WO2008134069A3 - Anchored ligand agents - Google Patents
Anchored ligand agents Download PDFInfo
- Publication number
- WO2008134069A3 WO2008134069A3 PCT/US2008/005528 US2008005528W WO2008134069A3 WO 2008134069 A3 WO2008134069 A3 WO 2008134069A3 US 2008005528 W US2008005528 W US 2008005528W WO 2008134069 A3 WO2008134069 A3 WO 2008134069A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ligand
- target
- target molecule
- binding
- active site
- Prior art date
Links
- 239000003446 ligand Substances 0.000 title abstract 6
- 239000003795 chemical substances by application Substances 0.000 title 1
- 108091023037 Aptamer Proteins 0.000 abstract 2
- 108020004635 Complementary DNA Proteins 0.000 abstract 1
- 108090000190 Thrombin Proteins 0.000 abstract 1
- 239000003146 anticoagulant agent Substances 0.000 abstract 1
- 229940127090 anticoagulant agent Drugs 0.000 abstract 1
- 239000000729 antidote Substances 0.000 abstract 1
- 229940075522 antidotes Drugs 0.000 abstract 1
- 239000008364 bulk solution Substances 0.000 abstract 1
- 238000010804 cDNA synthesis Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 238000010494 dissociation reaction Methods 0.000 abstract 1
- 230000005593 dissociations Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940124597 therapeutic agent Drugs 0.000 abstract 1
- 229960004072 thrombin Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/50—Methods for regulating/modulating their activity
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides novel therapeutic agents comprising ligand molecules such as aptamers linked to a molecular anchor (MA). In the absence of a MA, ligands rapidly diffuse into bulk solution after dissociation from a target molecule. With a MA tightly bound to the target molecule, anchored ligand molecules of the invention remain in close proximity to the active site on the target molecule, even after dissociating from the target, permitting rapid re- binding of ligand to its target. Reversibility of binding to the active site can be effectively and rapidly achieved using antidotes based on the complementary DNA sequences. A preferred embodiment in accordance with the invention is a highly efficient anticoagulant agent that demonstrates binding efficiency to thrombin that is increased many fold relative to a corresponding unanchored aptamer-based ligand.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US91424207P | 2007-04-26 | 2007-04-26 | |
| US60/914,242 | 2007-04-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2008134069A2 WO2008134069A2 (en) | 2008-11-06 |
| WO2008134069A3 true WO2008134069A3 (en) | 2009-02-12 |
Family
ID=39926277
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2008/005528 WO2008134069A2 (en) | 2007-04-26 | 2008-04-28 | Anchored ligand agents |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2008134069A2 (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5756291A (en) * | 1992-08-21 | 1998-05-26 | Gilead Sciences, Inc. | Aptamers specific for biomolecules and methods of making |
| US20020037506A1 (en) * | 2000-04-18 | 2002-03-28 | Yun Lin | Aptamer based two-site binding assay |
| US20030175703A1 (en) * | 2000-09-26 | 2003-09-18 | Sullenger Bruce A. | RNA aptamers and methods for identifying the same |
| US20050123936A1 (en) * | 2003-01-16 | 2005-06-09 | Ansari Aseem Z. | Method, composition, and kit to design, evaluate, and/or test compounds that modulate regulatory factor binding to nucleic acids |
| WO2007025049A2 (en) * | 2005-08-26 | 2007-03-01 | Archemix Corp. | Aptamers that bind thrombin with high affinity |
-
2008
- 2008-04-28 WO PCT/US2008/005528 patent/WO2008134069A2/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5756291A (en) * | 1992-08-21 | 1998-05-26 | Gilead Sciences, Inc. | Aptamers specific for biomolecules and methods of making |
| US20020037506A1 (en) * | 2000-04-18 | 2002-03-28 | Yun Lin | Aptamer based two-site binding assay |
| US20030175703A1 (en) * | 2000-09-26 | 2003-09-18 | Sullenger Bruce A. | RNA aptamers and methods for identifying the same |
| US20050123936A1 (en) * | 2003-01-16 | 2005-06-09 | Ansari Aseem Z. | Method, composition, and kit to design, evaluate, and/or test compounds that modulate regulatory factor binding to nucleic acids |
| WO2007025049A2 (en) * | 2005-08-26 | 2007-03-01 | Archemix Corp. | Aptamers that bind thrombin with high affinity |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008134069A2 (en) | 2008-11-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PH12018501850A1 (en) | Antigen-binding molecule capable of binding to two or more antigen molecules repeatedly | |
| WO2008068637A3 (en) | Ob-fold used as scaffold for engineering new specific binders | |
| WO2009111508A3 (en) | Metalloproteinase 9 and metalloproteinase 2 binding proteins | |
| WO2008083327A3 (en) | Enrichment through heteroduplexed molecules | |
| IL196732A0 (en) | Antigen binding molecules that bind egfr, vectors encoding same, and uses thereof | |
| IL197061A (en) | Compounds inhibiting the interaction of bcl proteins with binding partners, pharmaceutical compositions comprising them and their use | |
| WO2009132020A3 (en) | Selective high-affinity polydentate ligands and methods of making such | |
| EA200801930A1 (en) | BISPECIFIC MOLECULE, BINDING TLR9 AND CD32 AND CONTAINING T-CELL EPITOPE FOR THE TREATMENT OF ALLERGIES | |
| WO2008125623A3 (en) | Molecules and methods for modulating proprotein convertase subtilisin/kexin type 9 (pcsk9) | |
| IL178761A0 (en) | Antibodies and molecules derived therefrom that bind to steap-1 proteins | |
| WO2012035050A3 (en) | Factor viii variants having a decreased cellular uptake | |
| WO2010040772A3 (en) | Targeting construct comprising anti-polymer antibody and liposomes or microvesicles binding to the same | |
| WO2008036929A3 (en) | Complex for transferring an anionic substance into a cell | |
| WO2012162547A3 (en) | Long duration dual hormone conjugates | |
| WO2011028883A3 (en) | Metalloproteinase 9 and metalloproteinase 2 binding proteins | |
| WO2007026972A3 (en) | Binding protein molecule | |
| WO2008122088A8 (en) | Methods for detecting a target nucleotide sequence in a sample utilising a nuclease-aptamer complex | |
| WO2011131510A3 (en) | Selective modification of proteins | |
| WO2009097397A3 (en) | Metalloproteinase binding proteins | |
| WO2008134069A3 (en) | Anchored ligand agents | |
| WO2008007960A3 (en) | Gainac specific binding molecules and uses thereof | |
| WO2013059439A3 (en) | Combination therapy comprising an mmp-14 binding protein | |
| WO2009002228A3 (en) | New thrombin function compounds and pharmaceutical compositions based on them | |
| WO2005000870A3 (en) | Isolated ny-eso-1 peptides which bind to hla class ii molecules and uses thereof | |
| WO2008124134A3 (en) | High-pressure refolding of proteins in the presence of binding partners |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 08743408 Country of ref document: EP Kind code of ref document: A2 |
|
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| 122 | Ep: pct application non-entry in european phase |
Ref document number: 08743408 Country of ref document: EP Kind code of ref document: A2 |