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WO1998000521A1 - Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives - Google Patents

Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives Download PDF

Info

Publication number
WO1998000521A1
WO1998000521A1 PCT/JP1997/002167 JP9702167W WO9800521A1 WO 1998000521 A1 WO1998000521 A1 WO 1998000521A1 JP 9702167 W JP9702167 W JP 9702167W WO 9800521 A1 WO9800521 A1 WO 9800521A1
Authority
WO
WIPO (PCT)
Prior art keywords
serum
cells
medium
physiologically active
free medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP1997/002167
Other languages
English (en)
Japanese (ja)
Inventor
Yasuo Amatsuji
Hiroshi Tamashima
Yahiro Uemura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
GC Biopharma Corp
Original Assignee
Green Cross Corp Japan
Green Cross Corp Korea
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan, Green Cross Corp Korea filed Critical Green Cross Corp Japan
Publication of WO1998000521A1 publication Critical patent/WO1998000521A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components

Definitions

  • ⁇ ⁇ ⁇ May be a source of contamination such as viruses, mycoplasmas, or prions.
  • a second object of the present invention is to provide a method for efficiently culturing animal cells.
  • a third object of the present invention is to provide a method for producing a physiologically active substance in a large amount from animal cells capable of expressing and producing the physiologically active substance.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne: des milieux exempts de sérum caractérisés par le fait qu'ils contiennent de l'insuline et de la peptone tout en étant pratiquement exempts d'albumine et de transferrine; une méthode de culture de cellules d'origine animale caractérisée par le fait que l'on fait croître les cellules dans les milieux susmentionnés; et un procédé de production de substances physiologiquement actives caractérisé en ce qu'on procède à la culture de cellules d'origine animale, pouvant produire lesdites substances, dans les milieux susmentionnés et que l'on récupère les substances ainsi produites dans les cultures. Les milieux exempts de sérum présentés permettent une prolifération suffisante des cellules, facilitent la récupération des substances physiologiquement actives dans les cultures, et ils sont rarement contaminés, permettant ainsi la mise en oeuvre de la méthode de culture et du procédé de production susmentionnés.
PCT/JP1997/002167 1996-06-28 1997-06-23 Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives Ceased WO1998000521A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP8/168759 1996-06-28
JP16875996 1996-06-28

Publications (1)

Publication Number Publication Date
WO1998000521A1 true WO1998000521A1 (fr) 1998-01-08

Family

ID=15873915

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1997/002167 Ceased WO1998000521A1 (fr) 1996-06-28 1997-06-23 Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives

Country Status (1)

Country Link
WO (1) WO1998000521A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002529072A (ja) * 1998-11-06 2002-09-10 スターンベルド バイオテクノロジー ノース アメリカ， インコーポレイテッド 組換えヒトエリスロポイエチンを産生する細胞の大量培養のための方法
JP2010273683A (ja) * 2003-03-03 2010-12-09 Glaxosmithkline Biologicals Sa 動物を含まない細胞培養方法
JP2014100148A (ja) * 2007-12-27 2014-06-05 Baxter Internatl Inc 細胞培養プロセス

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03180175A (ja) * 1989-12-07 1991-08-06 Snow Brand Milk Prod Co Ltd 無血清培地
JPH04234982A (ja) * 1990-12-27 1992-08-24 Green Cross Corp:The 無血清培地、動物細胞の培養方法および異種蛋白質の製造方法
JPH05207874A (ja) * 1992-01-28 1993-08-20 Mitsui Toatsu Chem Inc 無血清培地

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03180175A (ja) * 1989-12-07 1991-08-06 Snow Brand Milk Prod Co Ltd 無血清培地
JPH04234982A (ja) * 1990-12-27 1992-08-24 Green Cross Corp:The 無血清培地、動物細胞の培養方法および異種蛋白質の製造方法
JPH05207874A (ja) * 1992-01-28 1993-08-20 Mitsui Toatsu Chem Inc 無血清培地

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002529072A (ja) * 1998-11-06 2002-09-10 スターンベルド バイオテクノロジー ノース アメリカ， インコーポレイテッド 組換えヒトエリスロポイエチンを産生する細胞の大量培養のための方法
JP2010273683A (ja) * 2003-03-03 2010-12-09 Glaxosmithkline Biologicals Sa 動物を含まない細胞培養方法
JP2014100148A (ja) * 2007-12-27 2014-06-05 Baxter Internatl Inc 細胞培養プロセス

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