PFPD Pulse Flame Photometric Detector
  • a test sample solution prepared by absorbing a sample into an aqueous sulfate solution (359+1) was partially taken with a volumetric syringe and injected into a silicon stopper-plugged degradation bottle containing a potassium hydroxide solution.
  • the solution was bubbled by injecting 2 ⁇ 3 L of nitrogen at a rate of 0.2 ⁇ 0.3 L/min to generate t ⁇ methyl amine which was then concentrated at a low temperature in a condenser.
  • Trimethyl amine was desorbed by heating the concentration to a temperature of 70 0 C and introduced into a column for FID analysis.
  • a sample was passed through a sample collection tube filled with 2.4-dmitrophenyl hydrazme-coated octadecylsilylated silica gel. After the evaporation of acetonitrile from the sample collection tube, aldehydes captured by the collection tube were dissolved in ethyl acetate and a portion thereof was introduced into HPLC (Young Lin Instrument Co. Ltd.) and analyzed.
  • HPLC Young Lin Instrument Co. Ltd.
  • Table 1 were summarized the analysis results obtained after culturing Bacillus licheniformis BC4 KCCM 10860P alone and in combination with other Bacillus strains for 4 days m wastewater.
  • Bacillus licheniformis BC4 KCCM 10860P showed excellent ability to remove ammonia, hydrogen sulfide, methyl mercaptan, dimethyl .disulfide, trimethyl amine and acetaldehyde. However, this bacteria was found to be unable to remove dimethyl sulfide as similar concentrations were detected for dimethyl sulfide between the samples and the control.
  • Example 3 Deodorizing Effect of Bacillus l ⁇ cheniformis BC4 KCCM 10860P Alone or in Combination with other Bacillus Strains on Food Waste
  • Bacillus licheniformis BC4 KCCM 10860P was added alone or in combination with Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 10860P, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus clausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9 KCCM 10865P and Bacillus cereus BClO KCCM 10866P to microorganism reaction vessels containing food waste (see
  • Conditions for guaranteeing the microorganisms to actively grow in the reaction vessels included temperature maintenance at 37.5°C with a heating band and use of an automatic temperature controller (see FIG.2) .
  • Bacillus licheniformis BC4 KCCM 10860P showed excellent ability to remove ammonia, methyl mercaptan, dimethyl sulfide, dimethyl disulfide and trimethyl amine. However, this bacteria was found to be unable to effectively remove hydrogen sulfide and acetaldehyde, as similar concentrations of these chemicals were detected between the samples and the control.
  • Bacillus licheniformis BC4 KCCM 10860P In order to evaluate the ability of Bacillus licheniformis BC4 KCCM 10860P to reduce heavy metals of food waste, two liters of food waste was placed in each reaction vessel and 5 ml of a culture of Bacillus licheniformis BC4 KCCM 10860P and 5 ml of a mixture containing equal amounts of Bacillus megaterium BCl-I KCCM 10856P, Bacillus megaterium BCl-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus cereus BC3 KCCM 10859P, Bacillus licheniformis BC4 KCCM 1086OP, Bacillus cereus BC5 KCCM 10861P, Bacillus sphaericus BC6 KCCM 10862P, Bacillus dausii BC7 KCCM 10863P, Bacillus licheniformis BC8 KCCM 10864
  • Bacillus licheniformis BC4 KCCM 10860P was found to greatly remove cadmium, copper, zinc and lead from livestock wastewater. Also, the bacteria showed a high ability to remove cadmium, copper, zinc and lead from food waste. Nickel and chromium, however, was detected to be of a higher concentration in food waste, which was contrary to the result of livestock wastewater, leaving the nickel and chromium-removing ability of Bacillus licheniformis BC4 KCCM 10860P unclear, [industrial Applicability]
  • Bacillus licheniformis BC4 KCCM 10860P according to the present invention is very useful in deodorizing livestock wastewater and food waste.
  • the novel bacterial strain of the present invention is highly effective at removing the nasty odor of livestock wastewater and food waste.
  • the strain of the present invention is capable of very effectively removing heavy metals from livestock wastewater or food waste.

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  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Sludge (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

L'invention concerne une nouvelle souche de Bacillus licheniformis BC4 KCCM 1086OP qui est particulièrement capable d'éliminer les mauvaises odeurs et les métaux lourds des eaux usées de l'élevage et des déchets alimentaires. Cette nouvelle souche peut être utilisée seule ou en combinaison avec d'autres espèces de Bacillus pour éliminer les mauvaises odeurs et le métaux lourds. On peut également l'utiliser pour transformer les eaux usées de l'élevage ou des déchets alimentaires en ressources utiles.
PCT/KR2007/002223 2007-05-04 2007-05-04 Bacillus licheniformis bc4 kccm 10860p réduisant les mauvaises odeurs et les métaux lourds et procédés de clarification pour récupérer des ressources de déchets alimentaires ou des eaux usées de l'élevage l'utilisant WO2008136545A1 (fr)

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PCT/KR2007/002223 WO2008136545A1 (fr) 2007-05-04 2007-05-04 Bacillus licheniformis bc4 kccm 10860p réduisant les mauvaises odeurs et les métaux lourds et procédés de clarification pour récupérer des ressources de déchets alimentaires ou des eaux usées de l'élevage l'utilisant
KR1020097025234A KR101106037B1 (ko) 2007-05-04 2007-05-04 악취제거 및 중금속 제거 바실러스 라이케니포미스 b c4 k c c m 10860p 및 이를 이용한 축산폐수 또는 음식물쓰레기의 자원화를 위한 정화방법

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WO2012066749A1 (fr) * 2010-11-15 2012-05-24 Toyota Jidosha Kabushiki Kaisha Micro-organisme et déodorant le contenant
CN108977398A (zh) * 2018-09-13 2018-12-11 成都友益佳环保设备工程有限公司 一株巨大芽孢杆菌及其应用
JP2019076006A (ja) * 2017-10-23 2019-05-23 ジェックス株式会社 混合バクテリア
CN112442465A (zh) * 2020-12-08 2021-03-05 北京嘉博文生物科技有限公司 一种复合微生物除臭菌剂及其制备方法和应用
CN113980840A (zh) * 2021-10-15 2022-01-28 辽宁农业职业技术学院 一种用于公共环境的微生物除臭液及其制备方法

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KR102661505B1 (ko) * 2021-09-17 2024-05-07 주식회사 평강비아이엠 고염도 어류 폐기물을 이용한 사료제조방법
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WO2012066749A1 (fr) * 2010-11-15 2012-05-24 Toyota Jidosha Kabushiki Kaisha Micro-organisme et déodorant le contenant
CN103221532A (zh) * 2010-11-15 2013-07-24 丰田自动车株式会社 微生物和含有其的除臭剂
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JP2019076006A (ja) * 2017-10-23 2019-05-23 ジェックス株式会社 混合バクテリア
CN108977398A (zh) * 2018-09-13 2018-12-11 成都友益佳环保设备工程有限公司 一株巨大芽孢杆菌及其应用
CN108977398B (zh) * 2018-09-13 2021-10-08 成都友益佳环保设备工程有限公司 一株巨大芽孢杆菌及其应用
CN112442465A (zh) * 2020-12-08 2021-03-05 北京嘉博文生物科技有限公司 一种复合微生物除臭菌剂及其制备方法和应用
CN113980840A (zh) * 2021-10-15 2022-01-28 辽宁农业职业技术学院 一种用于公共环境的微生物除臭液及其制备方法

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