WO2006113050A4 - Reactifs de detection de phosphorylation de proteines dans la voie de signalisation de carcinome - Google Patents
Reactifs de detection de phosphorylation de proteines dans la voie de signalisation de carcinome Download PDFInfo
- Publication number
- WO2006113050A4 WO2006113050A4 PCT/US2006/010868 US2006010868W WO2006113050A4 WO 2006113050 A4 WO2006113050 A4 WO 2006113050A4 US 2006010868 W US2006010868 W US 2006010868W WO 2006113050 A4 WO2006113050 A4 WO 2006113050A4
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- phosphorylated
- antibody
- column
- tyrosine
- protein
- Prior art date
Links
- 201000009030 Carcinoma Diseases 0.000 title claims abstract 7
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title abstract 2
- 230000019491 signal transduction Effects 0.000 title abstract 2
- 230000009822 protein phosphorylation Effects 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 claims abstract 26
- 102000004169 proteins and genes Human genes 0.000 claims abstract 26
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 24
- 238000000034 method Methods 0.000 claims abstract 21
- 230000026731 phosphorylation Effects 0.000 claims abstract 18
- 238000006366 phosphorylation reaction Methods 0.000 claims abstract 18
- 108091006024 signal transducing proteins Proteins 0.000 claims abstract 6
- 102000034285 signal transducing proteins Human genes 0.000 claims abstract 6
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 19
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 19
- 101150073031 cdk2 gene Proteins 0.000 claims 4
- 102000007469 Actins Human genes 0.000 claims 2
- 108010085238 Actins Proteins 0.000 claims 2
- 101150038994 PDGFRA gene Proteins 0.000 claims 2
- 102100023112 Dual specificity tyrosine-phosphorylation-regulated kinase 4 Human genes 0.000 claims 1
- 101001049983 Homo sapiens Dual specificity tyrosine-phosphorylation-regulated kinase 4 Proteins 0.000 claims 1
- DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 101710167800 Capsid assembly scaffolding protein Proteins 0.000 abstract 1
- 108010078791 Carrier Proteins Proteins 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 102000004861 Phosphoric Diester Hydrolases Human genes 0.000 abstract 1
- 108090001050 Phosphoric Diester Hydrolases Proteins 0.000 abstract 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 abstract 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 abstract 1
- 101710130420 Probable capsid assembly scaffolding protein Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102000001253 Protein Kinase Human genes 0.000 abstract 1
- 101710204410 Scaffold protein Proteins 0.000 abstract 1
- 108091005764 adaptor proteins Proteins 0.000 abstract 1
- 210000004292 cytoskeleton Anatomy 0.000 abstract 1
- 230000004060 metabolic process Effects 0.000 abstract 1
- 230000037361 pathway Effects 0.000 abstract 1
- 238000012545 processing Methods 0.000 abstract 1
- 108060006633 protein kinase Proteins 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
- 108020003175 receptors Proteins 0.000 abstract 1
- 102000005962 receptors Human genes 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 238000013519 translation Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57496—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving intracellular compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4748—Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Peptides Or Proteins (AREA)
Abstract
La présente invention concerne de nouveaux sites de phosphorylation identifiés dans des protéines de transduction de signal et dans des voies de tendance de carcinome humain et, fournit des anticorps spécifiques de sites de phosphorylation et des peptides étiquetés par des isotopes lourds (peptides AQUA) pour la détection et la quantification sélective de ces sites/protéines phosphorylés, ainsi que des techniques d'utilisation de ces réactifs à cet effet. Parmi les sites de phosphorylation identifiés, on distingue les sites survenant dans les types de protéine suivants : des protéines d'échafaudage/adaptateur, des protéines de cytosquelette, des protéine de signalisation GTP, des kinases, des protéines de métabolisme, des phosphatases/phospho-diesterases/protéases, des protéines réceptrices, des protéine de traitement ARN, des protéines de transcription, des protéines de traduction, des protéine transporteuses et des protéines d'ubiquitine, ainsi que d'autres types de protéines.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06739581A EP1872134A4 (fr) | 2005-04-12 | 2006-03-24 | Réactifs de détection de phosphorylation de protéines dans la voie de signalisation de carcinome |
| US12/985,879 US20110105732A1 (en) | 2005-04-12 | 2011-01-06 | Reagents for the Detection of Protein Phosphorylation in Carcinoma Signaling Pathways |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67044705P | 2005-04-12 | 2005-04-12 | |
| US60/670,447 | 2005-04-12 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US67044705P Continuation | 2005-04-12 | 2005-04-12 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US11/974,480 Continuation US20090099340A1 (en) | 2005-04-12 | 2007-10-12 | Reagents for the detection of protein phosphorylation in carcinoma signaling pathways |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| WO2006113050A2 WO2006113050A2 (fr) | 2006-10-26 |
| WO2006113050A8 WO2006113050A8 (fr) | 2007-12-27 |
| WO2006113050A3 WO2006113050A3 (fr) | 2008-12-24 |
| WO2006113050A4 true WO2006113050A4 (fr) | 2009-03-26 |
Family
ID=37115632
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2006/010868 WO2006113050A2 (fr) | 2005-04-12 | 2006-03-24 | Reactifs de detection de phosphorylation de proteines dans la voie de signalisation de carcinome |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP1872134A4 (fr) |
| WO (1) | WO2006113050A2 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2307541B1 (fr) * | 2008-06-23 | 2014-03-05 | PerkinElmer Health Sciences, Inc. | Substrats de kinases |
| CN111116734A (zh) * | 2019-12-23 | 2020-05-08 | 维塔恩(广州)医药有限公司 | 肿瘤相关基因c-kit突变相关抗原短肽及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2117913C (fr) * | 1992-04-10 | 2006-05-09 | Richard J. Epstein | Immunodetection de la phosphoproteine specifique de l'etat d'activation |
| AU2002307154A1 (en) * | 2001-04-06 | 2002-10-21 | Origene Technologies, Inc | Prostate cancer expression profiles |
| US7335467B2 (en) * | 2001-05-15 | 2008-02-26 | Ludwig Institute For Cancer Research | Breast cancer antigens |
-
2006
- 2006-03-24 EP EP06739581A patent/EP1872134A4/fr not_active Withdrawn
- 2006-03-24 WO PCT/US2006/010868 patent/WO2006113050A2/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| EP1872134A2 (fr) | 2008-01-02 |
| WO2006113050A8 (fr) | 2007-12-27 |
| EP1872134A4 (fr) | 2010-09-01 |
| WO2006113050A3 (fr) | 2008-12-24 |
| WO2006113050A2 (fr) | 2006-10-26 |
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